Labshake search
Citations for Qiagen :
4151 - 4200 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Using a commercial kit (QIAGEN DNeasy isolation kit #69506), DNA was isolated from a tissue punch taken from the external ear ...
-
bioRxiv - Microbiology 2023Quote: ... using a commercial kit (RT2 First Strand Kit, Qiagen). Quantification of transduction of EBECs and HBECs with the SARS-CoV-2 pseudovirus was determined by real-time qPCR (rt-PCR ...
-
bioRxiv - Microbiology 2023Quote: ... and the RNeasy kit plus Mini kit (#74134, Qiagen) or using NucleoSpin RNA Plus XS ...
-
bioRxiv - Neuroscience 2019Quote: ... anti-Strep-tag II epitope mouse monoclonal antibody # 34850 (QIAGEN), anti-6xHis-tag mouse monoclonal antibody # H1029 (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2020Quote: ... Mouse and Human IFN I RT2 Profiler PCR Arrays (Qiagen) were performed and relative expression determined using the ∆∆CT method and normalized for 5 housekeeping genes according to manufacturer’s guidance.
-
bioRxiv - Cell Biology 2021Quote: ... Frozen mouse liver were first homogenized using TissueLyser II (Qiagen) and then lysed in 1X Laemmli buffer ...
-
bioRxiv - Microbiology 2023Quote: ... blots were incubated with mouse monoclonal RGS-His antibody (Qiagen) diluted 1:1,000 ...
-
bioRxiv - Physiology 2023Quote: ... RT2 Profiler PCR Array for Mouse Fibrosis (PAMM-120Z, Qiagen) and Qiagen GeneGlobe Analysis was used to evaluate fibrosis between normal saline and experimental groups at each time point.
-
bioRxiv - Developmental Biology 2023Quote: ... RT² Profiler™ PCR Array Mouse Neurogenesis (330231, Qiagen, Germany) was performed according to the manufacturer’s instructions on LightCycler 96 (Roche ...
-
bioRxiv - Developmental Biology 2024Quote: ... mouse embryos were injected with two siRNAs (Qiagen, S104403294 & S104403287) against ERH at a total concentration of 400 nM ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We used Penta-His mouse monoclonal antibody (Qiagen, Qiagen #34660) at a 1:2000 dilution in 5% BSA in PBS-T buffer for 1 h ...
-
bioRxiv - Microbiology 2024Quote: ... and mouse anti-STREP II (QIAGEN, #34850; dilution 1:1000) antibodies were used as primary antibodies ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We used Penta-His mouse monoclonal antibody (Qiagen, Qiagen #34660) at a 1:2000 dilution in 5% BSA in PBS-T buffer for 1 h ...
-
bioRxiv - Biophysics 2021Quote: ... we first cloned the RARA coding sequence out of U2OS cDNA by extracting RNA from cycling U2OS cells with a Qiagen RNeasy kit (Qiagen 74104), preparing cDNA with the iScript Reverse Transcription Supermix (Bio-Rad 1708840) ...
-
bioRxiv - Cancer Biology 2021Quote: ... total RNA was extracted from tissues or cells as described above and reverse-transcribed using the QuantiTect Reverse Transcription Kit (Qiagen #205313). Quantitative real-time PCR (QRT-PCR ...
-
bioRxiv - Cell Biology 2019Quote: Total RNA from THP-1 cells were extracted using the RNeasy® Plus Mini Kit (Cat. No. 74134, Qiagen, Limburg, Netherlands) according to the manufacturer’s instructions at 24 and 96h p.i ...
-
bioRxiv - Cell Biology 2020Quote: ... genomic DNA of individual clones and the respective parental cell line was isolated using QIAamp DNA mini Kit (QIAGEN, Hilden, Germany) according to manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: DNA was isolated from each of the 8 samples of flies using an adaptation of the Gentra Puregene Cell Kit protocol (Qiagen, 158767). Briefly ...
-
bioRxiv - Molecular Biology 2021Quote: 2 x 107 cells were harvested by centrifugation and genomic DNA was prepared using Blood & Cell Culture DNA Midi Kit 100/G Genomic-tips (Qiagen #13343). To examine alkaline degradation 3 μg of DNA was treated with 0.3 M NaOH at 55 °C for 2 hr in 15 μl ...
-
bioRxiv - Evolutionary Biology 2020Quote: Total RNA was isolated from cell lysate by phenol::chloroform extraction and spin-column (miRNAeasy kit, Qiagen, San Diego, CA, USA), quantified by spectrophotometry and assessed for quality using the Agilent 2100 bioanalyzer (Agilent Technologies ...
-
bioRxiv - Developmental Biology 2020Quote: RNA was isolated from basal and luminal cells recovered from flow cytometry using the RNeasy Mini Kit (Qiagen, catalog no. 74104). Using the random hexamers method (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Total RNA used for the analysis of unfractionated mRNAs was extracted from the cells using the RNeasy Plus Mini Kit (QIAGEN, 74136) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... UVSS (UVSS1VI, UVSTA24) and CS (CS466VI, CS548VI, CS816VI, CS177VI) cells were isolated using the RNAeasy® Micro kit (Cat#74004; Qiagen) and mRNA were reverse transcribed with the SuperScript VI Reverse Transcriptase (Cat#18090050 ...
-
bioRxiv - Cell Biology 2022Quote: ... RNA was extracted from infected or mock-treated Caco-2 cells using the Qiagen RNAeasy Plus Extraction Kit (Qiagen, Hilden, Germany). For quantifying the SARS-CoV-2 genome abundance in mock and infected samples ...
-
bioRxiv - Genomics 2020Quote: ... Genomic DNA was purified in a 0:0.72 volumetric ratio of Agencourt AMPure XP beads and amplified using multiple displacement amplification with the REPLI-g Single Cell Kit (Qiagen #150345) to yield an average of 815ng amplified genomic DNA per clone.
-
bioRxiv - Genomics 2020Quote: ... was extracted from the cell culture pellet of each isolate using the Mo Bio Ultraclean Microbial DNA extraction kit (QIAGEN, Inc). Sequencing libraries were subsequently constructed using the KAPA HyperPlus DNA library preparation kit (Roche ...
-
bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from 32D-Cas9 cell lines expressing sgRNAs targeting Cbl introns 7 and 8 using an RNeasy Mini Kit (QIAGEN, 74106). cDNA was prepared using a QuantiTect Reverse Transcription Kit (QIAGEN ...
-
bioRxiv - Cell Biology 2019Quote: ... 3-5 million cells were then harvested and genomic DNA was isolated using QIAamp DNA mini kit (QIAGEN, prod. number 51304). The following L1 recovery steps were adapted from24,52 with modifications ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... the total RNA was extracted from the low-passage infected cell culture supernatant (500 µl) using an RNeasy Mini Kit (QIAGEN, Germany), and cDNA synthesis was carried out using an oligo d(T)15 primer and MMLV reverse transcriptase (Promega ...
-
bioRxiv - Immunology 2019Quote: Frozen samples of single cell suspensions from MLN of lab or rewilded mice were thaw to isolate RNA from approximately 106 cells by RNeasy Plus Mini Kit (QIAGEN #74136) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... Cell lysis and total RNA extractions were performed with the RNeasy mini kit according to the manufacturer’s recommendations (Qiagen, Chatsworth, CA), except that 1 mg/ml of lysozyme was used to lyse Pseudomonas cells ...
-
bioRxiv - Cell Biology 2019Quote: Total RNA was prepared from HT29 cells treated with 10 mM BG for 48 h utilizing RNeasy Mini kit (Qiagen, Germany), and was subjected to microarray and RNA sequencing analyses as described previously (Sasaki et al. ...
-
bioRxiv - Cancer Biology 2019Quote: ... Total RNA isolation and on-column DNase digestion from cells were performed basing on the manufacturer’s protocol RNeasy Plus Mini Kit (Qiagen Cat #: 74134). 1 ug of RNA was used to synthesize the cDNA according to the one step cDNA synthesis kit from QuantaBio (Cat# ...
-
bioRxiv - Microbiology 2021Quote: ... viral RNA was extracted from cells and the respective culture supernatant with the QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany). One step RT-qPCR for HCoV-NL63 with absolute virus RNA quantification was performed using the following primer/probe set as published before [22]:
-
bioRxiv - Immunology 2021Quote: ... RNA was extracted from homogenized lysate containing a range of 3×103 to 1×105 cells per sample using a ll Prep DNA/RNA Mini kit (Qiagen, #80204). RNA was purified and concentrated using RNAClean XP beads (Beckman Coulter ...
-
bioRxiv - Neuroscience 2020Quote: ... Whole genome amplification (WGA) was performed directly following FACS using the REPLI-g Advanced DNA Single Cell kit (Qiagen Cat#: 150365) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Genomic DNA was extracted from 5 million cells (∼650x representation) in the initial and final populations each using a QiaAMP DNA Blood Purification Mini Kit (Qiagen 51104) and then sgRNA sequences were amplified from each sample using a two-step PCR ...
-
bioRxiv - Cancer Biology 2020Quote: ... upwards was isolated from two different clones of pBABE-SAOS 2 and hTERT-SAOS 2 stable cell lines by using miRNeasy Mini Kit (Qiagen, #79306), following manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from leaves (not protoplasted) and leaf protoplasts isolated using the same method for the single cell sequencing using the RNeasy Kit (Qiagen, Hilden). On column DNase treatment was performed to remove residual gDNA using the RNase-free DNase kit (Qiagen ...
-
bioRxiv - Neuroscience 2020Quote: ... stem cells (H9 hESCs and iPSCs) and COs derived from H9 hESCs and iPSCs using the QIAamp DNA Mini Kit (Qiagen, 51304) by following the manufacturer’s recommended protocol and using the recommended elution volumes ...
-
bioRxiv - Systems Biology 2020Quote: ... Free DNA fragments in whole cell extract and immunoprecipitated fractions were purified by the QIAquick PCR Purification Kit (QIAGEN, Hilden, Germany) and eluted with 50 µL of elution buffer provided by the kit.
-
bioRxiv - Molecular Biology 2021Quote: ... Bacterial cells were harvested by centrifugation and the genomic DNA was extracted using the DNeasy® blood and tissue kit (Qiagen) following the manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2020Quote: ... Viral RNA was isolated from 500 μl of tissue culture supernatant of HUT-R5 cells using a QIAamp® viral RNA Mini Kit (Qiagen). RNA yield and purity were quantitated using NanoDrop™ 2000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... viral RNA was isolated from 500 μl of tissue culture supernatant from HEK293T cells using a QIAamp® viral RNA Mini Kit (Qiagen). RNA yield and purity were quantitated spectrophotometrically using NanoDrop™ 2000 ...
-
bioRxiv - Microbiology 2020Quote: ... Cultures were centrifuged for 10 minutes at 4,300 x g and the cell pellet resuspended in the lysis buffer (RLT) from the RNeasy kit (Qiagen, Germantown, MD). Cells were then homogenized with sterile 0.1 mm glass beads (Biospec Products Inc. ...
-
bioRxiv - Microbiology 2019Quote: ... total RNA was extracted using enzymatic lysis and mechanical disruption of the cells and purified with the RNeasy mini kit (Protocol 2, Qiagen, USA). The RNA standard (25ng ...
-
bioRxiv - Cell Biology 2020Quote: ... RNA was isolated from Hela cells harvested from confluent cultures according to the manufacturer’s instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA). RNA was used as a template for reverse-transcriptase polymerase chain reaction (RT-PCR ...
-
bioRxiv - Cell Biology 2020Quote: ... rKChIP3-WT (rWT) and rKChIP3-AA (rAA) cells were lysed and total RNA extracted with the RNeasy extraction kit (Qiagen, Netherlands). cDNA was synthesized with Superscript III (Invitrogen) ...
-
bioRxiv - Molecular Biology 2022Quote: RNA was extracted from liquid nitrogen-frozen cell pellets from 20 mL cultures grown to early stationary phase using the RNeasy Plant Mini Kit (Qiagen, Germany). Immediately after extraction ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA from all types of cultured osteoblasts described herein was collected using RLT Qiagen RNAeasy mini/micro kit cell lysis buffer and further purified using RNAeasy mini (Qiagen, 74134) or micro (Qiagen ...