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Citations for Qiagen :
301 - 350 of 2805 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Red blood cells were eliminated by incubation for 10 min on ice with 5 mL of erythrocyte lysis buffer (Qiagen). Human cells were enriched using the Mouse Cell Depletion Kit and the QuadroMACS magnetic cell separator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... samples were weighed and homogenized in DMEM containing 10% FBS and 1% antibiotics using 5 mm stainless steel beads (Qiagen) and the TissueLyser II (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... Cells were rinsed at rested at 37°C for a minimum of 1h before undergoing red-blood-cell lysis by 5-10 ml RBC lysis solution (Qiagen) for 20 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... The bead-bound gDNA was isothermally amplified for 3 hours at 30 °C then 10 minutes at 65 °C using a miniaturised (1/5 vols) Repli-g Single-Cell assay (Qiagen). The amplified gDNA was cleaned up with 0.8 × vols Ampure XP and 80 % ethanol ...
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bioRxiv - Microbiology 2023Quote: ... Metagenomic sequencing libraries were constructed from extracted DNA samples with 10 μL (1/5 volume) reactions using the QIAseq FX DNA Library Kit (QIAGEN). Each metagenomic sequencing library was sequenced using the Illumina NextSeq 2000 System 2 x 150 bp configuration.
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bioRxiv - Genomics 2022Quote: ... were annealed (10 μL each 100 μM) to 10 μL 5′-[Phos]-CTGTCTCTTATACACATCT-3’ oligonucleotide (100 μM) within 80 μL EB buffer (Qiagen), incubating 2 min at 95°C and cooled to room temperature (0.1°C/sec) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and 1x antibiotic-antimycotic by a TissueLyser II bead mill with 5-mm stainless steel beads (Qiagen). After brief centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... total RNA was extracted from the anterior half of the body from 10-12 5 dpf larvae per sample at using the Rneasy Mini Kit (Qiagen). cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Zoology 2024Quote: ... Multiplex PCR was performed in 10 µl reaction volume containing 5 µl 2x QIAGEN Multiplex PCR Master Mix (QIAGEN, Germany), 2 µl 5x Q-Solution (QIAGEN) ...
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bioRxiv - Neuroscience 2023Quote: ... A sample of the resulting homogenate (10-20 mg) was used to assess total cellular RNA using the RNeasy® Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... 20 nM biotin-NTA (QIAGEN) was charged with 50 nM Ni2+ and incubated on the slide for 10 min before rinsing away the unbound excess biotin-NTA and Ni2+ with PBS (this step was omitted for the labeled DNA samples) ...
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bioRxiv - Neuroscience 2022Quote: ... 20 μL proteinase K (QIAGEN) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... HGF (5’-CTCACACCCGCTGGGAGTAC-3’, 5’-TCCTTGACCTTGGATGCATTC-3’), STAT3 (5’-CTTTGAGACCGAGGTGTATCACC-3’, 5’-GGTCAGCATGTTGTACCACAGG-3’) and B-Actin Primer Set (Qiagen, QT00095431). After preparing master mixes samples were prepared in quadruplicate in a 96-well Reaction Microplates (Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... the DNA was amplified for 15 cycles and purified (QIAGEN). Three rounds of selection were performed (DNA was quantified by absorbance at 260 nm before each round of binding) ...
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bioRxiv - Biochemistry 2020Quote: ... 15 g of semi-dry Ni-NTA resin (Qiagen 30450) was weighed out into each of three conical tubes ...
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bioRxiv - Microbiology 2023Quote: ... using the BioSprint 15 DNA Plant Kit (QIAGEN, Hilden, Germany), all according to manufacturers’ instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 7 and 15 days and purified using RNeasy kit (Qiagen). 1 μg of RNA per condition was used to retrotranscribe into cDNA QuantiTect® Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... 16 µg of plasmid and 15 µL of Effectene (Qiagen). Cells were collected after 48 hours and prepared for the RNA-IP experiment ...
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bioRxiv - Developmental Biology 2024Quote: ... including a 15 min of DNase I (Qiagen; ref 79254) treatment.
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bioRxiv - Microbiology 2024Quote: ... extraction were performed using 15 ml gel-lock tubes (QIAGEN), followed by chloroform-isoamyl alcohol (Merck ...
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bioRxiv - Zoology 2024Quote: ... using 15 ul of Qiagen Multiplex PCR Mix (Qiagen, GER), 0.6 uM of forward and reverse primers and 1ul of DNA ...
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bioRxiv - Cell Biology 2024Quote: ... 15 uL of diluted RNase A (Qiagen 19101, 10mg/mL) was added to each sample and mixed thoroughly ...
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bioRxiv - Neuroscience 2021Quote: ... spun down (400 g × 10’ at 4 °C) and the pellet was resuspended in 600μ of Qiazol Lysis Reagent (Qiagen, Cat.No. 79306) and kept at −80 °C before sequencing.
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bioRxiv - Immunology 2021Quote: ... Samples were centrifuged at 14,000 g at 4°C for 10 mins and the supernatant collected for processing using a RNeasy mini-isolation kit (Qiagen Ltd, UK) according to the manufacturers’ protocol ...
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bioRxiv - Microbiology 2023Quote: ... were weighed and homogenized in 1-10 µL/mg DMEM with a sterile glass bead at 30 Hz for 4 minutes using a TissueLyser (Qiagen, Germantown, MD) automated homogenizer ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and RNA from patient tumor tissue biopsies and mouse tumors were extracted from 4 sections of 10 µm each from FFPE tumor blocks using the AllPrep® DNA/RNA FFPE kit (Qiagen #80234) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2021Quote: ... PCR amplifications were carried out in 10 μl reaction volumes containing 5 μl Type-it Microsatellite PCR Kit (Qiagen, Hilden, Germany), 0.5 μM of each primer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Multiplex amplification was carried out in 10 μl reaction volumes containing 5 μl of QIAGEN Multiplex PCR Buffer Mix (QIAGEN Inc.), 0.2 μM labeled forward primer (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 mL of the culture was immediately treated with a double volume (10 mL) of RNA protect bacteria reagent (Qiagen, Germany) for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... or 6 µM and harvested 24 h later by adding RLT lysis buffer (Qiagen). Similarly ...
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bioRxiv - Neuroscience 2020Quote: RNA was extracted from 24 hpf sfpq-/- embryos using the RNease Mini Kit (Qiagen). Reverse transcription was performed using the 3’ RACE System for Rapid Amplification of cDNA Ends (ThermoFisher) ...
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bioRxiv - Systems Biology 2021Quote: ... 24 and 48 hpi using the Qiagen RNAeasy plus extraction kit (Qiagen, Hilden, Germany). For quantifying SARS-CoV-2 genome abundance in mock samples ...
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bioRxiv - Evolutionary Biology 2020Quote: ... MinElute purification was carried out using the QIAvac 24 Plus vacuum manifold system (QIAGEN). Parallel non-template controls were included ...
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bioRxiv - Molecular Biology 2021Quote: ... 24 h later cells were transiently transfected with SuperFect Transfection Reagent (Qiagen Ref. 301305), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... gDNA extractions from 24 hpf eggs were performed using QIAamp DNA Mini Kit (Qiagen) following the instructions from the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... and 24 hours followed by RNA extraction and purification using the RNeasy kit (Qiagen). Samples were checked for purity and concentration using a nanodrop and Bioanalyser ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with hsa-miR-24-3p miRCURY LNA miRNA Power Inhibitor (Qiagen, Germantown ...
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bioRxiv - Microbiology 2020Quote: ... and 24 hours post followed by RNA isolation using an RNeasy mini Kit (Qiagen) per the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... and 24 h post treatment and RNA was isolated using RNeasy Mini kit (Qiagen). cDNA was synthesized using the SuperScript III First Strand Synthesis kit (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... The sample was subsequently homogenized for two cycles at 2000 rpm (PowerLyzer 24, Qiagen). The steel beads were removed ...
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bioRxiv - Neuroscience 2023Quote: DNAs from 24 hippocampi were purified using the DNeasy Blood and Tissue Kit (Qiagen). Only DNA samples with 260/280 ratios greater than 2.0 were processed for methylation analysis ...
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bioRxiv - Immunology 2024Quote: ... [31] datasets was performed using CLC genomics workbench software version 24 (QIAGEN, https://digitalinsights.qiagen.com) using the Quantify miRNA tool version 1.3 with sequence length set to 15 – 40 nt or 15 – 30 nt ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 17-20 days after fertilization (20 dpf) using RNeasy Mini Kits (Qiagen catalog #74104). For samples collected at 2 dpf ...
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bioRxiv - Developmental Biology 2022Quote: ... 25 mg tissue were mixed with 20 μl 20 mg/ml Proteinase K (Qiagen, 19131) and 180 μl buffer ATL (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL 5× One-step RT-PCR buffer (QIAGEN), 10 μL Triton-x100 (0.3% ...
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bioRxiv - Cell Biology 2022Quote: ... and oligo 5 (Q4) 5’-CACCGAGCTCTTCGCCGAGTA-3’ (Qiagen, SI03058272).
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bioRxiv - Microbiology 2020Quote: ... Cell lysates were centrifuged at 16,000 g for 5 min and supernatant was treated with 4 µL of RNase A (100 mg/mL) (Qiagen Singapore Pte. Ltd, Cat. No. 19101), gently mixed by flicking of tube and incubated at room temperature for 2 min ...
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bioRxiv - Genomics 2022Quote: An aliquot of 150 μL of plasma per sample was thawed on ice and centrifuged at 3000 × g for 5 min at 4 °C and smallRNA was extracted using miRNeasy Serum/Plasma Kits (Qiagen, Cat. No. 217184, Milano, Italy) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA from 4-8 microdissected 10 μm FFPE sections were extracted using the QIAamp DNA FFPE Tissue kit (Qiagen, cat. no. 56404) according to manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... exposed to 0.4 nM and 4 nM of β-estradiol for 10 days) Vagina Chips using QIAzol lysis reagent (Qiagen, cat. no. 79306). Complimentary DNA was synthesized using a SuperScript VILO MasterMix (Invitrogen ...