Labshake search
Citations for Qiagen :
101 - 150 of 2805 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... miR-24-3p LNA inhibitor treatment: Cells were transfected with hsa-miR-24-3p miRCURY LNA miRNA Power Inhibitor (Qiagen, Germantown ...
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bioRxiv - Cancer Biology 2024Quote: ... siRNAs were added 24 hours after seeding at the final concentration of 25 nM with 12 to 24 μL HiPerFect (Qiagen). On day 5 after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μL of 10 μM double-stranded mature miRNA mimic (Dharmacon) or 20 μM LNA (Qiagen) was mixed with 3 μL lipofectamine RNAiMAX in 100 μL Opti-MEM reduced serum media and added to cells following a 15-minute incubation ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA (10 to 20 ng) was retrotranscribed by using the QuantiTect Reverse Transcription Kit (Qiagen). Quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from approximately 10–20 seedlings using the RNeasy Plant Mini Kit (QIAGEN) followed by DNase I treatment ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The tissue was ground to a fine powder by adding 10-15 zirconia beads (1 mm diameter) and using a TissueLyser (Qiagen) for sunflower ligules ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 % (v/v) glycerol) for 30 s at a frequency of 15 s-1 using a TissueLyser II (QIAGEN). As the tissue homogenates were to be used for both native and SDS PAGE ...
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bioRxiv - Immunology 2024Quote: Biopsies (n= 10 from non-IBD controls, n= 29 biopsies from 15 UC patients) were placed in RNA later (Qiagen) and stored at −80°C until further analysis ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was isolated from nodules 21 or 22 days after inoculation using an RNeasy kit (Qiagen). Five replicates for each construct were done ...
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bioRxiv - Immunology 2024Quote: ... RNA-seq results were further analyzed using Ingenuity Pathway Analysis (IPA, QIAGEN, version 01-22-01) for Upstream Regulator Analysis of differentiation expression genes identified in this study ...
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bioRxiv - Cancer Biology 2023Quote: ... according to the manufacturer’s instructions and eluted in 22 µL EB buffer (Qiagen, cat. no. 19086). DNA concentrations were measured using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... using a vacuum manifold (QIAvac 24 plus, Qiagen) and size selection was performed with SPRI Beads (Beckmann Coulter ...
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bioRxiv - Genomics 2021Quote: ... using a vacuum manifold (QIAvac 24 plus, Qiagen) and size selection was performed with SPRISelect reagent (Beckmann Coulter ...
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bioRxiv - Neuroscience 2020Quote: ... using a vacuum manifold (QIAvac 24 plus, Qiagen) and size selection was performed with SPRI Beads (Beckmann Coulter ...
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bioRxiv - Neuroscience 2020Quote: ... using a vacuum manifold (QIAvac 24 plus, Qiagen) and size selection was performed with SPRI Beads (Beckmann Coulter ...
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bioRxiv - Genetics 2021Quote: ... using a vacuum manifold (QIAvac 24 plus, Qiagen) and size selection was performed with SPRI Beads (Beckmann Coulter ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... containing protease inhibitor cocktail using PowerLyser 24 (Qiagen) and mixed gently at 4 °C for one hour ...
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bioRxiv - Neuroscience 2023Quote: ... for 24 h using an RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining lysate was then transferred to 15 ml tubes containing 5 ml lysis buffer and 150 μl Ni-NTA agarose beads (Qiagen, Venlo, Netherlands) and incubated for 4 h at room temperature under constant mild agitation ...
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bioRxiv - Microbiology 2021Quote: ... 20 mM imidazole was added to the supernatant and incubated with 5 mL of Ni-NTA resin (Qiagen) with a stirring magnet at 4°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared using a JA-20 rotor (18,000 rpm for 30 min at 4°C) and incubated with Ni-NTA superflow resin (Qiagen) for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer A supplemented with 200 mM imidazole in gravity flow ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting with buffer A supplemented with 250 mM imidazole in gravity flow ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 mM GDPNP-Mg2+) were obtained at 4°C using 0.2 M Lithium nitrate and 20% PEG3350 (PEG Suite I, Qiagen) as precipitating agent ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B supplemented with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... cleared by centrifugation (20 min, 20,000 × g, 4°C) and the clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer C supplemented with 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA agarose resin (QIAGEN) eluted with buffer A supplemented with 0.5 mM TCEP and 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and cleared lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Genomics 2022Quote: ... samples were incubated an additional 30 minutes with 20 µL Proteinase K and 4 µL RNase A (Qiagen 19101) and subsequently eluted for DNA using DNeasy Blood and Tissue kit ...
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bioRxiv - Genomics 2023Quote: ... samples were incubated an additional 30 minutes with 20 uL Proteinase K and 4 uL RNase A (Qiagen 19101) and subsequently eluted for DNA using DNeasy Blood and Tissue kit.
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Genetic underpinnings of predicted changes in cardiovascular function using self supervised learningbioRxiv - Systems Biology 2024Quote: RNA was extracted from frozen PBMCs with an All-prep DNA/RNA 96 (4) kit (QIAGEN, Cat# 20-80311) on a Tecan Evo 200 automated platform ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from untreated cells (24 h) and IFNγ-treated cells (6 or 24 h) were extracted with RNeasy plus mini kit (Qiagen, 74134) according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was incubated for 2h at 4°C with 5 ml of Ni-NTA agarose (Qiagen) pre-equilibrated in wash buffer 1 WB1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were digested for 60 minutes with proteinase K (10 µl) followed by bead-beating for 40 minutes (Vortex-Genie 2 with vortex adapter, Qiagen, Cat: #13000-V1-24) at maximal speed ...
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bioRxiv - Immunology 2020Quote: ... Mechanical disruption of microbial cells was done using TissueLyser II (QIAGEN, 30 shakes/sec., 10-20 min.) and the samples were centrifuged (10,000 g ...
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bioRxiv - Genomics 2021Quote: ... in a 20 μL system with a mixture of 10 μL 2×SYBR Premix ExTaq (Qiagen, Germany), 2.0 μL diluted cDNA or double distilled water as a negative control ...
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bioRxiv - Immunology 2022Quote: ... Genomic DNA was extracted from sort-purified cells from 22 patients by QiaAmp DNA micro kit (Qiagen). For reliable identification of expanded clones ...
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bioRxiv - Microbiology 2020Quote: ... DNA was isolated as previously described [22] using the QIAamp DNA Mini Kit (Qiagen Inc., Chatsworth, CA). RNA extraction was performed following Trizol manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and 20 ng cDNA were subjected to the Rotor-Gene Q 5-Plex HRM real-time PCR system (Qiagen), using the default program ...
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bioRxiv - Immunology 2022Quote: ... Enriched cells were surface stained and 5-20 ×103 intratumoral cDC1s were sorted directly in RLT lysis buffer (Qiagen) containing 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA (2000 ng) was transcribed into cDNA in a volume of 20 μl using 4 U Omniscript reverse transcriptase (Qiagen). To determine the relative abundance of the mRNAs of interest ...
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bioRxiv - Synthetic Biology 2020Quote: ... Lysates were clarified by centrifugation at 24,000gat 4 °C for 20 min and passed through 2 ml of Ni-NTA nickel resin (Qiagen, 30250) pre-equilibrated with wash buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were clarified by centrifugation at 24,000g at 4°C for 20 min and passed through 2 mL of Ni-NTA nickel resin (Qiagen, 30250) pre-equilibrated with wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining supernatant was centrifuged again at 5,000 x g for 20 minutes at 4°C and equilibrated for 1 hour in 3 mL of Ni-NTA resin (Qiagen) that was pre-equilibrated in the extraction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... was reverse transcribed at 37 °C for 2 h in a 20 μL reaction volume containing 4 U of Omniscript reverse transcriptase (Qiagen), 0.5 mM each dNTP ...
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bioRxiv - Microbiology 2021Quote: ... A bench top PowerLyzer 24 Homogenizer (QIAGEN, Hilden, Germany) was used for homogenizing the samples at 2000 rpm for 30 s ...
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bioRxiv - Bioengineering 2023Quote: ... then placed in a tissue homogenizer (Powerlyzer 24, Qiagen) using the following settings ...
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bioRxiv - Developmental Biology 2023Quote: ... along with a vacuum manifold (QIAvac 24 plus, Qiagen) was used for library purification ...
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bioRxiv - Bioengineering 2023Quote: ... then placed in a tissue homogenizer (Powerlyzer 24, Qiagen) using the following settings ...
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bioRxiv - Immunology 2023Quote: ... samples were homogenized in PowerLyzer 24 Homogenizer (Qiagen, #13155) two times at 4m/s for 10 seconds ...