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Citations for Qiagen :
251 - 300 of 2805 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: For qBiCo-v1 the PCR reaction was optimized in a final volume of 20 µl containing: 10 µl of 2x EpiTect® MethyLight qPCR reagent (Qiagen), 2 μl of 25 mM MgCl2 (Applied Biosystems ...
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bioRxiv - Bioengineering 2023Quote: Sludge samples were collected at 22 time points during the experiment from MBR for DNA extraction using the DNeasy PowerSoil Pro Kit (QIAGEN, Germany). Sequencing of DNA followed the methods described in our previous study 6 ...
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bioRxiv - Molecular Biology 2020Quote: ... approximately half of the infected tissue was cut into small pieces and DNA was extracted using a BioSprint 15 instrument and BioSprint 15 DNA plant kit (Qiagen, Australia) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR analysis was performed using 15 ng of cDNA to a final volume of 15 μL reaction with Rotor-Gene SYBR® Green PCR Kit (Qiagen), in a Rotor Gene-Q (R ...
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bioRxiv - Molecular Biology 2020Quote: Cells were harvested from 24-well plates in 350μL RLT Plus (Qiagen) supplemented with 1% beta-mercaptoethanol after an 8-hour induction with 10nM E2 or DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... according to the manufacturers’ protocol 24 hours before experiments whereas HiPerfect (Qiagen) reagent was used for transfecting 10 nM siRNA per target 72 hours before experiments ...
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bioRxiv - Plant Biology 2020Quote: ... Initial hits were further optimized in 24-well sitting-drop plates (Qiagen) using as reservoir solution 18-22 % PEG 3350 ...
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bioRxiv - Genomics 2021Quote: ... Sample homogenisation and lysis were performed using PowerLyzer 24 (Qiagen, Hilden, Germany) set at 2,000 rpm for 2 × 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... the sequencing reads were first imported into CLC Genomics Workbench 24 (Qiagen), merged ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µl Polyfect (Qiagen) and 1 µg plasmid were incubated for 5 minutes and the transfection mix was dropped to cover all cells without medium ...
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bioRxiv - Genetics 2020Quote: ... DNA binding plate was incubated with 600 μL of liquid for 4 minutes at RT and centrifuged at 1,300 g for 5 minutes sequentially with PB buffer (Qiagen Cat. No. 19066), followed by PE buffer (Qiagen Cat ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was cleared by centrifugation at 18000 rpm at 4°C and the supernatant was loaded onto 5 mL Ni–NTA resin (Qiagen, Milan, Italy) equilibrated with binding buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells attached to the collagen matrix were fixed with 4% PFA for 10 minutes at room temperature for IF analysis or lysed with RLT buffer (Qiagen) for RNA isolation and qRT-PCR analysis as described above.
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bioRxiv - Plant Biology 2021Quote: ... We extracted total RNA from 10 leaves that were shorter than 500 µm and from 4–6 mature leaves using the RNeasy Micro Kit (Qiagen) and the RNeasy Plant Mini Kit (Qiagen) ...
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bioRxiv - Biochemistry 2020Quote: ... 80 µL of the mixture was then incubated for 45-60 minutes at room temperature or 4°C with 10 µL NiNTA resin (Qiagen) prewashed with His-SUMO Lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified extract was supplemented with 10 mM imidazole and rotated for 2 hours at 4 °C with 1 ml of Ni-NTA beads (Qiagen). Bound protein was eluted with 10 CV of buffer L / 200 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted from the supernatant (12,000 x g for 10 min at 4°C) of centrifuged tissue homogenate using an RNeasy Mini Kit (Qiagen, USA). The extracted total RNA was used for single-strand cDNA synthesis ...
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bioRxiv - Neuroscience 2023Quote: ... freshly sorted cells were pelleted at 500 g for 10 minutes at 4° and then resuspended in RLT Plus buffer (Qiagen). Cells were vortexed and frozen for at least one day at −80° before being thawed on ice and processed for RNA using an RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining cells were pelleted at 1000 x g for 10 minutes at 4°C and lysed in 350 µL of Buffer RLT (Qiagen) supplemented with 3.5 µL of 2-β mercaptoethanol (#444203 ...
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bioRxiv - Microbiology 2023Quote: ... Pellets were washed in 2 mL cold 10 mM NaCl + 4 mL cold RNAprotect Bacteria Reagent (Qiagen Cat. No. 76506) and repelletted at 4,255 x g ...
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bioRxiv - Developmental Biology 2023Quote: ... Then samples and inputs were incubated for 4 hr at 37°C with 1.5 µl of 10 mg/ml RNase A (Qiagen, 1007885) and 15 µl 10% sodium dodecyl sulfate and 3.5 µl 20 mg/ml Proteinase K (Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: Ten aphids (n = 10 × 4) were ground to powder with a liquid nitrogen-cooled micropestle after which the RNeasy kit (Qiagen) was used for RNA extraction with on-column DNA digestion (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: RNA from 5-10 million NK cells treated overnight with indicated conditions was extracted using Rneasy Plus kit (Qiagen). Samples were prepared for Illumina sequencing following the manufacturer’s protocol using the TruSeq® stranded total RNA with rRNA depletion using Ribo Zero TM Human/Mouse/Rat ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was loaded onto a 10 mL Ni-NTA Superflow cartridge (connected two 5 mL cartridges) (30761, Qiagen). The cartridge was washed with 100 mL R buffer containing 200 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated in lysis buffer (DPBS with 0.5% Triton X-100, 10 mM MgCl2, 5 mM CaCl2, 100 µg/mL RNAse A (Qiagen)) overnight at 37°C in a water bath to allow capsid maturation ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from 5-10×106 cells using the Gentra Puregene Cell Kit (Qiagen, cat no. 158046) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then incubated in lysis buffer (DPBS with 0.5% Triton X-100, 10 mM MgCl2, 5 mM CaCl2, 100 µg/mL RNAse A (Qiagen)) overnight at 37°C in a water bath to allow capsid maturation ...
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bioRxiv - Bioengineering 2022Quote: Decellularization for porcine AECMs met the stringent criteria for decellularized ECM.15 Residual dsDNA content was quantified from 15 mg of powdered ECM using the QIAamp DNA Mini Kit (QIAgen, Germantown, MD) followed by Qubit 2.0 (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Lyophilized material was pulverized in a 2ml screw top microcentrifuge tube with a glass bead for 10 minutes at a frequency of 20/s in a TissueLyser (Retsch ballmill, Qiagen, Venlo, Netherlands). The material was AIR prepped and destarched using protocols from Barnes and Anderson (Barnes and Anderson ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was isolated from soleus muscle (10 µg) and apex of ventricle (20 µg) using RNeasy fibrous tissue mini kit (QIAGEN, Hilden, Germany) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: DNA was isolated from 10-20 8-μm frozen tissue sections of biopsies from humanized mice using the PureGene DNA isolation kit (Qiagen, Hilden, Germany). PCR was performed with six framework region 1 subgroup specific primers and a JH primer mix (3’ JH mix ...
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bioRxiv - Molecular Biology 2023Quote: ... 600 µl of lysis buffer (TES (0.1 M TRIS, 10 mM EDTA, 2% sodium dodecyl sulphate; pH 8) and Proteinase K (Qiagen, 20 mg/ml) in a 20:1 ratio ...
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bioRxiv - Microbiology 2020Quote: ... that included a 15 min DNAse treatment (Qiagen, 79254) treatment at RT ...
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bioRxiv - Immunology 2020Quote: ... with a 15-minute on-column DNase digestion (Qiagen) to remove genomic DNA ...
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bioRxiv - Immunology 2020Quote: ... and 15% Glycerol from the JCSG Core II (Qiagen) screen ...
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bioRxiv - Immunology 2021Quote: ... DNAse treatment (15 min at RT; Qiagen, Hilden, Germany) was only conducted in samples used for RNASeq but not in samples used for qPCR analysis ...
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bioRxiv - Microbiology 2023Quote: ... EasyXtal 15-well Tool crystal plates (Qiagen, Manchester, UK) were set up manually for HAdV-D15 ...
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bioRxiv - Genomics 2024Quote: ... 1% SDS with 15 uL Puregene Proteinase K (Qiagen)) at 55°C for 2 hours with inversion every 30 minutes ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 12-d-old seedlings grown at 22°C in LD conditions at ZT13 with the RNeasy® Plant Mini Kit (QIAGEN, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: * RNA extraction: Total RNA was isolated from frozen HT-22 cells or brain tissue with the RNeasy Mini Kit (Qiagen, Courtaboeuf, France). Total RNA from living HT-22 cells were isolated using Trizol (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Ligated cDNA samples were cleaned and eluted in 24 μl Elution Buffer (Qiagen) using Oligo Clean & Concentrator (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... was performed approximately 24 h after cell plating using Effectene Transfection Reagent (Qiagen) with 1 µg siRNA in 742 µL transfection mixture ...
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bioRxiv - Physiology 2024Quote: ... The reaction was run in the QIAcuity Nanoplate 26k 24-well plates (QIAGEN). The 40 µl reaction mix consisted of 10 µl of 4x QIAcuity Probe PCR Kit (QIAGEN) ...
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bioRxiv - Immunology 2023Quote: RNA was isolated 24 hours after transfection of HEK293T cells (Qiagen, Cat# 74004). cDNA was synthesized using Verso cDNA synthesis kit (Cat# Ab1453A ...
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bioRxiv - Bioengineering 2023Quote: ... The dPCR reactions were performed in a 26K 24-well Nanoplate (Qiagen, 250001) on a 5-plex QIAcuity One digital PCR instrument (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... and 24-hours of growth using Qiagen’s RNeasy Mini Spin Kit (Qiagen #74104) and subjected to DNAse digestion by combining 5 µg RNA with 1 µL TURBO DNAse enzyme ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted at 24 hpi using RNeasy Mini kit (Qiagen, #74106) following manufacturer’s specifications ...
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bioRxiv - Biochemistry 2021Quote: ... Medium was stirred for 1 hour at 4°C in the presence of 5 ml Ni-NTA Agarose beads (Qiagen, Venlo, The Netherlands) washed with 25 mM Hepes-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then incubated for ~ 10 min with 4 μg/ml of Penta•His biotin conjugate antibody (34440, Qiagen, UK) in reaction buffer [40 mM HEPES buffer (pH 7.3 ...
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bioRxiv - Cancer Biology 2020Quote: DNA and RNA were extracted from the cervical cancer tissues (5-10 mg) using the AllPrep DNA/RNA Micro Kit (QIAGEN) as described by the manufacturer ...