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Citations for Qiagen :
2851 - 2900 of 3857 citations for 6 Chloro 4 hydroxy 2 methyl 2H thieno 2 3 e 1 2 thiazine 3 carboxylic acid methyl ester 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Routine qPCR for miR detection and validation (see Supplementary Table 1 for miR sequences) was performed with the miScript PCR control set (catalog number 218380; Qiagen, Germany). The miScript™ miRNA PCR Array Human Serum & Plasma 384HC (Cat No ...
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bioRxiv - Immunology 2022Quote: ... The middle and inferior right lobes were weighted and homogenized with PBS (1:5 w/v) using a 5 mm stainless steel bead (Qiagen, USA) and a TissueLyser LT (Qiagen ...
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bioRxiv - Pathology 2022Quote: ... tissue samples were homogenized in 1 ml of phosphate buffered saline with a metal bead using a TissueLyzer II (Qiagen GmbH). Viral nucleic acids were extracted from blood and homogenized spleen ...
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bioRxiv - Systems Biology 2022Quote: ... the samples were thawed and physically disrupted using a beadmill (1 min max speed) using a TissueLyser LT (Qiagen, Hilden, DE) and then extracted with phenol-chloroform including an extra chloroform step and several RNA washing steps ...
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bioRxiv - Microbiology 2023Quote: ... we used low-binding plastics and added 1 µl of 0.1 ng/µL tRNA carrier (i.e. spike water, Qiagen, Cat. No. 1068337) in each tube with stock eluted DNA ...
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bioRxiv - Neuroscience 2022Quote: ... 30µL of DNase and RDD buffer (1:7 ratio) containing solution from the RNase-Free DNase Set (Qiagen, Catalog No. 79254) was incubated on the columns at room temperature for 15 minutes before a second and third round of RNA was performed as well as a final spin to ensure the column was dry ...
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bioRxiv - Cell Biology 2022Quote: ... were digested with DpnI for 1 hour at 37°C and subsequently purified with the QIAquick Gel Extraction Kit (Qiagen #28704). They were ligated with homemade Gibson Master Mix (Table S1 ...
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bioRxiv - Biophysics 2024Quote: ... Cleavage products were separated on a 1% agarose gel and the linearised plasmid was purified with a QIAquick Gel Extraction Kit (Qiagen, #28706). Complementary synthetic DNA oligos coding for miR-34a target sites (Table S1) ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were initially preserved with 1 mL of activated sludge (i.e., suspended phase) in 9 mL of LifeGuard Soil Preservation (Qiagen, Hilden Germany). This solution was thawed and centrifuged (16,000 g for 1-minute ...
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bioRxiv - Microbiology 2024Quote: ... samples were thawed and homogenized at a frequency of 26/s for 1 minute in a TissueLyser II (Qiagen, Germantown, MD) and centrifuged at 16,500xg for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from the cortical brain and cochlea samples from 1-month-old mice using RNA extraction kit and Qiazol Reagent (RNeasy mini kit; Qiagen, Germany), and DNase treatment was performed (RNase-free ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The concentration of the resulting His-tagged ß-CATENIN-1 fragments was quantified via quantitative Western blotting with a Penta-His antibody (Qiagen) and a recombinant His-tagged protein as external standard.
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bioRxiv - Cell Biology 2023Quote: ... total RNA was isolated from C2C12 myoblasts transfected with GFP-empty vector or GFP-Δ(1-240)JPh1 using the RNeasy-plus mini kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... washed in ice-cold PBS and resuspended in 350μl of Buffer RLT Plus supplemented with 1% (v/v) β-mercaptoethanol from the RNeasy Plus Mini Kit (Qiagen, 74134). RNA was extracted using the same kit according to manufacturer’s instructions and quality was assessed using the Agilent 2200 TapeStation System (Agilent ...
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bioRxiv - Biochemistry 2022Quote: ... Memorial Sloan Kettering Cancer Center) at a dilution of 1:5,000) and His (mouse anti-penta-His antibody (Qiagen, cat. #34660) at a dilution of 1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... a small amount of tissue (>25 mg) was added to 1 mL of TRIzol with a single 5mm stainless steel bead (Qiagen, Germany) and homogenised using a TissueLyser II (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: ... samples were suspended in RNeasy kit Lysis Buffer and β-mercaptoethanol (1:100 v/v) and homogenized using QIAshredder columns (Qiagen). Samples loaded on spin columns were treated with DNase (Qiagen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following an incubation at 55°C for 1 hr in 450 µl of lysis buffer with proteinase K (QIAGEN, Hilden, Germany). Each colony was genotyped using twelve microsatellite loci69 ...
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bioRxiv - Zoology 2023Quote: ... The mixtures were incubated at 56 °C for 1 h before DNA was extracted using an QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting amplicon was cut from a 1 % agarose gel and purified using the QIAGEN PCR purification kit (QIAGEN, Hilden, Germany). Afterwards ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed using RLT buffer mastermix (10 uL beta-mercaptoethanol : 1 mL RLT) and cells were lysed with RNAeasy micro kit (Qiagen, 74004). RNA quality and quantity was validated using a nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... The digested vectors were run on 1% agarose gel and the target band was cut and harvested using QIAquick Gel Extraction Kit (QIAGEN 12362). The gel extraction products were further purified with QIAGEN MinElute PCR purification kit (QIAGEN 28004) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated from BCBL-1-RTA at the indicated times following reactivation using the Dneasy blood and tissue kit following manufacturer guidelines (Qiagen 69581). 20ng of total DNA was used for qPCR using primers for the KSHV gene ORF57 ...
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bioRxiv - Molecular Biology 2024Quote: ... NEM was subsequently aspirated and the tissue homogenized for 1 min at 30 Hz using a TissueLyser II bead mill (QIAGEN, USA) in ice-cold homogenization buffer [10% glycerol ...
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bioRxiv - Bioengineering 2024Quote: ... IVD cells that were attached to the well were washed with 1% dPBS and lysed using RLT buffer (Qiagen, Hilden, Germany) and 1% mercaptoethanol (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... The digest was run on an 1% Agarose gel (containing ethidium bromide) and the pLV6 backbone was extracted with the QIAquick Gel extraction kit (Qiagen 28704) according to the manufacturer’s manual.
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bioRxiv - Cell Biology 2024Quote: Cell pellets or lung lobes from individual mice were snap frozen in 350µl of 1:100 β-mercaptoethanol in the RLT Lysis Buffer from the RNAeasy mini plus Kit (Qiagen, 74134). Cell samples were thawed and vortexed while lung samples were homogenized for total RNA extraction intended for qPCR or bulk RNA-seq ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µg of RNA was used as input for cDNA synthesis using the RNA QuantiTect transcription kit (Qiagen, Cat # 205311). Approximately 100 ng of cDNA was used as a template for each qRT-PCR reaction using the PowerTrack SYBR Green supermix (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 PPKS/M and 4 PPKS/Y) using the AllPrep DNA/RNA Mini Kit (Qiagen). Indexed libraries were generates using the KAPA mRNA HyperPrep kit and sequenced on a NextSeq500 sequencer (NextSeq 500/550 High Output Kit v2.5 ...
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bioRxiv - Cell Biology 2020Quote: ... For SAS-6 knock down experiments siNegative control (siNegative, Qiagen, 1027310) and siSAS-6 (siSAS6 on-TARGET smart pool ...
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bioRxiv - Plant Biology 2022Quote: ... 6 or 24 h using an RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: RNA interference against MUS81 was performed using FlexiTube HsMUS81 6 (Qiagen) at final concentration of 10 nM using Lullaby 48 h before to perform experiments ...
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bioRxiv - Developmental Biology 2024Quote: ... 6×105 cells were resuspended in 600 µl RLT buffer (Qiagen) and snap frozen on dry ice for later processing ...
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bioRxiv - Microbiology 2020Quote: Nucleic acids were extracted using the AllPrep DNA/RNA Micro kit (Qiagen) for simultaneous purification of minute amounts of DNA and RNA from the same sample without making use of the carrier RNA ...
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bioRxiv - Microbiology 2019Quote: Nucleic acid extraction was performed with Advanced XL EZ1 (Qiagen, Hilden, Germany). The digestion step using proteinase K was performed for 10 minutes at 56 °C and 10 minutes at 95 °C followed by purification with EZ1 Tissue card according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Total nucleic acid was extracted with QIAcube HT system (Qiagen, Valencia, Calif.), according to the manufacturer’s instructions for tissue or insects ...
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bioRxiv - Cell Biology 2020Quote: ... the resulting supernatant was incubated with nickel-nitrilotriacetic acid agarose beads (QIAGEN) pre-equilibrated with the washing buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysate was incubated with Ni-nitrilotriacetic acid (NTA) resin from Qiagen for 1hr at RT ...
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bioRxiv - Zoology 2021Quote: Nucleic acids were extracted using a QIAamp MinElute Virus Spin Kit (QIAGEN) and used to construct the sequencing libraries ...
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bioRxiv - Microbiology 2020Quote: ... then the nucleic acid was purified using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Viral ribonucleic acid (RNA) was extracted by viral RNA kit (Qiagen, Germany), reversed by reverse transcription kit (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... Ni(II)-nitrilotriacetic acid agarose (Ni-NTA) resin was purchased from Qiagen. L-Allylglycine was purchased from Cayman Chemical Company ...
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bioRxiv - Molecular Biology 2022Quote: ... the His-SUMO conjugates were enriched using nickel-nitrilotriacetic acid beads (Qiagen). Upon multiple washing steps ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 µL of dry nickel-nitrilotriacetic acid-agarose (Ni-NTA) beads (QIAGEN), were equilibrated with Guanidinium buffer supplemented with 5 mM β-mercaptoethanol and 50 mM Imidazole pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by purification using nickel-nitrilotriacetic acid (Ni-NTA) agarose resin (QIAGEN). The recombinant protein was desalted with PD-10 column (SephadexTM G-25M ...
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bioRxiv - Genomics 2019Quote: ... Plasma DNA was extracted using the QiaAmp Circulating Nucleic Acids kit (Qiagen). DNA was extracted from FFPE ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acids were extracted with the QIAamp Viral RNA mini kit (QIAGEN) to be further randomly amplified using a modified Whole Transcriptome Amplification 2 (WTA2 ...
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bioRxiv - Microbiology 2021Quote: ... the supernatant was extracted with a nickel-nitrilotriacetic acid-agarose suspension (Qiagen) or a Pierce GST Spin Purification Kit (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 μL of dry nickel-nitrilotriacetic acid-agarose (Ni-NTA) beads (QIAGEN), were equilibrated with Guanidinium buffer supplemented with 5 mM β-mercaptoethanol and 50 mM Imidazole pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... the supernatant was incubated with Ni-nitrilotriacetic acid (NTA) agarose resin (Qiagen) for 1 hour at 4 °C ...