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Citations for Qiagen :
2751 - 2800 of 3857 citations for 6 Chloro 4 hydroxy 2 methyl 2H thieno 2 3 e 1 2 thiazine 3 carboxylic acid methyl ester 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Worms were snap frozen in liquid nitrogen before being ground into a fine powder for 1 min by a stainless-steel bead in a TissueLyser LT (Qiagen) operating at 50 oscillations per second ...
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bioRxiv - Microbiology 2023Quote: ... Metagenomic sequencing libraries were constructed from extracted DNA samples with 10 μL (1/5 volume) reactions using the QIAseq FX DNA Library Kit (QIAGEN). Each metagenomic sequencing library was sequenced using the Illumina NextSeq 2000 System 2 x 150 bp configuration.
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bioRxiv - Microbiology 2023Quote: ... DNA extraction aimed at producing fragments adequate for long-read sequencing and followed either of two methods: 1) DNeasy PowerSoil Kit (Qiagen) without bead-beating and vortexing steps ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated at 37 °C with agitation for six hours after which time 1 mL cell pellets were treated with RNAprotect (Qiagen). Cell pellets were washed in 0.5 mL PBS pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... about 100 mg of tissue samples were homogenised in 1 mL of TRI reagent at room temperature using a tissue lyser (Qiagen). The homogenised samples were transferred to fresh microcentrifuge tubes for RNA isolation ...
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bioRxiv - Biochemistry 2023Quote: ... HBEC5i cells with treated with either non-specific siRNA or Flvcr2 siRNA were harvested and kept in 1 mL TRI reagent (Qiagen).
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bioRxiv - Bioengineering 2023Quote: ... Lysate supplemented with 20 mM imidazole was cleared by centrifugation at 15000 g at 4 °C for 30 minutes and the supernatant was then loaded onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) pre-washed with 5 column volumes of 1x TBS (pH 7.3) ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were pelleted at 15000 g at 4 °C for 30 minutes and the supernatant was supplemented with 20 mM of imidazole before loading onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) pre-washed with 5 column volumes of 1x TBS (pH 7.3) ...
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bioRxiv - Microbiology 2023Quote: ... Illumina sequencing-compatible Combinatorial Dual Index (CDI) adapters were ligated onto pooled amplicons using the QIAseq 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation and sequenced on Illumina MiSeq platform to generate 2×250bp reads ...
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times with ice-cold PBS and lysed at room temperature with 1 ml/well QIAzol lysis reagent (QIAGEN). Lysates were incubated at room temperature for 5 min and homogenised by pipetting up and down ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatants were then supplemented with 20 mM imidazole and then loaded onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) that were pre-washed with 5 column volumes of 1x PBS ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA libraries were created using genomic DNA extracted from 2-week old Col-0 seedlings grown on ½ Murashige and Skoog media supplemented with 1% sucrose with DNeasy Plant Mini kit (Qiagen). Plasmids containing the proteins of interest in the pIX-HALO backbone were ordered from ABRC ...
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bioRxiv - Microbiology 2023Quote: Total genomic DNA was extracted from HSV-1 ANG and ANG path virions using the QiaAmp Mini DNA Kit (Qiagen). Full-length nucleotide sequences of UL44 ...
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bioRxiv - Microbiology 2023Quote: ... Illumina sequencing-compatible Unique Dual Index (UDI) adapters were ligated onto the pools using the QIAseq 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation and sequenced on the Illumina MiSeq platform to generate 2×250bp reads.
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bioRxiv - Molecular Biology 2023Quote: cDNA was produced from 1 μg of total RNA (final cDNA concentration 5 ng/ml) using QuantiTech Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... we added 1 µl of the spike-in mix provided in the QIAGEN RNA Spike-In Kit (Cat. No.: 339390, QIAGEN) to the tube ...
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bioRxiv - Immunology 2024Quote: ... and homogenized via sonication at 20 kHz for 1 min before RNA isolation was performed using a RNeasy Mini Kit (Qiagen). For qPCR ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was subjected to immobilized metal affinity chromatography using a 1 mL Ni-NTA agarose column (Qiagen, 30210) equilibrated with binding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The reactions were incubated for 15 min at 60°C and then terminated by adding 1 μL 5 M NaOH to degrade RNA and heating at 95 °C for 5 min followed by neutralization with 1 μL 5 M HCl and one round of MinElute column clean-up (Qiagen). The R1R DNA adapter was adenylated by using a 5’ DNA Adenylation kit (New England Biolabs ...
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bioRxiv - Physiology 2024Quote: ... The digest was run on an 1% Agarose gel (TAE buffer, 0.25 µg/ml EtBr) and the the QIAquick Gel extraction kit (Qiagen, 28704) was used to extract the pLV6 backbone according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The solubilized supernatant was collected by another round of ultracentrifugation (200,000 × g, 1 h) and applied to a Strep-Tactin resin column (Qiagen, USA) equilibrated with buffer A containing 0.025% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was treated with 40U Dpn I for 1 hour and was purified by QIAquick PCR Purification Kit (QIAGEN). The purified DNA was quantified by Qubit dsDNA BR kit (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... The reaction mixture was incubated at 37°C for 1 hour followed by purification using QIAquick PCR Purification Kit (QIAGEN). The purified DNA was quantified by Qubit dsDNA BR kit (Invitrogen).
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bioRxiv - Developmental Biology 2024Quote: ... 5 µl of each PCR product was run in a 1% agarose gel and the remaining 35 µls were purified using the QIAquick PCR Purification Kit (Qiagen) before being sent for sequencing (sequencing primers ...
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bioRxiv - Microbiology 2024Quote: ... A 1 μg of total RNA of each sample was reverse-transcribed to cDNA using RT2 first strand kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... semimature SP thymocytes were isolated on BD FACSAria II and BD FACSAria Fusion cell sorters directly into RLT + 1% beta-mercaptoethanol (BME) buffer (Qiagen). Libraries were generated by the Emory Integrated Genomics Core (EIGC ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were separated on a 1% agarose gel and bands were excised using a QIAquick gel extraction kit (Qiagen). The purified PCR bands were cloned using the NEB PCR cloning kit following manufacturer’s instructions and transformed colonies were screened using colony PCR ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5 mm (0.3 g) Zirconia beads and 1 mL of InhibitEx buffer from the QIAmp Fast DNA Stool Mini Kit (QIAGEN #51604) in a 2 mL tube ...
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bioRxiv - Microbiology 2024Quote: Infected whole eyes were homogenized in 1 mL of sterile PBS using a Retsch Mixer Mill MM300 (Qiagen, Valencia, CA) at 33 Hz for 4 min ...
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bioRxiv - Immunology 2024Quote: Genomic DNA samples were prepared from heparinized peripheral blood or 1×106 Jurkat cells following the instructions of the QIAamp DNA Blood Mini kit (#51104; QIAGEN). Primers were designed with the help of Oligo Primer Analysis Software version 7 (Molecular Biology Insights) ...
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bioRxiv - Genomics 2022Quote: ... 400 nL of protease mix (6 μg protease (Qiagen, 19155), 6.25x NEBuffer 4 (NEB ...
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bioRxiv - Microbiology 2021Quote: ... 6 and 9 employing the RNeasy plant mini kit (Qiagen). RNA was quantified using Nanodrop (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... For MUS81 interference it was used FlexiTube HsMUS81 6 (Qiagen). SMARCAL1 was depleted using the MISSION esiRNA HUMAN SMARCAL1 (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... 6 million PBMCs were lysed using QIAzol Lysis Reagent (Qiagen). Samples were stored at -80°C until RNA extraction ...
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bioRxiv - Biochemistry 2023Quote: ... 6 mL of Ni2+-NTA slurry (Qiagen, Venlo, LI, Netherlands) were equilibrated in a gravity flow column with Wash Buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: ... UniSpike 6 (included in the QIAGEN miRCURY LNA RT Kit) to verify the efficacy of reverse transcription (RT ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was applied to a nickel-nitrilotriacetic acid agarose (Qiagen) column and washed with 20 mM imidazole for twice ...
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bioRxiv - Molecular Biology 2021Quote: ... Soluble fraction was incubated with Ni-nitrilotriacetic acid (NTA)-agarose (Qiagen) beads or glutathione-sepharose (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... but were purified using nickel-nitrilotriacetic acid resin (Qiagen, Germantown, MD). The final protein concentration was 15-260 μM (~2-10 mol ADP per mol kinesin) ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen) and the histidine tag was subsequently removed by thrombin digestion (10 U/mg of protein) ...
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bioRxiv - Neuroscience 2022Quote: Locked Nucleic Acid (LNA) oligonucleotides for C21orf2 were purchased from Qiagen. A complete list of the used ASOs can be found in extended data table 6 ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were purified using Ni-nitrilotriacetic acid (Ni-NTA; Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... which were purified by adsorption to nitrilotriacetic acid (NTA) agarose (QIAGEN) and elution with 25 mM NaH2PO4-150 mM NaCl-125 mM imidazole buffer (pH 8.0 ...
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bioRxiv - Genomics 2021Quote: ... These kits included QIAamp Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany), Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit (Norgen Biotek ...
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bioRxiv - Cell Biology 2020Quote: ... lysed and incubated with nickel-nitrilotriacetic acid beads (Qiagen, Hilden, Germany) as per the manufacturer instructions ...
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bioRxiv - Genomics 2020Quote: cfDNA was extracted using the QIAamp Circulating Nucleic Acid kit (Qiagen). Isolation of cfDNA was done from 1 mL of plasma ...
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bioRxiv - Microbiology 2023Quote: ... The eluted nucleic acid was purified using the DNeasy Kit (Qiagen) and analyzed with RT-PCR strong stop primers (SSS ...
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bioRxiv - Cancer Biology 2024Quote: ... and rEDA was purified using nickel-nitrilotriacetic acid-agarose resin (Qiagen).
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bioRxiv - Genomics 2024Quote: ... Nucleic acid isolation was performed using the QIAcube (Qiagen, Hilden, Germany) and the PAXgene blood miRNA kit according to the manufacturer’s protocol to extract gene-encoding mRNAs ...
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bioRxiv - Microbiology 2023Quote: ... The eluted nucleic acid was purified using the DNeasy Kit (Qiagen) and analyzed by qPCR with strong-stop primers (primers PR and PU5 ...