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Citations for Qiagen :
3001 - 3050 of 3857 citations for 6 Chloro 4 hydroxy 2 methyl 2H thieno 2 3 e 1 2 thiazine 3 carboxylic acid methyl ester 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: RNA was extracted from the supernatant of Vero E6 cells after 1 passage using the QIAamp Viral RNA Mini kit (Qiagen, https://www.qiagen.com/us/) without addition of carrier RNA ...
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bioRxiv - Genetics 2019Quote: ... DNA was isolated from 1 g of unexpanded leaves with CTAB method and purified with Genomic Tip 20/G (Qiagen K. K. Tokyo). The extracted DNA was sheared into 20 kb fragments using g-TUBE (Covaris ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then stained by Coomassie-blue or immunodetected as described before [26] using primary polyclonal antibodies directed against His6 epitope-tag (Penta His, Qiagen, dilution 1:1000), V5 epitope-tag (Bethyl Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... This was incubated in a 37 °C shaker for 1 hour before the addition of Proteinase K (10 µl, QIAGEN, >600 mAU / ml) and left at 55 °C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Lung homogenates were prepared by homogenizing 200 mg of tissue in a tube containing 1 mL DMEM and a steel bead with a TissueLyser LT (Qiagen, Germantown, MD, USA) for 30 s at 30 hertz ...
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bioRxiv - Neuroscience 2022Quote: PON2 level was quantified in the mouse whole brain homogenate in 100 mg per 1 ml of QIAzol® lysis reagent (Qiagen, Hilden, Germany). mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc. ...
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bioRxiv - Microbiology 2021Quote: ... approximately 1×106 Jurkat cells were washed twice in PBS and total DNA was extracted with the Qiamp® DNA Minikit (Qiagen, Manchester, UK). Quantitative PCR reactions were carried out as previously described using 0.3pmol each primer and 0.15 pmol of the probe in 25 μL volume containing 100-300ng total DNA using an ABI Prism® 7000 Sequence Detection System (SDS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 1 μg of total RNA was used for cDNA conversion using the RT2 first strand kit (Cat# 330404, Qiagen, Valencia, CA, USA). The prepared cDNA was diluted and used to determine the expression of genes of interest using specific primers ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 female and/or 1 male per litter) were homogenised and total RNA was extracted using RNeasy Mini Kits (liver; Qiagen, Chadstone, VIC, Australia) or Trizol reagent (muscle and adipose tissue ...
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bioRxiv - Genetics 2022Quote: Genomic DNA (gDNA) was extracted from 1-month-old Arabidopsis thaliana rosette leaves with the DNeasy plant mini kit (Qiagen, cat. no. 69104) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and 1:10 dilution of cDNA was used to quantify genes using SYBR Green I (QuantiFast® SYBR® Green PCR Kit, Qiagen, USA). ADP-Ribosylation Factor 1 (AB050957.1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time quantitative PCR was performed on 40 pg cDNA using specific primers (Supplementary Table 1) and miRCURY® LNA® miRNA SYBR® Green PCR (Qiagen). Values were normalized to miRNA quantity and expressed as relative expression to control WS using formulae 2−ΔCT.
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bioRxiv - Pathology 2024Quote: A sample of ca 1 mm3 excised from the middle section of left lateral liver lobe was cryo-homogenized using TissueLyser II (QIAGEN, Germantown, MD, USA), resuspended ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were washed once with 1×PBS after which DNA was isolated using the QIAamp DNA Mini Kit (Qiagen, Venlo, The Netherlands) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We verified the PCR products by 1% agarose electrophoresis with EtBr staining and purified using the QiaQuick PCR Purification Kit (Qiagen, Hilden, Germany, 28104) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from GFP-empty vector-transfected and GFP-Δ(1-240) JPh1-transfected C2C12 myoblasts using the RNeasy plus mini kit (Qiagen, Hilden, Germany), based on manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested (1×106) by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were weighted using an analytical scale and homogenized in 1 ml of deionized water using TissueRuptor II electric homogenizer by Qiagen (Germantown, MA, USA). RO5256390 was extracted using acetonitrile/water 50%/50% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... The DNA was separated on a 1% agarose gel and the appropriate band was excised and isolated using a gel extraction kit (Qiagen, Valencia, CA, USA). In-Fusion HD enzyme premix was added to the linearized PCR product and transformed into the Stellar™ Competent Cells (Takara ...
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bioRxiv - Microbiology 2019Quote: We extracted the DNA from mosquito E/F in the 1.5 ml collection tubes using the QIAamp DNA Micro Kit (QIAGEN, Germantown, Maryland) following a modified version of the manufacturer’s protocol as described elsewhere [38] ...
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bioRxiv - Biophysics 2021Quote: ... Supernatant was collected for 2.5 h binding with Ni-nitrilotriacetic acid resins (Qiagen) at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... His6-AHCY was isolated by Ni2+-nitrilotriacetic acid (Ni-NTA) affinity chromatography (Qiagen) using standard procedures and eluted with 50 mM Tris buffer pH 8.0 ...
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bioRxiv - Genomics 2019Quote: ... cfDNA was extracted from plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen) following the manufacturer’s protocol with minor modifications ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was mixed with nickel-nitrilotriacetic acid agarose beads (Qiagen, Hilden, Germany) and loaded on a column ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cfDNA was extracted from plasma using the QIAamp circulating nucleic acid kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The remaining lysates were incubated with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was mixed with nickel-nitrilotriacetic acid agarose beads (Qiagen, Hilden, Germany) and loaded on a column ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) and elution with imidazole ...
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bioRxiv - Genomics 2021Quote: ... Nucleic acids were extracted using a QIAGEN DNeasy (DNA) kit (Qiagen Hilden, Germany). Three de novo genome sequencing methods were performed on the liver fluke ...
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bioRxiv - Microbiology 2022Quote: Nucleic acids were extracted from samples using a DNeasy PowerSoil kit (Qiagen, Germany) and quantified using the Quant-IT PicoGreen assay kit (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... DNA from plasma was extracted with the QIAamp Circulating Nucleic Acid Kit (Qiagen).
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bioRxiv - Biochemistry 2019Quote: ... The protein was isolated by Ni2+-nitrilotriacetic acid (Ni-NTA) affinity chromatography (Qiagen). Proteins were eluted with 50 mM Tris buffer pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... Amino acid sequences were aligned using CLC Genomics Workbench 20.0.04 (QIAGEN, Aarhus, Denmark).
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bioRxiv - Genomics 2021Quote: Nucleic acids were extracted by homogenizing tissues using a TissueLyser II (Qiagen, 85300) followed by the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen ...
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Epigenetic alterations underlie airway macrophage differentiation and phenotype during lung fibrosisbioRxiv - Immunology 2020Quote: Nucleic acids were extracted from cells using the AllPrep Mini Kit (QIAGEN, Germany). DNA quality and quantity were assessed using Genomic DNA ScreenTape and TapeStation System (Agilent ...
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bioRxiv - Genomics 2020Quote: ... Chondrocytes were transfected in duplo with antisense locked nucleic acid (LNA) GapmeR (Qiagen) targeting P3H2-AS1 (TGAGCAACTAGGTGTA ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble recombinant protein was purified using theNickel-Nitrilotriacetic acid (Ni-NTA+; Qiagen) resin ...
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bioRxiv - Molecular Biology 2023Quote: Locked nucleic acid (LNA) GapmeR ASOs were custom-designed and purchased from Qiagen, USA ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial nucleic acid was extracted using the EZ1 DNA tissue kit (Qiagen, Germany) on EZ1 Advanced XL (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... all proteins were purified using Ni-NTA (nickel-nitrilotriacetic acid) resin from QIAGEN. After the incubation of Expi293 media supernatant with the Ni-NTA resin ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were first purified using nickel-nitrilotriacetic acid (NTA) agarose resin (Qiagen), and the His8-SUMO tag was then removed by TEV protease (10:1 w/w ...
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bioRxiv - Microbiology 2023Quote: ... viral nucleic acids were extracted using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH ...
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bioRxiv - Cancer Biology 2024Quote: Locked nucleic acid GapmeR antisense oligonucleotides (LNA ASOs) targeting LINC01432 (Qiagen, cat# 3653410) and CELF2 (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was incubated with Ni-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, Germany) on a rocker for 1 hour at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... These leftover materials were collected by LCM and placed into 350 μl of RLT lysis buffer (RNeasy, Qiagen, including 1%β-Mercaptoethanol (β-Me)) followed by RNA extraction and examination with the 2100 Bioanalyzer.
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bioRxiv - Plant Biology 2020Quote: ... Frozen cells were disrupted by bead beating at 30 h/z for 1 min before purifying total RNA using an RNeasy® plant mini kit (Qiagen™; ref. 74904) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Samples were subjected to dry pulverization before being homogenized in buffer (350 μL of Qiagen RLT buffer with 1% β-mercaptoethanol; Qiagen, Germantown, MD, USA). The homogenate was transferred to an RNase-free 1.5 mL centrifuge tube ...
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bioRxiv - Microbiology 2019Quote: DENV viral RNA extraction was performed from acute serum samples (Baseline, 1-10, and 15 dpi) using QIAamp Viral RNA mini kit (Qiagen Inc, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysate was cleared by centrifugation (35,000 x g, 1 h) and the supernatant was loaded onto a Ni-affinity chromatography column (Qiagen Ni-NTA Superflow, 20 mL) pre-equilibrated with Ni-NTA buffer ...
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bioRxiv - Physiology 2023Quote: RNA was extracted from MLEC and lung tissue by lysis in RLT buffer containing 1% β-mercaptoethanol for RNA isolation and subsequently purified using the RNeasy Mini Kit (Qiagen, 74106, Hilden, Germany) according to manufacturer’s instructions ...