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Citations for Qiagen :
2801 - 2850 of 3706 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 1 μl of diluted (0.08x) FastSelect rRNA HMR (Qiagen, Germany) and 1 μl of diluted (0.08x ...
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bioRxiv - Genomics 2020Quote: ... 1 μl of diluted (0.08x) FastSelect rRNA HMR (Qiagen, Germany) was added into 6 μl COVID-19 specimen RNA along with 3 μl NA denaturation buffer ...
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bioRxiv - Microbiology 2020Quote: ... 1 µg/ µl BSA and 10X HotStartTaq Master Mix (Qiagen). Cycling conditions included initial denaturation at 95°C for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... Tris 10 mM) supplemented with 1% Proteinase K (Qiagen #19133) and 1% RNAse A/T1 (ThermoFisher #EN0551 ...
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bioRxiv - Microbiology 2023Quote: ... with the oligonucleotides for THP-1 cells (obtained from Qiagen) and P ...
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bioRxiv - Biophysics 2024Quote: ... or subjected to immunoblotting with either Penta·His (1:1,000; QIAGEN) or Anti-c-Myc (1:5,000 ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was extracted from 1✕106 PBLs (Qiagen, 75144) and a reverse transcription reaction was performed to produce the DNA copy (Roche ...
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bioRxiv - Microbiology 2023Quote: ... et al.[1] was performed as described using HotStarTaq (Qiagen). A Poc 18S rRNA plasmid (MRA-180 ...
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bioRxiv - Cell Biology 2023Quote: ... TAZ or KIF3A from Qiagen (listed in Supplementary Table 1). After transfection ...
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bioRxiv - Genomics 2023Quote: ... 1 µl carrier RNA (1µg, from Qiagen, cat. no. 59824), 4 µl of Dr.GenTLE precipitation carrier (Takara ...
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bioRxiv - Genetics 2023Quote: ... and 1 uL of FastSelect rRNA-depletion enzyme (Qiagen #335377). To complete the fragmentation and deletion process ...
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bioRxiv - Microbiology 2024Quote: ... and HSV-1 was extracted using QIAamp DNA Kits (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and mouse anti-STREP II (QIAGEN, #34850; dilution 1:1000) antibodies were used as primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... leaf-disc punches were collected from plants and ground in 250 µL of 10 mM MgCl2 using the TissueLyser II (QIAGEN; 2 cycles of 30 seconds at 25 Hz) and 3-mm zirconium oxide beads (Glen Mills Inc.) ...
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bioRxiv - Neuroscience 2022Quote: ... Mice received either miRCURY LNA power inhibitor for mmu-miR-335-5p (Ant-335; sequence: 5’-CATTTTTCGTTATTGCTCTTG-3’; Qiagen, Cat No.: 339132; 0.1 nmol in 2 μL PBS), or a non-targeting scrambled control (Scr ...
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bioRxiv - Evolutionary Biology 2020Quote: All genomic DNA was extracted using a Bioline Isolate II Genomic DNA Extraction Kit (Cat#. BIO-52067) following the recommended protocol with an additional DNAse free RNaseA (100mg/ml) (Qiagen cat. #19101) treatment before column purification ...
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bioRxiv - Genetics 2020Quote: ... were washed with 5 ml PBS twice and the total RNA was isolated using a RNeasy Mini Kit (74104; Qiagen, Hilden, Germany). The solution was subsequently treated with DNase I (79254 ...
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bioRxiv - Microbiology 2021Quote: ... ethanol-stored flies were rehydrated in water and transferred to 1.5 ml well plates for homogenisation using a bead beater (Qiagen Tissue Lyzer II). Protein was digested using Proteinase K ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was further treated with 25 µL of proteinase K (20 mg/mL) (Qiagen Singapore Pte. Ltd, Cat. No. 19133), gently mixed by flicking of tube and incubated at 56 °C for 20 min ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged proteins were recovered from the clarified lysate using a drip column containing a 5.0 ml bed volume of Ni-NTA resin (Qiagen, Mississauga, ON, Canada). After extensive washing with buffer A ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from 3 ml of each raw milk sample using the PowerFood™ Microbial DNA Isolation Kit (MoBio Laboratories, Qiagen, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... the root and leaf dry matter were ground into powder by placing the samples into glass vials with three opposing blades and shaking at a frequency of 30 Hz for 10 minutes using a Qiagen TissueLyser II (Qiagen, ML, USA). Ground root and leaf percent N was determined by the Dumas method using the Elementar rapid N exceed analyzer (Elementar Americas Inc ...
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Cell-free DNA as a biomarker for prostate cancer: elevated concentration and decreased fragment sizebioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from 7 to 55 mL of plasma using the Qiagen QIAamp Circulating Nucleic Acid Kit (Qiagen, Redwood City, CA), and double eluted with 40 μL of Qiagen Elution Buffer ...
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bioRxiv - Microbiology 2022Quote: ... aeruginosa cells were lysed after 18 hours using 2.5mg/mL lysozyme and incubated at 37°C for 30 minutes and RNA was extracted using the RNeasy mini kit (QIAGEN, Germantown, MD). DNase treatment was done twice for better DNA removal using Qiagen RNase-free DNase during RNA extraction and using NEB DNase after RNA extraction ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was applied to a nickel-NTA affinity column containing a total of 4 ml bed volume of nickel-NTA superflow resin (Qiagen, Venlo, Netherlands). After washing with a washing buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: PBMCs were brought up to 15 mL volume in cold PBS and DNA was isolated with the DNeasy Blood & Tissue Kit (Qiagen catalog # 69504) according to the manufacturer’s protocol with 1 hour of incubation at 55°C for digestion ...
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bioRxiv - Bioengineering 2021Quote: Total RNA content of 3-4 biological replicates of control and samples treated with TNF-α at 40 ng/mL for 24 h were extracted using the RNeasy mini kit (QIAGEN, 74134) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... High-molecular-weight (HMW) genomic DNA from cell pellets of 1.6 ml overnight cultures was extracted using MagAttract® HMW DNA Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions and using a final elution volume of 100 μl ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was cleared by centrifugation at 18000 rpm at 4°C and the supernatant was loaded onto 5 mL Ni–NTA resin (Qiagen, Milan, Italy) equilibrated with binding buffer ...
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bioRxiv - Immunology 2020Quote: Dissected specific areas from each three MFPE sections were measured and introduced into RNase-free 0.5 ml Eppendorf tubes with buffer PKD from the miRNeasy FFPE Kit (Qiagen, Valencia, CA, USA). Direct microdissection of IHC stained MFPE sections were attempted but failed to provide RNA with enough quality and yields for further microfluidic quantitative PCR assays.
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bioRxiv - Immunology 2020Quote: Dissected specific areas from each three MFPE sections were measured and introduced into RNase-free 0.5 ml Eppendorf tubes with buffer PKD from the miRNeasy FFPE Kit (Qiagen, Valencia, CA, USA). Direct microdissection of IHC stained MFPE sections were attempted but failed to provide RNA with enough quality and yields for further microfluidic quantitative PCR assays.
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bioRxiv - Microbiology 2022Quote: ... 2 g of soil was immediately transferred into pre-weighed sterile tubes containing 3 ml of LifeGuard soil preservation solution (Qiagen, Toronto, CA) to stabilize the RNA ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from 3 mL overnight cultures of each strain (see Strains Cultivation) using the DNeasy PowerSoil Pro Kit from Qiagen (Hilden, Germany) per manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... IFAS and LifeGuard Soil Preservation were transferred into a 5 mL bead beating tube from DNeasy PowerWater kits for 5-minutes of vortexing (Qiagen, Hilden, Germany). Afterwards ...
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bioRxiv - Genetics 2023Quote: ... 1 mL glycerol stocks were made for each library and a 200 mL LB-AMP outgrowth was grown to a concentration of 3 OD600/mL followed by a Qiagen MAXI plasmid prep (Qiagen Cat#12963).
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bioRxiv - Systems Biology 2023Quote: ... for genomic DNA extraction using 1.5 mL of cell culture with a phenol chloroform extraction and phase lock tubes (Qiagen Sciences, Germantown, MD) to enhance phase separation ...
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bioRxiv - Microbiology 2023Quote: Cultured cells were washed once in 10 ml PBS before RNA extraction using the RNeasy Plus Micro kit (Qiagen, Cat. No. 74034). RNA was eluted in 30 µl RNase free H2O and stored at -80◦C until use.
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bioRxiv - Microbiology 2023Quote: ... Single colonies were picked and grown overnight in LB broth with 50 μg/mL Kanamycin before plasmids were isolated using a QIAprep Spin Mini Kit (Qiagen, Germantown, MD), confirmed by restriction enzyme digest ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was separately extracted from each 10 mL skinning sample using the DNeasy® mericon® Food Kit (Qiagen, Hilden, Germany). Each extraction was performed following the manufacturer’s instructions for standard large-scale extractions with minor modifications ...
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bioRxiv - Microbiology 2023Quote: ... each Sterivex filter membrane was sectioned in half (representing approximately 500 ml of sample) and placed in Qiagen DNeasy Powersoil DNA isolation kit bead-beating tubes (Qiagen; Hilden, Germany) for immediate DNA extraction and the other half for storage at -80 °C ...
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bioRxiv - Microbiology 2023Quote: ... a 1 mL aliquot was sterile filtered and kept to test conditioned media for IL-10 induction (as above)) and replaced with 5 mL of RNA-protect (Qiagen, Cat. # 1018390), mixed by pipetting ...
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bioRxiv - Biophysics 2024Quote: ... The clarified lysate was then applied to a 10 mL bed volume Nickel nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, cat. no. 30230) column ...
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bioRxiv - Microbiology 2020Quote: ... respectively34 (Table 1) were analyzed on the Rotor-GeneQ machine (Qiagen) using the following PCR conditions ...
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bioRxiv - Cell Biology 2022Quote: To synthesize cDNA from 1 μg RNA the Quantitect kit (Qiagen) was used according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: RNA (1 µg) was extracted with the RNeasy Kit (QIAGEN, 74106) and treated with DNase I following the manufacturer’s instructions (QIAGEN ...
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bioRxiv - Developmental Biology 2019Quote: ... collected with PBS 1% SDS and passed through Qiashredder columns (Qiagen) to disrupt nucleic acids ...
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bioRxiv - Biophysics 2019Quote: ... and probed with primary antibodies anti-5His monoclonal (1:1,000; QIAGEN), anti-Myc monoclonal (1:1,000 ...
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bioRxiv - Genomics 2019Quote: ... Each reaction contained 1× Rotor-Gene SYBR Green PCR Mastermix (Qiagen), 0.2 μM of primers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or Junctional Adhesion Molecule 1 (JAM1) using Polyfect (Qiagen, CA, USA) and selected in 1000 µg/ml G418 for stable transfectants ...
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bioRxiv - Microbiology 2020Quote: ... PCR#1 product was purified using Minielute PCR purification kit (Qiagen) and a second round of PCR amplification was performed with gene-specific primers R2 and forward F2 ...