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Citations for Qiagen :
2751 - 2800 of 3706 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Cells were selected in puromycin (1 μg/ml) the next day and after 4 days genomic DNA was extracted using DNeasy Blood and Tissue kit (Qiagen). Genomic DNA was sonicated to an average size of 500 bp using S2 Focused-ultrasonicator (Covaris ...
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bioRxiv - Immunology 2023Quote: ... A 0.3 ml portion of this homogenate was used to extract and purify RNA using the RNeasy Mini Kit (CAS: 74104, Qiagen) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Then samples and inputs were incubated for 4 hr at 37°C with 1.5 µl of 10 mg/ml RNase A (Qiagen, 1007885) and 15 µl 10% sodium dodecyl sulfate and 3.5 µl 20 mg/ml Proteinase K (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... The BmAgo3 immunoprecipitated beads were divided into two and incubated in buffer D with or without 200 μg/ml RNase A (Qiagen) at 30°C for 15 min ...
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bioRxiv - Genomics 2024Quote: DNA was extracted from approximately 3 ml of whole blood using the Gentra Puregene Blood Kits (#158467; Qiagen, Hilden, Germany), following the “Whole Blood” subsection in the manufacturer-provided handbook ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were decrosslinked and treated with proteinase K (0.1 mg/ml) overnight followed by genomic DNA extraction with QIAGEN Blood & Cell Culture DNA Maxi Kit (QIAGEN; 13362) and quantified by Qubit (Thermo-Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Parasite isolates were grown in Schneider culture medium until the end of the log phase at the Oswaldo Cruz Institute (Rio de Janeiro, Brazil. DNA was extracted from 107 – 108 parasites/ml using the QIAmp DNA Mini kit (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... to a density of 2 to 3 million cells /ml and then transfected with the MP-20 containing plasmid purified using a plasmid purification Giga kit (Qiagen). PEI MAX 40k (Polysciences ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was thoroughly aspirated and replaced with 20 µl of 1x PBS containing 5 mg/ml of RNase A (Qiagen) and 12 mM of purified H6-mNeonGreen-NLS protein as a loading control ...
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bioRxiv - Genomics 2021Quote: Amplicon libraries for viral genome sequencing were prepared using QIAseq FX DNA Library Kit and QIAseq SARS-CoV-2 Primer Panel (Qiagen, cat. no. 180475, cat. no. 333896) as instructed by the manufacturer’s manual ...
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bioRxiv - Developmental Biology 2020Quote: ... rhesus fibroblast cell line (n=1), pri-CTB (n=1, rh090419) and TSCs (n=3 rh121118, rh052318, cy091318) using a FlexiGene DNA Kit (Qiagen, cat no: 51206) and quantified using a Nanodrop™ One Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Colonies growing on the selective medium containing the antibiotic gentamycin (20 µg/ml) were checked by colony PCR and the plasmid DNA extracted using the QIAprep® Spin Miniprep kit (Qiagen). A restriction reaction was performed on the extracted plasmid DNA and the positive samples were sequenced ...
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bioRxiv - Plant Biology 2019Quote: ... the pellet was dissolved at 50 °C for 15 minutes in 19 ml of Buffer G2 (Qiagen, Germantown, MD, Cat #1014636).
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bioRxiv - Plant Biology 2019Quote: ... Bacteria were grown overnight at 37°C on LB plates with 50 μg/mL kanamycin and plasmids from randomly selected positive clones were purified using QIAprep Spin Miniprep kit (QIAGEN, Germany) and Sanger sequenced (Genoscreen ...
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bioRxiv - Molecular Biology 2019Quote: ... About 100 mg ground tissue sample was transferred into 1.5 ml tube for the extraction of total RNA using the Qiagen Plant RNeasy mini kit (Qiagen, Hilden, Germany). The RNA quality and quantity were evaluated by using Nanodrop (Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... We purified the digested vector from a 0.8% agarose gel (1.2 g agarose Fisher #21-255-00GM) in 150 mL 1x TAE (Fisher #FERB49)) using a QiaQuick Gel Extraction Kit (Qiagen #28706) and quantified using a Qubit dsDNA HS Assay Kit.
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bioRxiv - Molecular Biology 2021Quote: ... EGF (final concentration 20 ng/ml) was added for indicated times and RNA was extracted using an RNeasy plus kit (Qiagen, 74134) with DNase treatment according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: We extracted the DNA from mosquito E/F in the 1.5 ml collection tubes using the QIAamp DNA Micro Kit (QIAGEN, Germantown, Maryland) following a modified version of the manufacturer’s protocol as described elsewhere [38] ...
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bioRxiv - Cell Biology 2021Quote: Cells debris was pelleted by centrifugation at 23,000 x g for 45 minutes and the supernatant loaded onto a 10 ml Ni2+-NTA column (Qiagen, Hilden, Germany). The column was washed with 600 ml buffer A and protein batch eluted with 100 ml buffer B (buffer B = buffer A supplemented with 290 mM imidazole).
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bioRxiv - Cancer Biology 2022Quote: ... we cultured 250 ml Escherichia coli bacteria bearing transformed plasmids and isolated the plasmids using Qiagen Maxi-Prep Endotoxin-free kit (Qiagen; 12362) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The lysate was then clarified by centrifugation at 16,000 x g for 45 minutes and then applied to 3 mL Ni-NTA resin (Qiagen, Valencia, CA) equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2022Quote: RNA was extracted from liquid nitrogen-frozen cell pellets from 20 mL cultures grown to early stationary phase using the RNeasy Plant Mini Kit (Qiagen, Germany). Immediately after extraction ...
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bioRxiv - Microbiology 2022Quote: ... The solubilized membrane fraction was loaded on a column containing 0.5 mL Strep-tactin Superflow plus resin (50% suspension; Qiagen, Hilden, Germany) equilibrated with 4 mL of the Wash buffer ...
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bioRxiv - Genomics 2022Quote: ... The resulting pellet was gently resuspended in 20 ml of pre-warmed (37°C) G2 lysis buffer (Qiagen; Cat. no. 1014636), incubated with 50 μg/ml RNaseA (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... DNA extraction for sequencing was performed from 1.8 mL of overnight culture using the Qiagen DNAeasy UltraClean Microbial kit (Qiagen 12224-250). Extracted DNA was diluted to 10 ng/μL concentration (Qubit ...
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bioRxiv - Genomics 2022Quote: ... The swabs were placed in Red 1.5 mL RINO® screw-cap tubes (NextAdvance) pre-filled with RNase-free zirconium oxide beads and QIAzol Lysis Reagent (Qiagen) was added ...
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bioRxiv - Biochemistry 2019Quote: Total RNA extraction was performed on the cell pellet from 10 ml of algal culture using the RNeasy® Plant Mini Kit (QIAGEN). DNase treatment was carried out using TURBO DNA-free™ kit (Ambion) ...
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bioRxiv - Microbiology 2020Quote: ... were transferred into 0.2-mL PCR tubes and genome amplified using REPLI-g Advanced DNA Single Cell Kit (Qiagen Hilden, Germany). For the whole culture approach ...
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then incubated for ~ 10 min with 4 μg/ml of Penta•His biotin conjugate antibody (34440, Qiagen, UK) in reaction buffer [40 mM HEPES buffer (pH 7.3 ...
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bioRxiv - Genomics 2019Quote: ... Extraction using the CGP protocol (Moore et al., 2018) involved adding 30 µL of lysis buffer (1.25 µg/mL of Protease reagent (Qiagen; cat# 19155) in Tris HCl pH 8.0 ...
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bioRxiv - Genomics 2021Quote: ... was transferred using Pasteur blob to a clean Falcon tube containing 4.5 mL HEMAgene®•BUFFY COAT DNA stabilizing reagent (Oragene) and the DNA was extracted using QIAamp DNA Blood Mini Kit (Qiagen). The Coriell-NA12878 DNA sample was purchased from Coriell Cell Repositories ...
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bioRxiv - Immunology 2021Quote: ... Individuals were classified as Mtb-infected based on a positive QuantiFERON TB Gold In-tube assay (Qiagen; >0·35 IU/mL). All participants were healthy without signs or symptoms of active disease ...
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bioRxiv - Developmental Biology 2020Quote: ... the beads were transferred to a new 0.5ml tube and incubated in 100 μL ChIP elution buffer (10mM Tris-HCl pH8.0, 5mM EDTA, 300mM NaCl, 0.5% SDS) containing 5 µL proteinase K (Qiagen, 20mg/ml stock) at 55°C for 2 h and then at 65°C for 4 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA extractions were performed on cell pellets from 15 mL of these back-diluted cultures using QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from 50 mL wastewater samples within 24h of sampling using the DNeasy® PowerWater Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The samples were then transferred to a 15 ml tube and processed with a Qiagen Midi Prep Kit (Qiagen, Valencia, CA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: DNA was extracted from 200 ml of buffy coat using the Qiagen QIAamp DNA Blood Mini Kit (Qiagen, Cat No. 51106) according to the manufacturer’s instructions and including RNA digestion ...
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bioRxiv - Microbiology 2022Quote: ... The spooled DNA was washed with approximately 5 mL of 70% ethanol and dried before being suspended in 300µL of EB buffer (QIAGEN, Hilden, Germany). Purified DNA was quantified by Qubit (Thermofisher).
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bioRxiv - Evolutionary Biology 2023Quote: We extracted DNA by adding 3-5 mg of tail tissue to 150 µl of 10% ChelexR resin (Bio- Rad, Inc.) with 1.4 µl of Proteinase K (20 mg/ml, Qiagen, Chatsworth, CA), incubating at 55°C for 180 min ...
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bioRxiv - Cancer Biology 2023Quote: ... medium was removed and cells were lysed on the plate by addition of 3 ml of lysis reagent RLT Plus (Lot 169010027, Qiagen, Germany). 1 ml of lysate was transferred to a 2 ml Eppendorf tube ...
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bioRxiv - Synthetic Biology 2023Quote: ... The recombinant plasmid was then purified at a high concentration (200 μg/mL) using the plasmid preparation kit (Qiagen, Germantown, MD).
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bioRxiv - Systems Biology 2024Quote: ... The samples were then cooled for 5 minutes on ice and transferred to a pre-spun 15 mL MaXtract tube (Qiagen 129065) containing an equal volume of chloroform to the acid phenol used ...
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bioRxiv - Microbiology 2023Quote: ... Melted samples (different volumes from 300 to 1000 ml) were filtered on 0,22 μm pore size hydrophilic PVDF filters Durapore® and DNeasy Power Soil Pro® Kit (Qiagen) was applied for DNA extractions as is the most common and efficient method used in microbiome surveys51,67,68 ...
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bioRxiv - Systems Biology 2023Quote: ... The spheroblasted cells were collected in 50 mL Falcon tubes and spun for 5 min at 2,500 g and re-suspended in 7.5 mL of home-made buffer P1 (according to the manufacturers recipe, QIAGEN, Düren, Germany). 7.5 mL of home-made buffer P2 (according to the manufacturers recipe ...
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bioRxiv - Plant Biology 2024Quote: ... Colony PCR was performed with KOD One™ PCR Master Mix (Toyobo) to screen colonies before recovering plasmids with a 5 mL overnight culture and miniprep (QIAgen).
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bioRxiv - Physiology 2024Quote: ... 0.5% SDS and 20mg/ml of Proteinase K) and DNA purified using the DNeasy Blood and Tissue Kit (Qiagen, Venlo, Netherlands). PCR was performed with extracted DNA and primers for the recombined and non-recombined alleles as previously described [5] ...
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bioRxiv - Immunology 2024Quote: ... We collected 2.5 ml of CSF and whole blood for transcriptional profiling in blood PAX-gene® (Pre-AnalytiX, Qiagen, USA) tubes ...
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bioRxiv - Microbiology 2024Quote: ... using standards previously generated.65 We collected 2.5 ml of CSF and whole blood for transcriptional profiling in blood PAX-gene® (Pre-AnalytiX, Qiagen, USA) tubes ...
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bioRxiv - Cell Biology 2021Quote: ... Validated qPCR primers and primer assays (Qiagen, see Table 1) were used together with QuantiTect SYBR Green PCR kit (QIAGEN ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Penta-His-biotin conjugate 1:5000 (Qiagen) overnight ...