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Citations for Qiagen :
2601 - 2650 of 3706 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... 10 mM imidazole (final concentration) was added to the sample and the solution was bound to 0.5 ml NiNTA resin (Qiagen). The resin was washed with NTA buffer A1 (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... cells were first washed and pelleted five times with 15 ml of PBS then lysed with QIAzol reagent (Qiagen), RNA was extracted according to manufactures directions ...
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bioRxiv - Biochemistry 2021Quote: ... The ankyrin repeat domains were purified over a Ni-nitrilotriacetic acid column (2.5 ml column volume) according to the manufacturer’s instructions (QIAgen, Germany). Up to 200 mg of highly soluble ankyrin repeat domains were purified from one liter of E ...
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bioRxiv - Genetics 2021Quote: ... We extracted DNA from approximately 1-2 million cells of actively growing culture by first pelleting the cells and resuspending them in 1.0 mL Cell Lysis Solution (Qiagen). The samples were incubated with RNase A solution at 37°C for 40 min ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen pieces of tissue (~20 mg) were homogenized in 0.5 ml TRIzol in a TissueLyser II (Qiagen, Hilden, Germany) (2 × 2 min at 30 Hz ...
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bioRxiv - Immunology 2021Quote: ... or ectodomain buffer (20 mM Tris, pH 8.5, 500 mM NaCl) and applied to 10 ml Ni-NTA-Sepharose (Qiagen) columns ...
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bioRxiv - Microbiology 2021Quote: ... Samples were spun at 5000 x g for 10 minutes and supernatant was discarded before resuspension in 1.5 mL QIAzol reagent (Qiagen). Screw-cap tubes were prepared for each sample by adding ∼250 µL of sterile beads and the QIAzol suspension mixture was added and incubated for 5 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was removed to a fresh 50 mL conical and further incubated with 2mL of Talon Beads (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells suspended at 0.8 × 105 cells/ml were transfected with 50 nM siRNA using HiPerfect transfection reagent (Qiagen) according to the manufacturer’s protocol and analysed after 48 h ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from a 0.5 ml aliquot of the culture using the RNAeasy Protect Bacteria Mini Kit (74524, QIAGEN) with on-column DNAse digestion ...
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bioRxiv - Cancer Biology 2022Quote: ... The femurs were placed with knee-end down in a perforated 0.6 ml tube added 80 μl of RNAlater (Qiagen) and inserted in a 1.5 ml centrifuge tube ...
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bioRxiv - Immunology 2022Quote: His-tagged RBD of SARS-CoV S was diluted to a concentration of 0.8 μg/mL in Tris-buffered saline (TBS) and immobilized on NiNTA 96-well plates (Qiagen) for 2h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... cfDNA was extracted from 0.5 ml of plasma or serum using the QIAmp DNA Mini Blood kit (Qiagen, CA), according to the ‘‘Blood and body fluid protocol’’ and our own detailed protocol [37] ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble fraction was recovered by centrifugation at 40 000 × g in a JLA25.50 rotor and put through a gravity flow column with 5 ml of NiNTA Agarose (Qiagen). Bound fraction was washed in Buffer B (300 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... All samples were homogenized in PBS (1 mL for cecal content and 0.5 mL for fecal pellets and mLNs) for 2.5 min at 25 Hz in a TissueLyser (Qiagen, Germany). In the case of mLNs ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were filtered through a 0.22 µm syringe filter before application to 5 ml of nickel resin (Ni-NTA Superflow, QIAGEN) equilibrated in loading buffer (25 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer in a 50 mL falcon tube ...
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bioRxiv - Bioengineering 2024Quote: ... A subset of the samples (5 g) was preserved in 5 mL RNA preservation solution (RNAprotect, QIAGEN, Hilden, Germany) and stored at -80°C ...
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bioRxiv - Genomics 2024Quote: ... after the lysis step (step 4 in handbook protocol) and (ii) adding 700 mL of buffer RWT (Qiagen 1067933) instead of the provided RW1 (step 6 in handbook protocol).
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was filtered through a 0.45 µm syringe filter and applied to a 5 ml Ni-NTA agarose column (Qiagen) pre-equilibrated in Lysis-Wash buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... and genomic DNA was extracted from a 0.5 mL aliquot of whole blood with the DNeasy Blood and Tissue Kit (69506, Qiagen) according the protocal’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Plasmid transfections were performed in six-well tissue-culture treated dishes at 1.8 × 10^6 cells/ml using Effectene (301427; QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Both biopsies were homogenized in 350 mL of Buffer RLT and then extracted using the RNeasy Micro kit (Qiagen) with on-column DNase digestion ...
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bioRxiv - Neuroscience 2023Quote: ... The filtrated supernatant containing His-thioredoxin-ApoE was applied to a 5 ml Ni-NTA Superflow Cartridge (Qiagen, Germany) pre-equilibrated with TBS buffer A ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and its genomic DNA was amplified in the same 0.2-mL PCR tube using the REPLI-g single-cell WGA kit (QIAGEN) to obtain single-worm WGS data from LJ2050 ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was transformed into stbl3 competent E.Coli and DNA was harvested from 200 mL growth media using an endotoxin free plasmid purification kit (Qiagen).
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 5 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were moved on magnet and supernatant was transferred to clean 1.5 mL tubes for nucleic acid extraction using the MinElute PCR Purification Kit (Qiagen). Purified DNA was used for library preparation using the NEBNext Ultra Library prep Kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by three washes with 2X SSC at 37°C and treatment with RNaseA (200ng/ml; Qiagen) in 2X SSC at 37°C ...
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bioRxiv - Microbiology 2023Quote: The supernatant was collected and at room temperature was passed over a 2.0 mL bed volume of Ni-NTA Sepharose resin (Qiagen). The column was then washed three times with 10.0 mL of wash buffer (50.0 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was centrifuged at 15,000 x g for 35 mins and the soluble fraction incubated with 3 mL of Ni-NTA beads (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Deparaffinization with Xylene and RNA extraction was performed in sterile 1.5 ml microcentrifuge tubes with the RNeasy FFPE Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was 0.2 µM filtered and was incubated overnight at 4°C with 5 mL of Ni-NTA beads (Qiagen). XXX
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bioRxiv - Synthetic Biology 2022Quote: ... 0.1 mg/mL Lysostaphin (MilliporeSigma) was added in the pre-treatment step in combination with enzymatic lysis buffer (Qiagen). Bacterial strains are listed in Table S2.
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bioRxiv - Synthetic Biology 2022Quote: ... 0.1 mg/mL Lysostaphin (MilliporeSigma) was added in the pre-treatment step in combination with enzymatic lysis buffer (Qiagen). Purified DNA was stored at −20°C before further processing for NGS or cell-based detection.
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bioRxiv - Cancer Biology 2023Quote: DNA was extracted from fecal and cecal samples following 40 min of vortexing in 0.5 ml of Qiagen PM1 buffer (Qiagen) with 200 mg of 106/500 μm glass beads (Sigma ...
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bioRxiv - Immunology 2023Quote: ... The protein complexes were adjusted to 8.6–12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting total cell lysate was clarified by centrifugation (30,000 g for 25 min) and the supernatant fraction was applied to a 4.5 mL packed Ni-NTA resin (Qiagen) and gently rocked at 4 °C for 1 h in 50 mL conical tubes ...
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bioRxiv - Cell Biology 2023Quote: ... Ribosome pellets were resuspended in 0.7 ml Qiazol and mRNA was recovered using the miRNeasy RNA extraction kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... Transformants were grown to saturation in 5-8mL of LB media supplemented with ampicillin (100µg/mL) and miniprepped (Qiagen) for Sanger sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were clarified by centrifugation at 20,000 x g for 30 min and loaded on a 5 ml Ni-NTA Superflow column (QIAGEN) followed by extensive washing ...
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bioRxiv - Biochemistry 2023Quote: ... The filtrated supernatant containing His-tagged NanoLuc was applied to a 5-mL Ni-NTA Superflow Cartridge (Qiagen, Germany) pre-equilibrated with TBS buffer A ...
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bioRxiv - Bioengineering 2024Quote: Ten millilitres of culture were pelleted by centrifugation (5,000 × g for 3 min at 4°C) and resuspended in 5 mL of RNAlater (76106; Qiagen). Samples were stored at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... The culture was then combined with LB + 25 µg/mL chloramphenicol + 0.2% glucose (w/v) culture for overnight growth (37 °C) and then miniprepped (Qiagen).
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bioRxiv - Biochemistry 2024Quote: ... High titre baculovirus was produced by transfecting bacmid DNA into Sf21 cells at 0.5 × 106 cells/ ml cells using Superfect (Qiagen) in 24 deep-well blocks ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... beads were resuspended in 0.2 mL of 1xPBS supplemented with Proteinase K from the DNeasy Blood and Tissue Kit (QIAGEN) and incubated at 37 °C in a thermomixer for 4 h ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...