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Citations for Qiagen :
1751 - 1800 of 1802 citations for KGF 2 FGF 10 Human 171a.a since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Collected microvessels were pelleted at 20,000 g for 10 min and resuspended in 350 μl of RLT Plus Buffer from the RNeasy® Micro Kit (QIAGEN Inc.). Total RNA was isolated using the RNeasy® Micro Kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were weighed and homogenized in 1-10 µL/mg DMEM with a sterile glass bead at 30 Hz for 4 minutes using a TissueLyser (Qiagen, Germantown, MD) automated homogenizer ...
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bioRxiv - Microbiology 2023Quote: ... a 1 mL aliquot was sterile filtered and kept to test conditioned media for IL-10 induction (as above)) and replaced with 5 mL of RNA-protect (Qiagen, Cat. # 1018390), mixed by pipetting ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and RNA from patient tumor tissue biopsies and mouse tumors were extracted from 4 sections of 10 µm each from FFPE tumor blocks using the AllPrep® DNA/RNA FFPE kit (Qiagen #80234) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Hippocampal lysates were prepared by the addition of ice-cold PBS (200 µL PBS/10 mg hippocampal wet weight) and homogenized for 1 min in a TissueLyser II instrument (Qiagen, Hilden, Germany). One fourth of the obtained volume of each lysate was used for the analysis of total PLP concentrations as described below ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from 6 seedlings/sample of 10-days old Arabidopsis 24 h after the treatment with elicitors using the RNeasy Plant Mini Kit (QIAGEN, Hilden, Germany). Libraries were constructed using KAPA mRNA Capture Kit (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA removal was performed by applying 80 μl of DNase I working solution (10 μL DNase stock + 70 μL 1x RDD buffer; RNase-Free DNase Set, Qiagen, Hilden, Germany) to the column and incubated for 15 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The gene network analysis for the selected 10 marker genes was conducted using Ingenuity Pathway Analysis Software (Version 01-22-01; Ingenuity Systems, Qiagen, CA, USA). This analysis aligned the marker genes with established genetic pathways and networks ...
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bioRxiv - Developmental Biology 2024Quote: ... collected into 96-well plates (on ice) prior to the addition of 10 μL of RLT plus lysis buffer (Qiagen, Hilden, Germany). All instruments and surfaces were cleaned with 80% v/v ethanol ...
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bioRxiv - Genetics 2024Quote: ... First-strand cDNA synthesis was obtained from 10 ng of total RNA by using the miRCURY LNA RT Kit (Qiagen, Hilden, Germany) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in Propidium Iodide (PI) staining solution (1x PBS supplemented with 50 µg/mL PI, Invitrogen; 100 µg/mL RNase A, Qiagen; and 2 mM MgCl2) and incubated for 30 min at room temperature in the dark) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 ng of pFLAG expression vectors or empty plasmids and 50 ng psiCheck-2 plasmid were transfected using Effectene transfection reagent (Qiagen, Netherlands, Cat. No. 301425), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 20–30 mg of tissue was taken and placed into a 2 mL tube with 600 µL of RLT buffer with 1% β–mercaptoethanol and a 5 mm stainless steel bead (Qiagen, Hilden, Germany: #69989). Tissues were then dissociated using a Qiagen TissueLyser II (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... 80 µl of Viral Transport Media that had previously stored a nasopharyngeal swab from a patient infected with SARS-CoV-2 were used for RNA isolation using the QIAamp Viral RNA Mini spin kit (Qiagen, Cat No./ID: 52904) according to manufacturer specifications ...
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bioRxiv - Molecular Biology 2021Quote: ... Both the input and IP samples were incubated at 50°C for 2 hr and then cleaned up using the Qiaquick PCR Purification Kit (Qiagen, Hilden, Germany, Cat # 28104) and eluted in 35 μL of water.
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bioRxiv - Systems Biology 2019Quote: RNA was extracted from a total of 17 samples from Experiment 2 with the AllPrep PowerFecal DNA/RNA Kit (Qiagen USA, Cat. No. 80244). The 17 samples included 7 target samples taken during antibiotic treatment (4 untreated and 3 non-responder ...
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bioRxiv - Pathology 2021Quote: ... 20 – 30 mg of tissue were placed into a 2 ml tube with 600 µl of RLT buffer with 1% β-mercaptoethanol and a 5 mm stainless steel bead (Qiagen, Valencia, CA; #69989). Tissues were then dissociated using a Qiagen TissueLyser II (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the remaining 2 mL of the cell suspension using the RNeasy® Protect Bacteria Mini Kit (QIAGEN, Cat. No. 74524) following the manufacturer instructions.
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from bulk HSPC samples (∼2 x 106 cells per sample) using a QIAGEN Blood & Tissue DNA isolation kit (QIAGEN, Inc., Germantown, MD, USA) following the manufacturer’s protocol ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... placed into a 2 mL tube with 600 μL of RLT buffer with 1% β–mercaptoethanol and a 5 mm stainless steel bead (Qiagen, Hilden, Germany: #69989), then dissociated using a Qiagen TissueLyser II (Qiagen ...
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bioRxiv - Systems Biology 2020Quote: ... was prepared by centrifuging a cell culture at 5525xg for 2 minutes at 4°C then resuspending cells in 1mL of PBS-RNAprotect (333 μL RNAprotect Bacteria Reagent [76506, Qiagen, Hilden, Germany], 666 μL PBS). For immediate RNA stabilization by RNAprotect (Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... Approximately 30 mg of frozen tissue was placed into a 2 ml flat bottom centrifuge tube on dry ice with a 5 mm stainless steel bead (QIAGEN Germantown, MD, USA; Cat#69989) at the bottom ...
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bioRxiv - Microbiology 2019Quote: ... Reactions were run in a total volume of 10 μl having 5 μl Rotor-Gene SYBR® Green PCR Kit (Qiagen, NSW, Australia), 0.3 μl each of 10 μM forward and reverse primer and 2 μl of genomic DNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... total RNA and genomic DNA was extracted from flash frozen testes of a subsample of males 6 to 10 days following injection with the dsRNA using a Qiagen Allprep DNA/RNA Mini Kit (Qiagen, Venlo, The Netherlands). Expression levels for Dnmt1 was analyzed using qRT-PCR as described above.
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bioRxiv - Bioengineering 2021Quote: ... 7.5 μL of RNAse free H2O and 10 μL of 2X HotStarTaq™ Plus Master Mix (HotStarTaq™ Plus Master Mix Kit, Qiagen, USA). PCR conditions were an initial denaturation of 5 min at 95°C followed by 25 cycles of 30 s at 95°C ...
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bioRxiv - Cell Biology 2021Quote: ... This was incubated in a 37 °C shaker for 1 hour before the addition of Proteinase K (10 µl, QIAGEN, >600 mAU / ml) and left at 55 °C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... and from stool samples from 10 volunteers was extracted using QIAamp® PowerFecal® DNA Kit (QIAGEN, catalog no. 12830–50; Hilden, Germany). The genomic DNA was stored at m –20°C until PCR amplification and amplicon sequencing.
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bioRxiv - Microbiology 2021Quote: ... 1 μL of 20 mM of each primer (KAN-2 FP1 or KAN-2 RP1 complementary to the Tn5 sequence with the bubble primer 224) and 10 μL of the 10X Qiagen Multiplex PCR Master Mix Kit (Qiagen, Valencia, CA, USA) in a final volume of 100 μL ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA from 4-8 microdissected 10 μm FFPE sections were extracted using the QIAamp DNA FFPE Tissue kit (Qiagen, cat. no. 56404) according to manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: ... About 30-40 flowers were pooled together from 8-10 different plants for each biological repeat and RNA was isolated using RNAeasy plant mini kit (Qiagen, Germantown, MD, USA). RNA was converted to cDNA using PrimeScript RT Master Mix (Takara) ...
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bioRxiv - Biophysics 2022Quote: ... 100 µl of the protein extract were then supplemented with 400 µl of homogenization buffer containing 10 mM imidazole and added to 50 µl Ni2+-NTA agarose beads (Qiagen GmbH, Hilden, Germany) preconditioned with washing buffer (0.1 M sodium phosphate buffer (pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from between < 10 and 300 mg dry weight of gut content using the MoBio Powersoil HTP 96 Soil DNA Isolation Kit (Qiagen, Valencia, CA, USA) according to the DNA extraction protocol (v ...
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bioRxiv - Cancer Biology 2019Quote: FFPE samples were sliced into 10-μm sections and gDNA was extracted using the QIAamp DNA FFPE Tissue Kit (Qiagen, Sussex, United Kingdom). DNA concentration was determined using the Quant-iT™ PicoGreenR dsDNA Assay Kit (Thermo Fisher Scientific UK Ltd. ...
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Macrobdella decora: Old World Leech Gut Microbial Community Structure Conserved in a New World LeechbioRxiv - Microbiology 2019Quote: ... The supernatant was transferred to clean 1.5mL MCF tube and to which 350 μL ethanol at 4’C was added before vortexing for 10 sec and transferring the supernatant to a QIAamp Mini spin column (Qiagen Germantown, MD U.S.A.). The sample was process as recommended in the QIAamp DNA Mini Handbook and eluted with 10 mM Tris-Cl ...
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bioRxiv - Physiology 2020Quote: Total RNA was extracted from the gill samples collected from both Lake Winnipeg walleye (n = 10) and Delta Marsh walleye (n = 21) using the RNeasy Plus Mini Kit (Qiagen, Toronto, ON, CA) following manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: RNA was extracted from mouse islets (∼300 islets per sample, pooled from ∼10 mice of the same genotype) using RNAeasy Micro Kit (Qiagen, Valencia CA, USA) as per manufactures instructions ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from between < 10 and 300 mg dry weight of gut content using the MoBio Powersoil HTP 96 Soil DNA Isolation Kit (Qiagen, Valencia, CA, USA) according to the DNA extraction protocol (v ...
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bioRxiv - Microbiology 2022Quote: ... exposed to 0.4 nM and 4 nM of β-estradiol for 10 days) Vagina Chips using QIAzol lysis reagent (Qiagen, cat. no. 79306). Complimentary DNA was synthesized using a SuperScript VILO MasterMix (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... and 1:10 dilution of cDNA was used to quantify genes using SYBR Green I (QuantiFast® SYBR® Green PCR Kit, Qiagen, USA). ADP-Ribosylation Factor 1 (AB050957.1 ...
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bioRxiv - Neuroscience 2023Quote: ... A sample of the resulting homogenate (10-20 mg) was used to assess total cellular RNA using the RNeasy® Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was extracted from 5 to 10 million cells or 15 to 25 mg of tissue using the MagAttract HMW DNA kit (Qiagen N.V., Venlo, Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was extracted in a pooled reaction (up to 10 wasps from one brood per reaction) using the Qiagen DNAeasy blood & tissue kit (QIAGEN Inc, Hilden, Germany). After extraction DNA was amplified in a multiplex PCR reaction using fluorescently labelled Lysi07 primers ...
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bioRxiv - Genomics 2021Quote: Amplicon libraries for viral genome sequencing were prepared using QIAseq FX DNA Library Kit and QIAseq SARS-CoV-2 Primer Panel (Qiagen, cat. no. 180475, cat. no. 333896) as instructed by the manufacturer’s manual ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Lysates were incubated for 1 hr at 37°C after adding 10 µl of RNAse A (20 mg/ml) and proteins were precipitated with 200 µl of Protein Precipitation Solution (Qiagen Gentra Puregen Cell kit). After centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... Enzyme was inactivated at 95 ° C for 10 min and 700 μl Trizol was added followed immediately by RNA extraction using the miRNeasy kit (Qiagen Inc., Valencia, CA, USA).
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bioRxiv - Microbiology 2019Quote: DENV viral RNA extraction was performed from acute serum samples (Baseline, 1-10, and 15 dpi) using QIAamp Viral RNA mini kit (Qiagen Inc, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from a 10 mL culture using the QIAGEN® Genomic DNA Buffer Set and Genomic-tips™ 100/G set (midi-prep) (QIAGEN, Hilden, Germany). This procedure adhered to the Sample Preparation and Lysis Protocol for Bacteria as outlined in the QIAGEN® Genomic DNA Handbook ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 µl buffer RLT plus with 2-Mercaptoethanol (βME) at 10 µl βME/ml of RLT+ was added before placing the tube into a TissueLyser LT (QIAGEN Germantown, MD, USA; Cat#69980) set at 50 oscillations/second for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... and aged (n=10) RVLM-containing tissue punches was reverse transcribed to cDNA using an RT2 preamp cDNA synthesis kit (Qiagen, Inc., First strand cDNA synthesis) following manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... RNA and protein were digested in 40 mM Tris-HCl pH 6 and 10 mM EDTA by adding first RNAse A (Qiagen; #19101, 1.5 hours, 37°C), followed by Proteinase K (Thermo Fisher Scientific ...