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Citations for Qiagen :
1701 - 1750 of 1802 citations for KGF 2 FGF 10 Human 171a.a since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... cfDNA was extracted from 2 mL of plasma at baseline and from 3 mL of plasma post-treatment using QIAamp DNA purification kit (Qiagen, Venlo, the Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... followed by lysis in a 2 mL sure-lock tube containing 5 mm stainless steel homogenization beads using the TissueLyser LT (Qiagen, Germantown, MD, USA) for 30 seconds at 30 hz followed by 1 min of 30 hz while keeping the sample cold ...
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bioRxiv - Microbiology 2021Quote: ... transferred to the laboratory (within 2 hours) on ice and immediately processed for DNA extraction using the DNeasy PowerSoil kit (Qiagen, Valencia, CA, USA) according to manufacturer protocols ...
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bioRxiv - Cancer Biology 2019Quote: ... First-strand cDNA was prepared from 2 µg of cellular RNA in a total reaction volume of 20 µL using the reverse transcriptase Omniscript (QIAGEN, Mississauga, ON, Canada). After reverse transcription ...
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bioRxiv - Microbiology 2021Quote: Cellular total RNA was prepared from 2-4×106 cells for each sample using the RNeasy Plus Micro kit (Qiagen, Hilden Germany, #74034). For qRT-pCR ...
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bioRxiv - Microbiology 2021Quote: ... were selected with a P<0.05 and 2-fold change in gene expression and used for network/pathway analysis using Ingenuity Pathway Analysis (IPA; Qiagen, Valencia, CA, USA) as described previously (5).
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bioRxiv - Genetics 2020Quote: ... approximately 30 seeds per genotype were placed in a 2 mL Eppendorf Safe Lock tube along with one stainless steel bead (Qiagen Cat. No. 69989), frozen in liquid nitrogen ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Microsatellites were co-amplified in multiplex PCR (Table 2) with a Thermocycler T Gradient machine (Biometra, Goettingen, Germany) using the Qiagen® Multiplex PCR Kit (Qiagen, Hilden, Germany). Forward primers were 5’-labeled with fluorescent dyes HEX ...
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bioRxiv - Systems Biology 2019Quote: ... Approximately ∼50 mg frozen tissue was pulverised in methanol-chloroform (300 µl, 2:1 v/v) using a TissueLyser (Qiagen, West Sussex, UK). Then the mixture was sonicated for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA from untreated or RSL3-treated (with or without 2 hr pre-treatment with 2 µM Ferrostatin-1) MM1S and MM1R cells was extracted using the RNeasy Mini Kit (Qiagen, Venlo, the Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from peripheral blood leukocyte samples from visit 2 or 3 using the Gentra Puregene Blood Kit (Qiagen; Valencia, CA, USA) according to the manufacturer’s instructions (www.qiagen.com ...
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bioRxiv - Genomics 2021Quote: ... Quantification of ACE2 transcript levels was performed by preparing total RNA from 2-4×106 cells for each sample using the RNeasy Plus Micro kit (Qiagen, Hilden Germany, #74034). For qRT-pCR ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA was extracted from serially diluted and the 4 different media SARS-CoV-2 samples using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH, Hilden, Germany). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA extraction was performed from 25 mg (±2 mg) of arthropod powder with Qiagen Dneasy® Blood & Tissue extraction kit (Qiagen, Hilden – Germany) following the manufacturer’s protocol (see Sire et al ...
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bioRxiv - Cancer Biology 2021Quote: ... His-6X-tagged proteins (30 μg/10 μl beads) in 400 μl of PBS were incubated with Ni-NTA Magnetic Agarose Beads (QIAGEN, Hilden, Germany) at room temperature (RT ...
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bioRxiv - Genomics 2019Quote: DNA extractions were performed using ∼10 g of sediment with the PowerMax Soil DNA Isolation Kit (MO BIO Laboratories, Inc.; Qiagen, Hilden, Germany). To increase the DNA yield ...
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bioRxiv - Genomics 2019Quote: ... 10 μL of each PCR product were pooled and purified using three columns of a MinElute PCR Purification Kit (Qiagen cat # 28004) for a total elution volume of 30 μL ...
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bioRxiv - Plant Biology 2021Quote: ... Lyophilized material was pulverized in a 2ml screw top microcentrifuge tube with a glass bead for 10 minutes at a frequency of 20/s in a TissueLyser (Retsch ballmill, Qiagen, Venlo, Netherlands). The material was AIR prepped and destarched using protocols from Barnes and Anderson (Barnes and Anderson ...
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bioRxiv - Bioengineering 2020Quote: ... The RBD wildtype and mutant proteins of SARS-CoV-2 spike with 10×his tag at N terminal were expressed in HEK 293 and purified with Ni-NTA affinity columns (Qiagen, Hilden, Germany).
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bioRxiv - Neuroscience 2020Quote: ... along with the human NaV β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.X:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 g x 10 min) and from infected cells using QIAamp Viral RNA Mini Kit and RNeasy Plus mini kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Plant Biology 2020Quote: ... the root and leaf dry matter were ground into powder by placing the samples into glass vials with three opposing blades and shaking at a frequency of 30 Hz for 10 minutes using a Qiagen TissueLyser II (Qiagen, ML, USA). Ground root and leaf percent N was determined by the Dumas method using the Elementar rapid N exceed analyzer (Elementar Americas Inc ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA from cancer cells (cultured 48 hours in RPMI supplemented with 10% FBS) or primary tumors was extracted using an RNAeasy kit (Qiagen, Germantown, MD), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was resuspended in 30 μL of mQ water and incubated with 1 μL of RNase (10 mg/mL prepared from a stock of 100 mg/mL of RNase A from Qiagen, Darmstadt, Germany) at 37 °C for 1 h ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis of 100 ng total RNA was performed with miRCURY LNA RT Kit (10 µl volume reaction) which adds a 5’ universal tag of a poly(A) tail to mature miRNA templates (QIAGEN, Germantown, MD). cDNA template was diluted 1:10 ...
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bioRxiv - Genetics 2022Quote: ... The PCR amplification was carried out in a 10-μl reaction volume containing 8 μl of Taq PCR Master Mix (Qiagen, Hilden, Germany), 10 μM of each primer (1 μl) ...
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bioRxiv - Microbiology 2020Quote: ... 100 μL 0.1mm glass beads were added into the filter casing and put through two 10-minutes cycles of bead beating in a Tissuelyser (Qiagen, Hilden, Germany). The flow-through ...
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bioRxiv - Neuroscience 2021Quote: ... spun down (400 g × 10’ at 4 °C) and the pellet was resuspended in 600μ of Qiazol Lysis Reagent (Qiagen, Cat.No. 79306) and kept at −80 °C before sequencing.
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bioRxiv - Microbiology 2020Quote: ... 120 mg l-1 sodium pyruvate and 10% FCS (supplemented with 10% Fetal Calf Serum and 1% penicillin–streptomycin) at 300 Hz for two minutes using the Tissuelyser II (Qiagen, Hilden, Germany) and centrifuged to clarify the supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... and homogenized using a syringe equipped with a 26G needle (10 brains per sample) using the RNA XS Plus kit (Qiagen, Hilden, Germany). cDNAs were synthesized using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: Snap-frozen iBAT samples were homogenized in deionized water (200 µl per 10 mg tissue) using a TissueLyser bead mill (85210, Qiagen, Hilden, Germany). The glycogen content of the homogenates was measured using a Glycogen Assay Kit II (ab169558 ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were homogenized using a tissue homogenizer (Brinkmann Instruments, Model PT 10/35, 110 Volts, 6 Amps, 60 Hz) for spleen and thymus while RLT (Qiagen, Hilden, Germany) and hand homogenization was used to isolate BM ...
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bioRxiv - Molecular Biology 2019Quote: DNA was purified from the eluate and from 1% and 10% input chromatin using QIA-Quick DNA purification kit (Qiagen, Hilden, Germany) and eluted within 50 μl of ddH2O ...
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bioRxiv - Genetics 2019Quote: ... DNA was extracted from leaf tissue collected from 10 to 15 plants per line using Qiagen Plant DNeasy kit (Qiagen, Germantown, MD). DNA quantification was performed with Quant-iT Picogreen dsDNA assay kit (Thermo Fisher Scientific ...
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bioRxiv - Epidemiology 2019Quote: The PCR amplification mixtures used for RD9 deletions were as follows: reactions were performed in a total volume of 20 μl consisting of 10 μl HotStarTaq Master Mix (Qiagen, United Kingdom), 7.1 μl distilled H2O ...
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bioRxiv - Evolutionary Biology 2019Quote: Total DNA was extracted from tadpole tails by overnight digestion in 10% proteinase K solution at 56 °C and extracted using the Qiagen Biosprint 96 DNA blood kit (Qiagen, CA, USA). DNA was eluted in 100 - 200 μL buffer AE (QIAgen).
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was isolated from soleus muscle (10 µg) and apex of ventricle (20 µg) using RNeasy fibrous tissue mini kit (QIAGEN, Hilden, Germany) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: DNA was isolated from 10-20 8-μm frozen tissue sections of biopsies from humanized mice using the PureGene DNA isolation kit (Qiagen, Hilden, Germany). PCR was performed with six framework region 1 subgroup specific primers and a JH primer mix (3’ JH mix ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transiently co-transfected with NaV1.2 and the accessory β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.2:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...
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bioRxiv - Systems Biology 2021Quote: ... The liquid was aliquoted into a microcentrifuge tube with 590 µl nuclease-free water and 10 µl Proteinase K (QIAGEN, Hilden, Germany). For RNA extraction from swabs ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from cultures of LSUCC0096 that had reached max cell density (~106 cells mL−1) growing in JW1 medium (10) at room temperature using a MoBio PowerWater DNA Isolation kit (QIAGEN, Massachusetts, USA) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... of a 6-well plate in 200 μL ice-cold PBS + 10% Proteinase K and processed through the DNeasy Blood and Tissue Kit following manufacture protocols (QIAGEN, Hilden, Germany). gDNA was stored at −20° C for short-term storage or −80° C for long-term storage.
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bioRxiv - Microbiology 2020Quote: Total RNA extraction was performed using a squash buffer (10 mM Tris base, 1 mM EDTA, 50 mM NaCl) supplemented with 1:8 part Proteinase K (Qiagen, 15mg/ml). Mosquito abdomens with the midguts and heads with thoraces were individually collected in 50 μl of squash buffer at 7 and 14 days ...
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bioRxiv - Immunology 2021Quote: ... Samples were centrifuged at 14,000 g at 4°C for 10 mins and the supernatant collected for processing using a RNeasy mini-isolation kit (Qiagen Ltd, UK) according to the manufacturers’ protocol ...
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bioRxiv - Plant Biology 2022Quote: Total root RNA was extracted from plantlets grown in vitro at 22 °C and 10 °C using the RNeasy®Plant Mini Kit (QIAGEN, Germany). One microgram of total RNA was reverse transcribed using an oligo(dT)20 primer and the Super Script™ IV RT (lnvitrogen,USA ...
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bioRxiv - Microbiology 2022Quote: ... of a 6-well plate in 200 μL ice-cold PBS + 10% Proteinase K and processed through the DNeasy Blood and Tissue Kit following manufacture protocols (QIAGEN, Hilden, Germany). gDNA was stored at -20° C for short-term storage or -80° C for long-term storage.
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bioRxiv - Immunology 2022Quote: ... and cultured in the presence or the absence of the AHR antagonist CH223191 (10 µM) for 18h.Total RNA was extracted using RNeasy Plus mini kit (Qiagen, Germantown, Maryland, USA). Genome-wide transcriptional profiling was performed by Genome Québec (Montreal ...
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bioRxiv - Microbiology 2023Quote: Cultured cells were washed once in 10 ml PBS before RNA extraction using the RNeasy Plus Micro kit (Qiagen, Cat. No. 74034). RNA was eluted in 30 µl RNase free H2O and stored at -80◦C until use.
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bioRxiv - Molecular Biology 2023Quote: Total DNA was separately extracted from each 10 mL skinning sample using the DNeasy® mericon® Food Kit (Qiagen, Hilden, Germany). Each extraction was performed following the manufacturer’s instructions for standard large-scale extractions with minor modifications ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated following the Powersoil DNeasy kit protocol with the addition of a 10 min bead beating step at 50 s-1 (Qiagen, Hilden, Germany). RNA was isolated following the Ribopure Bacteria kit protocol (Thermo Fisher Scientific ...