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Citations for Qiagen :
1501 - 1550 of 1802 citations for KGF 2 FGF 10 Human 171a.a since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: RNA was extracted from appropriately staged embryos or whole larvae (n=10 /sample) using the RNeasy Plus Universal mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and 10 mM imidazole was added to the supernatant then loaded to a gravity column packed with Ni-NTA resins (Qiagen) pre- equilibrated with 20 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... or 1 μl 1:10 diluted oligonucleotides (for RNA from immunopurified ribosomes) from the QIAseq FastSelect –rRNA Yeast Kit (Qiagen) in 10.5 μl total at 75°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted from homogenized whole bodies with a Qiagen DNeasy kit after overnight treatment with 10% proteinase K (Qiagen) at 56 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The tissue was ground to a fine powder by adding 10-15 zirconia beads (1 mm diameter) and using a TissueLyser (Qiagen) for sunflower ligules ...
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bioRxiv - Microbiology 2019Quote: DNA for 16S rRNA gene sequencing was extracted from 10-50 mg fecal pellet from each mouse using the DNeasy Powersoil HTP 96 Kit (Qiagen) and an EpMotion 5075 automated pipetting system (Eppendorf) ...
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bioRxiv - Molecular Biology 2020Quote: ... after adding 1.0 x 10^8 copies/μL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Purified DNA was eluted in either 45 µl of EB buffer (10 mM tris-hydrochloride (pH 8.0) (Qiagen, The Netherlands) or 45 µl of TE buffer (10 mM tris-hydrochloride (pH 8.0) ...
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bioRxiv - Cancer Biology 2020Quote: DNA and RNA were extracted from the cervical cancer tissues (5-10 mg) using the AllPrep DNA/RNA Micro Kit (QIAGEN) as described by the manufacturer ...
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bioRxiv - Neuroscience 2019Quote: RNA extraction from 1-10 x104 FACS-sorted microglia or iPS-derived microglia-like cells was performed on the QIAcube (Qiagen) using the RNeasy Micro Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... Each reaction mix comprised 10 μL of 2x Rotor-Gene Multiplex PCR Master Mix (Rotor-Gene Multiplex PCR Kit, catalogue no. 204774; Qiagen), 1 μL of a mix of primers and probes (to give final concentrations of 0.5 mM each primer and 0.2 mM each probe) ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA was extracted from 10 ul of the purified virus using the MinElute Virus Spin Kit (Qiagen Cat#57704), and the viral genome titer was determined by qPCR using an AAV2 rep gene specific primer probe set (repF ...
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bioRxiv - Microbiology 2019Quote: ... All sequences were analysed using the BLAST program [27] and the variable regions were assembled using the CLC Genomics Workbench 10 (QIAGEN). The complete sequence of the integrons were deposited in the GenBank database ...
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bioRxiv - Immunology 2020Quote: ... and DNA from 7-10 clones from before and after FLPo-mediated recombination was prepared by miniprep (Qiagen, cat. 27106) and sequenced by Sanger sequencing with the primer GFP int F Age 66 ...
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bioRxiv - Immunology 2019Quote: Total RNA samples were diluted to a concentration of 10 ng/μl prior to gene expression studies by qRT-PCR using QuantiFast SYBR green RT-PCR kit (Qiagen) in 7900HT Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Physiology 2019Quote: ... 72 °C - 10 min) and amplicons separated using a Qiaxcel Advanced Separation System using 15 - 3000 bp markers (Qiagen, UK).
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bioRxiv - Immunology 2019Quote: ... each fluorescent cell was pipetted up and down the micropipette three times to remove unwanted contamination before being ejected into 10 μl of cold RLT (Qiagen) supplemented with 1% β-mercaptoethanol (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Nuclei pellets were centrifuged for 10 min at 1500g and processed immediately for gDNA extraction using a QIAamp DNA micro kit (Qiagen) according to manufacturer instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Cells attached to the collagen matrix were fixed with 4% PFA for 10 minutes at room temperature for IF analysis or lysed with RLT buffer (Qiagen) for RNA isolation and qRT-PCR analysis as described above.
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bioRxiv - Biophysics 2021Quote: ... PNGase F and the cleaved 10 × His tag were removed by passing the sample through Ni-NTA superflow resin (QIAGEN). The receptor was concentrated to 20–30 mg/ml with a 100 kDa cut-off concentrator (Millipore) ...
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence positive cells were collected in a round-bottom polystylene tube that contains 150 - 200 μl RLT buffer with beta-mercaptoethanol (10 μl / 1 ml RLT buffer, RNeasy micro kit protocol, Qiagen). Cell sorting was performed for about 15 min and collected cells were immediately frozen on dry ice and kept in −80 °C for up to one week ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from cells collected from 10 mL late-exponential phase culture in Todd-Hewitt broth with the DNeasy Blood & Tissue kit (Qiagen) using a modified protocol with an additional lysis step as described previously 61 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were seeded at 50% confluency in a 10 cm dish and transfected with 200 pmol siRNA (Qiagen, Germantown, MD) using RNAiMax Lipofectamine (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Two days after infection the mice were sacrificed and the distal 10 cm of the small intestine was dissected and shortly incubated in RNA later (Qiagen). Then the tissue was flushed with ice-cold DPBS to remove the fecal content and opened longitudinally on ice using scissors ...
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bioRxiv - Genomics 2021Quote: ... a 1.0 μL aliquot of each sample was amplified in 10 μL reactions incubated at 30°C for 16 h as described in the REPLI-g Mini Kit (QIAGEN).
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from vpr+ and vpr- cells 10 days post-infection using Qiagen DNeasy Blood & Tissue kit (Qiagen) and 200 ng of DNA was sheared to 1 kb fragments using Covaris M220 and micro- TUBE according to the manufacturer’s recommended settings (Covaris ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from cells (6-well dishes) or liver pieces (∼10-15mg) using the RNeasy Plus Mini kit (Qiagen). For livers ...
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bioRxiv - Cancer Biology 2021Quote: RNA from infected cell lysates was harvested by incubating infected Vero E6 cells for 10 minutes with RLT buffer (Qiagen) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 % (v/v) glycerol) for 30 s at a frequency of 15 s-1 using a TissueLyser II (QIAGEN). As the tissue homogenates were to be used for both native and SDS PAGE ...
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bioRxiv - Cancer Biology 2022Quote: DNA was extracted from 10 micro-meter slices using the Qiagen GeneRead DNA FFPE extraction kit (Qiagen, Catalogue no. 180134) which includes a Uracil N Glycosylase enzyme treatment to reduce FFPE artefacts ...
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bioRxiv - Biochemistry 2022Quote: ... were purified with 25 μL of Mag-bind Total Pure NGS beads and normalised in the same step by taking 10 μL of each reaction after resuspension (Qiagen Elution buffer ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed in TE buffer (pH=8) supplemented with 1 mg/ml of lysozyme and 10 µl of proteinase K (Qiagen) for 10 minutes at room temperature with a shaking of 500 rpm ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from approximately 10 g of sediments for each depth using PowerMax Soil DNA Isolation Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... We extracted total RNA from 10 leaves that were shorter than 500 µm and from 4–6 mature leaves using the RNeasy Micro Kit (Qiagen) and the RNeasy Plant Mini Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was extracted from pooled (n=10) embryonic zebrafish and pooled (n=3) adult (> 1 year) heart samples by QIAGEN RNEasy Lipid Tissue Extraction kit according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... the manufacturer’s directions were followed with the exception of adding 500ul of 10% SDS (w/w) to the homogenate at 60°C for 20 minutes before the addition of P2 buffer (Qiagen). This allowed the brain tissue to better solubilize with the Qiagen buffers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 x g for 10 min) and infected cells using a QIAamp Viral RNA MiniKit and RNeasy Plus mini kit (Qiagen), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... 10∼12 ml cell culture medium was subjected to extracellular RNA isolation using the exoRNeasy Maxi kit (Qiagen, Cat# 77164) with minor modifications ...
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bioRxiv - Microbiology 2022Quote: ... with heat inactivation at 75°C for 10 minutes and DNA was further removed using a RNeasy Mini kit (Qiagen). RNA was harvested from three independent cultures as biological replicates per experiment ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from cryopreserved PBMCs at 7 different time-points (10, 33, 56, 94, 120, 185, and 198 wpi) using the AllPrep DNA/RNA mini kit (Qiagen) as per the manufacturer’s specifications ...
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bioRxiv - Microbiology 2022Quote: For RNAseq the pellets were resuspended in 150 µL 10 mM Tris-HCl pH 8 and mixed with 700 µL of ice cold RLT+BME (RLT buffer (Qiagen) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2022Quote: ... Red blood cells were eliminated by incubation for 10 min on ice with 5 mL of erythrocyte lysis buffer (Qiagen). Human cells were enriched using the Mouse Cell Depletion Kit and the QuadroMACS magnetic cell separator (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: ... as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000, Qiagen) for one hour ...
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bioRxiv - Molecular Biology 2019Quote: Individual single Giardia cysts or pools of 10 cysts isolated by FACS were subjected to MDA with the REPLI-g single cell kit (Qiagen). MDA was performed according to the manual ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 1×10^7 PBMCs using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Hilden, Germany). Following nucleic acid isolation ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen right hemibrain was crunched in liquid nitrogen and 10-20mg of pulverized mouse brain was used for RNA extraction with RNeasy Plus Mini Kit (Qiagen). RNA concentration and quality were determined using Agilent RNA 6000 Nano Kit according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Glass beads (3mm) and 1 mL of sterile water were added for homogenization at 30Hz for 10 minutes (TissueLyser II, Qiagen). Homogenates were diluted with PBS and inoculated onto SAB plates and incubated for 3 days at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... samples were weighed and homogenized in DMEM containing 10% FBS and 1% antibiotics using 5 mm stainless steel beads (Qiagen) and the TissueLyser II (Qiagen) ...
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bioRxiv - Bioengineering 2021Quote: ... and 10 mM imidazole was added to the supernatant then loaded to a gravity column packed with Ni-NTA resins (Qiagen) pre-equilibrated with 20 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Cells were then centrifuged at 300 x g for 10 minutes and vigorously resuspended in 350µL RLT Buffer (Qiagen, Germany) by vortexing for 30 seconds ...