Labshake search
Citations for Qiagen :
1551 - 1600 of 1739 citations for Genz 644282 CAS 529488 28 6 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from 200 ul of oral swab material using the Qiagen EZ1 DSP Virus kit on the automated EZ1 XL Advance instrument (Qiagen, Valencia, CA). Real-time quantitative reverse transcription – polymerase chain reactions (RT-qPCR ...
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bioRxiv - Genomics 2020Quote: The RNA was extracted from the three agroinfiltrated fruits of and control fruit for RNA isolation whole fruit were taken after peeling the RNA was extracted with the help of Qiagen RNeasy Plant Mini Kit (Qiagen, Valencia, CA) with precisely following manufactures instruction protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... and unfragmented genomic DNA (A260/A280 ≥ 1.8 and A260/A230 ≥ 1.9) was extracted from whole blood obtained from the subject and his parents using the Puregene Blood kit from Qiagen (Valencia, CA). Whole exome sequencing was performed using the service provided by Beijing Genomics Institute (Cambridge ...
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bioRxiv - Cancer Biology 2021Quote: RNA was isolated from HFC (n = 3) and LFC (n = 4) tumor samples using the RNeasy Plus Universal Mini Kit (QIAGEN, Valencia, CA) and RNA quality was confirmed using an Advanced Analytical Fragment Analyzer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The upper phase was then transfer to RNeasy Mini spin column and processed with Qiagen RNeasy Mini Prep Kit (Qiagen, Valencia, CA) and DNAse purification using the Turbo DNA-free kit (Life Technologies ...
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bioRxiv - Evolutionary Biology 2020Quote: The DNA of all 52 samples was extracted from silica-gel dried leaves using the Qiagen DNeasy Plant Mini Kit following slightly modified manufacturer′s instructions (Qiagen, Valencia, CA). DNA quality and concentration were verified and quantified using NanoDrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... with a scaled down version of the manufacturer’s protocol with Dnase treatment of RNA using either the kit’s DNase or the Qiagen RNase-Free DNase Set (Qiagen, Valencia, CA, USA). Water controls were performed with each extraction batch ...
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bioRxiv - Genomics 2021Quote: ... normal-molecular-weight (NMW) gDNA was extracted from the same samples using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... region was amplified using fungal-specific primers (60): ITS1F (5’-CTTGGTCATTTAGAGGAAGTAA-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’) and the HotStarTaq Plus Master Kit (Qiagen, Valencia, CA). Amplicons from different samples were pooled to equimolar concentrations and purified of short fragments using Agencourt Ampure beads (Agencourt Bioscience Corporation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA and DNA were extracted from fecal and urine samples using the QIAamp MinElute Virus Spin Kit (Qiagen, Valencia, CA, USA), but using 20μg of linear polyacrylamide (VWR ...
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bioRxiv - Immunology 2020Quote: Dissected specific areas from each three MFPE sections were measured and introduced into RNase-free 0.5 ml Eppendorf tubes with buffer PKD from the miRNeasy FFPE Kit (Qiagen, Valencia, CA, USA). Direct microdissection of IHC stained MFPE sections were attempted but failed to provide RNA with enough quality and yields for further microfluidic quantitative PCR assays.
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bioRxiv - Immunology 2020Quote: Dissected specific areas from each three MFPE sections were measured and introduced into RNase-free 0.5 ml Eppendorf tubes with buffer PKD from the miRNeasy FFPE Kit (Qiagen, Valencia, CA, USA). Direct microdissection of IHC stained MFPE sections were attempted but failed to provide RNA with enough quality and yields for further microfluidic quantitative PCR assays.
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was isolated from 50 to 300 µl nematode pellets using the Blood and Tissue DNA isolation kit (cat# 69506, QIAGEN, Valencia, CA) following established protocols (Cook et al ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was isolated from 100 to 300 µl nematode pellets using the Blood and Tissue DNA isolation kit (cat# 69506, QIAGEN, Valencia, CA) following established protocols (Cook et al ...
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bioRxiv - Genomics 2020Quote: ... we extracted genomic DNA from leaf segments cut from areas with one pustule or very few pustules using the DNeasy Plant Mini Kit (Qiagen, Valencia, CA). We then amplified the ITS2 region using the primer pair RUST2inv (5′-GATGAAGAACACAGTGAAA ...
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bioRxiv - Genomics 2021Quote: A half-loopful overnight grown bacterial culture was used to extract the genomic DNA using the QIAGEN Genomic-tip 100/G (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and the cDNA was loaded onto the “Mouse Toll-Like Receptor Signaling Pathway” RT2 Profiler PCR Array according to the manufacturer instructions (Qiagen, Valencia, CA). Expression of the target genes were normalized to the geometric mean of Ct values of the two housekeeping genes Gusb and Hsp90a using the ddCt method (n = 3) ...
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bioRxiv - Genetics 2022Quote: ... We extracted genomic DNA from wing biopsies of live-captured individuals using the DNeasy Blood and Tissue protocol (Qiagen, Valencia, CA, USA). DNA from museum samples was extracted using previously described phenol-chloroform methods (56) ...
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bioRxiv - Evolutionary Biology 2022Quote: DNA was extracted from tail clips from offspring and parents using the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA, USA), and quantified using the Qubit fluorometer broad range kit (Thermofisher ...
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bioRxiv - Microbiology 2022Quote: Viral RNA was extracted from 200 ul of nasopharyngeal swab and BAL material using the Qiagen EZ1 DSP Virus kit on the automated EZ1 XL Advance instrument (Qiagen, Valencia, CA). Real-time quantitative reverse transcription – polymerase chain reactions (RT-qPCR ...
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bioRxiv - Physiology 2022Quote: ... Genomic DNAs of 61 weaned pups (2 pups died before weaning) were collected from their tails with a DNeasy Tissue kit (Qiagen, Valencia, CA). Six Ptger3-tTA BAC transgenic founder rats were identified by PCR for the presence of the tTAad-BGH insert ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from stock virus in allantoic fluid using the QIAamp Viral RNA Mini Kit (Qiagen Inc., Valencia, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from purified CD4+ TN and TCM cells and from 24ST1NLESG cells using the RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 2 g of soil was immediately transferred into pre-weighed sterile tubes containing 3 ml of LifeGuard soil preservation solution (Qiagen, Toronto, CA) to stabilize the RNA ...
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bioRxiv - Microbiology 2022Quote: ... and the sediments were used to coextract the total RNA and DNA using the RNeasy PowerSoil Total RNA isolation kit and the DNA elution kit according to the manufacturer’s protocol (Qiagen®, Valencia, CA). To eliminate potential contaminant DNA in RNA samples ...
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bioRxiv - Physiology 2022Quote: ... total DNA was extracted from approximately 15 mg of muscle tissue and 300 mg of bone marrow using the QiAmp DNA mini kit (Qiagen, Valencia, CA). In subsequent experiments ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Total mRNA was extracted from lung tissue kept in RNAlater stored at -20 °C using a RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Each pulverized sample was resuspended in 350uls of Qiagen RNeasy Plus Mini RTL lysis buffer and RNA extracted as per manufacturer recommendations (Qiagen, Valencia, CA). The Lab Chip GX (Perkin Elmer ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from triplicates of 20 freshly dissected guts of 20-day old WT and CG3740/nazo mutant males using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA) per manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... DNA was precipitated with 100% ethanol and 3 M sodium acetate at -80°C for 60 minutes and then cleaned using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Valencia, CA) per manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from approximately 100 mg of frozen stem tissues for each sample using the Qiagen RNeasy Plant Mini-Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: DNA from insect samples and plants was isolated using the DNeasy Blood and Tissue or DNeasy plant kits (Qiagen, Valencia, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The gene network analysis for the selected 10 marker genes was conducted using Ingenuity Pathway Analysis Software (Version 01-22-01; Ingenuity Systems, Qiagen, CA, USA). This analysis aligned the marker genes with established genetic pathways and networks ...
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bioRxiv - Molecular Biology 2024Quote: ... Total DNA was isolated from the dried feces (one fecal sample was ca. 60 mg) using a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and purified using a Geneclean Spin Kit (MP-Biomedicals ...
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bioRxiv - Cancer Biology 2024Quote: ... Targeted knockdown was conducted with 100nM ON-TARGETplus smartpool human c-MYC (mixture of 4 individual siRNA) and/or human KLF6-SV1 siRNA (Dharmacon, Lafayette, CO) with HiPerfect (Qiagen, Valencia, CA).
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from HSMCs that were untreated or treated with various stimuli using the RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... All nucleic acid isolation from tissue sections was performed using a QIAcube automated sample preparation system according to the manufacturer’s instructions (Qiagen Inc., Valencia, CA). Sections were processed in random batches of 12 on the QIAcube to avoid confounding section number with potential technical sources of variation associated with nucleic acid isolation.
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bioRxiv - Genomics 2024Quote: The DNA of the 80 used for GBS was isolated using the spin-column DNeasy plant mini kit (Qiagen USA, Valencia, CA) (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... The SARS-CoV-2 N gene-specific primers were used to amplify a 97 bp product by conventional PCR and this was purified by the Qiagen gel extraction kit (Qiagen, CA, USA). The purified N gene PCR products were used in real-time PCR to prepare a standard curve ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions for all samples were pooled and purified by agarose gel electrophoresis and gel extracted using the QIAquick kit (Qiagen, Valencia, CA). Sequencing of the final libraries was performed on the Illumina MiSeq instrument using 600-cycle kits by the CCHMC DNA Core.
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bioRxiv - Immunology 2023Quote: Fecal pellets were collected from female mice in each housing cohort and genomic DNA (gDNA) was extracted with the Qiagen DNAeasy extraction kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The upper phase was then transferred to RNeasy Mini spin column and processed with a Qiagen RNeasy Mini Prep Kit (Qiagen, Valencia, CA), before DNAse purification using the Turbo DNA-free kit (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: Rodent tissue and cell culture supernatant were used for viral RNA extraction using the QIaAmp RNA viral extraction kit (Qiagen, Valencia, CA) and tested for alphaviruses using reverse transcription-polymerase chain reaction (RT-PCR ...
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bioRxiv - Genetics 2023Quote: DNA was extracted from blood samples collected with breeders’ written permission at the annual Utah Premier Pigeon Show or from our lab colony using the Qiagen DNEasy Blood and Tissue Kit (Qiagen, Valencia, CA). Samples were treated with RNAse during extraction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... EDTA blood or tissue samples was extracted using DNeasy® DNA kits following the manufacturer’s protocol (Qiagen Inc., Valencia, CA, USA, 2014). A fragment of the mtDNA control region was amplified using the mt15996L (5’-TCCACCATCAGCACCCAAAGC-3’ ...
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bioRxiv - Genetics 2023Quote: ... Total DNA extraction of algal biomass and supernatants (i.e. filters) was performed in sterile conditions (under laminar flow hood) using DNeasy PowerSoil™ Kit (Qiagen Laboratories, CA). For Ostreobium biomass ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was extracted from the whole body of specimens using a Qiagen DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA, USA), following the manufacturer’s protocol with slight modifications to increase DNA yield from insects ...
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bioRxiv - Cancer Biology 2023Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... TRIZOL/cell mixture was next transferred into a clean microcentrifuge tube and total RNA was purified using RNeasy Mini Kit (QIAGEN, Valencia, CA) and quantified by spectrophotometer (NanoDrop) ...
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bioRxiv - Evolutionary Biology 2023Quote: Dried leaf material from each plant was ground into powder form using a Qiagen TissueLyser and DNA was extracted using Qiagen DNeasy Plant kits (Qiagen, Valencia CA). Reduced representation libraries were prepared using a double-digest restriction-site associated DNA sequencing (ddRADseq ...