Labshake search
Citations for Qiagen :
1501 - 1550 of 1739 citations for Genz 644282 CAS 529488 28 6 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... The reaction data were registered and analyzed using the Rotor-Gene 6000 series and software 1.7 (Corbett Research, Qiagen Inc., Valencia, CA).
-
bioRxiv - Molecular Biology 2020Quote: ... The crosslinking of eluted immunocomplexes was reversed and the recovered DNA was recovered using a QIAGEN QIAquick PCR purification kit (Qiagen, Valencia, CA). ChIP-seq library was prepared using TruSeq ChIP Library Preparation Kit (IP-202-1024 ...
-
bioRxiv - Cell Biology 2022Quote: ... DNA samples were extracted from PKS skin fibroblasts and normal control skin fibroblasts using the Puregene DNA isolation kit (Qiagen, Valencia, CA) with modified protocol ...
-
bioRxiv - Microbiology 2020Quote: ... RNA from cell lysates or cell culture supernatants was extracted using the Qiagen QIAamp viral RNA mini kit (Qiagen, Valencia, CA, USA). Reverse transcription (RT ...
-
bioRxiv - Cancer Biology 2020Quote: ... pancreatic total RNA or isolated pancreatic ductal cells was isolated using Trizol and further purified with an RNeasy Mini Kit (Qiagen, Valencia, CA). Reverse transcription was performed with the Superscript III RT-PCR Kit (Invitrogen) ...
-
bioRxiv - Genomics 2021Quote: ... Total genomic DNA was extracted from 5-day-old seedlings germinated from five well-developed seeds of each accession using a Qiagen DNeasy plant mini kit (Qiagen, CA, USA) following the protocol provided by the manufacturer ...
-
bioRxiv - Immunology 2021Quote: ... Plates were spun down: supernatants were used to measure production of immune mediators and cell pellets were resuspended in Qiazol (Qiagen, Valencia CA) for RNA extraction ...
-
bioRxiv - Microbiology 2021Quote: ... The aqueous phase was separated and mixed with an equal volume of 70% ethanol and immediately applied to an RNeasy Mini Spin Column (Qiagen, Valencia, CA). RNA was isolated according to the manufacturer’s protocol ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: DNA was extracted from blood or feather samples collected from 21 individuals in the field in 2012 using a QIAGEN DNEasy Blood and Tissue Kit (QIAGEN, Valencia, CA) following manufacturer’s protocols ...
-
bioRxiv - Neuroscience 2020Quote: ... along with the human NaV β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.X:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...
-
A New Gene Set Identifies Senescent Cells and Predicts Senescence-Associated Pathways Across TissuesbioRxiv - Cell Biology 2021Quote: ... tibiae were centrifuged as noted above to remove bone marrow elements and then were immediately homogenized in QIAzol Lysis Reagent (QIAGEN, Valencia, CA) and stored at −80°C ...
-
bioRxiv - Cell Biology 2021Quote: ... the cells were co-transfected with the 9 × GCCG-reporter construct and the renilla-luciferase control vector using SuperFect (Qiagen, Valencia, CA) for 24 h ...
-
bioRxiv - Plant Biology 2020Quote: ... RNA was isolated from frozen leaflet samples (100 mg of tissue) using the Qiagen RNeasy Plant Mini Kit (Qiagen, Valencia, CA, U.S.A) following manufacturer’s protocol ...
-
bioRxiv - Systems Biology 2021Quote: Approximately 10 mL of amniotic fluid from each animal was used for cell-free RNA extraction with the QIAamp Circulating Nucleic Acid Kit (QIAGEN, Valencia, CA). RNA from the lung and hippocampus was extracted with the RNeasy Universal Mini Kit (QIAGEN ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA from each butterfly was purified using Qiagen’s DNeasy Blood and Tissue kit in accordance with the manufacturer’s recommendations (Cat. No. 69581; Qiagen Inc., Valencia, CA, USA). We then created reduced complexity ...
-
bioRxiv - Genomics 2020Quote: ... and OA (from Wuxi Freshwater Fisheries Center in China) were extracted from muscle tissues using a Blood & Cell Culture DNA Midi Kit (Q13343, Qiagen, CA, USA). We also obtained genomic DNAs from ONJp with a YY genotype ...
-
bioRxiv - Neuroscience 2020Quote: ... One thousand ng of total RNA from each ARC-ME fragment were reverse transcribed (RT) using the Omni RT Kit (Qiagen, Valencia, CA) in the presence of random hexamer primers (Invitrogen ...
-
bioRxiv - Microbiology 2021Quote: DNA from plastics was extracted using a modified bead-beating approach combined with the Puregene Tissue DNA extraction kit (Qiagen, Valencia, CA) as previously described 69 ...
-
bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from the GCL tissue by the “cut and LPC” method using a PALM Zeiss UV laser capture microdissection system and an RNeasy kit (Qiagen, Valencia, CA), and a portion (1 ng ...
-
bioRxiv - Microbiology 2021Quote: ... All amplifications of the rps10 locus were carried out using the QIAGEN Type-it Mutation Detect PCR Kit (QIAGEN, 206343, Valencia, CA). Multiplex PCR reactions were performed in 35 µL with 14 ng template DNA and 1 X final buffer concentration ...
-
bioRxiv - Plant Biology 2020Quote: ... the total RNA from the cell pellet was extracted using RNeasy Plant Mini Kit according to manufacturer’s manual (Qiagen, Carlsbad, CA, USA). 1 μg of total RNA was used to synthesize cDNA using iScript™ cDNA Synthesis Kit (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted and purified as described in [9] using the Quick-RNA MiniPrep Kit (Zymoresearch, Irvine, CA, USA) and RNeasy MinElute Cleanup Kit (QIAGEN, Hilden, Germany). Per sample at least 150 ng of high-quality RNA were obtained ...
-
Cell-free DNA as a biomarker for prostate cancer: elevated concentration and decreased fragment sizebioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from 7 to 55 mL of plasma using the Qiagen QIAamp Circulating Nucleic Acid Kit (Qiagen, Redwood City, CA), and double eluted with 40 μL of Qiagen Elution Buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... total RNA was purified from frozen biopsies using the QiaSymphony robot in combination with the QiaSymphony RNA Mini kit (Qiagen, CA, USA) according to the Manufacturers protocol including DNase treatment ...
-
bioRxiv - Cell Biology 2021Quote: ... The linearized vector DNA of pPB-CAG-mTpd52 ORF-HA was purified using a QIAquick Gel extraction kit (Qiagen, Chatsworth, CA, USA) and adjusted to a final concentration of 3 ng/μl in Tris-EDTA buffer for pronucleus injection ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... We extracted high molecular weight genomic DNAs from the muscle tissues of a single individual (male Bj/Bf F1 offspring, Bb/Bf F1 offspring with unidentified sex) using the DNeasy Blood & Tissue Kit (QIAGEN, Valencia, CA), and inspected the DNA quality by Qubit 2.0 Fluorometer (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2022Quote: ... Gene identifiers as well as average Log2(Fold Change) were used as input for Ingenuity Pathway Analysis (IPA) (Qiagen, Redwood City CA) where IPA-defined core analyses for Canonical Pathways and Disease and Biological functions were performed using all primary cell subsets (Figure 1 ...
-
bioRxiv - Cancer Biology 2022Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...
-
bioRxiv - Neuroscience 2021Quote: DNA was isolated from tail biopsies using the Gentra Puregene Mouse Tail Kit according to the manufacturer’s instructions (Qiagen, Valenica, CA, USA). Genomic DNA was digested with BamHI-HF (New England Biolabs ...
-
Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Each primer (3.5 μl) was combined with reverse-transcriptase reagent (1 μl) and SYBR® Green master mix (25 μl) (Qiagen, Valencia, CA). The thermal profile was as follows ...
-
bioRxiv - Plant Biology 2021Quote: All reverse transcription (RT) reactions were performed using SuperScript II following the manufacturers’ instructions with 2.5 mM Random Hexamers (Qiagen; Valencia, CA, USA), 100 units SuperScript II and 30 units RNaseOUT (Invitrogen ...
-
bioRxiv - Microbiology 2021Quote: ... Total RNA was purified from 140 µL of each saliva sample using a QIAamp Viral RNA Mini Kit (Qiagen, Valencia, CA, USA), and eluted from the RNA binding columns using 80 µL of elution buffer ...
-
bioRxiv - Plant Biology 2021Quote: ... Young leaves from 20-day-old plants were collected for DNA extraction using the QIAGEN DNeasy Plant Mini Kit following the manufacturer’s instructions (QIAGEN, Valencia, CA, USA). DNA quality was determined via agarose gel electrophoresis and the quantity was measured on a NanoDrop (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... bearing a C-terminal histidine tag (ACRO Biosystems, Newark, NJ) was coated at 2 μg/ml on a Ni-NTA plate (Qiagen, Valencia, CA). After washing and blocking ...
-
bioRxiv - Immunology 2020Quote: RNA from B1a B cells was reverse transcribed and amplified using a Qiagen OneStep RT-PCR kit (Qiagen Inc., Valencia, CA, USA) and iRepertoire® mouse BCR heavy chain (MBHI ...
-
bioRxiv - Molecular Biology 2022Quote: We snap-froze tissue samples at the time of biopsy or necropsy and extracted DNA using the QIAamp DNA Mini Kit (Qiagen, Valencia, CA). We isolated DNase-treated total RNA from 100 mg of tissue ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Ligated DNA fragments ranging in size from 300 to 450 base pairs (bp) were extracted from 2% low-melt agarose gels and purified using a MinElute gel extraction kit (Qiagen, Valencia, CA). The recovered fragments were amplified using PCR ...
-
bioRxiv - Systems Biology 2020Quote: ... RNA and protein fractions using silica-membrane spin columns from the AllPrep DNA/RNA/Protein kit (cat# 80004, Qiagen, Chatsworth, CA, USA). PBMCs were processed in a randomized order ...
-
bioRxiv - Genetics 2021Quote: DNA was extracted from blood samples collected with breeders’ written permission at the annual Utah Premier Pigeon Show or from our lab colony using the Qiagen DNEasy Blood and Tissue Kit (Qiagen, Valencia, CA). Samples were treated with RNAse during extraction ...
-
bioRxiv - Plant Biology 2020Quote: ... and a total soil mass of no more than 250 mg was transferred from each tube into a sterile Qiagen PowerSoil (Qiagen, Valencia, CA) bead tube ...
-
bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from exponentially growing cells using spin columns and reagents in the RNeasy Midi Kit following methods provided by the company (Qiagen, Carlsbad, CA). RNA quality check ...
-
bioRxiv - Microbiology 2020Quote: ... Two PowerBead preparations of 0.5 g were used along with the PowerSoil Pro DNA Isolation kit (MoBio Labs/QIAGEN, Carlsbad, CA, USA) according to the instructions ...
-
bioRxiv - Genomics 2021Quote: ... Genomic DNA was extracted from the tongue tissue after treating the tissue with proteinase K using a Qiagen Genomic Tip Kit for High Molecular Weight DNA following Qiagen’s extraction protocol (Qiagen, Valencia, CA, USA). After extraction ...
-
bioRxiv - Genomics 2021Quote: ... The DNA extraction was carried out using Carlson lysis buffer by following Oxford Nanopore suggested protocol using Genomic tip 20 of Blood and cell culture kit (Qiagen, CA, USA) with few modifications ...
-
bioRxiv - Microbiology 2021Quote: ... Blood was obtained for lymphocyte preparation 3-5 days after the fifth immunization and RNA was prepared from lymphocytes using the RNeasy kit (Qiagen, Valencia, CA). A VHH-display phage library was prepared essentially as described previously [70] following each of the rounds of alpaca immunization ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were transiently co-transfected with NaV1.2 and the accessory β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.2:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...
-
bioRxiv - Physiology 2020Quote: The Fbxl22 proximal promoter region was amplified from genomic DNA isolated from C2C12 cells using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... The DNA was visualized under UV transillumination and the target bands excised and purified from the gel using QIAquick PCR purification kit (Qiagen, Valencia, CA) following manufacturer’s instructions and sequenced by Sanger method (Macrogen Europe ...
-
bioRxiv - Zoology 2021Quote: ... unfed and partially engorged tick tissues (salivary gland, midgut, ovary) from PA only with the DNeasy Blood and Tissue kit catalog # 69506 (Qiagen, Valencia, CA) using th standard protocol provided by the manufacturer ...
-
bioRxiv - Zoology 2021Quote: ... dead mites were pierced with an 18-gauge needle under a dissecting microscope and digested overnight in lysis buffer and proteinase K at 56 °C (Qiagen, Valencia, CA). Final DNA product from each mite was eluted in 60 µL of AE buffer ...