Labshake search
Citations for Qiagen :
1401 - 1450 of 1739 citations for Genz 644282 CAS 529488 28 6 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was isolated from cell lysate by phenol::chloroform extraction and spin-column (miRNAeasy kit, Qiagen, San Diego, CA, USA), quantified by spectrophotometry and assessed for quality using the Agilent 2100 bioanalyzer (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted genomic DNA from pools of 60 whole adult males and 15 whole virgin adult females using DNeasy Blood and Tissue kit (Qiagen, CA) and Promega DNA Clean and Prep Kit (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... Extracellular vesicle (EV)-bound total RNA was isolated from the vesicles using the exoRNeasy Serum/Plasma Midi Kit (Qiagen, Valencia, CA) by membrane affinity column purification per the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... approximately 300 mg dried tissue was subsampled into PowerBead tubes and DNA extractions were completed using the DNeasy Power Soil kit (Qiagen, CA) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: ... RNA was isolated from maternal and fetal tissues (Table 3 and 4) using QIAamp cador pathogen mini kit (Qiagen, Valencia, CA). ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... ~20 ng genomic DNA was amplified using a whole-genome amplification (WGA) kit according to the manufacturer’s instructions (Qiagen REPLI-g Mini Kit, Qiagen, Valencia, CA). The REPLI-g Kit is developed based on multiple displacement amplification (MDA) ...
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bioRxiv - Genomics 2021Quote: ... Total RNA was extracted from a pool of four 48 hour post-eclosion adult females using a Qiagen RNeasy Plus Mini kit (Qiagen, CA). RNA-seq libraries were prepared with 2μg of total RNA using the Illumina Stranded mRNA library prep kit and were sequenced on a HiSeq2500 instrument following standard Illumina protocols ...
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bioRxiv - Physiology 2021Quote: ... Crtc2 and GFP expression plasmids were purified from DH5a Escherichia coli cultures using an EndoFree plasmid kit (Qiagen, Valencia, CA, USA), and resuspended in 71 mM sterile PBS ...
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bioRxiv - Plant Biology 2021Quote: ... from non-inoculated and inoculated seedlings at 72 hours post inoculation (hpi) and total RNA was extracted using the RNeasy Mini kit (Qiagen, CA). The RNAseq library was constructed using TruSeq RNA library prep kit v2 (Illumina ...
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bioRxiv - Biochemistry 2020Quote: ... Canonical pathways associated with differentially expressed proteins were identified by Ingenuity Pathway Analysis (IPA, Ingenuity Systems, Qiagen, Redwood, CA, USA; www.ingenuity.com). This program is based on an algorithm that uses curated connectivity information from the literature to determine the network of interactions among the differentially expressed proteins and canonical pathways in which they are involved (Calvano et al. ...
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bioRxiv - Microbiology 2021Quote: Samples were homogenized and nucleic acids were extracted using the MoBio RNA PowerSoil® Total RNA Isolation Kit (Qiagen, CA, USA) and the MoBio RNA PowerSoil® DNA Elution Accessory Kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was isolated from Hela cells harvested from confluent cultures according to the manufacturer’s instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA). RNA was used as a template for reverse-transcriptase polymerase chain reaction (RT-PCR ...
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bioRxiv - Immunology 2022Quote: ... The lysate was then clarified by centrifugation at 16,000 x g for 45 minutes and then applied to 3 mL Ni-NTA resin (Qiagen, Valencia, CA) equilibrated with buffer A ...
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bioRxiv - Pathology 2022Quote: ... RNA concentrations were determined spectrophotometrically and 1µg of total RNA was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen, Valencia, CA). Real-time RT-PCR was performed using a StepOne real time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was extracted from leaf midribs and petioles of each tree using the Plant DNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: We extracted the DNA from the filters at UCLA using the Qiagen DNEasy Blood and Tissue kit (Qiagen, Valencia, CA, USA). To maximize eDNA recovery ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from cells with a combination of TriZol and RNeasy Mini RNA isolation kit (QIAGEN, Valencia, CA, USA), and reverse-transcribed using high capacity cDNA transcription kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... The high-quality reads were de novo assembled using the short reads assembly program Trinity version 2.3.2ref56 as well as a CLC Genomics Workbench version 10 (Qiagen, Carlsbad, CA, USA). The Trinity assembly yielded a total of 74,151 predicted ‘genes’ with their isoforms totaling 171,567 predicted ‘transcripts’ (i.e. ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from cultures grown in their preferred conditions using the Gentra Puregene Tissue Kit (Qiagen, Valencia, CA, USA). Samples were sequenced using the MiSeq 2×250 bp and HiSeq 2×150 bp paired-end read technology (Illumina ...
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bioRxiv - Physiology 2021Quote: Approximately 100 ng/µL of total DNA was extracted from 20 mg tissue (QiAMP DNA mini kit Qiagen, Valencia, CA, USA). DNA from bone marrow was extracted from the bone marrow suspension and centrifuged for 10 min at 2000 g ...
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bioRxiv - Neuroscience 2021Quote: RNA samples from WT and Rlip+/- mouse liver and brain were prepared using the RNeasy mini kit from Qiagen (Valencia, CA) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected with 200 ng total endotoxin-free plasmid DNA using the Effectene transfection protocol (Qiagen Inc., Valencia, CA, USA). Plasmid DNA encoded for C-terminally EGFP- or DsRed-monomer tagged Panx1b fusion proteins ...
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bioRxiv - Microbiology 2021Quote: DNA was isolated from Peripheral blood mononuclear cells (PBMCs) using a DNeasy blood and tissue extraction kit (Qiagen, Inc, Valencia, CA). DNA concentration was determined using a NanoDrop-1000 Spectrophometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The histidine-tagged PFam54-IV were produced and purified by nickel affinity chromatography with Ni-NTA agarose according to the manufacturer’s instructions (Qiagen, Valencia, CA).
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bioRxiv - Developmental Biology 2022Quote: ... Differentially expressed genes were identified using the Proportional Estimation test based on Kal’s test feature in CLCGWB (Qiagen, Redwood City, CA) using the raw read counts for our bulk RNAseq datasets ...
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bioRxiv - Microbiology 2022Quote: ... with 1 min rest on ice in between each round of bashing) on a TissueLyser II beadmill (Qiagen, Valencia, CA, USA), then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: ... Tissue was flash frozen in liquid nitrogen and DNA was isolated using a Qiagen Plant DNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and the RNA was extracted using the Qiagen RNEasy RNA mini prep kit columns as per manufacturer’s instructions (Qiagen, Valencia, CA). Resulting RNA concentration and quality was determined using the NanoDrop ND-1000 ...
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bioRxiv - Zoology 2020Quote: ... Samples were extracted in a room dedicated for this purpose using the DNeasy Blood & Tissue Kit (Qiagen, Inc. Valencia, CA, USA) with a modified protocol described in Carim et al ...
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bioRxiv - Biophysics 2020Quote: ... HEK-hP1cells were co-transfected with 0.2 μg plasmid DNA of paxillin-mApple (generous gift from Michael Davidson) using transfection reagent Effectene (Qiagen, Valencia, CA), and were cultured for 24 hours.
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bioRxiv - Developmental Biology 2020Quote: ... From old liver tissues and clotted blood samples DNA was extracted manually using QIAamp DNA Blood Midi Kit and the DNeasy Tissue Kit according to manufacturer’s protocol (Qiagen, Valencia, CA). DNA from BA10 was extracted on an automated nucleic acid extraction platform Anaprep (Biochain ...
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bioRxiv - Physiology 2020Quote: Total RNA was isolated from liver of WT and SOD1G93A mice using RNeasy Lipid Tissue extraction kit according to manufacturer’s instructions (QIAGEN, CA, USA). The total RNA was purified from genomic DNA contamination then converted to cDNA using iScriptTM gDNA Clear cDNA synthesis kit according to manufacturer’s instructions (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: Total RNA was extracted from the gill and liver tissues using a Qiagen RNeasy Plus Mini Kit (Qiagen, Toronto, ON, CA) following manufacturer’s protocols ...
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bioRxiv - Evolutionary Biology 2020Quote: DNA was extracted from lesions present on each herbarium voucher using either a Qiagen DNEasy Plant Mini Kit (Qiagen, Valencia, CA) or a modified CTAB method using DNEasy Plant Mini Kit spin columns for cleaning and purifying DNA12 ...
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bioRxiv - Neuroscience 2021Quote: ... Total cellular RNA was extracted from amygdala and NAc punches using Qiazol reagent and the RNAeasy Midi kit (QIAGEN, Valencia, CA). Total RNA was reverse transcribed into cDNA using VILO master mix (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... From tissues and clotted blood samples DNA was extracted manually using QiaAmp DNA Blood Midi Kit and the DNeasy Tissue Kit according to manufacturer’s protocol (Qiagen, Valencia, CA). DNA from BA10 was extracted on an automated nucleic acid extraction platform Anaprep (Biochain ...
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bioRxiv - Biochemistry 2020Quote: ... The primers used for pyrosequencing were designed based on a previous study25 using PyroMark Assay Design 2.0 (Qiagen, Valencia, CA, USA).
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bioRxiv - Systems Biology 2020Quote: ... and DNA extraction was performed on a consistent total biomass quantity for all samples using DNeasy mini kit with QIAcube (Qiagen, CA). Extracted DNA and RNA in each sample were quantified using dsDNA HS assay kit and RNA HS assay kit on the Qubit 2.0 Fluorimeter (Life Technologies ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA was extracted from leg and/or thoracic muscle tissue using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was monitored on Rotor-Gene® Q-Pure Detection System (Software Ver. 2, Qiagen Inc., Valencia, CA, USA) and performed using QuantiFast SYBR® Green PCR Master Mix (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was precipitated in isopropanol and then subjected to additional purification using the RNeasy RNA isolation kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... total genomic DNA of FAFU-HL-1 was extracted using a Qiagen DNeasy Plant Mini kit following the manufacturer’s instructions (Qiagen, Valencia, CA). For ONT sequencing ...
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bioRxiv - Genomics 2020Quote: Total genomic DNA for all 38 samples (Table S1) was extracted with the Qiagen DNeasy Plant Mini Kit (Qiagen, Valencia, CA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: DNA was isolated from P14 tail biopsies using the Gentra Puregene Mouse Tail Kit according to the manufacturer’s instructions (Qiagen, Valencia, CA). For Gabra2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The first replicate was extracted using the ISOLATE II Genomic DNA Kit as described in 2.1 and the second using the DNeasy Blood and Tissue Kit protocol (Qiagen, Valencia, CA) according to the manufacturer’s guidelines ...
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bioRxiv - Genomics 2020Quote: ... Two biopsy cores were used for routine formalin fixation for clinical diagnosis and the remaining four cores were preserved in PAXgene Tissue containers (Qiagen, CA) for subsequent genomic material extraction ...
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bioRxiv - Biochemistry 2022Quote: ... RNA was extracted from queen and worker heads using the RNeasy mini kit according to the manufactural instructions (Qiagen, Valencia, CA). Queens’ RNA was extracted from individual samples whereas heads of workers from the same cage were pooled together ...
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bioRxiv - Cancer Biology 2022Quote: ... Variants in variant call format files were evaluated for pathogenicity using Ingenuity Variant Analysis (IVA) version 4 (Qiagen Inc, Alameda, CA) and American College of Medical Genetics and Genomics (ACMGG ...
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bioRxiv - Genomics 2022Quote: We extracted genomic DNA from individual wild-caught adult Aedes in Colombia using Genetra Puregene Tissue Kits (Qiagen, Valencia, CA, USA), constructed barcoded libraries for sequencing using KAPA HyperPrep kits (Roche Sequencing ...
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bioRxiv - Neuroscience 2022Quote: RNA from the tissue punches was extracted and purified using RNeasy Lipid tissue mini kit (Qiagen, Inc., Redwood City, CA, USA) with QIAzol Lysis reagent (Qiagen ...