Labshake search
Citations for Qiagen :
1301 - 1350 of 1739 citations for Genz 644282 CAS 529488 28 6 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Total RNA was isolated from collected BmCPV-infected midguts with RNeasy R Plus Mini Kits (Qiagen, Valencia, CA, USA). After genomic DNA was digested by RNase-free DNase (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... The aqueous phase was removed and transferred to MaxTract High Density 2 mL tubes (Qiagen Inc, Valencia, CA, USA). Samples were then extracted a second time as described above and the aqueous phase from the repeated extractions for each sample were combined ...
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bioRxiv - Plant Biology 2023Quote: ... total RNA was isolated from liquid nitrogen ground whole etiolated seedlings using the RNeasy Plant Kit (Qiagen, CA, USA). cDNA libraries were constructed using the Illumina TruSeq Total RNA Sample Prep Kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from both NHP and human stool samples using the PowerFecal DNA Isolation Kit (Qiagen, Valencia, CA). Sequencing of the 16s small subunit ribosomal ribonucleic acid (SSU rRNA ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was retro-transcribed using a cDNA synthesis kit (Bio-Rad, Hercules, CA, USA or Qiagen, Hilden, Germany) and transcript levels were determined by RT-qPCR using the SsoAdvanced SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... cfDNA was extracted from 0.5 ml of plasma or serum using the QIAmp DNA Mini Blood kit (Qiagen, CA), according to the ‘‘Blood and body fluid protocol’’ and our own detailed protocol [37] ...
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bioRxiv - Molecular Biology 2024Quote: ... we sequenced the rbcL F3R3 region of 104 predominant plant species at the study site using the following methods. Total DNA was extracted from plant specimens (ca. 0.8 cm2) using a DNeasy Plant Mini Kit (Qiagen) and purified using a Geneclean Spin Kit (MP-Biomedicals) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by cell lysis and purification using immobilized metal-affinity chromatography (IMAC) in a Ni–NTA (Qiagen, Valencia, CA) column in binding buffer composed of 20 mM HEPES pH 7.25 ...
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bioRxiv - Immunology 2024Quote: ... DNA from the blood of Plasmodium chabaudi infected mice was isolated from QIAmp DNA blood kit (QIAGEN, Valencia, CA) as per the manufacturer instruction ...
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bioRxiv - Genomics 2024Quote: ... Homogenization buffer for RNA purification was made by adding 1:100 beta-mercaptoethanol to Buffer RLT (Qiagen, Valencia, CA) and kept on ice until use ...
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bioRxiv - Genomics 2023Quote: ... We extracted total RNA from most adult tissues (minus the brain) using the RNeasy Kit (QIAGEN, Redwood City, CA) and submitted the extracted total RNA to Novogene Ltd ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from the lungs using TRIzol reagent and purified by RNeasy mini columns (Qiagen, CA, USA). The eluted RNA was subjected to complementary DNA synthesis using a High-Capacity cDNA Reverse Transcription Kit as per the suggested protocol (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Viral genomic DNA was purified from the harvested cells using a QIAamp DNA Blood mini kit (Qiagen, CA, USA). The viral genome copy number was quantified by qPCR and normalized to the amount of total DNA.
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bioRxiv - Microbiology 2022Quote: ... adaptor trimming and de novo transcriptome assembly were carried out using CLC Genomics Workbench (Qiagen, Redwood City, CA, USA). A reference transcriptome was generated by a combined assembly of both hindgut wall and pocket reads using CLC Genomics Workbench v10.1 software with standard settings and an additional CLC-based assembly with the following parameters ...
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bioRxiv - Genetics 2023Quote: RNA from the collar cells of regenerating feather buds was isolated using the Qiagen RNEasy Kit (Qiagen, Valencia CA), treated with the Zymo PCR Inhibitor Removal kit (Zymo Research ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA treated with RNase-free DNase was cleaned up using the RNeasy Mini kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we extracted DNA from whole individuals (after gut removal) using a Qiagen DNEasy tissue kit (Qiagen, Valencia, CA, USA). We digested 1000 ng of DNA with the restriction enzymes EcoRI and NlaIII ...
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bioRxiv - Genomics 2023Quote: Genomic DNA was isolated from fresh leaves of selected germplasm panel using DNeasy 96 Plant Kit (QIAGEN, Valencia, CA), followed by genotyping with Illumina Golden Gate SNP assay ...
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bioRxiv - Immunology 2023Quote: ... Illumina adapters were completed by a second PCR carried out with the Qiagen Multiplex PCR kit (Qiagen, Valencia, CA), using 1 μl of the first PCR product as the template in a 20 μl reaction with the following program ...
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bioRxiv - Bioengineering 2023Quote: ... total RNA was extracted from about 1 × 106 cells from all groups using RNeasy Mini Kit (Qiagen, Valencia, CA), then using the random hexamer primer and M-MuLV reverse transcriptase kit (Fermentas ...
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bioRxiv - Microbiology 2023Quote: ... DNA from approximately 1g of surface sterilized seeds were isolated using DNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA) with necessary modifications ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was obtained from 5ml of stationary phase culture of parasites using the DNeasy Mini Kit (Qiagen, CA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from the feces samples collected on the filter using the DNeasy PowerSoil Kit (Qiagen, Valencia CA). A quarter of the filter paper was used for the extractions ...
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bioRxiv - Plant Biology 2024Quote: ... genomic DNA was extracted from mycelial mats of three isolates using DNeasy Plant Pro Kit (Qiagen Inc., Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 0.5% SDS) and frozen at −80°C until extraction using the QIAamp DNA Mini Kit (Qiagen Inc., Chatsworth, CA) according to the provided protocol.
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bioRxiv - Microbiology 2024Quote: Purified bacterial colonies were used for genomic DNA extraction using the QIAGEN Genomic-tip 100/G (Qiagen, Valencia, CA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from cervicovaginal swabs stored at −80 ℃ using the AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA). Before extraction ...
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bioRxiv - Microbiology 2024Quote: ... The aqueous phase was removed and transferred to MaxTract High Density 2 mL tubes (Qiagen Inc, Valencia, CA, USA). Samples were then extracted a second time as described above and the aqueous phase from technical replicates for each soil sample were combined ...
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bioRxiv - Microbiology 2024Quote: Separation of DNA and RNA was completed using the AllPrep DNA/RNA Mini Kit (Qiagen Inc, Valencia, CA, USA). The amount of DNA or RNA extracted was quantified using the Qubit 1X dsDNA Broad Range Kit or Qubit RNA High Sensitivity Kit ...
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bioRxiv - Biochemistry 2024Quote: ... The transcriptomes were assembled using Trinity scripts and analyzed using CLC Genomic Workbench 20.0.4 (Qiagen, Redwood City, CA, USA).
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bioRxiv - Evolutionary Biology 2024Quote: ... Total genomic DNA of the remaining abdominal content was extracted using the DNeasy blood and tissue kit (Qiagen, CA), following a modified version of the manufacturer’s protocol (supplementary material) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA from all other samples was extracted using the DNeasy Blood and Tissue Kit (Qiagen Inc., Alameda, CA, USA).
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bioRxiv - Developmental Biology 2020Quote: ... The PCR products were detected by gel electrophoresis and purified with the MinElute PCR Purification Kit (Qiagen, Valencia, CA, USA) and sequenced.
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bioRxiv - Genomics 2020Quote: WGA of single cells was performed by multiple displacement amplification using the REPLI-g Single Cell kit (Qiagen, Valencia, CA) as per the manufacturer’s instructions but at one quarter of the recommended volumes ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was extracted in our laboratory from each sample either using Qiagen DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA) or by salting out [35] and the DNA extract was quantified using Qubit dsDNA HS assay (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: RNA and protein were extracted from cell lines using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA, USA). Total RNA was also DNase treated using the TURBO DNA-free Kit (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from PAXgene Blood RNA tubes using the Qiagen PreAnalytiX PAXgene Blood miRNA Kit (Qiagen, Valencia, CA). The extraction protocol was performed either manually or with the Qiagen QIAcube extraction robot according to the company’s standard operating procedure.
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bioRxiv - Cell Biology 2021Quote: ... the cells were co-transfected with the reporter construct and the renilla-luciferase control vector using SuperFect (Qiagen, Valencia, CA) for 24 hr ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from ∼100 mg of cecal contents using the MoBio UltraClean Soil DNA extraction kit (Qiagen, Carlsbad, CA) following the manufacture’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... and plasmids containing the full-length reverse transcriptase gene were isolated with the QIAprep Spin Miniprep kit (Qiagen, Valencia, CA). A minimum of 20 clones from each sample were sequenced ...
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bioRxiv - Microbiology 2021Quote: ... total RNA from the individual sample was isolated and purified by using RNAse mini kit columns (Qiagen, Valencia, CA, USA) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... subsequently run on a 2% agarose gel and bands of the correct size (ca. 200 bp) were purified with the QIAquick Gel extraction Kit (Qiagen). From the columns ...
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bioRxiv - Molecular Biology 2020Quote: ... Cas3d and Cas10d proteins were purified from HEK293T cells expressing each His-tagged Cas protein using Ni-NTA agarose (Qiagen) and a gel filtration column (Superdex 200 increase 10/300 GL columns) ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from each fecal containing tube using the Tissue and Blood DNA Isolation kit (Qiagen, Valencia, CA) following the manufacturer’s instructions with slight modifications ...
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bioRxiv - Plant Biology 2021Quote: ... were set up with a CAS-1200N robotic liquid handling system (Corbett Research) and run for 50 cycles on a Rotor-Gene Q (Qiagen). Two technical replicates and three biological replicates were performed for each sample ...
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bioRxiv - Microbiology 2020Quote: ... MB897-1) were used to generate infectious virus by transfecting 293T cells using the Effectene transfection reagent (QIAGEN, Valencia, CA). Supernatant containing virus was collected 2 days post transfection ...
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bioRxiv - Microbiology 2020Quote: ... and a portion of the tissue (0.08-0.3g) was placed into pre-labeled microcentrifuge tubes containing 5 mm stainless steel beads (Qiagen Inc., CA). Lung samples were homogenized with DMEM + 2% FBS in a TissueLyser (Qiagen Inc. ...
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bioRxiv - Genomics 2022Quote: We extracted DNA from each individual’s blood sample using the DNeasy Blood and Tissue Kit (Qiagen, Inc., Valencia, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from sub-confluent plates using the RNeasy kit as directed by the manufacturer (Qiagen, Valencia, CA), as previously described (31) ...
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bioRxiv - Physiology 2020Quote: ... All the DEGs and heart abundant DEGs were further annotated by ingenuity pathway analysis (IPA; QIAGEN, Redwood City, CA, USA). Metabolic signaling pathway enrichment was done on the significantly DEGs by an adjusted p-value cutoff of 0.05 by IPA ...