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Citations for Qiagen :
1351 - 1400 of 10000+ citations for Mouse Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... Cells were treated with a pool of four small siRNAs per gene with the following sequences (Qiagen 1027280):
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bioRxiv - Physiology 2019Quote: The mRNA expression of 34 metabolic genes was analyzed using a custom RT2 Profiler PCR Array (Qiagen, USA). First ...
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bioRxiv - Immunology 2019Quote: ... Promoter-T3SS pairs were cloned upstream of the CatB gene and inserted separately into pQE30 (Qiagen, Hilden, Germany). Parallel constructs were made with CatB gene replaced by eGFP to produce expression plasmids used for imaging studies ...
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bioRxiv - Cell Biology 2019Quote: The absolute quantification by quantitative real-time PCR (qPCR) was performed with the Rotor-Gene Q thermocycler (Qiagen) and the QuantiNova SYBR Green PCR Kit ...
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bioRxiv - Immunology 2021Quote: ... using cycle threshold (Ct) values acquired from the amplification curve using the Rotor-Gene Q instrumentation software (Qiagen).
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bioRxiv - Immunology 2020Quote: ... Relative gene expression was calculated using the Qiagen webportal PCR array data analysis web-based software (Qiagen, Germany). Differential expression (up and down regulation ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a customized RT2 profiler array for selected 89 genes according to the manufacturer’s instructions (Qiagen) and run on QuantStudio 7 Flex (software v1.0) ...
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bioRxiv - Cancer Biology 2022Quote: ... Knockdown of EN2 gene was conducted by introducing four different siRNA primers into the cells (Qiagen, Hilden, Germany) (Table 2) ...
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bioRxiv - Cell Biology 2022Quote: ... Genes with significantly altered expression between PTPN23H/H and PTPN23+/+ IAT were analyzed with Ingenuity Pathway Analysis (Qiagen) to identify impacted pathways and candidate upstream drivers ...
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bioRxiv - Microbiology 2022Quote: ... one-step RT-qPCR was performed using SensiFAST™ SYBR® Lo-ROX One-Step Kit (Meridian Bioscience) with 2μL of RNA samples according to the manufacturer’s protocol on a Rotor-Gene Q apparatus (QIAGEN) using primers listed in Table 2 ...
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bioRxiv - Plant Biology 2024Quote: ... The library was checked with Qubit and real-time PCR (Rotor-Gene Q thermal cycler, Qiagen, Hilden, Germany) for quantification and bioanalyzer for size distribution detection ...
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bioRxiv - Neuroscience 2024Quote: ... Differentially expressed genes were identified using the bioconductor package limma (3.42.2) with FDR < 0.1 and the resulting gene lists were used as input for Ingenuity pathway analysis (Qiagen). Weighted gene co-expression network analysis was conducted using WGCNA R-package (ver 1.69) ...
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bioRxiv - Cell Biology 2024Quote: ... Gene expression changes associated with osteogenesis were assessed using an RT2 Profiler™ PCR Array (PAMM-026Z, Qiagen). A fold regulation threshold of 2 was used to determine up- or down-regulation of each gene in male and female PAE mice compared to control and data is presented as log2 fold change ...
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bioRxiv - Microbiology 2023Quote: ... The amplification reaction was conducted using a Rotor-Gene-Q PCR Cycler Real-Time PCR system (Qiagen, Australia). The cycling parameters were as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... ARPE-19 cells were transfected with a HIF-luciferase reporter gene construct (Cignal Lenti HIF Reporter, Qiagen, Netherlands). The HIF-luciferase construct encodes a firefly luciferase gene under the control of the hypoxia response element ...
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bioRxiv - Microbiology 2023Quote: ... The presence and nucleotide sequence of resistance genes were manually verified using the QIAGEN CLC genomics workbench 21.0.3 (QIAGEN Digital Insights ...
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bioRxiv - Microbiology 2024Quote: Full-length sdeC genes were amplified by PCR and cloned into the bacterial expression vector pQE-80L (Qiagen) with BamHI and SacI restriction sites to generate a 6xHis-SdeC-StrepII fusion construct (Supplemental Table S1) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 µM each of forward and reverse primers (listed in Supplemental information) on Rotor-Gene Q (QIAGEN). The thermal cycling conditions included an initial denaturation step at 95°C for 10 min ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from the indicated mouse tissues and cultured cells using RNeasy® Lipid Tissue Mini Kit (Qiagen, Valencia, CA, USA). Reverse transcription reactions containing 1 μg of total RNA were performed using PrimeScript™ (Takara ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from donor cecal contents and day 0 conventionalized mouse feces using the Qiagen PowerFecal DNA Kit (Qiagen, Hilden, Germany; 12830) following the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... eyes were enucleated and retinas were immediately dissected and total RNA was extracted from mouse retinal tissue using Qiagen RNeasy Mini Kit as per manufacturers protocol (Qiagen, Valencia, CA, USA) and reverse-transcribed using iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: RNA was extracted from mouse islets (∼300 islets per sample, pooled from ∼10 mice of the same genotype) using RNAeasy Micro Kit (Qiagen, Valencia CA, USA) as per manufactures instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from the mouse cells/tissues and human cell lines using the RNeasy® Mini Kit (Qiagen Inc., Germantown, MD). For human platelets and MEG-01 cells ...
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bioRxiv - Physiology 2020Quote: ... protein was extracted (Qiagen Tissue Lyser) from ∼50 mg frozen liver in tissue lysis buffer 80 ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL Protein Precipitation Solution (QIAGEN) was added ...
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bioRxiv - Microbiology 2023Quote: ... and The Protein Complex Suite (Qiagen). One microliter of 4.6 mg/ml protein sample suspended in crystallization buffer (25 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: Advanced Protein Purification buffer (APP; Qiagen): the APP buffer contains zinc chloride to precipitate protein at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from spiked blood (1×104 to 1 CFU/mL) using MolYsis Basic5 DNA extraction kit (Molzym, Bremen, Germany) combined with QIAamp UCP Pathogen Mini Kit (Qiagen, Venlo, Netherlands) following manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse tissue disruption and homogenization was performed in RIPA buffer supplemented with 1:100 protease using Tissue Lyser LT (Qiagen), for 5 min at 50 Hz ...
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bioRxiv - Cell Biology 2019Quote: The decellularized Balb/c mouse pancreas scaffolds were washed in PBS and homogenized-lysed for 1 hr in Tissue homozilyser II (Qiagen). The ECM proteins were dissolved in lysis buffer containing Tris HCl 0.06M ...
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bioRxiv - Biochemistry 2022Quote: ... we also probed for the Strep-tag on γTuNA (1:1000 dilution of Strep-tag mouse monoclonal antibody, cat. # 34850, Qiagen). Band intensities were measured in ImageJ and normalized in Prism 7 by the wildtype γTuNA band (positive control) ...
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bioRxiv - Biochemistry 2024Quote: ... co-expressing lambda protein phosphatase and purified with the QIAexpress Ni-NTA Protein Purification System (Qiagen, 30210) according to the manufacturers protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of purified RNA was reverse transcribed using RT2 First Strand Kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from 1×107 cells with the RNeasy® Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from MDM or THP-1 cells using RNAeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted using the RNeasy kit and treated with DNase 1(Qiagen). Three donor samples of human RPE/choroid were processed for RNAseq ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from 1-2 million cells using RNeasy Mini Kit (QIAGEN) using QIAcube (QIAGEN) ...
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bioRxiv - Microbiology 2019Quote: Total DNA was isolated from 1×108 cells using Puregene Core Kit A (Qiagen). Free minicircles and total kDNA content were analyzed as previously described (Bruhn ...
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bioRxiv - Cancer Biology 2019Quote: ... for 1 h at 37 °C and purified using QIAquick PCR Purification Kit (Qiagen). The pLVX-Puro lentiviral expression vector (Clontech ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted from 1 million mESCs using DNeasy Blood & Tissue Kit (Qiagen, 69506) and RNA was extracted from 450,000 HEK293T cells using the Direct-zol RNA MicroPrep Kit (Zymo ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from 1 ml of the slurry using a commercial kit (Qiagen).
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bioRxiv - Neuroscience 2021Quote: ... 1 μg of RNA was retrotranscribed in cDNA with QuantiTect Reverse Transcription Kit (Qiagen), followed by a PCR amplification of the subsequent transcripts ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was prepared from 1 μg RNA using a Quantitect Reverse Transcription kit (Qiagen) and diluted 1:20 in DEPC-treated water ...
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bioRxiv - Plant Biology 2021Quote: ... swc6-1 and pfd3,5 seedlings (three biological replicates) using RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed with Quantitect Reverse Transcription Kit (Qiagen) using oligo(dT) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of template DNA and the Qiagen PCR multiplex PCR kit (Qiagen, France) on a T100TM Thermal cycler (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: ... The product was purified from a 1% agarose gel (QIAquick Gel Extraction Kit, Qiagen).
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bioRxiv - Paleontology 2019Quote: ... plasmid minipreps were purified with a QIAprep Miniprep Kit (Qiagen, chimpanzee 1 and 2), and RBC Miniprep Kit (YPD100 ...
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bioRxiv - Neuroscience 2020Quote: ... cDNAs were synthesized from ∼1 µg total RNA using QuantiTect Reverse Transcription kit (QIAGEN) according to the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and stored in either 1 ml Cell Lysis Solution (Gentra Puregene Kit, Qiagen, USA), Queen’s buffer ...