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Citations for Qiagen :
1501 - 1550 of 10000+ citations for Mouse Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Illumina compatible libraries were generated using the Qiagen Q1ASeq 1-step amplicon kit (Qiagen, #180419), and sequencing was performed on the Illumina MiSeq platform using 2×250 paired end reads with a goal of generating 5,000 – 10,000 reads per sample ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from 1×106 MEL cells with the RNeasy Plus kit (Qiagen, 74134). RNA was reverse-transcribed using the Superscript III First-Strand Synthesis System (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Complementary DNA was synthesized from 1 μg of RNA using the QuantitTect RT kit (Qiagen). Quantitative PCRs (qPCR ...
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bioRxiv - Cell Biology 2023Quote: ... and total RNA (1 µg) was reverse transcribed using QuantiTect Reverse Transcription Kit (Qiagen, 205313) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... total genomic DNA was extracted from 1×106 parasites using DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... After chloroform: isoamyl alcohol (24:1) extraction followed by purification (Qiagen RNeasy Mini Kit, 74104), the biotinylated RNA was resuspended in water and biotinylated-RNA was isolated using Dynabeads MyOne Streptavidin C1 magnetic beads (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... and purified from 1% agarose gel using a QIAquick Gel Extraction Kit (Qiagen, Germantown, MD). A double-stranded insert was digested at the same restriction sites ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 RNA in plasma was extracted using the QIAamp Viral RNA Mini Kit (Qiagen). To amplify the 3’ half viral genome ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 hour and purified by agarose gel extraction (MinElute Gel Extraction Kit, Qiagen #28606). Plasmid constructs were created by Gibson cloning with NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621L ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 1 × 106 cells using the RNeasy Plus Mini Kit (Qiagen), and cDNA was synthesized with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: Total RNA was extracted from 1 – 2 x 108 cells using the RNeasy kit (Qiagen). DNA was removed using Turbo DNase (Applichem ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from ∼1 million cells using the RNeasy Mini Kit (Qiagen, #74104) with QIAshdredder Mini Spin Columns (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... 1 microgram of total RNA was reverse transcribed using the QuantiTect Reverse Transcription kit (Qiagen). Real-time RT-PCR was performed on 1:10 diluted cDNA using the SensiFAST SYBR No-ROX Kit (Bioline) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA samples were extracted from 1 million cells using the RNeasy Mini Kit (Qiagen, 74136) and treated with TURBO DNA-freeTM Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... QPCRs were run using the recommended protocol for 2x ProPlant SYBR Mix (Procomcure Biotech) on a Rotor-Gene Q (Qiagen). Technical triplicates were performed for each biological replicate ...
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bioRxiv - Developmental Biology 2020Quote: ... PMSG- or hCG-regulated genes in wildtype GCs that showed a differential expression in Erβnull GCs were selected as ERβ-regulated genes and subjected to Ingenuity Pathway Analysis (IPA; Qiagen Bioinformatics) to determine their involvement in folliculogenesis.
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bioRxiv - Neuroscience 2020Quote: ... The gene array reaction was performed using the RT2 Profiler™ Rat Synaptic Plasticity PCR Array (Cat. No. 330231, Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Significant probes were defined as p < 0.01 and resultant gene sets were uploaded to Ingenuity Pathway Analysis (IPA; Qiagen, Inc.) for probe set annotations and to query relationships between genes ...
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bioRxiv - Molecular Biology 2020Quote: Whole blood samples from 10 healthy volunteers (n = 5 females and n = 5 males) aged from 25 to 37 were collected into PAX gene RNA blood tubes (Qiagen). Total RNA samples were isolated using QIAcube automation with the PAXgene Blood miRNA Kit (Qiagen ...
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bioRxiv - Biophysics 2022Quote: ... Purified protein was diluted in PBS buffer (pH = 7.2) and the fluorescence intensity was recorded at 60 °C in the Rotor-Gene 6600 real-time PCR cycler (Qiagen) for 18 h ...
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bioRxiv - Immunology 2021Quote: ... PRRSV-2 RNA was used to amplify cDNA encoding the full-length of N gene by RT-PCR (One-step RT-PCR, QIAGEN) using specific primers with the forward containing a T7 promoter sequence at the 5’ end ...
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bioRxiv - Immunology 2019Quote: ... Gene set enrichment analysis was done using QIAGEN’s Ingenuity® Pathway Analysis software (IPA®, QIAGEN Redwood City,www.qiagen.com/ingenuity) on genes that passed nominal p<0.05 using “Canonical Pathways” and “Upstream Regulators” options ...
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bioRxiv - Neuroscience 2019Quote: ... Relative gene expression was measured using RT2 Profiler low-density PCR plates preloaded with qPCR primer assays for genes encoding GABA- and glutamate-related targets (PN: PARN-152ZA, Qiagen). Thermal cycling and data collection were accomplished using an ABI Real-Time PCR 7300.
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bioRxiv - Microbiology 2019Quote: ... Genes were predicted using Prokka (78) and plasmids were annotated and visualized via CLC Sequence Viewer (v8.0.0) (Qiagen, Aarhus, Denmark). For clade-to-clade chromosome comparisons ...
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bioRxiv - Microbiology 2020Quote: Selection of ARGs for the analysis was performed as follows: The Antibiotic Resistance Genes Microbial DNA qPCR arrays (Qiagen, USA) were used to screen for the presence of ARGs ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... The temperature was increased from 25 °C to 95 °C at 3 °C per minute and fluorescence was measured at 610 nm in a Rotor-Gene Q thermocycler (Qiagen). The experiment was performed in technical triplicates ...
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bioRxiv - Molecular Biology 2020Quote: ... Multiple sequence alignment and phylogenetic tree construction (using representative gene sequences) was performed using CLC sequence viewer (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2020Quote: ... and the cycle-threshold CT translated to bacterial load (estimated CFU per mL (eCFU/mL) using a standard curve on a Rotor gene Q 5plex platform (Qiagen). The cut-off for TB-MBLA positivity is a 30 CT value that corresponds to 1.0 log10 eCFU/mL ...
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Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: ... Thermal cycling was performed on the Rotor-Gene Q real-time PCR cycler and data were analyzed with the associated software (Qiagen) using a cycle threshold of 0.03 ...
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bioRxiv - Microbiology 2020Quote: ... The sequences were aligned against the PAO1 reference genome NC_002516 available in the “Pseudomonas Genome Database” (PseudoCAP) [37] and gene expression was quantified using the “CLC Genomics Workbench” software (QIAGEN). The numeric value of gene expression was normalized to Reads Per Kilobase of gene per million Mapped reads (RPKM) ...
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bioRxiv - Molecular Biology 2020Quote: ... The statistical test applied was the DGE (Differential Gene Expression) using the RNA-seq package with CLC Genomics Workbench© v 20.0 (Qiagen). The expression values for the transcripts were registered in TPM (Transcripts per Million ...
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bioRxiv - Microbiology 2020Quote: ... Gene expression was assessed using SYBR GreenER based-quantitative polymerase chain reaction (qPCR) or RT2 profiler arrays (Qiagen Ltd, UK). For SYBR GreenER based-qPCR analyses ...
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bioRxiv - Pathology 2022Quote: ... The N gene-specific primers were used to amplify 97 bp of SARS-CoV-2 N gene by conventional PCR and purified by Qiagen gel extraction kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: The plasmid #TH8-77 was digested by Not I and DNA fragment containing cdc8 gene fused with mNeongreen and a 40 aa linker were purified by gel extraction (Qiagen). Obtained DNA fragment was used for the transformation of MBY101 strain (h- ...
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bioRxiv - Cell Biology 2022Quote: The heatmaps relative to the ERK-dependent AP-1 target genes were generated by interrogating the Transcription Factor Target (TFT) collection (MSigDB) and TF binding site database by QIAGEN with the top DEGs between control and PD-treated Acomys ...
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bioRxiv - Molecular Biology 2020Quote: ... 5x Hot FIREPol EvaGreen qPCR mix plus for quantitative PCR (Solis Biodyne) was used for the reactions with a Corbett Rotor-Gene 6000 (Qiagen). The reactions were pipetted with a liquid handling system (Corbett CAS-1200 ...
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bioRxiv - Microbiology 2020Quote: ... construct pcDNA6B-nCoV-NSP12-FLAG encoding the RDRP gene (gift from Dr. George Stark, Cleveland Clinic) was digested with Xho1 and purified by Qiagen QIAquick PCR Purification Kit to be used as a standard in the RT-qPCR reaction ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was then diluted 25 times to serve as template for real-time quantitative PCR (RT-qPCR) using Rotor-Gene 6000 (Corbett Research, QIAgen), as previously described (Forlenza et al. ...
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bioRxiv - Microbiology 2021Quote: ... and a high resolution melt curve (HRM) in the end] using the primers listed in Table S2 on a Rotor-Gene Q instrument (Qiagen). rnpB was taken as reference for all genes ...
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bioRxiv - Microbiology 2021Quote: ... The relative abundance of bacterial 16S rRNA and methanotrophic pmoA genes in genomic DNA distributed across the CsCl gradients was determined by qPCR in a Corbett Rotor-Gene 6000 thermocycler (Qiagen) using primer sets P1(341f)/P2(534r ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... reverse transcription and amplification of the S gene were done with the one-step QuantiFast Sybr Green RT-PCR mix (Qiagen) as follows ...
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bioRxiv - Plant Biology 2021Quote: ... were set up with a CAS-1200N robotic liquid handling system (Corbett Research) and run for 50 cycles on a Rotor-Gene Q (Qiagen). Two technical replicates and three biological replicates were performed for each sample ...
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bioRxiv - Neuroscience 2021Quote: ... PCR triplicates of each cDNA (5 µL) were analyzed in a qPCR on a Rotor-Gene Q (Qiagen, Hilden, Germany) in a total reaction volume of 20 µL ...
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bioRxiv - Microbiology 2022Quote: ... Initial gene expression profiling was performed using the 96-well Human Cytokines & Chemokines RT2 Profiler PCR Array (PAHS-150ZC, Qiagen) according to manufacturer instructions ...
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bioRxiv - Genetics 2022Quote: ... It uses the same volumes as the standard Visium Spatial Gene Expression protocol but with an addition of 15µl Vapor-Lock (Qiagen, 981611) to each sample during the first incubation (fragmentation ...
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bioRxiv - Cancer Biology 2022Quote: The list of hypermethylated genes was imported into QIAGEN’s Ingenuity® Pathway Analysis (IPA®, QIAGEN Redwood City, www.qiagen.com/ingenuity). In IPA hypermethylated genes were mapped to molecular and cellular functions and to networks available in the ingenuity database and then ranked by score or p-value (p<0.05).
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bioRxiv - Cancer Biology 2022Quote: ... Pathway analysis of genes adjacent to identified tissue and cell-type specific methylation blocks was performed using Ingenuity Pathway Analysis (IPA)(42) (Qiagen) and Genomic Regions Enrichment of Annotations Tool (GREAT)(43) ...
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bioRxiv - Developmental Biology 2019Quote: ... Gene list enrichment analysis was performed using EnrichR 28 and Ingenuity Pathway Analysis IPA (www.qiagen.com/ingenuity, QIAGEN, Redwood City, CA) for genes with fold-change ± 1.5 ...
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bioRxiv - Genomics 2019Quote: ... A quantitative PCR reaction was carried out for checking the specificity of the samples using the Rotor-Gene Q cycler (Qiagen) and the ABsolute qPCR SYBR Green Mix from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time PCR (QPCR) using gene-specific primers (supplementary table S9) was performed using QuantiNova SYBR Green mix (Qiagen) on an MX3000P real-time PCR machine (Stratagene) ...