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Citations for Qiagen :
1251 - 1300 of 10000+ citations for Mouse Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from FACS purified Zeb1+ (Lineage−Sca-1+CD49fhi tdTomato+) or Zeb1− basal cells (Lineage−Sca-1+CD49fhitdTomato−) using a RNeasy micro kit (Qiagen). cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara) ...
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bioRxiv - Biochemistry 2020Quote: ... The restriction digestion efficiency was validated using 1% 1× TBE agarose gel electrophoresis and purified with a QIAquick PCR Purification Kit (Qiagen).
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bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from 1×106 MCF7 and 1×106 BT-474 cells (‘bulk’) using the RNeasy Mini Kit (74104, Qiagen) in combination with the QIAshredder (79654 ...
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bioRxiv - Microbiology 2023Quote: ... A volume of 1:1 of 70% ethanol was mixed with the upper phase before transfer on column (RNeasy Mini kit, Qiagen), and RNA was extracted according to manufacturer directions ...
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bioRxiv - Plant Biology 2023Quote: ... of g1090::XVE>>AXR3-1-mCherry and HS::AXR3-1 were extracted following the protocol (Plant Total RNA Mini Kit, QIAGEN). g1090::XVE>>AXR3-1-mCherry were transferred for 2 h on estradiol-containing medium and then harvested ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
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bioRxiv - Cancer Biology 2023Quote: ... Digested PCR fragments were ligated into the restricted vector at a 1:1 molar ratio using T4 DNA ligase and were purified using the MiniElute PCR purification kit (Qiagen). Purified ligated plasmids were electroporated into MegaX DH10B (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... fungal DNA was extracted independently from 1 mL of crude conidial suspensions (between 108 and 109 spores mL-1) using the DNeasy 96 Plant Kit (Qiagen) following the manufacturer’s instructions (Gautier et al. ...
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bioRxiv - Physiology 2024Quote: ... After chloroform incubation and centrifugation per manufacture instructions RNA-containing phase was diluted with 70% ethanol 1:1 and RNA isolation was performed using RNeasy Mini Kit (Qiagen). For tissues ...
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bioRxiv - Physiology 2022Quote: ... Mouse Obesity (PAMM- 017ZC-12) array and Mouse Aging (PAMM-178ZC) from Qiagen (Maryland, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from the indicated mouse tissues and cultured cells using RNeasy® Lipid Tissue Mini Kit (Qiagen, Valencia, CA, USA). Reverse transcription reactions containing 1 μg of total RNA were performed using PrimeScript™ (Takara ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from donor cecal contents and day 0 conventionalized mouse feces using the Qiagen PowerFecal DNA Kit (Qiagen, Hilden, Germany; 12830) following the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... eyes were enucleated and retinas were immediately dissected and total RNA was extracted from mouse retinal tissue using Qiagen RNeasy Mini Kit as per manufacturers protocol (Qiagen, Valencia, CA, USA) and reverse-transcribed using iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: RNA was extracted from mouse islets (∼300 islets per sample, pooled from ∼10 mice of the same genotype) using RNAeasy Micro Kit (Qiagen, Valencia CA, USA) as per manufactures instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from the mouse cells/tissues and human cell lines using the RNeasy® Mini Kit (Qiagen Inc., Germantown, MD). For human platelets and MEG-01 cells ...
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bioRxiv - Molecular Biology 2021Quote: ... Biological pathway enrichment was performed on the synergy gene signature using Ingenuity Pathway Analysis version 01-07 (QIAGEN). Fisher’s exact t-test was used to assess significantly associated canonical signaling pathways ...
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bioRxiv - Genomics 2019Quote: Differential gene expression data were analyzed using the IPA software suite (https://www.qiagenbioinformatics.com/products/ingenuitypathway-analysis) (QIAGEN Inc). Genes related to liver fibrosis and hepatocellular carcinoma were obtained by searching for the terms “liver fibrosis” and “Hepatocellular carcinoma” in the Diseases and Functions search field ...
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bioRxiv - Immunology 2019Quote: ... CSF and whole blood for transcriptional profiling were collected in blood PAX-gene® (Pre-AnalytiX, Qiagen, USA) tubes ...
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bioRxiv - Immunology 2021Quote: ... machine with probes for ACTB (Hs01060665_g1) as housekeeping gene and with either XIST (Hs01079824_m1) or EIF3AY (Hs01040047) all from Qiagen. Values undeterminable were given cycle values of 40 for quantification purposes.
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bioRxiv - Biochemistry 2020Quote: ... Three technical replicates of each sample (ie biological replicate) were analysed using a Rotor-Gene Q 2plex (Qiagen), plus control reactions without cDNA template for each primer pair ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time qRT-PCRs were run in a Rotor-Gene Q real-time PCR cycler (Qiagen, Hilden, Germany) using the following program ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cDNA samples were amplified in technical duplicates in presence of gene specific primers (QuantiTect® Primer Assays, Qiagen) and QuantiTect® SYBR Green Master Mix (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... Differentially expressed genes were identified using the bioconductor package limma (3.42.2) with FDR<0.1 and the resulting gene lists were used as input for Ingenuity pathway analysis (Qiagen). Weighted gene co-expression network analysis was conducted using WGCNA R-package (ver 1.69) ...
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bioRxiv - Neuroscience 2022Quote: ... Measurements were completed in triplicate and results were analyzed with a Rotor-Gene-Q software version 2.3 (Qiagen). Relative expression levels of each target gene were calculated using the comparative Ct method and HPRT1 as a housekeeping gene for normalization ...
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bioRxiv - Microbiology 2021Quote: ... Functional and network analysis of statistically significant gene expression changes was performed using Ingenuity Pathways Analysis (IPA, QIAGEN). Significant canonical pathways are identified from IPA based on enrichment of the zscore.
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bioRxiv - Genetics 2019Quote: The sets of ADHD-BMI and ADHD-obesity overlapping genes were then individually tested for enrichment of canonical pathways using Ingenuity Pathway Analysis (http://www.ingenuity.com; QIAGEN Bioinformatics ...
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bioRxiv - Microbiology 2019Quote: ... Detection of Wolbachia was carried out by real-time PCR on a Rotor-Gene Q machine (Qiagen, Australia) using strain-specific primers and probes ...
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bioRxiv - Neuroscience 2019Quote: ... The cDNA was measured in triplicates by RT-qPCR (reaction volume 15 l) using Rotor-Gene Q (Qiagen) and Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent) ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were treated with a pool of four small siRNAs per gene with the following sequences (Qiagen 1027280):
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bioRxiv - Physiology 2019Quote: The mRNA expression of 34 metabolic genes was analyzed using a custom RT2 Profiler PCR Array (Qiagen, USA). First ...
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bioRxiv - Immunology 2019Quote: ... Promoter-T3SS pairs were cloned upstream of the CatB gene and inserted separately into pQE30 (Qiagen, Hilden, Germany). Parallel constructs were made with CatB gene replaced by eGFP to produce expression plasmids used for imaging studies ...
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bioRxiv - Cell Biology 2019Quote: The absolute quantification by quantitative real-time PCR (qPCR) was performed with the Rotor-Gene Q thermocycler (Qiagen) and the QuantiNova SYBR Green PCR Kit ...
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bioRxiv - Immunology 2021Quote: ... using cycle threshold (Ct) values acquired from the amplification curve using the Rotor-Gene Q instrumentation software (Qiagen).
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bioRxiv - Immunology 2020Quote: ... Relative gene expression was calculated using the Qiagen webportal PCR array data analysis web-based software (Qiagen, Germany). Differential expression (up and down regulation ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a customized RT2 profiler array for selected 89 genes according to the manufacturer’s instructions (Qiagen) and run on QuantStudio 7 Flex (software v1.0) ...
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bioRxiv - Cancer Biology 2022Quote: ... Knockdown of EN2 gene was conducted by introducing four different siRNA primers into the cells (Qiagen, Hilden, Germany) (Table 2) ...
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bioRxiv - Cell Biology 2022Quote: ... Genes with significantly altered expression between PTPN23H/H and PTPN23+/+ IAT were analyzed with Ingenuity Pathway Analysis (Qiagen) to identify impacted pathways and candidate upstream drivers ...
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bioRxiv - Microbiology 2022Quote: ... one-step RT-qPCR was performed using SensiFAST™ SYBR® Lo-ROX One-Step Kit (Meridian Bioscience) with 2μL of RNA samples according to the manufacturer’s protocol on a Rotor-Gene Q apparatus (QIAGEN) using primers listed in Table 2 ...
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bioRxiv - Microbiology 2023Quote: ... The amplification reaction was conducted using a Rotor-Gene-Q PCR Cycler Real-Time PCR system (Qiagen, Australia). The cycling parameters were as follows ...
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bioRxiv - Microbiology 2023Quote: Full-length sdeC genes were amplified by PCR and cloned into the bacterial expression vector pQE-80L (Qiagen) with BamHI and SacI restriction sites to generate a 6xHis-SdeC-StrepII fusion construct (Supplemental Table 1) ...
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bioRxiv - Molecular Biology 2023Quote: ... ARPE-19 cells were transfected with a HIF-luciferase reporter gene construct (Cignal Lenti HIF Reporter, Qiagen, Netherlands). The HIF-luciferase construct encodes a firefly luciferase gene under the control of the hypoxia response element ...
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bioRxiv - Microbiology 2023Quote: ... The presence and nucleotide sequence of resistance genes were manually verified using the QIAGEN CLC genomics workbench 21.0.3 (QIAGEN Digital Insights ...
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bioRxiv - Plant Biology 2024Quote: ... The library was checked with Qubit and real-time PCR (Rotor-Gene Q thermal cycler, Qiagen, Hilden, Germany) for quantification and bioanalyzer for size distribution detection ...
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bioRxiv - Neuroscience 2024Quote: ... Differentially expressed genes were identified using the bioconductor package limma (3.42.2) with FDR < 0.1 and the resulting gene lists were used as input for Ingenuity pathway analysis (Qiagen). Weighted gene co-expression network analysis was conducted using WGCNA R-package (ver 1.69) ...
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bioRxiv - Cell Biology 2024Quote: ... Gene expression changes associated with osteogenesis were assessed using an RT2 Profiler™ PCR Array (PAMM-026Z, Qiagen). A fold regulation threshold of 2 was used to determine up- or down-regulation of each gene in male and female PAE mice compared to control and data is presented as log2 fold change ...
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bioRxiv - Physiology 2020Quote: ... protein was extracted (Qiagen Tissue Lyser) from ∼50 mg frozen liver in tissue lysis buffer 80 ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL Protein Precipitation Solution (QIAGEN) was added ...
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bioRxiv - Cancer Biology 2023Quote: Advanced Protein Purification buffer (APP; Qiagen): the APP buffer contains zinc chloride to precipitate protein at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and The Protein Complex Suite (Qiagen). One microliter of 4.6 mg/ml protein sample suspended in crystallization buffer (25 mM Tris-HCl ...