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Citations for Qiagen :
1551 - 1600 of 10000+ citations for Mouse Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). Transcript quantification was performed in 96-well plates ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). Transcript quantification was performed in 96-well plates ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour at room temperature followed by isolation with a minElute RNA Cleanup Kit (Qiagen). RNA concentrations were measured by Nanodrop and total RNA integrity assayed using an Agilent Bioanalyzer ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total mRNA from each sample was retrotranscribed using QuantiTech reverse Transcription Kit (Qiagen). Real-Time PCR was performed using SYBR Green Master Mix (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from 1 ml of overnight culture (DNeasy Blood and Tissue Kit—Qiagen) pre-treated with 100 μg of lysostaphin (Sigma cat ...
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bioRxiv - Genomics 2023Quote: ... cfDNA was isolated from 1 mL of plasma using the QIAGEN Circulating Nucleic Acids Kit (QIAGEN), eluted in AE buffer ...
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bioRxiv - Genomics 2024Quote: ... 1 mL of PE buffer and 100 µL of EB buffer (MinElute PCR Purification Kit, QIAGEN). The silica columns were placed in a collection tube to dry and later in a 1.5 mL DNA lo-bind tube (Eppendorf ...
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bioRxiv - Neuroscience 2022Quote: PON2 level was quantified in the mouse whole brain homogenate in 100 mg per 1 ml of QIAzol® lysis reagent (Qiagen, Hilden, Germany). mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc. ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified using Ni-NTA agarose (Qiagen) and eluted using 300 mM imidazole in purification buffer ...
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bioRxiv - Genetics 2020Quote: ... Proteins were purified with Ni-NTA beads (Qiagen). Proteins and beads were washed 3 times with protein purification lysis buffer before incubating the beads with elution buffer (400 mM imidazole in protein purification lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was purified using Ni-NTA agarose (Qiagen) eluting with 300 mM imidazole ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were purified with Ni-NTA Resin (Qiagen) using standard protocols ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were purified using Ni-NTA agarose (Qiagen) resin first ...
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bioRxiv - Biochemistry 2022Quote: ... Protein Center (Thermo) and Ingenuity Pathways Analysis (Qiagen). For protein center analysis ...
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bioRxiv - Synthetic Biology 2022Quote: ... Proteins were purified on Ni-NTA resin (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Proteins were purified using Ni-NTA agarose (QIAGEN), followed by size-exclusion chromatography using HiLoad 16/600 Superdex200 columns (GE Healthcare Life Sciences) ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were purified using Ni-NTA (Qiagen), and the His tag was cleaved by the Tev protease ...
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bioRxiv - Microbiology 2023Quote: ... SrtC2 protein was purified using Ni-NTA (Qiagen) affinity chromatography with the addition of 5 mM β-mercaptoethanol in all buffers ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with NiNTA agarose (Qiagen). The eluate was further purified over a Source 15 Q column (Cytiva) ...
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bioRxiv - Developmental Biology 2020Quote: Differential gene expression results derived from the scRNA-seq quantifications were analyzed using Ingenuity Pathway Analysis (IPA, QIAGEN Inc., Hilden, Germany). Each cluster was individually analyzed to examine the relationship between its highly significantly up- or downregulated genes between CO versus 4OHT ...
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bioRxiv - Molecular Biology 2020Quote: ... level relative to nuclear gene (β actin) by real-time PCR in total DNA isolated from INS1 cells by QIAamp DNA minikit (Qiagen). Rat primers were mt-ND6 ...
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bioRxiv - Genomics 2019Quote: All pathway and biological process enrichment analyses and SE-associated gene categorizations were conducted using Ingenuity® Pathway Analysis Software (QIAGEN).
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bioRxiv - Cell Biology 2019Quote: ... Quantitative PCR were performed in triplicates using PerfeCTa SYBR® Green SuperMix (Quantabio, Beverly, MA, USA) according to the manufacturer’s guidelines and assayed with a Rotor Gene 6000 (Qiagen, Venlo, Netherlands). Primers are listed in Supplementary table S1.
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bioRxiv - Immunology 2019Quote: ... the inserted coding regions of each gene were transferred to the multi-cloning site of expression vector pQE30 (Qiagen, Hilden, Germany) for the expression of a hexahistidine-tagged protein in E ...
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bioRxiv - Neuroscience 2020Quote: ... The following primers were used to assess the relative level of GAT-3 mRNA: Slc6a11 Rn.10545 in comparison to that of cyclophilin A (Peptidylprolyl isomerase A) used as housekeeping gene (Qiagen, UK). Real-time-PCR was performed on the CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... The list of MMP and TIMP genes from the microarray data was loaded into QIAGEN’s Ingenuity Pathway Analysis software (IPA®, QIAGEN, USA) to perform biological network and functional analyses.
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bioRxiv - Biophysics 2021Quote: Thermal stability of CagFbFP and all mutated variants were measured using the Rotor-Gene Q real-time PCR cycler (Qiagen, Germany) with fluorescence excitation at 470 nm and fluorescence detection at 510 nm ...
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bioRxiv - Microbiology 2020Quote: ... the variable region genes from each single cell were amplified in a reverse transcriptase polymerase chain reaction (RT-PCR: QIAGEN, Germany) using a cocktail of sense primers specific for the leader region and antisense primers to the Cγ constant region for heavy chain and Cκ and Cγ for light chain ...
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bioRxiv - Immunology 2022Quote: ... Downstream pathway analysis was performed using the gene set enrichment analysis (GSEA) tools provided by the Broad Institute 32 or ingenuity pathway analysis (IPA) (QIAGEN Inc.) 35.
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bioRxiv - Cell Biology 2019Quote: ... 25-50ng cDNA and 10 pmol gene specific primer were used in a 10 μl SYBR green reaction mix (Qiagen, 204056) for Quantitative RT-PCR (Supplemental Table 1 ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines were generated by transfecting the DNA constructs containing the gene of interest with Effectene transfection reagent (Qiagen, Hilden, Germany), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Ct values of the technical replicates were averaged and used for relative gene expression analysis in the REST 2009 software (Qiagen, Germany) [46] ...
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bioRxiv - Genetics 2020Quote: ... Host genes data were assigned to the core analysis of Ingenuity® Pathway Analysis (IPA®, QIAGEN Redwood City,www.qiagen.com/ingenuity). Pathway enrichment of the gene set was performed by ReactomePA and ClusterProfiler R/Bioconductor packages 49.
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bioRxiv - Cell Biology 2021Quote: Plasmids containing a tagged gene of interest were transfected into Drosophila S2 cells using Effectene transfection reagent following the manufacturer supplied protocol (Qiagen 301425). S2 cells were passaged 24h before transfection ...
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bioRxiv - Molecular Biology 2021Quote: Hepatic gene expression was investigated using qPCR arrays designed for human (RT2 Profiler PCR arrays – ‘Human Fatty Liver’, Qiagen France SAS). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... SYBR I was added to the samples after the reaction was completed and the fluorescence measured in a Real-time PCR machine (Rotor-Gene Q, Qiagen, USA). Triplicates for each conditions were measured and the mean value calculated ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was monitored on Rotor-Gene® Q-Pure Detection System (Software Ver. 2, Qiagen Inc., Valencia, CA, USA) and performed using QuantiFast SYBR® Green PCR Master Mix (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: ... Reactions were run three times in quadruplicates in the Rotor-Gene Q Real-Time PCR Detection System (QIAGEN, Germantown, Maryland, USA) with the following reaction conditions ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... and 25 pmol primers in a 25 μL reaction volume and subjected to qPCR using Rotor-Gene Q (Qiagen, Venlo, Netherlands). We amplified the tdTomato gene sequence for vector genome quantification using the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... β-actin as housekeeping gene (Forward: 5’-CAGCCATGTACGTTGCTATCCAGG-3; reverse: 5’-AGGTCCAGACGCAGGATGGCA-3’) and 2X RT2 SYBR Green qPCR master mix (Qiagen, UK). The relative fold change in gene expression was analysed using the double delta Cq analysis (2-ΔΔCq ...
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bioRxiv - Developmental Biology 2024Quote: Gene expression was determined using the RT2 Profiler PCR Array (SYBR ® Green PCR Mastermix, CAPM11273; SABioscences-QIAGEN, Valencia, CA, USA) and SYBR Green qPCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... a monoclonal mouse Strep-tag antibodies (#34850, Qiagen), a monoclonal anti-actin antibody (A5441 ...
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bioRxiv - Cell Biology 2023Quote: ... or siRNA for Anxa2 (Mouse, Qiagen, Mm_Anxa2_3, SI00167496).
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bioRxiv - Biochemistry 2023Quote: Mouse EL4 genomic DNA was isolated by QIAGEN Blood & Cell Culture DNA Kit (QIAGEN ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse tissues were homogenized using TissueLyser II (Qiagen) and stainless-steel beads (5 mm ...
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA (gDNA) was extracted from 1-5×106 cells using DNeasy Blood and Tissue Kit (Qiagen) and 250 ng gDNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted from 1 ml of overnight culture using a QIAamp DNA Mini Kit (Qiagen). Illumina MiSeq sequencing operated by the Functional Genomics Centre Zurich and Novogene (Cambridge ...
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bioRxiv - Immunology 2021Quote: DNA was isolated from 1 × 106 PBMC using the DNeasy Blood & Tissue Kit (Qiagen, Cat No 69506), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from CTi400 and N/TERT-1 cells using RNeasy Plus mini kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Total RNA from PGC fibroblast and DF-1 cells were extracted using an RNeasy mini kit (Qiagen), following manufacturer’s instructions ...