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Citations for Qiagen :
1201 - 1250 of 4884 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... PCR assays were performed based on previous published method (Gonatopoulos-Pournatzis et al., 2018) OneStep RT-PCR kit (QIAGEN) was used according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... and subjected to real-time RT-PCR for viral RNA quantitation [40] using QuantiTect Probe RT-PCR Kit (Qiagen) and QuantStudio 5 (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: ... cDNA of each gene target was reverse-transcribed and PCR amplified with the PyroMark OneStep RT-PCR kit (QIAGEN) using one biotinylated (reverse ...
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bioRxiv - Microbiology 2022Quote: ... Real-time PCR was performed using RT2 Profiler PCR array in combination with RT2 SYBR green master mix (Qiagen) using a QuantStudio 6 Flex real-time PCR system ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR was performed using a Q-Rex thermal cycler using the Rotor-Gene SYBR green PCR kit (Qiagen). Primer sequences were ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of cDNA was then added to a PCR mix containing 12.5 μl 2× Multiplex PCR mix (Qiagen), 9 μl H2O ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were confirmed by agarose gel electrophoresis using 0.8-1% agarose and purified using PCR purification kit (Qiagen). In case of NS1 gene ...
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bioRxiv - Genetics 2022Quote: ... PCR product was pooled and purified two times with a QIAquick PCR Purification Kit (Qiagen, Inc., Valencia, CA, USA) and DNA quantity was measured on an Agilent 2200 TapeStation (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... was PCR amplified from plasmid (pSH515) containing the native His4 gene promoter and purified by PCR cleanup kit (Qiagen). DNA was eluted from the spin column with 10 mM Tris-HCl ...
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bioRxiv - Genetics 2020Quote: ... and the target sites were PCR amplified by nested PCR amplification and purified by a Gel Extraction Kit (QIAGEN) for deep sequencing.
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bioRxiv - Genetics 2020Quote: ... and the target sites were PCR-amplified by nested PCR amplification and purified by a Gel Extraction Kit (QIAGEN) for deep sequencing.
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bioRxiv - Genetics 2020Quote: ... Multiplex polymerase chain reaction (PCR) was performed using three primer pairs and the Multiplex PCR Kit (QIAGEN, Hilden, Germany). Each reaction contained 2× QIAGEN multiplex PCR master mix ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR product was separated using 1.5% agarose gels and purified using the QIAquick PCR & Gel Cleanup Kit (Qiagen). Purified PCR products (300 ng ...
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bioRxiv - Microbiology 2023Quote: ... and PCR products were purified and concentrated to 500 ng/uL using a MinElute PCR product purification kit (Qiagen). Strains containing pKD46 were grown at 30 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription and quantitative real-time PCR were performed with the QuantiFast SYBR Green PCR Kit (Qiagen, Hilden, Germany). Since the endogenous retrovirus envelope comprises only one exon ...
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bioRxiv - Genomics 2022Quote: ... The two PCR reactions for each sample were pooled and cleaned up using the QIAquick PCR Purification Kit (Qiagen) and samples eluted in 15μL Elution Buffer (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: Quantitative PCR (qPCR) for miR-519a-3p was performed in triplicate using miRCURY LNA SYBR Green PCR kit (Qiagen) and hsa-miR-519a-3p ...
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bioRxiv - Molecular Biology 2023Quote: ... The excess dsDNA was removed by 3 rounds of PCR clean up with QIAquick PCR Purification Kit (Qiagen, 28106).
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bioRxiv - Plant Biology 2023Quote: ... The prepared libraries were enriched using 15 cycles of PCR and purified by the QIAquick PCR purification kit (Qiagen). The Agilent 2100 Bioanalyzer was used to control the size and purity of the samples using the Agilent High Sensitivity DNA Kit ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed with 200 ng of extracted total RNA using a Qiagen OneStep RT-PCR Kit (Qiagen). The primers used were a forward primer (Hokkaido System Science ...
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bioRxiv - Microbiology 2023Quote: ... SYBR green real-time PCR assay was carried out in a 20μL PCR mixture volume consisting of 10 μL of 2X Quantitect SYBR green RT-PCR Master Mix (Qiagen) containing HotStarTaq DNA polymerase ...
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bioRxiv - Immunology 2023Quote: ... Illumina adapters were completed by a second PCR carried out with the Qiagen Multiplex PCR kit (Qiagen, Valencia, CA), using 1 μl of the first PCR product as the template in a 20 μl reaction with the following program ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments that were generated by the polymerase chain reaction (PCR) were purified using the PCR Purification Kit (Qiagen), Monarch® PCR & DNA Cleanup Kit (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR reactions for each sample were pooled and cleaned up with the QIAquick PCR purification kit (Qiagen 28104). Using the pooled PCR1 products ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were assessed on a gel and purified using the Qiaquick PCR Cleanup kit (Qiagen, cat. no. 28104). Plasmids for Foxd-3 and Sox10 mRNA probe synthesis were from C ...
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bioRxiv - Microbiology 2024Quote: ... and the PCR products of each biological replicate were pooled and purified using the QIAquick PCR Purification Kit (Qiagen). We followed the detailed protocol for library preparation as given in Agarwal et al ...
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bioRxiv - Genetics 2024Quote: ... The four PCR replicates obtained from each sample were pooled and concentrated with the MinElute PCR Purification Kit (Qiagen) before gel electrophoresis.
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bioRxiv - Microbiology 2024Quote: ... were quantitated using one-step qRT-PCR with 2µl of RNA input was conducted using the QuantiNova Probe RT-PCR kit (Qiagen) according to the manufacturer’s instructions and run on a QuantStudio 6 instrument (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... Deletion efficiency was confirmed with Sanger sequencing on the column-purified PCR fragments (QIAquick PCR Purification Kit, Qiagen, 28104), using the forward PCR primer for sequencing ...
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bioRxiv - Microbiology 2020Quote: ... Translocation positive bands were re-amplified from the corresponding first round PCR in second round PCR reactions and purified with QIAquick Gel Extraction Kit (Qiagen) for DNA sequencing.
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bioRxiv - Plant Biology 2020Quote: Quantitative real-time PCR was performed in a volume of 5 mL QuantiTect Probe PCR Kit (Qiagen GmbH, Hilden, Germany) kit and an ABI 7900HT fast real-time PCR system (ThermoFisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out using a QuantiFast SYBR Green PCR kit (QIAGEN, 204056) on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA (200 ng) was used for quantitative PCR with the Human Cellular Senescence RT2 Profiler™ PCR Array (Qiagen) containing 84 key genes involved in the initiation and progression of the biological process causing cells to lose the ability to divide ...
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bioRxiv - Microbiology 2021Quote: ... 250 ng of cDNA were used as the template for real-time PCR using QUANTITECT SYBR GREEN PCR KIT (QIAgen). Reactions were performed using the ABI 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2020Quote: ... Two gRNAs targeting the first and second coding exons were generated via PCR reaction and purified using QIAquick PCR Purification Kit (QIAGEN). The sequence targets were ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR products were detected by gel electrophoresis and purified with the MinElute PCR Purification Kit (Qiagen, Valencia, CA, USA) and sequenced.
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bioRxiv - Developmental Biology 2020Quote: ... A second PCR was performed using the same parameters and the products were purified with a QIAquick PCR Purification Kit (Qiagen). Single stranded riboprobes were transcribed with a Roche DIG Labeling Kit (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... Each sample was then amplified by the determined number of PCR cycles and then purified (MinElute PCR Purification Kit, Qiagen). Final libraries were then size selected for fragments between 100-1000bp using AMPure XP beds (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... Positive sense vRNA and GAPDH transcripts were measured by qRT-PCR using the QuantiTect SYBR Green RT-PCR kit (Qiagen) on an Applied Biosystems 7300 machine ...
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bioRxiv - Immunology 2021Quote: ... PRRSV-2 RNA was used to amplify cDNA encoding the full-length of N gene by RT-PCR (One-step RT-PCR, QIAGEN) using specific primers with the forward containing a T7 promoter sequence at the 5’ end ...
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bioRxiv - Molecular Biology 2021Quote: ... Oligonucleotides containing shRNA inserts were PCR-amplified with primers 5’-TCTCGAATTCTAGCCCCTTGAAGTCCGAGGCAGTAGGC-3’ and 5’-TGAACTCGAGAAGGTATATTGCTGTTGACAGTGAGCG-3’ and purified with QIAquick PCR Purification Kit (Qiagen). shRNA inserts and miRE18_LT3GEPIR_Ren714 backbone (inducible via Tet-On system ...
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bioRxiv - Physiology 2020Quote: ... ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen) on an iCycler instrument (BioRad) ...
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bioRxiv - Genetics 2020Quote: ... Because of clear evidence of the response of the pGL4.74 Renilla normalisation control plasmid to PMA treatments and co-transfection with the EGR1 expression vector Lucia activity levels were normalised against quantitative PCR (qPCR) of levels of Lucia gene DNA present in cell extracts recovered using Qiagen PCR cleanup kits (Qiagen). Lucia luciferase activity was normalised against the crossing point (Cp ...
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bioRxiv - Synthetic Biology 2020Quote: All nicked PCR products (obtained either via PfAgo or Cas9n reactions) were purified using the Qiaquick PCR purification kit (QIAGEN) and eluted in ddH2O ...
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bioRxiv - Immunology 2021Quote: ... The PCR products were subjected to 1 % agarose gel electrophoresis for analysis and purified with QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Each AES was then amplified by PCR on a 3 kb fragment using primer X5 and X6 and purified using QIAquick PCR Purification kit (Qiagen). For gene yrbE4A ...
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bioRxiv - Microbiology 2021Quote: ... A total of 25 cycles of PCR was performed and the expected PCR products (500-600 bp) were gel purified (Qiagen). NGS was performed on the Ion S5 GeneStudio system ...
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bioRxiv - Microbiology 2021Quote: ... The remaining 18 μl of RT-PCR DNA was purified by a QIAquick PCR Purification kit (Qiagen, Germantown, MD, USA). Sequences of the purified RT-PCR products were generated using a BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... A total of 25 cycles of PCR was performed and the expected PCR products (500-600 bp) were gel purified (Qiagen). NGS was performed on the Ion S5 GeneStudio system ...
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bioRxiv - Microbiology 2020Quote: ... Real-time quantitative PCR was performed using Mx3000pTM SYBR Green real-time quantitative PCR Analyzer (QIAGEN Biotechnology Co, Ltd, China). Briefly ...