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Citations for Qiagen :
1001 - 1050 of 4884 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... rpsL and qoxA transcripts by qRT‒PCR was performed with QuantiFast SYBR Green RT‒PCR Kit (Qiagen) using the Quantstudio3 system (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: PCR amplification of the RNA was completed using the One-step Syber Green RT-PCR kit (Qiagen). 25ng of total RNA was added to a master mix reaction of the provided RT mix ...
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bioRxiv - Genomics 2023Quote: The PCR was performed with the Multiplex PCR Kit (Type-It Microsatellite master mix; Qiagen, Hilden, Germany). The SSR primer mix consisted of eight primers (see Table 2 and Table 3) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR trials were run on the DNA extracts using the Qiagen Multiplex PCR kit (Qiagen, Hilden, Germany), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products were purified and concentrated with the QIAquick PCR Purification kit (Qiagen, cat. no. 28106). Homology arm and ultramer sequences are listed in Table S8.
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bioRxiv - Biophysics 2022Quote: ... The PCRs of each sample were then pooled and purified using the MinElute PCR Purification Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... PCRs for DH31 and DH31R alleles were conducted using the Taq PCR Master Mix Kit (Qiagen, # 201445) and all primers were synthesized by Microsynth (Balgach ...
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bioRxiv - Cell Biology 2023Quote: ... BAG2 and ATG101 were amplified by RT-PCR from HEK293T cDNA (Qiagen QuantiTect RT-PCR kit, 205311) using primers oNDS227 and oNDS228 for ATG101 ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products were then pooled in each group and purified using QIAquick PCR Purification Kit (Qiagen). Final NGS libraries were generated using 2ng of the purified 1st PCR product using the dual-indexing Illumina-compatible DNA HT Dual Index kit (Takara #R4000660,R400661) ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was amplified by polymerase chain reaction (PCR) with QuantiTect Sybr Green PCR Kit (Qiagen, Germantown, MD) on an Applied Biosystems QuantStudio 7 Flex real-time quantitative PCR instrument ...
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bioRxiv - Biophysics 2024Quote: ... dsDNA templates were generated from template-less PCR and purified with MinElute PCR Purification Kit (Qiagen 28004). To convert dsDNA to ssRNA crRNA ...
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bioRxiv - Immunology 2024Quote: ... Illumina barcodes and indices were added with a 2nd round of PCR using Multiplex PCR Kits (Qiagen), as previously described (Rosenfeld et al. ...
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bioRxiv - Cell Biology 2024Quote: ... DNA fragments were PCR-amplified and subsequently purified using the MinElute PCR Purification Kit (Qiagen, Maryland, USA). The ATAC-seq library was prepared as described protocol113 ...
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bioRxiv - Biophysics 2020Quote: ... reaction mix (for PCR program details see Supplementary Table 1) and purified with QIAquick PCR Purification Kit (QIAGEN). The Fps1-mGFP strain was created by transforming the BY4741 background strain with 100 µL of the purified mGFP-HIS3 fragment with standard LiAc protocol to allow for homologous recombination 34 ...
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bioRxiv - Genetics 2021Quote: ... The PCR mix (final volume of 15.75 µL) consisted of: 7.5 µL Type-it Multiplex PCR Master Mix (QIAGEN), 3 µL Q solution (5X) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids and PCR products were purified with QIAprep plasmid Miniprep kit and QiaQuick PCR purification kit respectively (Qiagen). Plasmid Sanger sequencing was performed by Eurofins Genomics ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time PCR was performed using the Custom RT² Profiler PCR Array according to the manufacturer’s instructions (Qiagen), and detection was performed using the ABI Prism® 7500 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed using TaKaRa Ex Taq® DNA Polymerase and the PCR products were purified by Qiagen PCR purification kit ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription and subsequent PCR amplification (RT-PCR1) were performed using the Qiagen OneStep RT PCR mix (Qiagen). The cDNA was selected and unused primers were removed by SPRIselect bead selection (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time qRT-PCRs were run in a Rotor-Gene Q real-time PCR cycler (Qiagen, Hilden, Germany) using the following program ...
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bioRxiv - Cancer Biology 2020Quote: ... gp130 expression was assessed by quantitative PCR on re-amplified and purified (Qiagen PCR Purification Kit, Qiagen, Germany) WTA products of single cells as previously described 74 ...
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bioRxiv - Cancer Biology 2020Quote: ... gp130 expression was assessed by quantitative PCR on re-amplified and purified (Qiagen PCR Purification Kit, Qiagen, Germany) WTA products of single cells as previously described 74 ...
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bioRxiv - Cancer Biology 2020Quote: ... Expression of miRNA was evaluated by q-PCR using a miScript SYBR@Green PCR kit (Cat # 218073, Qiagen).Screening of miRNA expression for 84 different miRNAs was performed on Exo-RNA isolated of CM cell lines and serum exosomes ...
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bioRxiv - Microbiology 2020Quote: ... RNA samples were subjected to KSHV real-time PCR array using the QuantiTect Probe RT-PCR Kit (Qiagen). The copy number of each transcripts was normalized to that of GAPDH transcripts and the ratio of treated versus untreated cells was calculated.
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bioRxiv - Neuroscience 2021Quote: ... cDNA was processed for real time q-PCR using SYBR-green (QuantiFast SYBR Green RT-PCR Kit, Qiagen) and determination of ΔΔCT-values was done on the CFX Maestro Software (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was reverse transcribed and quantified by real-time PCR using the OneStep RT-PCR kit (Qiagen) as described previously(40 ...
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bioRxiv - Cell Biology 2020Quote: ... All PCR products were purified using either the QIAquick® PCR Purification Kit (Art. No. 28106, QIAGEN®) or the DNA Clean & Concentrator-5 Kit (Art ...
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Deconstructing the role of iron and heme in the structure of healthy human gut microbial communitiesbioRxiv - Microbiology 2022Quote: ... PCR products were individually cleaned up and quantified using the UltraClean 96 PCR Cleanup Kit (Qiagen 12596-4) and the Quant-iT dsDNA High Sensitivity Assay kit (Invitrogen Q33120 ...
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bioRxiv - Microbiology 2021Quote: ... Clones were screened for correctly assembled DNA fragments by multiplex PCR using the QIAGEN Multiplex PCR kit (QIAGEN) according to the manufacturers’ instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and PCR purified (Q5 High-Fidelity 2X MasterMix, New England Biosciences #M0492L; QiaQuick PCR Purification Kit, Qiagen #28104). Single colonies were grown overnight and purified using QIAprep Spin Miniprep Kit (Qiagen #27104) ...
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bioRxiv - Genetics 2021Quote: Long PCR products were purified by gel electrophoresis and short ones with the QIAquick PCR purification kit (Qiagen). Terminal adenine overhangs were added using Taq polymerase in the presence of dATP ...
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bioRxiv - Biophysics 2021Quote: ... The PCR amplified cDNA construct (from 140–160 bp) was purified using a QIAquick PCR Purification kit (Qiagen). The purified cDNA was directly sequenced using an Illumina MiSeq 2000 platform (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... All samples were pooled in equimolar ratio and PCR purified pooled sample with QIAquick PCR Purification Kit (Qiagen). The purified sample was then run on a 2% E-Gel and the correct size band was extracted for PCR2 (250-270 bp ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR was performed using QIAGEN’s RT2 Profiler™ PCR Array Human Cell Death PathwayFinder (QIAGEN, Hilden, Germany). RT-PCR data was analyzed and visualized for pathway analysis by GeneSpring GX 14.9 (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... The remaining 45 μL of PCR products were purified using a Qiaquick® PCR purification kit (Qiagen, Germany). The purified PCR amplicons were sequenced by Sanger sequencing at GeneWiz ...
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bioRxiv - Plant Biology 2022Quote: ... The adaptor ligated DNA was amplified by PCR and then purified on Qiagen PCR purification kit (QIAGEN, 28104) to produce the libraries for sequencing ...
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bioRxiv - Molecular Biology 2023Quote: dPCR was performed using the Qiagen QIAcuity Digital PCR system (Instrument: QIAcuity-00412; Software: QIAcuity Software Suite 2.1.7.182) and the QIAcuity EG PCR Kit (Qiagen; 250111) following manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: QRT-PCR was performed by using miScript SYBR® Green PCR Kit (Qiagen, Hilden,Germany Cat No: 218073) with the high-throughput Light Cycler 480 II Real-Time PCR system(Roche ...
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Development of immortalized rhesus macaque kidney cells supporting infection with a panel of virusesbioRxiv - Microbiology 2022Quote: RNA for quantitative real-time PCR (qRT-PCR) was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the protocol of the manufacturer and eluted in a final volume of 25 μl RNase-free water ...
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bioRxiv - Plant Biology 2022Quote: ... Real-time PCR was conducted with cDNA as the template using the Quantitect SYBR Green PCR kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... The purified PCR product was digested with HindIII and BamHI and purified by the PCR purification kit (Qiagen). The purified tapA promoter and gfp-lcn DNA fragments were mixed and ligated with pDG1664 digested with EcoRI and BamHI ...
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bioRxiv - Developmental Biology 2024Quote: ... Inserts were PCR amplified using M13 primers and template plasmid was digested with DpnI before PCR purification (Qiagen). Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega) ...
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bioRxiv - Genomics 2024Quote: ... Real-time PCR was conducted with cDNA as the template using the Quantitect SYBR Green PCR kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: Real-Time Quantitative PCR (RT-qPCR) was performed with the Rotor-Gene SYBR® Green PCR Kit (Qiagen), on a Rotor Gene-Q(R ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR amplification was carried out in 5 µl reactions using the QIAGEN Multiplex PCR Kit (QIAGEN, Germany). Each sample reaction contained 10–20 ng of genomic template DNA ...
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bioRxiv - Molecular Biology 2023Quote: 12-30 reactions were pooled and purified by PCR column purification using QIAquick PCR purification kit (Qiagen, 28104). DNA concentration was measured by Nanodrop 1000 (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... The amplification reaction was conducted using a Rotor-Gene-Q PCR Cycler Real-Time PCR system (Qiagen, Australia). The cycling parameters were as follows ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Size was validated by agarose gel electrophoresis and PCR products were purified using QiaQuick PCR Purification Kit (Qiagen). Equimolar amounts of purified DNA fragments (minimum 15fmol ...
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bioRxiv - Bioengineering 2023Quote: Isolated miRNA content was quantified by real time quantitative PCR (RT-qPCR) using the miScript PCR Kit (Qiagen) with a CFX Connect Real-Time system (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... PCR was conducted with the Mouse Inflammasomes (96-well format) RT2 Profiler PCR Array (Cat. # PAMM-097Z, Qiagen) according to the manufacturers protocol and was read on an Applied Biosystems® ViiA 7 Real-Time PCR System and analysis was conducted on QuantstudioTM Software by Applied Biosystems ...