Labshake search
Citations for Qiagen :
1051 - 1100 of 1359 citations for Chocolate Agar with Bacitracin for Haemophilus isolation 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Clones with DLK1 expression overlapping with isotype control antibody were expanded and used for DNA isolation using a MicroDNA kit (Qiagen, Germantown, MD). PCR was used to amplify the region surrounding the CRISPR cut site in the genomic DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... and spun at 14000rpm for 15 mins followed by the isolation of aqueous phase and isolating RNA using the RNAeasy mini kit (Qiagen, Catalog # 74014). cDNA synthesis was performed using the iScript cDNA synthesis kit (Cat# 170-7691) ...
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bioRxiv - Cancer Biology 2020Quote: ... The plate was loaded with a QIAgility Robot (Qiagen) and run on 7500 Fast Real-Time PCR System (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... Plates contained 5 µl of TCL lysis buffer (Qiagen) supplemented with 1%β-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... EasyXtal 15-well Tool crystal plates (Qiagen, Manchester, UK) were set up manually for HAdV-D15 ...
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bioRxiv - Biophysics 2024Quote: ... the plate was covered with AirPore tape sheets (Qiagen), and the plate was shaken at 250 RPM at 37 °C for 1 hour ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was isolated from tissue samples using the Qiagen RNeasy Plant Isolation kit according to the manufacturer’s protocol (Qiagen Inc., Valencia, CA, USA) and the RNA quality was checked using BioAnalyzer 2100 (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2020Quote: The 199 subsamples of colonies (henceforth “samples”) were dried before genomic DNA isolation using the DNeasy Blood and Tissue kit (QIAGEN, Germantown, MD, USA). Samples were homogenized in lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: Mitochondria were isolated from ~1.5 x 107 mitochondrial donor cells per mitochondrial transfer using the Qproteome Mitochondrial Isolation Kit (Qiagen, Hilden, Germany, Cat. #37612) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and wRB6) with or without shESRRG knockdown using a combination of TriZol and the RNeasy Mini RNA isolation kit (QIAGEN, Valencia, CA, USA). RNA-seq libraries were prepared using the TruSeq Stranded Total RNA prep kit with Ribo-Zero Gold to remove cytoplasmic and mitochondrial rRNA according to the manufacturer’s recommendation (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation was performed according to the manufacturer’s protocol (DNA Purification from Tissues) using the QiAamp DNA Mini Kit (Qiagen Sciences LLC, Germantown, MD). DNA was eluted after a 10 min incubation with 40 μL of Buffer AE ...
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bioRxiv - Immunology 2020Quote: ... washed and lysed with RLT buffer containing β-mercaptoethanol for RNA isolation as per manufacturer’s instructions with Qiagen RNAeasy Micro Kit (Qiagen, Germantown, MD, USA).
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from soil subsamples in triplicate and with negative control using the DNeasy PowerSoil DNA isolation kit (Qiagen, Germantown, MD, USA), according to the manufacturer’s instructions with one minor modification ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA from mock communities cultured in vitro was extracted using a plate-based adaptation of the above protocol including a bead beating process combined with phenol–chloroform isolation with QIAamp 96 DNA QIAcube HT kit (Qiagen Beverly LLC #51331) protocols43.
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bioRxiv - Physiology 2023Quote: Isolated human LGR5+ cells were stored at −80°C until processed for RNA isolation using RNeasy micro kit as per manufacturer instruction (Qiagen, Germantown, MD, USA). RNA processing ...
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bioRxiv - Molecular Biology 2023Quote: A magnetic bead extraction was used to extract and purify formerly adsorbed DNA in phosphate buffer using a version of Powersoil® DNA isolation protocol (Qiagen, Hilden, Germany) using homemade reagents (Sepulveda et al. ...
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bioRxiv - Molecular Biology 2023Quote: 109 conidia of the isolate Pt2 were subjected to gDNA extraction by using the PowerPlant® Pro DNA Isolation Kit (Qiagen, Hilden, Germany) (the manufacturer’s instructions were followed) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from fly hearts (15 per sample) in replicates of five with a RNeasy Isolation kit (QIAGEN, Germantown, MD, USA) per manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: Ileal samples were weighed and whole genomic DNA was extracted using the PowerFecal Pro® DNA Isolation Kit as per the manufacturer’s instructions (Qiagen, Germantown, MD, USA). 1 μl of each sample was used for 2-step real-time PCR amplification using SFB-specific primers (SFB736F ...
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bioRxiv - Microbiology 2024Quote: ... Isolation of genomic DNA from Sterivex filtered water samples were processed using the DNeasy PowerWater Sterivex Kit (Qiagen Cat no. 14600-50-NF) following manufacturer’s specifications ...
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bioRxiv - Plant Biology 2024Quote: ... DNA was purified from rhizosphere extracts and bulk soil using a QIAGEN DNeasy PowerSoil Pro DNA Isolation Kit (QIAGEN Inc., Germantown, MD, USA). Purified DNA was submitted to Integrated Microbiome Resource (Dalhousie University ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were processed in maximum one month from the isolation by using column purification method according to manufacturer’s instructions (QIAGEN RNeasy Micro Kit, 74004) and samples were stored at -80 °C until further processing.
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bioRxiv - Evolutionary Biology 2024Quote: ... then stored at -80°C until RNA isolation using the Qiagen RNeasy Mini kit according to the manufacturer’s recommendations (QIAGEN Sciences, Germantown, MD, USA). Each RNA sample represented the total RNA isolated from two pooled antennae of each individual ...
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bioRxiv - Microbiology 2021Quote: ... or the DNeasy® 96 Blood & Tissue plate kit (Qiagen) according to supplier’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... and loaded on RT2 PCR profiler plates (Qiagen, PAHS-078Z). Results were analyzed using the GeneGlobe Data Analysis Center (Qiagen).
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bioRxiv - Genomics 2020Quote: ... transfected cells were lysed on plate in RLT buffer (Qiagen) and stored at -80°C ...
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bioRxiv - Genomics 2024Quote: ... and mRNA was captured with a Turbocapture plate (Qiagen 72251). Reverse transcription and linear amplification were conducted directly on immobilised mRNA ...
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bioRxiv - Genomics 2020Quote: DNA was extracted from fecal samples and swabs using the Qiagen Powersoil® DNA Isolation Kit (Cat#12888-100) (QIAGEN Pty Ltd., Victoria, Australia) or the QIAamp Fast DNA Stool Mini Kit with MoBio bead shearing (Mo Bio ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was isolated from 20 female F1 pupae following a modified Qiagen Genomic Tip DNA Isolation kit (Qiagen Cat No. 10243 and 19060) protocol ...
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bioRxiv - Genomics 2020Quote: ... 80 µl of Viral Transport Media that had previously stored a nasopharyngeal swab from a patient infected with SARS-CoV-2 were used for RNA isolation using the QIAamp Viral RNA Mini spin kit (Qiagen, Cat No./ID: 52904) according to manufacturer specifications ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was isolated from 20 inbred male pupae following a modified Qiagen Genomic Tip DNA Isolation kit (Qiagen Cat No. 10243 and 19060) protocol ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from bulk HSPC samples (∼2 x 106 cells per sample) using a QIAGEN Blood & Tissue DNA isolation kit (QIAGEN, Inc., Germantown, MD, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated microvascular cells were stained for flow cytometric analysis or were additionally processed with myelin removal beads (Miltenyi 130-069-731) and 3 sequential positive selections with CD31 microbeads (Miltenyi 130-097-418) for RNA isolation (Qiagen RNeasy Micro Kit 74004). cDNA library preparation used Oligo-dT at 60 million clusters/sample (University of Chicago Genomics Facility).
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bioRxiv - Physiology 2023Quote: RNA was extracted from MLEC and lung tissue by lysis in RLT buffer containing 1% β-mercaptoethanol for RNA isolation and subsequently purified using the RNeasy Mini Kit (Qiagen, 74106, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: Young leaf tissue was collected from respective genotypes and subjected to isolation of genomic DNA following the procedure described in the Qiagen DNeasy® Plant Mini kit (250) (Qiagen Inc., Valencia, CA). Before library preparation ...
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bioRxiv - Genomics 2020Quote: ... and a protocol recommended by the manufacturer (User-Developed Protocol: Isolation of genomic DNA from plants and filamentous fungi using the QIAGEN® Genomic-tip, Qiagen Inc.). RNA was extracted from fungal isolates and from tobacco detached leaves infected with corresponding fungal isolates ...
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bioRxiv - Immunology 2021Quote: ... washed with PBS using a 96-well plate manifold base (Qiagen) connected to the vacuum and eluted into 96-well PCR plates using 86 μL of 0.1 M glycine-HCL buffer pH 2.7 ...
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bioRxiv - Immunology 2021Quote: ... washed with PBS using a 96-well plate manifold base (Qiagen) connected to the vacuum and eluted into 96-well PCR plates using 86 μl of 0.1 M glycine-HCL buffer pH 2.7 ...
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bioRxiv - Immunology 2020Quote: ... washed with PBS using a 96-well plate manifold base (Qiagen) connected to the vacuum ...
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bioRxiv - Immunology 2020Quote: ... washed with PBS using a 96-well plate manifold base (Qiagen) connected to the vacuum and eluted into 96-well PCR plates using 86 μl of 0.1 M glycine-HCL buffer pH 2.7 ...
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bioRxiv - Physiology 2022Quote: Cells were lysed directly in the plate using Buffer RLT (Qiagen) with 10uL/mL 2-mercaptoethanol following a quick PBS rinse ...
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bioRxiv - Microbiology 2023Quote: ... and a 96-well PowerMag Glass Bead plate (Qiagen, Hilden, Germany). The Glass Bead Sterilizer was turned on ...
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bioRxiv - Molecular Biology 2024Quote: ... washed with PBS using a 96-well plate manifold base (Qiagen) connected to the vacuum and eluted into 96-well PCR plates using 86 μl of 0.1 M glycine-HCl buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The plate was then processed in the PyroMark Q24 pyrosequencer (Qiagen). Results were analyzed with PyroMark Q24 Advanced 3.0.1 software ...
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bioRxiv - Immunology 2023Quote: ... RT qPCR was conducted with custom array plates (330171, Qiagen, Canada) using the LightCycler 480 Real-Time PCR system (Roche Molecular Systems Inc. ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from each plate using RNeasy mini kit (QIAgen) and QIAshredder following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 384 plates with SYBR green qPCR master mix (Qiagen LLC, 330523). Relative expression was measured using the ΔΔCt method with beta-actin.
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bioRxiv - Genetics 2024Quote: ... and final glucose plates using the DNeasy Blood & Tissue Kit (QIAGEN). We then amplified the chimeric acrIIA4 locus from the genomic DNA ...
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bioRxiv - Microbiology 2024Quote: ... for 5 minutes at 25 Hz with PowerBeads Pro plates (Qiagen). Following this ...
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bioRxiv - Molecular Biology 2020Quote: Cells were harvested from 24-well plates in 350μL RLT Plus (Qiagen) supplemented with 1% beta-mercaptoethanol after an 8-hour induction with 10nM E2 or DMSO ...