Labshake search
Citations for Qiagen :
951 - 1000 of 1359 citations for Chocolate Agar with Bacitracin for Haemophilus isolation 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... for 4 hours to inhibit reporter transgene expression (t4) prior to isolation of DNA and RNA using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen# 80004) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2021Quote: Samples were homogenized and nucleic acids were extracted using the MoBio RNA PowerSoil® Total RNA Isolation Kit (Qiagen, CA, USA) and the MoBio RNA PowerSoil® DNA Elution Accessory Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: Plasmid DNA isolation was performed on individual sewage pellets (420 mg) using Plasmid Purification Mini Kit (Qiagen, Cat No./ID: 12123) with QIAGEN-tip 100 (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... Microbial genomic DNA was extracted from nitrocellulose and acrodisc filters using the DNeasy PowerSoil DNA isolation Kit (QIAGEN, Germantown, MD USA) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... 250μL of homogenized stool from each sample were subsequently used for DNA extraction using a MoBio PowerMag Soil DNA isolation kit (QIAGEN, Germany) using the KingFisher Flex instrument ...
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bioRxiv - Cancer Biology 2022Quote: ... These specific colonies were amplified by culturing in liquid LB-medium with ampicillin (1:1000) followed by plasmid isolation using QIAprep Spin Miniprep Kit (Qiagen #27104), according to the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: CAL27-MK2WT and CAL27-MK2KD cells cultured in normoxia/hypoxia and tumors resected from the xenografted mice were employed for isolation of total cellular RNA using the RNeasy Mini kit (Qiagen, Germany) following the manufacturer’s recommended protocol (sample details are provided in Table 1) ...
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bioRxiv - Cancer Biology 2022Quote: ... Isolation of RNA and DNA from the same pellet was performed using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen # 80224) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated using the RecoverAll Total Nucleic Acid Isolation Kit (Thermo #AM1975) and concentrated using the RNeasy MinElute Cleanup Kit (Qiagen #74204). Library preparation and sequencing were performed by the Genome Sequencing Facility at St ...
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bioRxiv - Molecular Biology 2020Quote: The RNA isolation was performed with 50 µl of the crude extract on the QIAsymphony with the DSP Virus/Pathogen Kit (Qiagen, #937055). The RT-PCR was performed using 5 µl of 85 µl eluate with TIB MolBiol Lightmix® MODULAR SARS AND WUHAN CoV E-Gene Kit ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from cells with a combination of TriZol and RNeasy Mini RNA isolation kit (QIAGEN, Valencia, CA, USA), and reverse-transcribed using high capacity cDNA transcription kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... A small portion of the polyp was used for genomic DNA isolation using a DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany), and the remaining portion was immediately fixed in formalin and embedded in paraffin ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was then collected for the isolation and purification of the RNA using the RNeasy Mini Kit (QIAGEN, cat: 74104) according to manufacturer instructions ...
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bioRxiv - Genomics 2021Quote: ... miRNA of sixteen gonads (four samples each sex and treatment) was isolated by miRNAs isolation commercial kit (Qiagen® miRNA, 217004) and quality was assessed by BioAnalyzer (2100 Bioanalyzer ...
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bioRxiv - Cancer Biology 2020Quote: ... We fit the model taking into account both RNA isolation methods (Qiagen™ Trizol™ and Qiagen™ All Prep™) and stages of prostate cancer progression (BPH ...
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bioRxiv - Cancer Biology 2020Quote: ... We fit the model taking into account both RNA isolation methods (Qiagen™ Trizol™ and Qiagen™ All Prep™) and stages of prostate cancer progression (BPH ...
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bioRxiv - Cell Biology 2021Quote: ... Single cell clones were grown for immunoblot analysis and those showing loss of ATF4 protein were selected for sequence analysis involving the isolation of genomic DNA (Qiagen, 69504), PCR amplification using KOD Xtreme™ Hot Start DNA Polymerase (Millipore ...
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bioRxiv - Immunology 2021Quote: ... adherent cells were collected and either analyzed by FC or subjected to RNA isolation using the RNeasy Mini Kit (Qiagen #74106).
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bioRxiv - Microbiology 2021Quote: ... The RNA was precipitated in isopropanol and then subjected to additional purification using the RNeasy RNA isolation kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Total soil DNA extraction from 50 mg of soil samples was performed using the Qiagen DNeasy PowerSoil DNA Isolation Kit (Qiagen, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from GWSS and control samples (nGWSS = 79, ncontrol = 6) using a DNeasy PowerSoil DNA Isolation kit (Qiagen, Germany) with minor changes to the manufacturer’s protocol as follows ...
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bioRxiv - Microbiology 2023Quote: Samples were thawed on ice and 6-10 aliquots of approximately 0.25 g each were subjected to DNA extraction using the PowerLyzer® PowerSoil® DNA Isolation kit (QIAGEN) following the manufactureŕs instructions ...
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bioRxiv - Plant Biology 2023Quote: Five seeds from each Ningyou1 and Ningyou7 F1 cross were sown and leaf samples from each F1 plant were used for DNA isolation with the DNeasy® Plant kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was performed from 200 mg of three independent samples using the MO BIO’s Powersoil DNA isolation kit procedure (Qiagen 12888-100) as described previously (von Gastrow et al. ...
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bioRxiv - Genetics 2023Quote: Mutant libraries for each CRISPRi strain were grown in the absence and presence of IPTG followed by DNA isolation using the DNeasy Blood and Tissue Kit (Qiagen, Inc). Tn-Seq sample preparation ...
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bioRxiv - Immunology 2022Quote: RNA was extracted using the TRizol RNA isolation method (Cat. #15596026, Thermo-fisher Scientific, USA) combined with the RNeasy MinElute Cleanup (Cat. #74204, QIAGEN, Germany). SMARTseq v4 Ultra Low Input RNA kit for Sequencing (Cat ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from cryopreserved stool and colon biopsies using the PowerFecal DNA Isolation Kit and the company’s recommended protocol (Qiagen, Valencia, CA). Sequencing libraries were prepared as described by the Earth Microbiome Project [53] and sequenced on an Illumina MiSeq Sequencer (Illumina ...
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bioRxiv - Systems Biology 2024Quote: ... RH30 and HepG2 cells were treated as indicated in the figures before the isolation of RNA using the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA was also extracted from approximately 0.5g of ambient surface sediments (wet weight) by using the PowerSoil DNA Isolation Kit (Qiagen, Hilden, Germany). NanoDrop Lite (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... Genomic DNA from the pooled recombinants and parent strain were isolated using the Gentra® Puregene® DNA isolation kit (Qiagen) and sent for whole genome sequencing (BGI) ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from initial and exponential growth phase cultures grown on different N sources using a DNeasy PowerSoil Pro DNA isolation kit (QIAGEN, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... then 500 µL were used for DNA isolation by miniprep (Cat. No. 27104, QIAprep Spin Miniprep Kit, Qiagen, Germantown, MD, USA). The extracted DNA was quantified using the Qubit™ dsDNA BR Assay Kit (Cat ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and the pelleted cells were used for DNA isolation using the DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: RNA was isolated from hBSMC microtissues that were either untreated or treated with TGF-β1 and IL-13 for 7 days prior to RNA isolation using the RNeasy Mini Kit (Qiagen 74106). The RNA yield was measured using Agilent TapeStation (4200 TapeStation System ...
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bioRxiv - Developmental Biology 2023Quote: Single sorted cells (∼50’000 cells) were lysed in buffer RLT and stored at -80°C until starting the RNA isolation following manufacturer instructions (Qiagen, 74034). cDNA reaction was performed using qScript™ cDNA SuperMix (QuantaBio ...
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bioRxiv - Immunology 2022Quote: DNA isolation was performed from blood samples treated with EDTA according to our local protocol using QIAamp kits (Qiagen, Hilden, Germany). NFKB1 exons were amplified by PCR from gDNA and processed for Sanger sequencing according to standard protocols ...
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bioRxiv - Cell Biology 2024Quote: ... and the aqueous phase containing the RNA was transferred to a new tube for RNA isolation using the QIAcube System (Qiagen, #9001292) and RNeasy Mini Kit reagents (Qiagen ...
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bioRxiv - Genomics 2024Quote: Human bulk genomic DNA was extracted from 0.2 million chondrocytes collected from the same cartilage sample after single cell isolation using the QIAamp DNA Mini Kit (Qiagen 51304) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were directly lysed in ice-cold RNA isolater (Vazyme, R401) for total RNA isolation using RNeasy Mini Kit (Qiagen, 74004). Each group contains three replicates ...
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bioRxiv - Neuroscience 2024Quote: Sorted hMG were lysed in 300ul RLT buffer as supplied in the Qiagen RNeasy Kit and further processed for RNA isolation using the RNeasy Mini Kit (Qiagen 74104) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... was modified for rRNA depletion by replacing the mRNA isolation in the first segment of the protocol with ribo depletion using FastSelect rRNA (Qiagen 334387) as follows ...
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bioRxiv - Neuroscience 2024Quote: ... DNA and RNA co-isolation of mouse striatal punches were prepared with the AllPrep DNA/RNA 96 kit AllPrep DNA/RNA Kit (Qiagen, #80284) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... a buffy coat from a fresh 5-ml blood sample tube was aliquoted for peripheral blood mononuclear cells (PBMCs) isolation using RBC Lysis Solution (QIAGEN, 158904). The purified PBMCs were counted ...
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bioRxiv - Cell Biology 2024Quote: Brown adipocyte tissues were isolated from mice and subcellular fractionation was performed by following the Qproteome Mitochondria Isolation Kit instructions (Qiagen #37612).
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bioRxiv - Cell Biology 2024Quote: ... the supernatant was removed and pellet was collected to be processed for RNA isolation using RNeasy Plus Micro Kit (QIAGEN, 74034). cDNA was prepared using iScript™ cDNA Synthesis Kit (BIO-RAD ...
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bioRxiv - Bioengineering 2023Quote: ... total RNA was isolated from Hep3B cell spheroids on the pillar plate and in the ULA plate using a RNeasy Plus kit (Qiagen,74134). One μg of RNA was converted into cDNA using the high-capacity cDNA reverse transcription kit according to the manufacturer instructions (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: Cytokine- or vehicle-stimulated primary CD1 astrocyte cultures in 6-well plates and neuron cultures in 24-well plates were lysed and their total RNA extracted using RNeasy Mini Kits (Qiagen 74104) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were plated in 10 cm plates at 5 million cells/plate and the next day transiently transfected with the indicated plasmids using PolyFect (Qiagen 301105) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... which was further used for FACs sorting or were used for DNA isolation using the Blood and Tissue kit from Qiagen (Hilden, Germany) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... RNAlater was removed from the sorted cells prior to RNA isolation and total RNA was isolated from the pelleted cells using the RNeasy mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions with some modifications ...