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Citations for Qiagen :
851 - 900 of 1359 citations for Chocolate Agar with Bacitracin for Haemophilus isolation 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... from three brain regions (cerebral cortex, hippocampus, and cerebellum) of WT and Cul3+/- mice using the QIAGEN RNAeasy isolation kit (QIAGEN) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: Plasmid isolation from yeast was performed according to a user-developed protocol from Michael Jones (protocol PR04, Isolation of plasmid DNA from yeast, QIAGEN) using a QIAprep Spin Miniprep kit (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: ... from bacterial isolates or mock communities cultured in vitro was extracted using a plate-based protocol that included a bead beating process and combined phenol-chloroform isolation with Qiagen QIAamp 96 DNA QIAcube HT kit (Qiagen) procedures.
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bioRxiv - Neuroscience 2021Quote: Total brain RNA from 21 days old Itm2bD/D and Itm2bww/ rats (2 male and 2 females per each genotype) was extracted with RNeasy RNA Isolation kit (Qiagen). Standard RNA-Seq procedures and data analysis was performed by Genewiz following proprietary methods (https://cdn2.hubspot.net/hubfs/3478602/NGS/RNA-Seq/GENEWIZ_RNA-Seq_Technical_Specifications_US.pdf) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in either glutamine-containing or glutamine-free medium for 24 h and then used for total RNA isolation using the RNeasy kit (Qiagen). Sample preparation for analysis and processing of the arrays were performed using the protocol provided by Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA and/or enriched small RNA fractions were isolated from whole-tick samples using the miScript microRNA isolation kit according to the manufacturer’s instructions (QIAGEN, China). The quantity and integrity of the total RNA was assessed using an Agilent 2100 Bioanalyzer system (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from up to 250 mg of soil using the DNeasy® Powersoil® DNA Isolation kit (Qiagen) following the protocol provided ...
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bioRxiv - Genetics 2022Quote: Sequencing libraries of small RNAs including snoRNAs were made from the same total RNA isolation but using a miRNA-Seq (Qiagen) library prep kit with a modified size selection for up to 200-bp fragments ...
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bioRxiv - Physiology 2022Quote: RNA isolation was performed using Trizol (cat # 15596026, Invitorgen) and was purified using RNeasy Plus Mini Kit (cat # 774104, Qiagen) using the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... 2 throat swabs were collected with eNAT™ swabs for microbial DNA extraction using the PowerFecal DNA isolation kit (Qiagen), and in PBS for lactobacilli cultivation on MRS agar ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from approximately 10 g of sediments for each depth using PowerMax Soil DNA Isolation Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: c-miR isolations from blood serum were carried out using affinity column-based miRNeasy Serum/Plasma Advanced Kit (217204, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... All samples were then passed 3-5 times through a 26G needle prior to RNA isolation using the RNAeasy mini kit from Qiagen. RNA concentrations were estimated by absorbance measurement at 260 and 280 nm ...
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bioRxiv - Developmental Biology 2021Quote: ... CD31+ and CD31- populations from two yolk sacs were combined and processed for RNA isolation (QIAGEN RNeasy Micro Kit #74004), cDNA synthesis and qRT-qPCR as described below.
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bioRxiv - Molecular Biology 2020Quote: ... Centrifugation was performed for 10 min at 10.000 x g and the top aqueous phase was further collected for RNA isolation using the RNeasy Mini Kit (Qiagen) according to the manufactures’ instructions ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from all the 0.22 µm filter membranes was performed using the Power Water DNA isolation kit (Qiagen, USA) according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Genomics 2020Quote: RNA isolation for all the 248 human subjects were performed using QIAamp Viral RNA Mini Kit (Qiagen, Cat. No. 52906). The isolated RNA was subjected to qPCR for determining viral load by Ct values ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from each fecal containing tube using the Tissue and Blood DNA Isolation kit (Qiagen, Valencia, CA) following the manufacturer’s instructions with slight modifications ...
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bioRxiv - Microbiology 2021Quote: ... (1 μl) for 18 h and the RNA was extracted by using the mirCury cell and plant isolation kit (QIAGEN). A final number of 108 treated cells were lysed using 500 μL of glass beads and the 600 μL of the Lysis buffer provided with the kit ...
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bioRxiv - Microbiology 2020Quote: Quantitative polymerase chain reaction (qPCR) analyses were performed on the new DNA extracts using PowerSoil DNA Isolation Kit (12888-50, QIAGEN) to estimate the abundance of bacteria and archaea at different depths in the sediment core ...
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bioRxiv - Microbiology 2020Quote: The 16 h clindamycin-induced and uninduced seed cultures were cultivated and incubated in 1 ml of RNA Protect in the same manner as for RT-PCR Total RNA was extracted with an RNeasy RNA isolation kit (Qiagen). The isolated RNA was treated with DNaseI (0,1 U μl-1 ...
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bioRxiv - Cell Biology 2021Quote: ... 10∼12 ml cell culture medium was subjected to extracellular RNA isolation using the exoRNeasy Maxi kit (Qiagen, Cat# 77164) with minor modifications ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid and genomic DNA isolations were carried out with the QIAprep Spin Miniprep Kit and the DNeasy Blood & Tissue Kit (Qiagen), respectively.
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bioRxiv - Microbiology 2021Quote: ... and processed for sampling within 4 hours of collection using the MO BIO PowerSoil DNA Isolation Kit (QIAGEN; Germantown, MD). DNA in the samples was amplified via PCR with primers targeted to the V1-V3 region of the 16S rRNA ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from approximately 200-300 μl of fecal sample using the MoBio PowerSoil HTP 96 DNA isolation kit (formerly MoBio, now Qiagen) on the Eppendorf EpMotion 5075 automated pipetting system according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... shRNA expressing cells were isolated by fluorescence activated cell sorting at passage doubling 1 and 15 followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Sequencing libraries were prepared by subjecting 18 μg (1.2 μg x 15 reactions ...
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bioRxiv - Cancer Biology 2022Quote: SUZ12 isogenic M3 MPNST cells were treated with or without DNMT inhibitors (50 nM daily Decitabine or 4 μM single dose of GSK862) for 4 days followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Bisulfite sequencing was conducted by the IGO core facility at MSKCC ...
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bioRxiv - Genomics 2020Quote: ... The lysate supernatant was mixed with an equivalent volume of freshly prepared 70% ethanol and purified using the RNeasy RNA isolation Kit (Qiagen). mRNA was extracted from approximately 5 μg of the total RNA samples using Sera-Mag oligo-dT beads (GE Healthcare Life Sciences) ...
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bioRxiv - Immunology 2020Quote: ... Within these gates CD8+MR1-5-OP-RU- and CD8+MR1-5-OP-RU+ T cell populations were sorted directly into lysis buffer for RNA isolation (RNeasy Plus Micro kit, Qiagen). Purity of ungated sorted CD8+humMR1-5-OP-RU+ cells was between 91-99% (Fig ...
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bioRxiv - Genomics 2021Quote: ... cells were washed in ice-cold PBS and collected on ice for the isolation of total RNA using the RNeasy Plus Mini Kit (Qiagen) following the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2020Quote: Genome-wide cytosine methylation levels were established using the Illumina HumanMethylationEPIC BeadChip Array following isolation of genomic DNA from buccal cells DNA (DNeasy Blood & Tissue Kit (Qiagen). Multidimensional scaling (MDS ...
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bioRxiv - Microbiology 2021Quote: ... Total DNA and RNA were extracted using the PowerSoil DNA isolation kit and Rneasy PowerSoil total RNA kit (Qiagen, Germany), respectively ...
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bioRxiv - Microbiology 2020Quote: ... DNA for metagenomics analysis was isolated from 20 g (wet weight) sediment per sample with the PowerSoil DNA Isolation Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: Genomic DNA was isolated from a suspension of 1 x 106 PBMCs from each individual in the study using a DNA isolation kit (Qiagen). Genotyping was then performed using the Infinium CoreExome-24 (v1.3 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at low speed (~1000 RPM) and lysed with one mL RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen).
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bioRxiv - Synthetic Biology 2023Quote: ... Genomic DNA and plasmids were extracted using the Fast Pure Bacteria DNA isolation mini kit (Vazyme) and the QIAprep Spin Miniprep Kit (Qiagen), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... in each treatment group the sorted samples were processed for genomic DNA isolation using the QIAamp DNA blood maxi kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Sampled tissue punches were flash frozen on dry ice and store at -80 °C until RNA isolation by RNeasy Plus mini kit (Qiagen). RNA quality checking was completed with the Qubit fluorometer system (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Tissue and skeleton samples were then preserved in PowerSoil DNA Isolation kit (MoBio Laboratories, Carlsbad, CA; now Qiagen, Venlo, Netherlands) bead tubes ...
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bioRxiv - Microbiology 2023Quote: Bacterial and archaeal DNA were extracted using the PowerSoil DNA Isolation Kit (MoBio Laboratories, Carlsbad, CA; now Qiagen, Venlo, Netherlands). To select for the 16S rRNA V4 gene region ...
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bioRxiv - Microbiology 2023Quote: Total DNA for SCS_HM2 sediments (∼0.5 g) were extracted using the PowerSoil DNA Isolation Kit (Catalog No. 12888-50, Qiagen, Germantown, MD) following the user’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumor samples were transferred in RPMI media supplemented with antibiotics and stored in RNAse later until RNA isolation using the RNeasy Mini Kit (QIAGEN). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was resolved by 1.5 % agarose followed by excision and isolation within the size range of 300-600 bp using Gel Extraction Kit (Qiagen). NGS libraries were pooled and quantified by qPCR using NEBNext Library Quant Kit (NEB) ...
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bioRxiv - Physiology 2023Quote: ... total RNA from normal human or AIL patient neutrophils was isolated using RNeasy Total RNA Isolation Kit (Qiagen, GmBH, Germany)/ TRIzol reagent (Life technologies ...
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bioRxiv - Cell Biology 2023Quote: ... CD133+ fraction was directly eluted with either lysis buffer for immunoblotting or lysis buffer for RNA isolation using RNeasy Plus Micro Kit (74034; QIAGEN). Isolation protocol for total RNAs including small RNAs was used according to the manufacturer’s instruction with the RNeasy Plus Micro Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumour samples were fresh-frozen in liquid nitrogen and stored at -80°C for protein isolation or preserved in RNAlater solution (Qiagen) for RNA isolation ...
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bioRxiv - Cell Biology 2022Quote: ... For each experimental condition total RNA was prepared from 5 OD600 units of cells using the RNeasy Plus RNA Isolation Kit (Qiagen). The synthesis of cDNA was performed using 500 ng of prepared RNA ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA from 5-20 mg of melanoma primary and metastatic tissues was extracted using AllPrep DNA/RNA Mini isolation kit (Qiagen). A total of 100 ng of total RNA was used as input for RNA- seq libraries using SMARTer Stranded Total RNA-seq - Pico input (Takara Bio USA ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA isolation from 200 mg of grounded tissue was performed with the RNeasy plant mini kit (Qiagen, Hilden, Germany) and the cDNA synthesis with the First Strand cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... a pool of 15 million cells from each condition were harvested for genomic DNA (gDNA) isolation using the DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s protocol.