Labshake search
Citations for Qiagen :
1001 - 1050 of 1359 citations for Chocolate Agar with Bacitracin for Haemophilus isolation 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was isolated from 100 to 300 µl nematode pellets using the Blood and Tissue DNA isolation kit (cat#69506, QIAGEN, Valencia, CA) following established protocols70 ...
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bioRxiv - Neuroscience 2021Quote: ... Lysis buffer was immediately added to CD11b+ cells for isolation of total RNA with the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany); alternatively ...
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bioRxiv - Molecular Biology 2021Quote: ... We collected 20 CA1 dissectates in one isolation cap (Molecular Machines and Industries GmbH, Eching, Germany) before adding RLT lysis buffer (AllPrep Kit, Qiagen, Hilden, Germany). Samples from one individual were collected the same day ...
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bioRxiv - Cell Biology 2022Quote: ... DNA samples were extracted from PKS skin fibroblasts and normal control skin fibroblasts using the Puregene DNA isolation kit (Qiagen, Valencia, CA) with modified protocol ...
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bioRxiv - Genomics 2020Quote: ... We tested four different extraction methods: (a) the PowerSoil® DNA Isolation Kit (12888-50, CAMBIO, UK, now 12888-100, DNeasy PowerSoil, QIAGEN LTD), (b ...
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bioRxiv - Genomics 2020Quote: ... and a protocol recommended by the manufacturer (User-Developed Protocol: Isolation of genomic DNA from plants and filamentous fungi using the QIAGEN® Genomic-tip, Qiagen Inc.). RNA was extracted from fungal isolates and from tobacco detached leaves infected with corresponding fungal isolates ...
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bioRxiv - Cancer Biology 2021Quote: ... For RNA isolation tumor tissue was lysed using Qiazol and total RNA was isolated using the miRNeasy Mini Kit (Qiagen, Cat.No# 74004) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... the mEV solution was incubated with 100 µl of precipitation buffer B from the Qiagen miRCURY Exosome Isolation Kit (Qiagen, Germantown, MD) overnight (∼12 hours ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from 3 ml of each raw milk sample using the PowerFood™ Microbial DNA Isolation Kit (MoBio Laboratories, Qiagen, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA isolation from cloacal swabs and intestinal contents (approximately 200 mg) was performed using QIAamp PowerFecal DNA Kits (Qiagen, Venlo, Netherlands). For cloacal swabs ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from approximately 500 μL (wet weight) of each sample using QIAamp PowerFecal DNA Isolation kits (Qiagen, Hilden, Germany), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted following the double extraction protocol: RNA isolation by TRIzol followed by a Qiagen RNeasy Micro clean-up procedure (Qiagen, Hilden, Germany). The extracted RNA samples were inspected on Agilent Bioanalyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA from Escherichia coli MG1655 was isolated using the PowerLyzer® UltraClean® Microbial DNA Isolation Kit from Qiagen (Hilden, Germany). Bacterial cells were harvested and lysed using glass microbeads ...
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bioRxiv - Microbiology 2021Quote: ... 4×106 HEK293T cells or 2×106 HeLa cells seeded in a 100 mm dish were transfected with 10μg of each plasmid purified using the endotoxin-free plasmid isolation kit (Qiagen, Germantown, MD, USA) with TransIT-293 (Mirus ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: Cells were lysed in RLT PLUS buffer and stored at -80°C until RNA isolation with the RNeasy Micro Kit (Qiagen, Hilden, Germany). cDNA was generated using the iScript Advanced cDNA generation kit (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... Islets were then picked and washed in PBS before lysis and isolation of whole RNA using the Qiagen RNeasy® Kit (Qiagen; #74106) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Two PowerBead preparations of 0.5 g were used along with the PowerSoil Pro DNA Isolation kit (MoBio Labs/QIAGEN, Carlsbad, CA, USA) according to the instructions ...
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bioRxiv - Cancer Biology 2021Quote: DNA was isolated from 10-20 8-μm frozen tissue sections of biopsies from humanized mice using the PureGene DNA isolation kit (Qiagen, Hilden, Germany). PCR was performed with six framework region 1 subgroup specific primers and a JH primer mix (3’ JH mix ...
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bioRxiv - Cancer Biology 2021Quote: Snap frozen primary tumors were prepared for RNA isolation by TRIZOL digestion and subsequent homogenization using a TissueLyser II (Qiagen, Hilden, Germany) with a 5 mm stainless steel bead for 2 min at 30 Hz ...
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bioRxiv - Microbiology 2021Quote: ... Viral nucleic acids were extracted from 140 μl of the virus transport medium derived from the nasopharyngeal swab using the viral RNA isolation kit according to the manufacturer’s protocol (Qiagen, Venlo, The Netherlands). For the 3′ RACE ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from cultures of LSUCC0096 that had reached max cell density (~106 cells mL−1) growing in JW1 medium (10) at room temperature using a MoBio PowerWater DNA Isolation kit (QIAGEN, Massachusetts, USA) following the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... Samples were centrifuged at 14,000 g at 4°C for 10 mins and the supernatant collected for processing using a RNeasy mini-isolation kit (Qiagen Ltd, UK) according to the manufacturers’ protocol ...
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bioRxiv - Cell Biology 2020Quote: ... coli cells were cultured at 37°C and plasmid isolation was carried out using a Qiagen Plasmid Mini Kit (Qiagen, Germantown, MD) according to manufacturer instructions ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was isolated from 50 to 300 µl nematode pellets using the Blood and Tissue DNA isolation kit (cat# 69506, QIAGEN, Valencia, CA) following established protocols (Cook et al ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was isolated from 100 to 300 µl nematode pellets using the Blood and Tissue DNA isolation kit (cat# 69506, QIAGEN, Valencia, CA) following established protocols (Cook et al ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was extracted from the tumor specimens and the cultured cell lines using a QIAmp mini DNA isolation kit (Qiagen, cat # 51304). Sixteen loci were screened for regions of microsatellite instability with defined trinucleotide or tetranucleotide repeats within the chromosomes ...
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bioRxiv - Microbiology 2022Quote: DNA was isolated from the swab and tissue samples using a Qiagen PowerSoil DNA Isolation kit (Qiagen, Hilden, Germany; cat. # 12888-100) following the manufacturer’s protocol (23) ...
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bioRxiv - Microbiology 2022Quote: ... and the sediments were used to coextract the total RNA and DNA using the RNeasy PowerSoil Total RNA isolation kit and the DNA elution kit according to the manufacturer’s protocol (Qiagen®, Valencia, CA). To eliminate potential contaminant DNA in RNA samples ...
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bioRxiv - Neuroscience 2023Quote: ... Colonies were selected and grown in liquid broth (Fisher, catalog #12780052) overnight at 37 °C for DNA isolation (by QIAprep Spin Miniprep Kit, Qiagen, catalog #27106) and sequence verification (EuroFins Genomics).
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bioRxiv - Plant Biology 2024Quote: ... 100⍰mg of ground powder from each tissue was used for RNA isolation using the RNeasy Plant Mini Kit (#74904, QIAGEN Ltd UK) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The pellets were resuspended with OMV buffer and re-pelleted again by centrifugation at 100,000 g (31,000 RPM) for 2 h at 4°C and lysed with Qiazol followed by RNA isolation with the miRNeasy kit (Qiagen, Germantown, MD) to obtain total RNA including the small RNA fraction ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA isolation was performed using QIAzol Lysis Reagent and the miRNEasy Mini Kit including the RNAse-free DNase Set (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The soil samples from the rhizosphere and the root tissue samples for the root endosphere were processed for the downstream DNA extraction procedure using the Qiagen PowerSoil® DNA Isolation Kit (Qiagen, USA). The extracted DNA samples were analyzed for quality and quantity using NanoDrop 1000 (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from 250mg edm for the 246 samples (compositional variants and original soils) using the DNeasy PowerSoil-htp 96 well DNA isolation kit (Qiagen, Hilden, Germany). Generation of amplicons for Illumina MiSeq sequencing was done according to a two steps PCR protocol as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... All nucleic acid isolation from tissue sections was performed using a QIAcube automated sample preparation system according to the manufacturer’s instructions (Qiagen Inc., Valencia, CA). Sections were processed in random batches of 12 on the QIAcube to avoid confounding section number with potential technical sources of variation associated with nucleic acid isolation.
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bioRxiv - Plant Biology 2024Quote: The isolation of total RNA from the collected samples was performed with the use of the RNeasy Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... tissue was snap frozen in liquid nitrogen and pulverized with a microdismembrator S (B. Braun Biotech, Melsungen, Germany) before total RNA isolation by means of the Lipid Tissue Mini Kit (Qiagen, Hilden, Germany). Isolated cells were lysed in RLT buffer ...
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bioRxiv - Microbiology 2023Quote: ... each Sterivex filter membrane was sectioned in half (representing approximately 500 ml of sample) and placed in Qiagen DNeasy Powersoil DNA isolation kit bead-beating tubes (Qiagen; Hilden, Germany) for immediate DNA extraction and the other half for storage at -80 °C ...
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bioRxiv - Microbiology 2023Quote: ... Each saliva sample was vortexed and 1) 500-750 μl was pipetted into a Powersoil Bead Tube (MOBIO PowerSoil DNA Isolation Kit, QIAGEN Cat #12888) for DNA extraction within two hours of collection (fresh) ...
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bioRxiv - Microbiology 2023Quote: ... total DNA extractions were performed on select Lehman Caves samples using the DNeasy Powersoil Pro DNA Isolation Kit (Qiagen, Germantown, MD, USA), DNeasy PowerMax Soil Kit ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of approximately 0.2 g of biomass pellets were further used for mDNA extraction also with the PowerLyzer® PowerSoil® DNA Isolation kit (QIAGEN).
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bioRxiv - Cancer Biology 2023Quote: ... RNA sequencing on the pancreatic xenografts in the orthotopic mouse model using PDAC cells (AsPC-1 ± SERPINB3) was also performed following isolation of RNA with the RNeasy Plus Mini Kit (QIAGEN, Venlo, Netherlands). Sequencing was performed on NovaSeq 6000 SP (Illumina ...
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bioRxiv - Bioengineering 2023Quote: ... which were cultivated on a rotary shaker at 37° for 6 h before plasmid isolation using a Midi Kit (Qiagen, Hilden, Germany). The obtained plasmid libraries were subsequently used for electroporation of C ...
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bioRxiv - Microbiology 2023Quote: ... we placed filters in 2 mL tubes with beads and 1 mL TE buffer (Qiagen plasmid isolation kit, Qiagen Germantown, MD, USA) and ran them in the tissue lyser at either ½ speed or max speed for 30 sec or 1 min ...
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bioRxiv - Developmental Biology 2023Quote: ... stagnalis postmetamorphic snails (st. 28-29) and adult nervous systems were utilized for total RNA isolation using the RNeasy Mini Kit (Qiagen, Hilden, Germany) in accordance with the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: Total soil DNA was extracted from 0.25 g of rhizosphere soil samples using the Power Soil DNA Isolation Kit (Qiagen, Calsbad, CA, USA). The NanoDrop 1000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... An equal volume of inoculum from each pool was then combined and used as starter culture for a single maxiprep plasmid isolation of the pooled HDR template library (Qiagen Maxiprep kit).
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bioRxiv - Neuroscience 2024Quote: ... Pooled plasmids were transformed into Endura electrocompetent cells (LGC, Middlesex, UK) and plasmid isolation performed using Qiagen Giga Prep Endofree kits (QIAGEN, Germantown, MA). For the second round library generation ...
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bioRxiv - Microbiology 2024Quote: ... DNA was isolated from the lower non-aqueous layers from the samples used for RNA isolation using Qiagen PureGene Core Kit A kit (Qiagen, #158667, USA) following manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The total RNA from the lysed chondrocytes was isolated and purified using an RNeasy Mini RNA isolation kit from Qiagen (Hilden, Germany). The RNA obtained was quantified using the NanoDrop from ThermoFisher (Massachusetts ...