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Citations for Qiagen :
51 - 100 of 5860 citations for rno mir 542 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Specimens were incubated for 24 hours at room temperature in the presence of either an ASO antimiR targeting hsa-miR-134-5p (‘Ant-134’; Qiagen, Manchester ...
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bioRxiv - Genetics 2022Quote: ... Routine qPCR for miR detection and validation (see Supplementary Table 1 for miR sequences) was performed with the miScript PCR control set (catalog number 218380; Qiagen, Germany). The miScript™ miRNA PCR Array Human Serum & Plasma 384HC (Cat No ...
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bioRxiv - Developmental Biology 2021Quote: ... using primers amplifying miR let7 (Qiagen, MS00005866; let-7g Qiagen, MS00010983).
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bioRxiv - Developmental Biology 2021Quote: ... using primers amplifying miR let7 (Qiagen, MS00005866; let-7g Qiagen, MS00010983).
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bioRxiv - Microbiology 2019Quote: ... Cementing PCR was performed using primer set 4438/441 and cleaned up using a QIAquick PCR Purification Kit (Qiagen) and transformed into DK1042.
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bioRxiv - Neuroscience 2021Quote: ... Validated primer sets were obtained from Qiagen.
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bioRxiv - Neuroscience 2024Quote: ... Validated QuantiTect primer sets obtained from (Qiagen) for IL-1α (QT00001127) ...
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bioRxiv - Microbiology 2020Quote: ... The triplicate PCR amplicons from each sample-primer set combination were then pooled and purified using the QIAquick PCR purification kit (Qiagen), with a slightly altered protocol in which the final elution was performed on nuclease-free water pre-heated to 55°C and incubated for 5 min at 55° C before the final centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... All reactions were set up in triplicate using a One-Step RT-PCR kit (Qiagen, UK) as per the following run conditions ...
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bioRxiv - Cancer Biology 2023Quote: 1×104 SiHa or 1×103 Hela cells/6-well were seeded and transfected with pSG5-miR-142-5p expression vector or pSG5 empty vector as a control using PolyFect Transfection Reagent (Qiagen, Hilden, Germany). After 48 h ...
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bioRxiv - Cancer Biology 2022Quote: ... according to manufacturer’s protocol (MiScript Primer assays and II RT kit for cDNA synthesis and MiScript SYBR Green PCR Kit for RT-qPCR, 218161, Qiagen) from which the recovery of cel-miR-39 spike-in control was confirmed.
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bioRxiv - Developmental Biology 2020Quote: ... and quantitative RT-PCR was performed using SYBR-based primers (Supplemental Table 1) and RT SYBR Green qPCR Mastermix (Qiagen, #330509) on a CFX96 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... and miR-42-3p were obtained from the miScript Primer Assays kit (Qiagen). miR-47-3p was used as the endogenous control ...
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bioRxiv - Developmental Biology 2019Quote: ... Predesigned primers for miR-126 and U6 were purchased from Qiagen (cat # 218300).
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bioRxiv - Developmental Biology 2020Quote: ... RT-PCR was performed using OneStep RT-PCR Kit (QIAGEN) using the following primers ...
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bioRxiv - Immunology 2023Quote: ... was performed in duplicates using predesigned primer sets (Quantitect Primer Assays, Qiagen, Germantown, MD). Relative mRNA expression between groups was analyzed by the delta-delta Ct method and normalized to housekeeping genes ...
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bioRxiv - Cell Biology 2019Quote: ... qPCR was performed on a LightCycler 96 Real-Time PCR System using SYBR Green RT-PCR with the following Quantitec primers from Qiagen; IL-6 (QT0009887) ...
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bioRxiv - Immunology 2023Quote: ... cDNAs were synthesized using High Capacity RNA-to-cDNA kit (ThermoFischer Scientific) and quantitative RT-PCR were carried out using specific primers (Table S2) and SYBR Green PCR Master Mix (Qiagen). PCR amplification of Gapdh was performed to control for sample loading and normalization between samples ...
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bioRxiv - Microbiology 2020Quote: ... CDNA fragments were amplified with strain-specific primers using the one step RT-PCR kit (Qiagen). Sequencing was performed by Macrogen Europe ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μM barcode LNA RT primer (Qiagen), 1U/μL RiboLock RNase inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... miR-29b and miR-29c (Qiagen), using Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... miRNA-21-5p (Qiagen #YP00204230), Let-7b-5p (Qiagen #YP00204750) ...
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bioRxiv - Cancer Biology 2021Quote: ... Let-7b-5p (Qiagen #YP00204750), miRNA 22-3p (Qiagen #YP00204606) ...
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bioRxiv - Plant Biology 2020Quote: ... RT-PCR was performed using the OneStep RT-PCR kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR was performed using One Step RT-PCR kits (Qiagen) and a mixture of TRAV- ...
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bioRxiv - Plant Biology 2024Quote: ... RT-PCR was conducted using a OneStep RT-PCR kit (Qiagen) with the following primer pair ...
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bioRxiv - Biochemistry 2020Quote: ... Reverse ttranscription (RT) for miRs was completed with 300 ng of total RNA using a miScript II RT Kit (Qiagen) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: RT-qPCR was performed with 0.4 μM primers and using the QuantiTect SYBR Green PCR mix (Qiagen). Primer sequences are indicated in Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative RT-PCR was performed using QuantiTect Probe RT-PCR Kit (Qiagen) in a StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... RT-PCR reactions were prepared using QuantiTect Probe RT-PCR Kit (Qiagen), with final concentrations of primers and probe used were 400 nM and 200 nM respectively ...
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bioRxiv - Microbiology 2020Quote: ... rt-PCR was conducted with a OneStep RT-PCR kit (Qiagen Canada) by following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: RT-PCRs were performed with the One-step RT-PCR kit (QIAGEN) with SAO00187/SAO00188 (for 12S rRNA) ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR was performed using the OneStep RT-PCR Kit (Qiagen, #210212), with the following conditions ...
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bioRxiv - Cell Biology 2021Quote: ... RT-PCR was performed by QuantiTect SYBR Green RT-PCR kit (Qiagen) using the following primer ...
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bioRxiv - Microbiology 2022Quote: Nested RT-PCRs were performed using the OneStep RT-PCR kit (Qiagen). In each reaction ...
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bioRxiv - Cancer Biology 2022Quote: ... Cel-miR-39 miR mimic (MS00019789, Qiagen) was added to each sample to serve as a spike-in control for monitoring the miR purification and amplification ...
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bioRxiv - Cancer Biology 2023Quote: ... miR-340 or miR-497 (339306, Qiagen) following the manufacturer’s instruction with Lipofectamine RNAiMax (13778075 ...
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bioRxiv - Microbiology 2020Quote: ... and RotorGene Q (Qiagen)-OneStep RT-PCR (Qiagen) or QuantiNova Probe RT-PCR kits ...
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bioRxiv - Cancer Biology 2020Quote: ... and miScript Primer Assays (control/normalization:HS-RNU6-2_11, MIR141:HS-miR-141_1, MIR200A:HS-miR-200a_1, MIR200B:HS-miR-200b_3, MIR200C:HSmiR200c_1, Qiagen). Samples ran on a QuantStudio 12K Flex qPCR system (Life Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... Let-7g miR analysis was performed by using Mm_Let-7g_2 miScript Primer Assay (Qiagen, MS00010983) and RNU6-2 (Hs_RNU6-2_11 miScript Primer Assay ...
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bioRxiv - Molecular Biology 2021Quote: ... miRCURY LNATM miRNA inhibitor for anti-miR20a-5p and anti-miR149-5p were bought from Qiagen. Total RNAs were extracted 24 hours or 96 hours (for anti-miR test ...
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bioRxiv - Microbiology 2019Quote: ... The HA and NA influenza segments (complete or fragments) were amplified from cDNA template using subtype-specific set of primers and purified with QIAquick PCR Purification kit (Qiagen). Nucleotide sequences of viral genes were determined using BrilliantDye™v3.1 Terminal Cycle Sequencing Kit (Nimagen) ...
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bioRxiv - Molecular Biology 2020Quote: cDNA synthesis of purified miR-206 was carried out using miRCURY LNA RT Kit (Qiagen) as described ...
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bioRxiv - Genetics 2020Quote: ... reverse primers 852Rb-AGGAAGATAGAGAAAGAGCAACC and 852Rc-AGGAAGATAGAAAAGGAGCAACC using QIAGEN One-Step RT-PCR kit (QIAGEN GmbH, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The RT-PCR was performed using Qiagen One-Step RT-PCR kit (Qiagen) master mix ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was performed using a One-Step RT-PCR kit (Qiagen, #210210) using the following primers Forward ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Quantitative RT-PCR was performed using Quanti Tect Probe RT-PCR Kit (Qiagen) in a StepOne Plus™ Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... and RT-PCR carried out using the One-Step RT-PCR kit (Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR was performed to amplify microRNA 223-3p using the primer hsa-miR-223-3p miRCURY LNA miRNA PCR Assay (Qiagen, #339306, gene Globe ID: YP00205986) and LNA SYBR green PCR kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... miR 155 and miR 224 (Qiagen, HL, Germany) (with sequences as given in supplementary Table S1 ...