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Citations for Qiagen :
401 - 450 of 5860 citations for rno mir 542 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Quantitative RT-qPCR was performed using QuantiTect SYBR®-Green PCR kits (Qiagen) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was conducted by QuantiTect SYBR® Green PCR Kit(204145, QIAGEN) approaches on Agilent MP3005P thermocycler ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-qPCR was performed using a QuantiTect SYBR® Green PCR kit (Qiagen) and primers for the target genes (Table 2) ...
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was analyzed using the QuantiTect SYBR Green RT-PCR kit (Qiagen) in a LightCycler 480 (Roche ...
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bioRxiv - Evolutionary Biology 2022Quote: ... QuantiTect Probe RT-PCR (reverse transcription polymerase chain reaction) Kit (QIAGEN, Hilden, Germany) was used to perform RRT-PCR reactions in the ABI 7300 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Bioengineering 2023Quote: ... and analyzed using the RT² Profiler PCR Data Analysis Tool on GeneGlobe (Qiagen). For reverse transcription ...
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bioRxiv - Biophysics 2023Quote: ... We assembled qPCR reactions with QuantiFast SYBR Green RT-PCR Kit (Qiagen # 204156), gene-specific oligonucleotides (Supplementary Table 14 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Expression of AmTyr1 was quantified using QuantiFAST SYBR Green RT-PCR kit (QIAGEN) on Applied Biosystem 7900 cycler (ASU DNA Facilities ...
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bioRxiv - Neuroscience 2020Quote: ... PCR assays were performed based on previous published method (Gonatopoulos-Pournatzis et al., 2018) OneStep RT-PCR kit (QIAGEN) was used according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2022Quote: ... cDNA of each gene target was reverse-transcribed and PCR amplified with the PyroMark OneStep RT-PCR kit (QIAGEN) using one biotinylated (reverse ...
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bioRxiv - Cancer Biology 2019Quote: ... Real time one step qRT-PCR was carried out using the QuantiTect SYBR® Green RT-PCR Kit (Qiagen) according to manufacturer’s instructions before analysis on the 7900 PCR machine (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... qRT-PCR to quantify cap and gyr mRNA was performed using the QuantiFast SYBR-Green RT-PCR kit (Qiagen). Standard curves were generated using 10-fold serial dilutions (104 to 108 copies ...
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bioRxiv - Microbiology 2023Quote: ... SYBR green real-time PCR assay was carried out in a 20μL PCR mixture volume consisting of 10 μL of 2X Quantitect SYBR green RT-PCR Master Mix (Qiagen) containing HotStarTaq DNA polymerase ...
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bioRxiv - Microbiology 2020Quote: The uniplex RT-qPCR assays to detect DENV were performed with the QuantiTect Probe RT-PCR Kit (Cat. 204445, Qiagen, Hilden, Germany), and the amplification of RT-PCR was performed with Superscript III Platinum One-Step reagents RT-qPCR System (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 ng of each sample was used per reverse transcription-quantitative polymerase chain reaction (RT-qPCR) reaction using the QuantiTect SYBR® Green RT-PCR Kit (Qiagen, 204243) on Rotor-Gene Q (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... Oligonucleotides containing shRNA inserts were PCR-amplified with primers 5’-TCTCGAATTCTAGCCCCTTGAAGTCCGAGGCAGTAGGC-3’ and 5’-TGAACTCGAGAAGGTATATTGCTGTTGACAGTGAGCG-3’ and purified with QIAquick PCR Purification Kit (Qiagen). shRNA inserts and miRE18_LT3GEPIR_Ren714 backbone (inducible via Tet-On system ...
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bioRxiv - Genetics 2020Quote: ... Analyses of HTT CAG repeat size in both HttQ111 mice and in patient fibroblasts was performed by PCR using human-specific HTT primers and Taq PCR Core Kit with Q solution (Qiagen), as previously described (17 ...
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bioRxiv - Immunology 2021Quote: ... Each AES was then amplified by PCR on a 3 kb fragment using primer X5 and X6 and purified using QIAquick PCR Purification kit (Qiagen). For gene yrbE4A ...
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bioRxiv - Microbiology 2020Quote: ... templates containing a T7 phage promoter sequence (5’-TAATACGACTCACTATAGGG-3’) were amplified by PCR using corresponding primer pairs and were purified with a QIAquick PCR purification kit (Qiagen). The primers used are listed in S1 Table ...
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bioRxiv - Molecular Biology 2022Quote: ... We designed species specific MALAT1 tiling primers for human and green monkey (Table S1) and performed multiplex PCRs following Qiagen Multiplex PCR protocol (Qiagen). cDNA was divided equally between primer sets ...
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bioRxiv - Cancer Biology 2023Quote: ... Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen, Germany). 2-nd PCR (PCR 2 ...
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bioRxiv - Immunology 2023Quote: ... The first round of PCR was carried out using the ImmunoSEQ proprietary PCR primer mix (32 μL per sample containing 25 uL of QIAGEN 2× Multiplex PCR Master Mix ...
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bioRxiv - Developmental Biology 2021Quote: ... MicroRNA qRT-PCR was carried out with custom designed primers to conserved miRNAs (Qiagen) and custom primers from IDT were used to quantify axolotl mRNAs:
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bioRxiv - Physiology 2020Quote: ... or the QuantiTect PCR Kit with self-designed primers for neuronal factors (Qiagen, Germany) (Supplementary Table 3) ...
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bioRxiv - Microbiology 2020Quote: Bact27F and Bact1492R primers either by directly sequencing agarose-gel purified PCR products (Qiagen), or TA cloning (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Quantitative real time-PCR was performed with specific primers from QIAGEN (Valencia, CA, USA) and detection reagent SYBR Green mix (QIAGEN ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene-specific primers were used for end-point PCR (HotStarTaq Plus DNA Polymerase; Qiagen), to detect inclusion or exclusion of alternative exons ...
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bioRxiv - Physiology 2021Quote: ... Samples were spiked using Cel-miR-39-3p (Qiagen, 219600) and MS2 carrier RNA (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were transfected with miR-22m (Qiagen, Hilden, Germany) or miR-22i (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... To confirm the expression of miR-130b-p5 (Qiagen, MS00001554) and −3p (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... The assay for MBP was tested for its sensitivity using the EpiTect PCR Control DNA Set (Qiagen). Product amplification was performed using the following reaction mixture ...
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bioRxiv - Microbiology 2020Quote: ... Positive sense vRNA and GAPDH transcripts were measured by qRT-PCR using the QuantiTect SYBR Green RT-PCR kit (Qiagen) on an Applied Biosystems 7300 machine ...
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bioRxiv - Physiology 2020Quote: ... ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen) on an iCycler instrument (BioRad) ...
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bioRxiv - Immunology 2019Quote: Total RNA samples were diluted to a concentration of 10 ng/μl prior to gene expression studies by qRT-PCR using QuantiFast SYBR green RT-PCR kit (Qiagen) in 7900HT Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... The remaining 18 μl of RT-PCR DNA was purified by a QIAquick PCR Purification kit (Qiagen, Germantown, MD, USA). Sequences of the purified RT-PCR products were generated using a BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... One-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) following the standard protocol for a QuantStudio 7 Flex RT-PCR system ...
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bioRxiv - Microbiology 2021Quote: ... RNA was reverse transcribed and viral genome copies quantified by real-time PCR using the OneStep RT-PCR Kit (Qiagen) and the StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... Synthetized cDNA was subjected to a PCR array specific for the human antiviral response (RT² Profiler PCR array – PAHS-122Z, SA Biosciences, Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen) on an iCycler instrument (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... we used both one-step RT-PCR and two-step (cDNA synthesis followed by separate PCR amplification) reaction designs with commercial One-Step Ahead RT-PCR Kit (Qiagen) or IEH in-house RT ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR mixtures were assembled using 50 ng or 5 µl total RNA and QuantiTect Probe RT-PCR Kit (Qiagen). Amplification and analysis were performed using QuantStudio3 system (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... The PNPLA2 transcript levels in ARPE19 cells determined by quantitative RT-PCR were normalized using the QuantiTect SYBR Green PCR Kit (Qiagen) in the QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time PCR was performed with 100ng of total RNA using the QuantiFast SYBR Green RT-PCR kit (Qiagen, Singapore) detector system (ABI Quant studio 5 ...
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bioRxiv - Cancer Biology 2020Quote: Quantitative real-time RT-PCR (qRT-PCR) amplification was performed on RNA extracted from cells using RNeasy Mini kit (#74104, Qiagen). RNA (50 ng ...
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bioRxiv - Neuroscience 2020Quote: ... The quantitative real-time RT-PCR (qPCR) analysis for Bcl2l1 and Bcl2l11 was performed using a SYBR Green PCR Mix (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Real-time quantitative RT-PCR analysis of the samples was carried out using the mouse antiviral response RT2 Profiler PCR array (Qiagen) on a 7900HT Fast Real-Time PCR system (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... a single GFP+ or GFP-J-Lat 11.1 cell was sorted directly into each well of a 96-well PCR plate containing the pre-amplification mastermix consisting of 1 μL One-step RT-PCR enzyme (QIAGEN), 5 μL 5× One-step RT-PCR buffer (QIAGEN) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 75 ng of rRNA was reverse transcribed and amplified with 8 PCR cycles using the OneStep RT-PCR kit (Qiagen) and primers MS2_quant_F and MS2_quant_R (Supplemental Table 5) ...
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bioRxiv - Cell Biology 2020Quote: ... and run on the RT^2 First Strand Kit (Stem cell PCR array) (Qiagen). The array was run in triplicate (N=3 ...
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bioRxiv - Physiology 2019Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...