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Citations for Qiagen :
301 - 350 of 5860 citations for rno mir 542 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 12.5 µL of 2X Quantitect Probe RT-PCR Master Mix (Qiagen), 0.5 µL of Superscript III Reverse Transcriptase (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was synthesized using an RT kit for qRT-PCR (Qiagen) with a mixture of oligo dT and random primers ...
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bioRxiv - Biochemistry 2019Quote: ... Real time RT-PCR was performed in the RotorGene system (Qiagen) using SYBR Green ...
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bioRxiv - Microbiology 2020Quote: ... only modified by using the QuantiTect Probe RT-PCR Kit (Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... cDNA was synthesized and amplified using OneStep RT-PCR Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... which was performed using a QuantiTect Probe RT-PCR Kit (Qiagen) on the LightCycler 480 system (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... and analysed using a One Step RT-PCR kit (Qiagen, #210210) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Overlapping amplicons were generated using the OneStep RT-PCR kit (Qiagen) with 5 μl of cDNA as a template ...
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bioRxiv - Cell Biology 2023Quote: ... using the QuantiTect SYBR Green® RT-PCR kit (204243, Qiagen) as previously described [36] ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR for individual miR-17∼92 members was performed using miRCURY LNA probes and miRCURY LNA SYBR Green PCR Kit (Qiagen).
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bioRxiv - Bioengineering 2023Quote: The expression analysis of miR-21 was executed by using the miRCURY LNA SYBR Green PCR Kit (Catalog No. 339345, Qiagen) and primers from miRCURY LNA miRNA PCR Assays (Catalog No ...
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bioRxiv - Microbiology 2021Quote: Five nanograms of mRNA were analyzed by quantitative reverse transcription-PCR (qRT-PCR) using the QuantiFast SYBR green RT-PCR kit (Qiagen). qRT-PCR-based quantification of IL-6 ...
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bioRxiv - Molecular Biology 2021Quote: ... reverse transcription and quantitative real-time PCR (qRT-PCR) were performed with miScript II RT Kit and SYBR Green PCR Kit respectively (QIAGEN). To quantify α-Syn mRNA levels ...
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bioRxiv - Immunology 2023Quote: ... amplification of TCRβ-chain transcripts and library preparation was performed with the arm-PCR (ampliconrescued multiplex PCR) technology (iRepertoire Inc. Huntsville, USA) using the Qiagen OneStep RT-PCR Kit and Qiagen Multiplex PCR Kit (both Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Physiology 2023Quote: Quantitative real-time PCR (RT-qPCR) assays were performed using SYBR Green qRT-PCR master mix (QuantiNova SYBR Green PCR Kit, Qiagen) with primers designed using the Primer3 software (primer3.ut.ee ...
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bioRxiv - Immunology 2019Quote: ... plasma RNA samples were spiked with a standard of three exogenous single-stranded miRNA oligonucleotides (cel-miR-39, cel-miR-54, and cel-miR238; Qiagen) after the initial lysis step ...
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bioRxiv - Cell Biology 2021Quote: ... primary muscle progenitor cells were transfected with 100nM of miR-24-3p mimic (Syn-mmu-miR-24-3p, Qiagen, MSY0000219), 100nM of miR-24-3p inhibitor (Anti-mmu-miR-24-3p ...
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bioRxiv - Microbiology 2021Quote: For biotinylated RIPs HEK-293T cells were seeded and transfected with 20nM biotinylated LNA miR-29b or miR-30c (Qiagen) in combination with 2 μl Lipofectamine RNAi Max (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... miR-24-3p LNA inhibitor treatment: Cells were transfected with hsa-miR-24-3p miRCURY LNA miRNA Power Inhibitor (Qiagen, Germantown ...
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bioRxiv - Molecular Biology 2024Quote: ... and subsequently determined the expression of mature miR-146a and pre-miR-146a relative to SNORD68 using miRCURY SYBR green kit (Qiagen). The expression of TRAF6 was determined relative to GAPDH using iTaq Universal SYBR Green One step RT-qPCR kit (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2020Quote: Reverse transcription (RT) of miRNAs was carried out using miRCURY LNA Universal RT microRNA PCR Kit (Exiqon-Qiagen, Denmark), as instructed in miRCURY LNA RT Kit manual in three technical replicates for each miRNA ...
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bioRxiv - Microbiology 2020Quote: ... ARTD14 and ARTD15 were analyzed by quantitative real-time PCR (qRT-PCR) using QuantiTect Primer Assays (QIAGEN). In all settings the mRNA expression of the gene of interest was normalized to GUS (forward 5’-CTCATTTGGAATTTTGCCGATT-3’ and reverse 5’-CCGAGTGAAGATCCCCTTTTTA-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... Spike-in cel-miR-39-3p (MS00019789, Qiagen) was used as an internal control.
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bioRxiv - Cell Biology 2020Quote: ... All the miR mimics were purchased from Qiagen, Hilden ...
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bioRxiv - Molecular Biology 2023Quote: ... Mimics of mmu-miR-130b-3p (Qiagen, MSY0000387), mmu-miR-130b-5p (Qiagen ...
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bioRxiv - Evolutionary Biology 2019Quote: ... from the wild population by combining each forward primer with a reverse primer designed within the adjacent amplicon (Supplementary Table 6) and using the LongRange PCR kit (QIAGEN). When the primer sets originally designed from zebra finch sequences failed to produce long amplicons ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA generated after performing reverse transcription of RNA product was subjected to quantitative PCR (qPCR) analysis using gene-specific predesigned primers (RT2 qPCR Primer Assay, Qiagen), listed in Supplementary Table S4 ...
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bioRxiv - Neuroscience 2020Quote: ... Fold enrichment for each primer set was normalized to input and reported relative to a negative control gene desert region (mouse Igx1a (Qiagen). Primers ...
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bioRxiv - Immunology 2020Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cβ primer ...
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bioRxiv - Immunology 2024Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cý primer ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 nM of each primer and 5 µl of SYBR Green QuantiFast RT-qPCR master mix (Qiagen). Primers used were ...
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bioRxiv - Cancer Biology 2022Quote: ... primer array of the RT2 Profiler PCR Array (#PAMM-025ZD, Qiagen, Germany) was used according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... which used PyrG (CTP synthase) primers and HotStarTaq PCR reagents (QIAGEN, #203203). DNA samples were quantified then diluted to 20 ng/µL so that 5 µL could be used in each 25-μL PCR reaction ...
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bioRxiv - Bioengineering 2023Quote: ... and primers from miRCURY LNA miRNA PCR Assays (Catalog No. 339306, Qiagen) for hsa-miR-21-5p (GeneGlobe ID YP00204230) ...
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bioRxiv - Microbiology 2024Quote: ... Expression levels were measured by quantitative PCR using pre-designed primers (Qiagen) and SYBR green (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... and was subsequently subjected to reverse transcription followed by PCR using a OneStep RT-PCR Kit (QIAGEN) with primers for human Top2A ...
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bioRxiv - Microbiology 2021Quote: ... The assay was performed using qRT-PCR and the QuantiNova Probe RT-PCR kit (Qiagen, Düsseldorf, Germany), and the LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Genomics 2019Quote: ... The RT-PCR reactions were performed with a QuantiTect SYBR Green PCR Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: PCR amplification of the RNA was completed using the One-step Syber Green RT-PCR Kit (Qiagen). 25ng of total RNA was added to a master mix reaction of the provided RT Mix ...
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bioRxiv - Microbiology 2022Quote: PCR amplification of the RNA was completed using the One-step Syber Green RT-PCR kit (Qiagen). 25ng of total RNA was added to a master mix reaction of the provided RT mix ...
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bioRxiv - Microbiology 2024Quote: ... rpsL and qoxA transcripts by qRT‒PCR was performed with QuantiFast SYBR Green RT‒PCR Kit (Qiagen) using the Quantstudio3 system (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... BALF and serum was performed by quantitative reverse-transcriptase PCR (qRT-PCR) using the one-step Quantinova™ Probe RT-PCR kit (QIAGEN). Primers and a TaqMan probe were designed to hybridize to a sequence in ORF7 (encoding N) ...
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bioRxiv - Bioengineering 2021Quote: ... One-step reverse transcription quantitative polymerase chain reaction (RT-qPCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) with 7.8 μl purified viral RNA or specified quantities (2.5 ng or 100 ng ...
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bioRxiv - Cell Biology 2020Quote: ... was used to extract RNA and the OneStep RT-PCR Kit (Qiagen) was used to synthesize cDNA ...
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bioRxiv - Genomics 2020Quote: ... while the cDNA amplicons were made with OneStep RT-PCR kit (QIAGEN). Manufacturer protocols were followed for all experiments ...
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bioRxiv - Cell Biology 2019Quote: ... in a single step using the Qiagen OneStep RT-PCR Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Amplifications were done using the OneStep RT-PCR kit (QIAgen, California, USA) with the following conditions ...
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bioRxiv - Immunology 2021Quote: ... to perform RT² Profiler™ PCR Array Mouse Hypoxia Signaling Pathway (Qiagen) real-time PCR in Stratagene MX3000p qPCR system under these cycling conditions ...
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bioRxiv - Plant Biology 2020Quote: ... Amplification was carried out using a One-Step RT-PCR Kit (QIAGEN) and BIO-RAD T100 Thermal Cycler ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR experiments were performed with Rotor-Gene-Q machine (Qiagen) using the 1x Takyon SYBR Master Mix (Eurogentec ...