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Citations for Qiagen :
901 - 950 of 1003 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer A supplemented with 200 mM imidazole in gravity flow ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting with buffer A supplemented with 250 mM imidazole in gravity flow ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B supplemented with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... cleared by centrifugation (20 min, 20,000 × g, 4°C) and the clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer C supplemented with 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA agarose resin (QIAGEN) eluted with buffer A supplemented with 0.5 mM TCEP and 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and cleared lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysates were then incubated at 95°C for 5 min and filtered using a QIAshredder homogenizer (Qiagen, 79656). The extracted proteins were analyzed using 5–20% gradient sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... Tissue lysate was further used for DNA extraction with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany). Total DNA extraction of seawater filters was done using the entire filter ...
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bioRxiv - Immunology 2022Quote: Total RNA from CD1c+ DC cell lysates from patients and controls was isolated using the AllPrep Universal Kit (Qiagen) on the QIAcube (Qiagen ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were centrifuged for 30 min at 45,000 × g and incubated with 1 ml of Ni-NTA agarose (Qiagen) per 1 l expression culture ...
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bioRxiv - Immunology 2019Quote: ... Tissues were homogenized and lysate supernatants were subjected to RNA isolation using the RNeasy-Plus kit (Qiagen, Valencia, CA).
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bioRxiv - Genomics 2021Quote: Tissue lysates were prepared from 30 pooled 2 dpf WT and MZdnmt3aa-/- mutant by homogenization using TissueLyser LT (QIAGEN) with zirconia beads for 10 s at 50 Hz ...
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bioRxiv - Biochemistry 2021Quote: ... One half of clarified cell lysate (2 ml) was applied to 200 μl of Ni-NTA Superflow resin (Qiagen) equilibrated with Ni wash buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from EGFP+ FAC-sorted and unsorted cell lysates by using the RNeasy mini kit (Qiagen) according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were equalized and His6-HA-SUMO1-conjugates were enriched on nickel-nitrilotriacetic acid (NiNTA) agarose beads (Qiagen, #L30210) as described in15 ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from right hemisphere brain lysates using a QIAGEN RNeasy kit (Catalogue #74104, QIAGEN, Hilden, Germany) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Lysates for RNA purification were prepared by aspirating growth medium and triturating hESC-RPE cultures in RLT Buffer (Qiagen) followed by storage at -80°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysates were further transferred to liquid N2/ -80 °C freezer before RNA purification according to Qiagen protocol (www.Qiagen.com ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified bacterial lysates from a 1 l culture were bound to a 0.5 ml column of Ni-NTA resin (Qiagen) by gravity flow ...
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bioRxiv - Neuroscience 2023Quote: Total RNA of MSN cultures over time was extracted and purified from cell lysates using RNeasy Mini kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: RNA was extracted from cell lysates using the RNeasy Mini Kit and subjected to a DNase treatment according to manufacturer’s protocols (Qiagen) then stored at −80 °C until further use ...
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bioRxiv - Microbiology 2023Quote: ... Lysate was centrifuged (6000rcf, 5 minutes) and genomic DNA (gDNA) extracted from pellets also using the DNeasy kit (QIAGEN). Successful gene modification events or maintenance of the wildtype loci was performed by PCR using primers listed in Table S5.
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bioRxiv - Biochemistry 2023Quote: ... Soluble lysate was prepared by centrifugation and the kinase domain of FGFR1 was isolated by Ni-NTA column (Qiagen).
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from these cell lysates using the RNeasy UCP Micro Kit protocol and reagents (Qiagen, Cat #73934). DNA was digested via on-column DNase I treatment for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... and 9-10 mL of Buffer BB (1/3 of the lysate volume, From QIAGEN Plasmid Plus Midi Kit) was added ...
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bioRxiv - Microbiology 2020Quote: ... and then cell lysates were collected by washing cells twice with PBS and adding 100ul of RNA lysis buffer (Qiagen) to each well.
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bioRxiv - Cell Biology 2020Quote: ... DNA was removed by passing the lysate through the gDNA eliminator column provided with the kit and by on-column DNase treatment before elution (Qiagen). RNA was eluted using nuclease-free water (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... Clarified lysate from bacterial expression was passed over a column with Streptactin Superflow resin or Streptactin XT Superflow resin (Qiagen). After incubation ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and dARC1 CA captured from clarified lysate using immobilised metal ion affinity on a 5 mL Ni2+-NTA superflow column (Qiagen). Bound dARC1 CA was eluted in non-reducing buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... at 60°C overnight and 1 μl of a 1:20 dilution of the lysate used in a PCR reaction (HotStarTaq, Qiagen). The sequence flanking the tmt-opsin1b TALEN binding sites was amplified using the forward 5’-GGGACTTTCTTTGCGCTTTA-3’ and the reverse 5’-CAGGTCAGAGCGGATCTCAT-3’ primers ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was cleared by centrifugation and an aliquot taken for whole lung RNA preparation using the RNeasy Mini kit (Qiagen). The remaining lysate was incubated for 4 hours with a 1:20 dilution of anti-HA magnetic beads (Pierce 88837) ...
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bioRxiv - Neuroscience 2020Quote: RNA was purified from the lysate following the manufacturer’s protocol using a combined protocol of QIAzol® (Qiagen, Hilden, Germany) and NucleoSpin® (Macherey-Nagel ...
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bioRxiv - Cancer Biology 2021Quote: RNA from infected cell lysates was harvested by incubating infected Vero E6 cells for 10 minutes with RLT buffer (Qiagen) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... the cleared cell lysate was loaded onto a gravity column with 0.5 mL bed volume of Ni-NTA resin (Qiagen, #30210). The resin was extensively washed ...
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bioRxiv - Cell Biology 2022Quote: ... The cleared lysate was then incubated with 3 mL of 1:1 Ni-NTA agarose slurry (Qiagen, product number 30210) for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was removed by passing the lysate through the gDNA eliminator column and by an additional on-column DNase I treatment (Qiagen) before the elution ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl clarified lysate were mixed with 70 μl RNase free water and RNA isolated using the RNeasy Mini Kit (Qiagen) according the manufacturers recommendations ...
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bioRxiv - Microbiology 2021Quote: ... HBV DNA was extracted from cell lysates per manufacturer’s instructions (Qiagen DNeasy 96 Blood and Tissue Kit, Qiagen, Hilden, Germany). HBV DNA was detected by qPCR using primers and probe as follows ...
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bioRxiv - Biochemistry 2020Quote: A cell pellet corresponding to 20-25 OD600 units was lysed with glass beads and RNA was isolated from the cell lysate using the RNeasy Mini Kit and RNase-free DNase Set (both Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Total RNA was extracted from sputum cell pellets or bronchial epithelial cell lysates stored in RLT buffer (RNeasy kit, Qiagen) and 2μg was used for cDNA synthesis (Omniscript RT kit) ...
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bioRxiv - Biochemistry 2019Quote: ... and the lysate was mixed gently with 4 ml (50% slurry) of nickel-nitrilotriacetic acid (Ni-NTA)-agarose resin (Qiagen) at 4°C for 1 h ...
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bioRxiv - Genomics 2021Quote: ... Nucleic acids were purified from the lysates using the Qiagen AllPrep DNA/RNA mini kit (Qiagen Inc., Valencia, CA, USA), quantified using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Purification of p53-(1-73) from the clarified cell lysate was accomplished using a Nickel Nitrilotriacetic Acid (Ni-NTA, Qiagen) column purification and 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris was removed by centrifugation at 20000 g for 40 min at 4 °C and the lysate incubated with Ni-NTA Agarose Beads (Qiagen). After washing ...
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bioRxiv - Cancer Biology 2020Quote: ... we captured poly-adenylated mRNA from cell lysates using a 96-well plate with oligo(dT) grafted to the inner walls of each well (Qiagen). Next ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA was extracted from cell lysates using the RNase Mini Kit where genomic DNA was removed using the RNase-Free DNase Kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were passed five times through a 23G needle before proceeding to RNA extraction using RNeasy Qiagen Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysate was centrifuged at 10,000g for 45 minutes and the supernatant was loaded onto Ni-NTA column (Qiagen, Germany). The unbound protein was washed with washing buffer (50 mM Tris buffer pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Full-length his-tagged ySgt2 and cysteine mutants were separated from the lysate by batch incubation with Ni-NTA resin (Qiagen) at 4°C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatant of the bacteria lysate was transferred into new 50 ml Falcon tubes and incubated with Ni-NTA (Qiagen) at 4 °C for 30 min ...