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Citations for Qiagen :
651 - 700 of 948 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Altered motility in response to iron-limitation is regulated by lpdA in uropathogenic E. coli CFT073bioRxiv - Microbiology 2023Quote: ... Strains were cultured for five hours at 37°C with aeration before harvest and treatment with bacterial RNAprotect (Qiagen). Bacterial pellets were stored at -80°C until RNA isolation.
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bioRxiv - Microbiology 2024Quote: ... was used to amplify DNA fragment except for PCR verification of plasmids or strains for which Taq polymerase (Qiagen) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting plasmid was then transformed into E.coli DH10b strain for isolation via QIAprep Plasmid Mini-Prep Kit (Qiagen). The isolated plasmid was subsequently transformed into D.radiodurans R1 chemically competent cells (above) ...
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bioRxiv - Genomics 2020Quote: ... The supernatant of cell lysate was incubated with 1 mL Ni-NTA agarose beads (QIAGEN) at 4 °C for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... and the lysates were processed through the DNeasy blood and tissue kit (Qiagen, Valencia, CA), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Liver lysates were made by homogenization following the manufacturer’s instructions using a Tissue Lyser (Qiagen). Hepatic glycogen levels were normalized for protein concentrations determined by Bradford Reagent (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... The lysates from the EmulsiFlex B-15 homogenizer were added to Ni-NTA agarose (Qiagen) and incubated on the nutator at 4 °C for 1 h ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was extracted from cell lysates using RNeasy Mini Kit (Qiagen, Valencia, CA, USA), according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... The soluble cell lysate fraction was loaded on a 4 mL Ni-NTA column (QIAGEN) pre-equilibrated with binding buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was clarified by centrifugation and bound to Ni-NTA agarose beads (QIAGEN #30230) for 45 min at 4ºC ...
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bioRxiv - Bioengineering 2022Quote: ... Lysates were eluted with RNase-free water after purification using a RNeasy Mini Kit (Qiagen) and DNase I digestion ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA in tissue lysates was extracted using the QIAamp Viral RNA Mini Kit (Qiagen) and subjected to qRT-PCR assays ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was then incubated for one hour at 4 °C with Ni-NTA (Qiagen) in batch adsorption format ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 48k rcf and bound to Ni-NTA agarose beads (Qiagen). The beads were washed with the resuspension buffer and eluted with 20 mM Tris HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified from clarified lysate by affinity chromatography on Ni2+-nitrilotriaceta-agarose (Qiagen) in 20 mM Tris-HCl 500 mM NaCl pH 8.0 buffer and by size exclusion chromatography on Superdex 200 HL 16/600 column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... BapR-CPD-His was affinity purified from cleared lysates using Ni-NTA agarose beads (Qiagen) with gentle rocking at 4°C for 2 hours ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysate (20 mL) was incubated with 10 mL of Ni-NTA agarose (QIAGEN, 30230) for 30 min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Lysate was spun down and supernatant was mixed with 2 ml Ni-NTA resin (Qiagen) per 50 ml sample and incubated for 1h at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... All the proteins were obtained in cell lysates and subsequently purified using Ni-NTA (Qiagen) affinity-columns and superdex 200 increase 10/300 GL (GE Lifesciences ...
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bioRxiv - Microbiology 2021Quote: ... the clarified lysate was bound using the batch method to Ni-NTA agarose resin (Qiagen) at 4°C for 1 hour ...
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bioRxiv - Genomics 2019Quote: ... The cell lysate was sampled (100 μL) and mixed with 700 μL of QIAzol (QIAGEN) for total RNA extraction ...
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bioRxiv - Biochemistry 2020Quote: ... lysate was clarified by centrifugation and the supernatant was incubated on Ni-NTA resin (Qiagen). The resin was washed with wash buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... coli lysates under native conditions by immobilised metal affinity chromatography using Ni-NTA agarose (Qiagen) essentially as described 12 using 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the soluble fraction of the lysate was loaded onto a Ni-NTA Superflow Cartridge (QIAGEN) pre-equilibrated in wash buffer (50 mM HEPES-Na ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was then batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin at 4°C for 1 hour in a beaker set on a stir plate ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysates were mixed with 0.5 ml Ni-NTA beads (250 μl bed volume, Qiagen), pre-washed with two times 5 ml lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... The clarified cell lysate was loaded onto a 5 mL Ni-NTA superflow cartridge (Qiagen), washed with buffer A ...
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bioRxiv - Immunology 2021Quote: RNA extraction was performed on the lysate using the RNeasy Mini Kit (Qiagen, Hilden, Germany). Libraries were prepared as previously described.22 Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The filtered lysate was then loaded onto a 5 ml Ni-NTA Superflow column (Qiagen), and the column was washed with buffer A containing 10 mM imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was immediately isolated from the lysate using the RNeasy kit (Qiagen, Germantown, MD), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... lysate samples were thawed at room temperature and then processed using QIAshredder (Qiagen, Cat# 79654) and Rneasy Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was purified by affinity chromatography on a Ni-NTA Superflow Cartridge (Qiagen) using an ÄKTA Start (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was purified by affinity chromatography on a Ni-NTA Superflow Cartridge (Qiagen) using an ÄKTA Start (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cleared lysate was purified by affinity chromatography on a Ni-NTA Superflow Cartridge (Qiagen) using an ÄKTA Start (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... coli lysates under native conditions as described in the fifth edition of the QIAexpressionist (Qiagen). The lysates were loaded onto a 1 ml HisTrap HP column (GE healthcare ...
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bioRxiv - Microbiology 2023Quote: ... was further added to the lysate prior to using a bead beater (TissueLyser II, Qiagen) for homogenizing the mixture for 5 min at a speed of 30 beats/sec ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant lysates were purified on Ni-NTA agarose beads (for His6-tagged proteins, Qiagen) or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was then batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin at 4ºC for 2 hours in a beaker set on a stir plate ...
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bioRxiv - Genomics 2023Quote: ... RNA from the lysate was extracted and purified with an RNeasy Mini Kit (QIAGEN #74104) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The cleared lysate was loaded onto two 5 mL Ni-NTA Superflow cartridges (QIAGEN, 30761) equilibrated with lysis buffer and washed with 10 column volumes of washing buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate was passed over a gravity column of 8 mL of Ni-NTA resin (Qiagen), washed with 70 mL wash buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was centrifuged and the soluble cell fraction was incubated with Ni2+ –NTA (Qiagen) beads for 3 hr ...
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bioRxiv - Microbiology 2023Quote: ... the lysate was processed using the described protocol of the RNeasy Mini kit (Qiagen, Germany). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... The clear lysate was transferred to a QIAilter Catridge (From QIAGEN Plasmid Plus Midi Kit) and incubated at room temperature for 10 mins ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was then incubated with 5 mL of Ni-NTA agarose beads (Qiagen), pre-equilibrated in lysis buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from the lysate using the DNeasy® Plant Mini Kit (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted from RLT Plus lysates using the RNeasy Plus Mini Kit (QIAGEN, #74136), including on-column DNase treatment using the RNase-Free DNase set (QIAGEN ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was briefly incubated (approximately 5 min) with 3 ml Ni-NTA Agarose (QIAGEN) before flow-through was collected ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... 6xHis tagged proteins were purified from the clarified lysates by using Ni-NTA beads (Qiagen), and incubated with end-over-end rotation overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Viral nucleic acids were then isolated using the Qiagen QIAamp MinElute Virus Spin Kit without the use of AW1 buffer or carrier RNA (Qiagen, Valencia, CA, USA). Random hexamers were used to prime cDNA synthesis (Life Technologies ...