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Citations for Qiagen :
701 - 750 of 948 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in 384 well plates on an Applied Biosystems 7900HT Fast Real-Time PCR system using the QuantiTect Virus Kit (Qiagen, Redwood City, CA) and SARS-CoV-CDC RUO primers and probes (Integrated DNA Technologies (IDT) ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acids were extracted at CNM using either QIAamp MinElute Virus Spin (DNA) or QIAamp Viral RNA Mini kits (Qiagen, Germantown, MD, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA was extracted for the ancestral strain (AN) and a single clonal isolate from each evolved population (S1-S10) using QIAamp DNA Blood Midi Kit (Qiagen) following the manufacturer’s recommendations from 50 mL culture incubated at 17 °C for 5 days in 75 cm2 flasks ...
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bioRxiv - Genomics 2022Quote: ... The genomic DNA of the wild-type and the mutant strains was isolated using a QIAamp DNA Mini Kit (QIAGEN). Libraries were prepared using the Nextera XT DNA Library preparation kit (Illumina) ...
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bioRxiv - Molecular Biology 2020Quote: ... coli strain (Rosetta) and purified using Ni-NTA affinity chromatography according to the manufacturer’s instructions (Qiagen Cat No./ID: 30210). Purified protein was used as an immunogen to raise polyclonal antibody in rabbits at a local commercial facility (DPL ...
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bioRxiv - Microbiology 2019Quote: ... The complete 16S rRNA gene was amplified in specific strains after DNA extraction using the DNeasy Blood & Tissue kit (Qiagen), following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2019Quote: Genomic DNA from each strain was isolated from overnight cultures using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... genomic DNA was prepared from 20 adult female flies from each strain using the DNeasy Blood and Tissue kit (Qiagen). PCR was performed with primers specific to the LcαE7 gene16 ...
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bioRxiv - Microbiology 2021Quote: ... 500 μL of mid-log phase (OD600nm = 0.45 - 0.7, strain-specific) cells were stabilized using RNA Protect Bacteria Reagent (QIAGEN, Hilden, Germany), and cell pellets stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... and RNA was isolated from 3 biological replicates of each strain when the cultures had reached OD600 = 0.1 using the RNeasy mini kit (Qiagen #74106). RNA-Seq was performed at the Genomics Core Facility at Princeton University ...
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bioRxiv - Cell Biology 2020Quote: RNA was isolated from frozen RNA later® soaked tissue from the embryo from either C57BL/6NCrl or Ddi2PD strains using RNeasy plus Micro and Mini Kits (QIAGEN). Samples with RIN values above 7 in the Bioanalyzer RNA 6000 Nano assay (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant protein fragments were produced in Escherichia coli (strain M15) as fusion products with an N-terminal tag of six histidines using plasmid pQE30 (Qiagen) and purified from total bacterial lysates by nickel affinity chromatography ...
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bioRxiv - Microbiology 2022Quote: ... Transformation of gene knockouts from strains 168 or PY79 into strain 3610 was done with the following protocol: DNA was extracted from strain 168 or PY79 with the DNeasy Blood & Tissue kit (Qiagen) as described in the manufacturer’s protocol and the final DNA yield was quantified by Qubit (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... all clinical isolates and the UCBPP-PA14 reference strain were cultivated at 37 °C in LB broth and harvested in RNAprotect (Qiagen) at OD600= 2 ...
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bioRxiv - Epidemiology 2019Quote: The genomic DNA of the 11 outbreak strains was extracted using the DNeasy blood and tissue kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from cultured tprAko-SS14 or wild-type strains propagated in 6-well plates following the transformation procedure was performed using the QIAamp mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Most DNA samples for ONT sequencing were extracted from 100 female flies from each strain using the Blood and Cell Culture DNA Mini Kit (Qiagen) following manufacturer’s instructions with small modifications (Table S2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we designed strain-specific primers (see Supplementary Table 2) and generated overlapping amplicons using the OneStep RT-PCR Kit (Qiagen) and 5 µL of cDNA ...
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bioRxiv - Biochemistry 2021Quote: Genomic DNA was isolated from 2 clones of each strain corresponding to 50 OD600 units with the Genomic DNA Buffer Set and Genomic-tip 100/G (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The gDNA of all mutants strains constructed in this study was extracted using DNeasy® Blood and Tissue kit (Qiagen) according to the manufacturer recommendations ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA from 110 mutants plus the ancestral strain was extracted with the Genomic-tip 20/G Kit (Cat. No. 10223, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... coli HST04 dam-/dcm- strains in which target MTases were transformed were extracted using the DNeasy UltraClean Microbial Kit (QIAGEN) according to the supplier’s protocol after induction of gene expression ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from MU636 and set1 mutant strains growing in plates using the RNeasy Plant Mini Kit (Qiagen) and treated with DNase (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... We extracted gDNA from the MSK named strains collected in Botswana and North Carolina using the Powersoil Pro extraction kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... coli strain M15-[pREP4] (for transformation of recombinant plasmids) and RNAse (for protein purification) were purchased from Qiagen (Valencia, CA). All other chemicals were obtained from Sigma-Aldrich (St ...
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bioRxiv - Molecular Biology 2024Quote: ... coli XL1-blue strain was transformed and plasmids were isolated from bacterial cultures using the Plasmid Midi Kit (Qiagen, Germany). Sequencing of the three NS1 plasmids was performed by Eurofins (Germany).
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA from mutant clones with enhanced tdTomato-ATG8 signal compared to the parental strain was harvested using DNeasy Blood & Tissue Kit (Qiagen) and send for sequencing at UMICH Advanced Genomics Core ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were then processed for RNA and DNA extraction using AllPrep DNA/RNA Mini kit (Qiagen), with Complete Protease Inhibitor (Roche ...
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bioRxiv - Molecular Biology 2020Quote: RNA extractions from frozen lysates were performed on the same day using RNeasy Mini columns (Qiagen). Recovered total RNA was quantified by Qubit and quality was assessed using an Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate was cleared by centrifugation and the soluble fraction was applied to Ni-NTA resin (Qiagen) and incubated for 1.5 h ...
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bioRxiv - Microbiology 2019Quote: ... RNA was then isolated from cleared lysate using the manufacture’s protocol (#74106 RNeasy Mini Kit Qiagen).
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bioRxiv - Genetics 2020Quote: DNA/RNA extraction was performed from cell lysates using the AllPrep DNA/RNA Micro Kit (Qiagen). For initial optimisation experiments using an HPRT gRNA-containing RNP (or non-targeting control) ...
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bioRxiv - Genetics 2020Quote: ... Total DNA was isolated from the cell lysates using the QIAquick® PCR Purification Kit (QIAGEN). The following primers used were designed to the lucia DNA sequence for amplification of the reporter gene (JE041 ...
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bioRxiv - Microbiology 2021Quote: ... Toxins were then purified from bacterial cell lysates through affinity chromatography on a Nickel-resin (Qiagen), ion exchange ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate from bacterial expression was passed over a column with Streptactin XT Superflow resin (Qiagen). After incubation ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation and the supernatant was applied to Ni NTA-agarose (Qiagen), which was washed with the same buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate fraction was bound in batch to nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The resin was washed extensively with 20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate fraction was bound in batch to nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The resin was washed extensively with 20 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was bound to a 2 mL slurry of Strep-Tactin Superflow plus (Qiagen) overnight at 4°C using the batch method ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acids were isolated from cell lysates or lung homogenates using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The clarified lysate was then loaded onto an open column containing pre-equilibrated Ni-NTA (Qiagen). The column was washed with 20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysate was then transferred to the RNeasy spin column provided in the RNeasy kit (Qiagen). The manufacturers protocol was then followed for the rest of the isolation process.
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bioRxiv - Cell Biology 2020Quote: ... The lysate was centrifuged and the supernatant was load onto a Ni-NTA affinity column (Qiagen). Proteins were eluted with elution buffer (20 mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was first purified on a Ni–nitrilotriacetic acid (NTA) column (QIAGEN, Valencia, CA). The eluate was further purified on glutathione–Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was purified over Ni-NTA affinity resin according to the manufacturer’s recommendation (Qiagen). Eluted proteins were pooled and dialyzed overnight at 4°C into 50 mM Na2HPO4 pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... The bacterial lysates were mixed for 1 h with 5 ml of Ni-NTA resin (Qiagen) that had been equilibrated with buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysates were spun on the QIashredder column and RNA extracted using the RNeasy kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... and the supernatant of the bacterial lysate was loaded to a disposable column (Qiagen, Hilden, German) pre-packed with glutathione agarose (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified lysate was applied to two tandemly-connected 5 ml Ni-NTA Superflow cartridges (Qiagen); the cartridges were washed with buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... mouse organ lysates and human primary cell supernatant samples were prepared in RNeasy Mini Kit (Qiagen) lysis buffer RLT (400μl) ...