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Citations for Qiagen :
551 - 600 of 948 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Genomic DNA of the selected clone of both strains was isolated using QIAamp DNA blood mini kit (Qiagen) for whole genome sequencing using Illumina HiSeq 150 PE ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA of clinical strains was prepared from overnight cultures using the DNeasy blood and tissue kit (Qiagen). Illumina whole genome sequencing was performed by the Plateforme de microbiologie mutualisée (P2M ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA of the RCA strains was extracted using a DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... RNA samples from 3 independent cultures for each strain (Chr_dam and Chr_gfp) were extracted with RNeasy miniprep kit (Qiagen). Primers used are listed in Table S1 ...
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bioRxiv - Genetics 2019Quote: ... Reads were mapped against the reference genome of strain S288C and variants detected using CLC Genomics Workbench (Qiagen).
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bioRxiv - Microbiology 2023Quote: Genomic DNA was isolated from the clinical strains obtained using DNeasy Blood and tissue kit (Qiagen cat. 69504). DNA was sent to Microbial Genome Sequencing Center ...
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bioRxiv - Genomics 2023Quote: ... or the buffy coat of the BALB/c strain using the QIAsymphony DNA Mini Kit (cat:931236, Qiagen). The extracted gDNA was sheared to 150 bp in size using a Covaris LE 220 instrument ...
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bioRxiv - Microbiology 2024Quote: ... and mapping of the reads on a reference (GenBank strain: U39292) using CLC genomics workbench software v.21.0.5 (Qiagen). Parameters for reference-based assembly consisted of match score = 1 ...
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bioRxiv - Microbiology 2021Quote: ... To generate a recombinant A_NS237 virus, viral RNA was extracted from allantoic fluid using Trizol reagent (Thermo Fischer, Germany) and Qiagen RNeasy Kit (Qiagen, Germany) following the manufacturers’ guidelines ...
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bioRxiv - Genomics 2020Quote: ... Viral RNA was extracted from 300 µl of viral transport media (VTM, Himedia, India) using QIAamp 96 Virus QIAcube HT Kit (Qiagen, Germany), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: A 200 μL aliquot of Copan UTM® was processed through the QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen, Cat# 937036) following manufacturer’s instructions on the QIAsymphony SP instrument ...
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bioRxiv - Molecular Biology 2020Quote: The RNA isolation was performed with 50 µl of the crude extract on the QIAsymphony with the DSP Virus/Pathogen Kit (Qiagen, #937055). The RT-PCR was performed using 5 µl of 85 µl eluate with TIB MolBiol Lightmix® MODULAR SARS AND WUHAN CoV E-Gene Kit ...
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bioRxiv - Genomics 2020Quote: Viral RNA was extracted from nasopharyngeal swabs of patients with COVID-19 using the EZ1 DSP Virus Kit (Qiagen, Hilden, Germany), optimized for viral and bacterial nucleic acids extractions from human specimens using magnetic bead technology ...
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bioRxiv - Microbiology 2021Quote: ... we collected the cell culture supernatants after 24 h of infection for viral RNA extraction with a QIAamp 96 Virus QIAcube HT Kit (Qiagen, 57731) and for viral RNA copy number detection in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from the lungs of influenza virus-infected mice using the RNeasy Mini Kit (QIAGEN, Cat. No 74004) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs from clarified lysates (60 μl) were isolated using the miRNeasy mini kit (Qiagen) according to manufacturer’s standard protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... genomic DNA was isolated from tissue lysate with DNeasy Blood & Tissue kit (Qiagen, 69504). mtDNA copy number was determined by quantitative PCR with Human mitochondrial to nuclear DNA ratio kit (Takara Bio USA ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was isolated from tissue lysates using the RNeasy Mini Kit (cat# 74104, Qiagen).
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared and bound to pre-equilibrated Ni-NTA agarose beads (Qiagen) for 1.5 h ...
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bioRxiv - Genomics 2020Quote: ... gDNA was then purified from the lysate using the QIAamp DNA mini kit (Qiagen) according manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The lysate was used for DNA isolation with Qiagen DNeasy Plant Mini Kit (Qiagen) following the manufacturer protocol ...
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bioRxiv - Cell Biology 2019Quote: ... The cleared lysate was loaded onto a pre-equilibrated Ni-NTA agarose column (Qiagen) and washed with buffer A supplemented with 30 mM imidazole ...
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bioRxiv - Cancer Biology 2020Quote: ... genomic DNA was isolated from cell lysates with a QIAamp DNA Mini Kit (Qiagen). Barcode sequences were amplified by PCR using 300 ng of genomic DNA (100,000-genome and 500,000-barcode equivalent ...
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bioRxiv - Biochemistry 2019Quote: ... The clarified lysate was first purified on a Ni-NTA column (Qiagen, Valencia, CA). The eluate was further purified on glutathione-Sepharose 4B resin (GE healthcare ...
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bioRxiv - Immunology 2021Quote: ... then clarified lysates were loaded onto a Ni2+ affinity column (Ni-NTA Superflow; Qiagen), washed in buffer A plus 300 mM NaCl and 20 mM imidazole pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... then clarified lysates were loaded onto a Ni2+ affinity column (Ni-NTA Superflow; Qiagen), washed in buffer A plus 300 mM NaCl and 20 mM imidazole pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were passed over 5 ml of packed Ni-NTA agarose resin (Qiagen) at 1.0 ml/min ...
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bioRxiv - Cell Biology 2021Quote: ... The cleared lysate was run through Ni+2-NTA agarose beads (Qiagen [QA], 30250) to allow binding of the histidine-tagged (recombinant ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysate was added to a column with Ni-NTA agarose (QIAGEN, Hilden, Germany) and then washed with cold lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... and the clarified lysate was incubated in batch with Ni-NTA agarose resin (Qiagen) for 1 hour at 4°C with rotation ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysate was added to 1 mL of washed Ni-NTA bead slurry (Qiagen) and incubated for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were subjected to DNA extraction using the AllPrep DNA/RNA Mini Kit (Qiagen) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... and RNA was extracted from the lysates using an RNeasy® Mini kit (Qiagen). Purified DNA-free RNA was quantified ...
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bioRxiv - Microbiology 2020Quote: ... Lysate was applied to a prepacked gravity column of Ni-NTA agarose beads (Qiagen) equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... The gDNA was purified from the lysate using a QIAamp DNA mini kit (Qiagen). Sequence libraries of the V1 and V3 region of the 16S rRNA genes from each of the samples were constructed in accordance with the Dual Barcoded Two-Step PCR procedure from Illumina (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was loaded on 100 µl of Ni-NTA agarose beads (QIAGEN), pre-washed twice with 1 ml of denaturing washing buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was purified from cleared lysate using a QIAamp DNA mini Kit (Qiagen). For targeted NGS ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysate supernatant was passed through a Ni+2-NTA resin column (Qiagen), and protein was purified following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was gently shaken with 0.5 ml of Ni-NTA agarose suspension (Qiagen) for 1 h at 4 °C and transferred to a filter column where the liquid was drained ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Uncleaved protein with 6xHisTag were isolated from the lysate using Ni-NTA resin (Qiagen). The flow-through (FT ...
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bioRxiv - Cell Biology 2022Quote: ... Ear skin lysates were prepared by homogenizing the tissue with Homogenizer TissueRuptor II (QIAGEN) in TBS + 1% Triton-X100 + Protease and Phosphatase Inhibitor Cocktail (Sigma) ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant of the cell lysate was loaded onto Ni-NTA agarose resin (Qiagen) in Poly-prep chromatography columns (BioRad) ...
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bioRxiv - Genomics 2020Quote: ... Total RNA was then extracted from cell lysates using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNAs were extracted from homogenized cell lysate using RNEasy Plus mini kit (Qiagen), and the quality of extracted total RNA were examined using Nanodrop spectrum analysis and agarose gel electrophoresis ...
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bioRxiv - Developmental Biology 2019Quote: ... Frozen tissues were crushed with a pestle and the tissue lysates homogenized (QIAshredder, QIAGEN). Total RNA was isolated (RNeasy Mini Kit ...
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bioRxiv - Immunology 2020Quote: ... Residual DNA was removed from the cleared lysate using DNase digestion on columns (Qiagen). mRNAs were eluted in 40μL of RNase-Free water ...
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bioRxiv - Genetics 2020Quote: ... Cell lysates were incubated with 100 μl of Ni-NTA superflow beads (Qiagen, 30430) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was extracted from total cell lysate and cytosolic fractions using DNeasy kit (Qiagen). DNA samples were each diluted 1:10 and corresponding primers (Supplementary Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... was added to each dish and the lysate was collected into Qiashredder tubes (Qiagen) and centrifuged at 12,000 x g for 30 seconds for homogenization ...
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bioRxiv - Immunology 2021Quote: ... The clear solution containing lysate was passed through the RNeasy mini column (Qiagen, Germany), which leads to the binding of RNA with the column ...