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Citations for Qiagen :
901 - 950 of 978 citations for Scyliorhinin II amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... and tissue pellets were homogenized for 2.5 min at 25 Hz in a mixer mill (Tissue Lyser II, Qiagen, Hilden, Germany). Following this ...
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bioRxiv - Pathology 2021Quote: ... Samples were lysed in 175 μL of RNA lysis buffer added with β-mercaptoethanol and 350 μL of RNA dilution buffer for tissue disruption using a TissueLyser II LTTM (Qiagen). Samples were incubated at 70°C for 3 min ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and DNA was extracted using a TissueLyser II bead mill together with the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following supplier recommendations.
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bioRxiv - Biochemistry 2019Quote: ... genomic DNA was de-crosslinked in ChIP elution buffer containing 5 M NaCl at 65 °C overnight and purified with the Qiaex II kit (Qiagen, 20021) and eluted in water for PCR amplification ...
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bioRxiv - Microbiology 2019Quote: ... Tissue samples were homogenized with the kit Lysis buffer and 2.8 mm ceramic beads (MO BIO Laboratories Inc., Carlsbad, USA) using TissueLyser II (Qiagen, Hilden, Germany). Subsequently total RNA was extracted as described in the protocol with a DNase I treatment step as per the manufacturer’s instructions ...
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Revisiting a GWAS peak in Arabidopsis thaliana reveals possible confounding by genetic heterogeneitybioRxiv - Genetics 2021Quote: ... Tissue was disrupted by adding two small stainless beads bearings and agitating with a tissue lyser (tissuelyser II, Qiagen, Hilden, Germany) for 10 min at 20 rev/s ...
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bioRxiv - Microbiology 2022Quote: ... PCR products of approximately 440 bp were visualised by gel electrophoresis and gel-purified using the QIAEX II Gel Extraction Kit® (QIAGEN). The purified barcoded amplicons were quantified by Qubit™ dsDNA BR Assay Kit (Life Technologies ...
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bioRxiv - Pathology 2022Quote: ... tissue samples were homogenized in 1 ml of phosphate buffered saline with a metal bead using a TissueLyzer II (Qiagen GmbH). Viral nucleic acids were extracted from blood and homogenized spleen ...
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bioRxiv - Molecular Biology 2023Quote: Isolated RNA samples were reverse-transcribed into cDNA in 20 μl final reaction volumes using miScript II RT Kit(Qiagen,,Germany) as specified in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Complementary DNA (cDNA) was subsequently generated using 2 μg of total RNA and the miScript II Reverse Transcription (RT) Kit (Qiagen, Australia), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 500ng of RNA were used to perform reverse transcription using the miScript RT II Kit and HiFlex Buffer Reverse Transcription (Qiagen, Netherlands). Quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Physiology 2023Quote: ... 1 µg of RNA isolated from human islets or 10 µL of exosomal RNA was reverse transcribed using the miRScript II kit (Qiagen, Germany), following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Sand and rhizosphere samples were prepared by adding 250 mg of the collected substrate to a PowerBead plate and homogenized using a TissueLyser II (Qiagen, USA) at a frequency of 20 Hz for 10 minutes ...
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bioRxiv - Genomics 2023Quote: Flash frozen male pupa was homogenized in 650µl of homogenization buffer (10mM Tris-HCL-pH 8.0 and 25mM EDTA) using TissueRuptor II (Qiagen, Germany; Cat # 9002755). 650µl of lysis buffer (10mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... and miRNAs from each HTM cells was reverse transcribed into cDNA using miScript II RT kit as per the manufacturer’s instruction (Qiagen, Hilden, Germany). RT-qPCR was performed in a total volume of 20 µl containing 25 ng of total cDNA ...
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bioRxiv - Physiology 2023Quote: Whole adipose tissue or isolated primary adipocytes and aorta were mechanically homogenised with 5mm stainless steel beads using a TissueLyser II system (Qiagen, UK) in the presence of 700μL QIAzol Lysis Reagent (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... leaf-discs were collected and ground in autoclaved Milli-Q water using a TissueLyser II (QIAGEN; 45 seconds at 30 Hz). Serial dilutions of the ground tissue were spotted onto low salt Luria Bertani (LB ...
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bioRxiv - Genetics 2023Quote: ... Further confirmation of which allele was deleted in clones with only one UBE3A copy was performed by utilizing the EpiTect Methyl II DNA Restriction Kit (QIAGEN, #335452) to measure methylation at the PWS-IC (SNRPN ...
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bioRxiv - Microbiology 2023Quote: ... one aliquot was not processed any further and one other aliquot was bead beaten with 100 mg of sterile glass beads (400-600 nm) for 30 s at 30 Hz in a Tissue-lyser II (Qiagen, Germany), in order to open up Symbiodiniaceae cells and release intracellular bacteria to facilitate their growth on agar plates ...
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bioRxiv - Immunology 2023Quote: ... 120 mg l− 1 sodium pyruvate and 1% penicillin–streptomycin) at 300 Hz for 2 minutes using a TissueLyser II (Qiagen, Germany).
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bioRxiv - Biochemistry 2023Quote: ... solution and 150 µL water were added to the samples in Eppendorf tubes which were then grinded three times for 30 seconds using a TissueLyser II (Qiagen®) after addition of metal beads ...
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bioRxiv - Cell Biology 2023Quote: ... all samples were diluted to the same RNA concentration and 250 ng of RNA from each sample spiked with 100 ng mouse RNA to control reaction efficiency were reverse transcribed using the miScript II RT kit (Qiagen, 218161) with HiFlex Buffer according to manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... We placed at least 200mg of feces into individual bead tubes from the kit and then homogenized the sample (Tissue Lyser II, Qiagen, Germany) two times for 20 seconds ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were homogenised in QIAzol reagent (1 mL) in a 2 mL safe lock tube with stainless steel beads using a TissueLyser II homogeniser (Qiagen, UK) at 30 Hz until fully homogenised (1-2 min) ...
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bioRxiv - Plant Biology 2023Quote: ... circular leaf punches were collected from the infiltrated leaves and homogenized in 0.25 mM MgCl2 with a TissueLyser II (Qiagen, Toronto, ON). Samples were then serially plated onto LM media containing rifampicin and incubated at 21-24°C overnight ...
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bioRxiv - Evolutionary Biology 2023Quote: Tissue pools were homogenised for 2 min at 30 Hz using 5 mm steel beads on a TissueLyser II instrument (Qiagen, Germany). A volume of 0.2× chloroform (Carl Roth ...
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bioRxiv - Molecular Biology 2023Quote: ... One mL TRIzol was added to all samples and homogenized with stainless steel grinding balls for 2 minutes at 30 Hz using a TissueLyser II bead mill (Qiagen, USA). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... The homogenization was performed using a Tissue-Lyser II with stainless steel grinding balls (2 x 30s at 30 Hz) (Qiagen, USA). After 30 minutes of end-over-end at 5°C ...
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bioRxiv - Neuroscience 2024Quote: The fresh-frozen brain tissue from control and epileptic animals was homogenized with a handheld rotor-stator homogenizer (QIAGEN, TissueRuptor II) in a lysis buffer that contains 0.5% Triton X-100 ...
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bioRxiv - Microbiology 2024Quote: ... followed by rotation of the adapter and ten additional minutes of bead beating using a TissueLyser II (QIAGEN; Cat. No. 85300) using a 2 ml Tube Holder Set (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... samples were thawed and homogenized at a frequency of 26/s for 1 minute in a TissueLyser II (Qiagen, Germantown, MD) and centrifuged at 16,500xg for 5 minutes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... approximately 1 mm3 of tissue (either lungs or intestines) was placed in 750 µL of DMEM medium and homogenized in a TissueLyser II (Qiagen, Hilden, Germany) for 2 min at 25 Hz using 3 mm tungsten beads ...
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bioRxiv - Neuroscience 2019Quote: ... tissue punches were extracted with 500 μL Trizol® RNA reagent and homogenized using a Qiagen TissueLyser II™ (Qiagen, Valencia, CA) for 2-4 min at 20 Hz to ensure thorough homogenization of samples ...
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bioRxiv - Microbiology 2021Quote: ... ethanol-stored flies were rehydrated in water and transferred to 1.5 ml well plates for homogenisation using a bead beater (Qiagen Tissue Lyzer II). Protein was digested using Proteinase K ...
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bioRxiv - Plant Biology 2020Quote: ... Plant material was dried at 60°C overnight and ground to powder using a ball mill (Tissue Lyser II, Qiagen, Venlo, Netherlands). Aliquots of about 1.5-2 mg were transferred into tin capsules (IVA Analysentechnik ...
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bioRxiv - Plant Biology 2020Quote: ... 80-100 mg (fresh weight) moss material from protonema cultures were disrupted with the use of a stainless steel ball in TissueLyser II (Qiagen, Hilden, Germany) for 1 min with an impulse frequency set to 30 ...
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bioRxiv - Microbiology 2020Quote: ... Compost was crushed using mortar and pestle under liquid nitrogen and ground to a fine powder for 1 min at 30 Hz using a tissuelyser II with a steel grinding jar pre-cooled with liquid nitrogen (Qiagen, Venlo, Netherlands). RNA was extracted using a modified method described by Patyshakuliyeva et al ...
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bioRxiv - Plant Biology 2020Quote: ... the root and leaf dry matter were ground into powder by placing the samples into glass vials with three opposing blades and shaking at a frequency of 30 Hz for 10 minutes using a Qiagen TissueLyser II (Qiagen, ML, USA). Ground root and leaf percent N was determined by the Dumas method using the Elementar rapid N exceed analyzer (Elementar Americas Inc ...
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bioRxiv - Cell Biology 2020Quote: Engineered muscles were snap frozen upon collection and TRIReagent® extraction was augmented by mechanical disruption of constructs in a TissueLyser II (Qiagen, UK) for 5 minutes at 20Hz ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Leaf tissue was oven-dried at 55LJ over 24 hours and then homogenized into a fine powder using a TissueLyser II (Qiagen, Hilden, Germany). Between 4-5 mg of homogenized leaf tissue were weighed and placed into a tin capsule (Costech ...
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bioRxiv - Biophysics 2022Quote: ... End-labeled lambda DNA was purified using Qiaex II gel-extraction DNA clean-up kit following the manufactures’ instructions (Qiagen cat# 20021).
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bioRxiv - Neuroscience 2022Quote: ... The tissue samples were then placed in a pre-chilled Eppendorf tube and homogenized with a Qiagen TissueRuptor II (Cat # 9002755) with disposable probes (Qiagen, Cat # 990890). Total RNA extraction was performed using trizol-chloroform extraction followed by column centrifugation using the Invitrogen PureLink™ RNA Mini Kit following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Aqueous homogenates of ovarian tissue samples were created by weighing the tissue and adding 4uL of water per mg of tissue and then using a bead beater (TissueLyser II, QIAGEN; Germantown, MD) to homogenize the sample ...
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bioRxiv - Plant Biology 2021Quote: All reverse transcription (RT) reactions were performed using SuperScript II following the manufacturers’ instructions with 2.5 mM Random Hexamers (Qiagen; Valencia, CA, USA), 100 units SuperScript II and 30 units RNaseOUT (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... 120 mg l-1 sodium pyruvate and 10% FCS (supplemented with 10% Fetal Calf Serum and 1% penicillin–streptomycin) at 300 Hz for two minutes using the Tissuelyser II (Qiagen, Hilden, Germany) and centrifuged to clarify the supernatant ...
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bioRxiv - Immunology 2020Quote: ... 1µg/ml Aprotinin, 1mM PMSF) using 5mm Stainless Beads and TissueLyser II (frequency: 30/sec, duration 2 × 30 sec) (Qiagen, Hilden, Germany). IL-23 was measured using either a mouse IL-23 ELISA (R&D ...
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bioRxiv - Evolutionary Biology 2019Quote: ... with sterile glass beads and beaten for 30 s at full speed in a Qiagen Tissue Lyser II (Qiagen, Hilden, Germany®). After cell lysis ...
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bioRxiv - Microbiology 2019Quote: ... The tube was subjected to bead beating for 3 x 45 seconds at 30 m/s in a TissueLyser II (Qiagen, Hilden, Germany) and placed on ice for 2 mins before repeating ...
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bioRxiv - Genomics 2020Quote: Total RNA was isolated from GALA II subject nasal airway epithelial brushings using the AllPrep DNA/RNA Mini Kit (QIAGEN, Germantown, MD). Whole transcriptome libraries were constructed using the KAPA Stranded mRNA-seq library kit (Roche Sequencing and Life Science ...
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bioRxiv - Microbiology 2021Quote: ... Maize and soybean leaf and root tissue were pulverized for 2-min at a speed of 30 Hz with two 4-mm stainless balls in a TissueLyser II (Qiagen, Venlo, Netherlands). Total DNA was extracted from plant tissues with the OMEGA Mag-Bind Plant DNA Plus kit (Omega Bio-Tek ...