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Citations for Qiagen :
701 - 750 of 978 citations for Scyliorhinin II amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: DNA was isolated by mechanical disruption using glass beads in a TissueLyser II bead mill (Qiagen, Hilden, Germany) (66 ...
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bioRxiv - Physiology 2022Quote: ... The tissue was homogenized for 1 min at 28.5 Hz in a TissueLyser (Qiagen TissueLyser II, Retsch GmbH, Germany) before samples were rotated end over end for 1 hour at 4°C and centrifuged at 18320g for 20 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... A 5 mm stainless steel bead was subsequently used to homogenize the tissue using the TissueLyser II system (Qiagen) for 2x 5 min at 20 hz ...
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bioRxiv - Molecular Biology 2021Quote: The methylation status at the Sox8 promoter was scored for using the EpiTect methyl II PCR kit from QiaGen according to manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... pMo-EGFP-PV-WT plasmid was linearized with ApaI and purified with QIAEX II Gel Extraction Kit (Qiagen, Netherlands). With the linearized plasmid as the template ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA-derived cDNA (which includes cDNA derived from miRNA) was generated using the miScript II RT Kit (Qiagen) using the hiFlex buffer according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... 6 replicates with 4 pupae in each were collected and then homogenized in PBS + 0.05% Triton-X 100 with a tissuelyser II from Qiagen. The TAG levels was determined with a Liquick Cor-TG diagnostic kit (Cormay ...
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bioRxiv - Microbiology 2020Quote: Organ tissue was homogenized in 750 μl Trizol reagent using a Tissuelyser II (spleen and liver) (Qiagen, Germantown, MD) or gentleMACS dissociator system on the RNA setting (Lung) ...
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bioRxiv - Plant Biology 2021Quote: ... Leaf samples were homogenised using a TissueLyser II and RNA was extracted using the RNeasy Plant Mini Kit (Qiagen). Roots were ground with mortar and pestle in liquid N2 and RNA was isolated according to Massey 59 ...
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bioRxiv - Plant Biology 2021Quote: ... 18-20 frozen roots in liquid nitrogen were finely ground using glass beads in a Tissue Lyzer II (Qiagen). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... Target bands (∼420 bp) were cut and eluted using a QIAEX II gel extraction kit (Qiagen, Valencia, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... for 30 min at 37 °C followed by bead-beating with 50 μg 0.1 mm of zirconium beads for 6 min on the Tissuelyzer II (Qiagen) prior to loading onto the Qiacube HT ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 g for 10 minutes at 4°C ...
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bioRxiv - Physiology 2021Quote: ... 10-50 mg tissue was homogenized (1 mM EDTA and 4 mM sodiummetabisulfite, pH 7.4) using a TissueLyser II (Qiagen). Samples where adjusted to the same weight/volume percentage by adding a volume of homogenization buffer and the NA content was measured by ELISA (BA E-5200 ...
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bioRxiv - Plant Biology 2020Quote: ... 200 mg of freeze-dried and ground roots were mixed with 1.6 mL of 1 M perchloric acid and homogenized in a TissueLyser II (Qiagen) in microtubes containing 6 glass beads of 2.8 mm in diameter ...
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bioRxiv - Systems Biology 2020Quote: ... The sealed plates were subjected to 10 minutes of bead beating at 20 Hz using an (Qiagen Tissuelyser II). After bead beating ...
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bioRxiv - Molecular Biology 2020Quote: ... were separated in 1.2% (w/v) low melting agarose gels and the ∼0.6 kb band was purified (QIAEX II Gel Extraction kit, Qiagen). This fragment was used as a template in a second PCR reaction with oligonucleotides VHH-Sfi2 (5′-GTC CTC GCA ACT GCG GCC CAG CCGGCC ATG GCT CAG GTG CAG CTG GTG GA-3’ ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 ng of total RNA was used for establishing the cDNA library with the miScript II RT Kit (Qiagen). qRT-PCR was performed with the SYBR Green I Master kit (Roche Applied Science ...
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bioRxiv - Microbiology 2022Quote: ... Spleen and lymph nodes were disrupted in PBS 0.1% Triton X100 using a stainless bead with the TissueLyser II (Qiagen). The tail-draining sciatic and inguinal lymph nodes were combined before disruption ...
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bioRxiv - Microbiology 2020Quote: ... Cells were mechanically disrupted by bead-beating for 2 × 3 min at 30 Hz (TissueLyser II, Qiagen, Hilden, Germany). The samples were incubated at −80°C for 10 min and at 95°C for 10 min ...
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bioRxiv - Physiology 2022Quote: Frozen liver tissue was manually crushed and 30 mg per sample was homogenized in 1mL of 2:1 chloroform:methanol via a TissueLyser II (Qiagen). Samples were stored at 4 °C overnight with agitation ...
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bioRxiv - Developmental Biology 2019Quote: ... The cDNA was synthesized from total RNA (0.1 – 1.0 µg) with miScript II RT Kit (Qiagen, Germantown, MD, USA). qRT-PCR was performed using miScript SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Pathology 2020Quote: 200 microgram of kidney tissue were homogenized and lysed by 8M urea in 20mM HEPES (pH=8.0) buffer with protease and phosphatase inhibitors using Tissue Lyser II (QIAGEN). Samples were reduced and alkylated followed by MS-grade trypsin digestion ...
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bioRxiv - Physiology 2020Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and the tubes subjected to ten minutes of vigorous shaking at 30 Hz in a TissueLyser II machine (Qiagen). After subsequent DNA binding and washing steps in accordance with the manufacturer’s protocol ...
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bioRxiv - Pathology 2020Quote: ... the total RNA (containing small RNAs) was subjected to miRNA reverse transcription using the miScript II RT Kit (Qiagen), following the manual ...
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bioRxiv - Microbiology 2021Quote: ... The samples were frozen in liquid nitrogen and homogenized in stainless steel grinding jars in a TissueLyser II (Qiagen) for 2 minutes at 30 Hz ...
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bioRxiv - Microbiology 2021Quote: ... snap-frozen in liquid nitrogen and ground to a fine powder using a Qiagen Tissue Lyser II (Qiagen, Germany) at 25 Hz for 60 seconds ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from maternal SI and fetal guts using the Tissue Lyser II (Qiagen, Hilden, NRW, Germany) and RNA extraction kits following manufacturer’s instructions (QIAGEN RNeasy Plus Mini Kit ...
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bioRxiv - Immunology 2020Quote: Lung tissue from individual mice were homogenized in 1 mL of TRIsure reagent with TissueLyser II (Qiagen No. 853000) and as per the manufacturer’s instructions and samples were centrifuged for 15 min at 12,000 x g at 4°C to remove cellular debris ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were homogenized at 25–30 Hz for 5 min using a tissue homogenizer (QIAGEN TissueLyzer II, Hilden, Germany). Samples were swabbed with sterile dual tip swabs (BD swubes ...
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bioRxiv - Physiology 2020Quote: ... and interscapular BAT were placed in ice-cold RIPA buffer and lysated in TissueLyser (TissueLyser II, Qiagen, Vancouver, Canada). The samples were sonicated and centrifuged at 14,000 g for 20 minutes at 4°C ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4C ...
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bioRxiv - Physiology 2021Quote: ... PBS) using a 5 mm stainless steel bead at 30 hertz for 45 seconds in a TissueLyser II (Qiagen). Samples were then centrifuged at 10,000 rcf for 10 minutes at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The total protein fraction was extracted by homogenizing the frozen plant material with metal beads (2.5 mm diameter) in a TissueLyser II (Qiagen). Per fresh sample weight ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen pieces of tissue (~20 mg) were homogenized in 0.5 ml TRIzol in a TissueLyser II (Qiagen, Hilden, Germany) (2 × 2 min at 30 Hz ...
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bioRxiv - Molecular Biology 2020Quote: ... Frozen samples were homogenized with zirconia beads YTZ-4 (AS-ONE, Osaka, Japan) using TissueLyser II (Qiagen, Hilden, Germany), and total RNA was then extracted using the Maxwell 16 LEV Plant RNA Kit (Promega ...
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bioRxiv - Immunology 2021Quote: ... Samples were added to 2 ml ZR BashingBead lysis tube with 0.5 mm beads (Zymoresearch) and tubes arranged in a pre-cooled Tissuelyzer II adapter (Qiagen) and disrupted by shaking at 50 Hz for 25 min ...
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bioRxiv - Genomics 2021Quote: ... Long range PCR amplicons and non-enriched DNA were further purified using the QIAEX II Gel extraction Kit (Qiagen). Due to indispensable loss of HCMV-DNA (>80% ...
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bioRxiv - Genetics 2022Quote: ... snap-frozen kidneys were homogenized and lysed in MagMAX™-96 lysis buffer using a TissueLyser II machine (Qiagen). RNA quality and concentration were determined by a NanoDrop-1000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... and then homogenized tissues in 1 ml PBS containing a penicillin/streptomycin mixture by using a TissueLyser II (Qiagen) at 30-Hz oscillation frequency for 3 min ...
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bioRxiv - Microbiology 2023Quote: ... 25 mg of freeze-dried leaf tissue was ground into a fine powder using two 1.5 mm diameter steel beads in a TissueLyser II (Qiagen) at 30 Hz for 1 minute ...
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bioRxiv - Genetics 2023Quote: ... leaf tissues were collected and homogenized in 400 μL of TPS buffer (100 mM Tris-HCl, 10 mM EDTA, 1 M KCl, pH8.0) using TissueLyser II (Qiagen), followed by incubation for 20 min at 75°C ...
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bioRxiv - Microbiology 2023Quote: ... and the 9.126 kb backbone was gel purified using a Qiaex II Gel Extraction Kit (Qiagen Cat. No. 20051), alongside the PCR product ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with Angiotensin-II (1 μM) alone or in combination with DAPT (10 μM) or siNotch1 (Qiagen) (50 nM ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf discs were ground in 1 mL sterile water with the help of a tissue lyser (QIAGEN, TissueLyser II) using 1.5 mL safe-lock Eppendorf tubs containing three 2.4 mm metal beads ...
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bioRxiv - Biochemistry 2023Quote: ... 5-6 mg of frozen tissue was homogenized in 250 μL ice-cold acidified methanol (0.1% HCl) and 250 μL water washed with hexanes in a bullet blender (TissueLyser II, Qiagen) with 5 mm stainless steel beads (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... and seeds (in situ) were ground using two sterile stainless-steel beads in a Tissue Lyzer II (Qiagen, Germany) for 3×30s at 30Hz ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were extracted from agarose gels after electrophoresis and purified using the QIAEX II Gel Extraction Kits (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... x mg solid matrix were mixed with five times the μl amount of ACN:water (1:1, v/v) and homogenised with a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After a short centrifugation (2 min ...