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Citations for Qiagen :
801 - 850 of 978 citations for Scyliorhinin II amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... Individual females were homogenized in QIAGEN ATL Buffer in 96 well-plates with a sterile glass bead per well on a Tissue Lyser II (QIAGEN) at 23s/f for 2 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... 1pM RT4 was added to the Qiazol before RNA extraction as internal control and 5 uL of total RNA was used as input for reverse transcription reactions using the miScript RT II System (Qiagen) followed by QuantiTec SYBR Green PCR kit (Qiagen) ...
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bioRxiv - Genetics 2019Quote: ... Frozen spores were lysed using cryogenic bead beating with two 5 mm steel beads shaking twice for 45 sec at 25 Hz using TissueLyser II (Qiagen). Lysis buffer was added to the frozen broken spore pellet ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were extracted by disrupting the cells twice with glass beads in the lysis buffer for 45 seconds in a TissueLyser-II (QIAGEN). Immunoblots were performed as previously described [6] ...
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bioRxiv - Immunology 2019Quote: Lung tissue from individual mice were homogenized in 1 mL of TRIsure reagent with TissueLyser II (Qiagen, cat. no. 853000) and as per the manufacturer’s instructions and samples were centrifuged for 15 min at 12,000 x g at 4°C to remove cellular debris ...
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bioRxiv - Microbiology 2020Quote: ... Cells were lysed by boiling (5 min 95°C) followed by bead beating (3mm beads, 30 Hz for 1 min) (TissueLyser II, Qiagen) and sonication bath (3×10 sec at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... After vortexing the samples for 10 s and lysing the tissue with 4 mm glass beads for 1 min at 30 Hz in the TissueLyser II (Qiagen), 400 μL of Tris-HCl pH:7.5 were added and the samples were again mixed for 1 min in the TissueLyser ...
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bioRxiv - Molecular Biology 2020Quote: ... catalysis was measured using a previously described assay (22) in which hexahistidine-tagged PARP-1 was immobilized on Ni(II)-NTA-coated plates (Qiagen) in the presence of NAD+/biotinylated-NAD+ (Trevigen) ...
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bioRxiv - Molecular Biology 2021Quote: ... reverse transcription and quantitative real-time PCR (qRT-PCR) were performed with miScript II RT Kit and SYBR Green PCR Kit respectively (QIAGEN). To quantify α-Syn mRNA levels ...
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bioRxiv - Biochemistry 2020Quote: ... a 5□mm steel bead and polar phase solvents (for a total of 500 µL water and 500 µL MeOH) were added and samples were homogenized using a TissueLyser II (Qiagen) for 5□min at a frequency of 30 times/sec ...
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bioRxiv - Immunology 2020Quote: ... suspended in 1mL PBS supplemented with protease inhibitor and 2mM EDTA and cut into small pieces before being mechanically homogenized with stainless steel beads and TissueLyser II (Qiagen) for two minutes at 3Hz/s ...
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bioRxiv - Microbiology 2020Quote: ... Glass beads (3mm) and 1 mL of sterile water were added for homogenization at 30Hz for 10 minutes (TissueLyser II, Qiagen). Homogenates were diluted with PBS and inoculated onto SAB plates and incubated for 3 days at 30°C ...
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Glutaric aciduria type 3 is a naturally occurring biochemical trait in inbred mice of 129 substrainsbioRxiv - Biochemistry 2020Quote: ... were homogenized in ice-cold RIPA buffer supplemented with protease inhibitor cocktail (∼20 mg tissue per 1 mL lysis buffer) using a TissueLyser II apparatus (Qiagen) and the homogenates were centrifuged at 1,000xg ...
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bioRxiv - Neuroscience 2020Quote: ... Extraction of genomic DNA (gDNA) from 200µL of whole blood or from tissues using the Gentra Puregene kit and Tissue Lyser II (Qiagen, France) was performed accordingly to manufacturer recommendations.
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bioRxiv - Developmental Biology 2020Quote: ... Total RNA was extracted using a QIAGEN RNeasy Lipid Tissue Mini Kit (for whole larvae samples, processed with QIAcube after homogenization with the Tissue Lyser II (Qiagen)) or a QIAGEN RNeasy Micro Kit (for dissected wing discs ...
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bioRxiv - Pathology 2021Quote: Striped dolphin brain and lung samples were submitted to total RNA extraction using the TissueLyser II (QI-AGEN) and highspeed shaking in Eppendorf tubes with stainless steel beads (5 mm diameter, QIAGEN). Homogenates were centrifuged at 14,000 rpm for 3 min to remove tissue debris ...
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bioRxiv - Immunology 2020Quote: ... Organs were homogenized either with a polytron homogenizer (ProScientific Inc., Monroe, CT, USA) or the TissueLyser II (Qiagen No. 85300) at 25 Hz for 2 min twice ...
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bioRxiv - Immunology 2020Quote: ... 50 mM Tris-HCl pH 8.0) with 0.1 μL Protease Inhibitor Cocktail VI (Astral Scientific No. P-1515) using the TissueLyser II (Qiagen No. 85300) at 25 Hz for 2 min twice ...
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bioRxiv - Physiology 2019Quote: Mouse muscles were pulverized in liquid nitrogen and homogenized 2 × 0.5 min at 30 Hz using a TissueLyser II bead mill (Qiagen, USA) in ice-cold homogenization buffer (10% glycerol ...
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bioRxiv - Genetics 2019Quote: RNA was extracted from skin fibroblasts of individual II.4 and a healthy unrelated control using Rneasy RNA kit (Qiagen) then we performed reverse transcription using the iScriptTM cDNA Synthesis Kit (BioRad ...
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bioRxiv - Plant Biology 2019Quote: ... fifty milligrams of fresh leaves in a 2-ml tube was frozen in liquid nitrogen and ground using a Tissuelyser II (Qiagen). The ground sample was extracted with 500 μl of extraction solvent ...
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bioRxiv - Physiology 2019Quote: Muscles were pulverized in liquid nitrogen and homogenized 2 × 0.5 min at 30 Hz using a TissueLyser II bead mill (Qiagen, USA) in ice-cold homogenization buffer (10% glycerol ...
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bioRxiv - Molecular Biology 2019Quote: ... worms were homogenized in PBS (50 μl/adult worm pair or 50 μl/1000 schistosomula) at 4°C using a TissueLyser II (Qiagen), homogenates were incubated overnight with mixing at 4°C and the supernatants collected by centrifugation at 15,000 g for 1 h at 4°C ...
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bioRxiv - Genetics 2020Quote: U8 DNA templates containing a T7 consensus sequence were PCR amplified from human or zebrafish genomic DNA (see Supplementary Table 2 for primer sequences) and purified after agarose gel electrophoresis using a QIAEX II kit (Qiagen). Human and zebrafish U8 snoRNAs were generated using 400-1000ng of template DNA and a mMESSAGE mMACHINE T7 kit (Life Technologies ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were run on an agarose gel and bands of the targeted size were cut and isolated using the QIAEX II Gel Extraction Kit (Qiagen). We successfully amplified sufficient DNA for labelling for scaffolds W005 ...
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bioRxiv - Microbiology 2022Quote: ... Samples initially underwent a mechanical lysis step by adding samples to a Lysing Matrix E tube and placed within a Tissuelyzer II (QIAGEN), which were lysed for 1 minute at 30 Hz ...
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bioRxiv - Synthetic Biology 2021Quote: ... The vector pJPZ11_GP1 was prepared by linearizing it with NdeI following NEB protocol and gel purification with QIAEX II Gel Extraction Kit (QIAGEN). The insert was prepared by PCR with the protocol described in (dx.doi.org/10.17504/protocols.io.bprimm4e) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The tube was then fitted in a 96-well storage plate and secured between the top and bottom plates of TissueLyser II (QIAGEN). The components were then mixed for 10 seconds at 15 Hz followed by 7 seconds at 17 Hz ...
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bioRxiv - Synthetic Biology 2021Quote: ... The tube containing the two phases was then fitted in a 96-well storage plate and secured between the top and bottom plates of TissueLyser II (QIAGEN). The components were then mixed for 10 seconds at 15 Hz followed by 7 seconds at 17 Hz ...
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bioRxiv - Synthetic Biology 2021Quote: ... The tube containing the two phases was then fitted in a 96-well storage plate and secured between the top and bottom plates of TissueLyser II (QIAGEN). The components were then mixed for 10 seconds at 15 Hz followed by 7 seconds at 17 Hz ...
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bioRxiv - Microbiology 2021Quote: ... TIMERI161T T197A was then amplified using the Agilent Gene Morph II kit for a single round of random mutagenesis and PCR products were purified (QIAGEN). These products were used as template DNA in an additional PCR reaction ...
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bioRxiv - Genomics 2020Quote: ... The final library sample was purified from a 2% agarose gel with silica beads (QIAEX II Gel Extraction Kit, Qiagen). 125bp paired-end sequencing was run on an Illumina HiSeq2500 sequencer at the CRG Genomics Core Facility.
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bioRxiv - Microbiology 2021Quote: ... A small portion of the RNA was then reverse transcribed into cDNA using the miScript II RT kit (Qiagen 218160) followed by qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... The specimens were gently and quickly homogenized using a BioMasher II homogenizer (Funakoshi) and mixed with 2 mL of Buffer G2 (QIAGEN), including 200 µg/mL RNase A and 50 µL proteinase K (20 mg/mL) ...
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bioRxiv - Genetics 2021Quote: ... frozen and lyophilised in a 2 ml 96 deep-well plate following which the samples were ground in the TissueLyser II (QIAGEN) using a steel ball in each well for 4-6 minutes at a frequency of 25 Hz ...
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bioRxiv - Genetics 2019Quote: ... in each well of 8-stripped PCR tubes by shaking with a zirconia bead (2 mm in diameter, Nikkato, Japan) in TissuLyser II (Qiagen) for 30 s at 30 Hz ...
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bioRxiv - Molecular Biology 2021Quote: ... at 55°C for 8h followed by 20 min heat inactivation at 80°C and gel purified using the QIAEX II Gel Extraction Kit (Qiagen). The gene fragments were ligated into the backbone using T4 DNA ligase (NEB ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA samples were obtained from dissected tissues from adults or from a pool of embryos or larvae using RNAble solution (Eurobio) and a TissueLyser II system (Qiagen). Total RNA samples were treated with DNAse I (Roche ...
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bioRxiv - Genetics 2022Quote: ... 8 cm of leaf tissue was harvested on ice and then lyophilized using a TissueLyser II (Qiagen, Valencia, CA, USA). Genomic DNA was extracted using a Qiagen DNeasy Plant Mini Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2022Quote: ... mosquitoes were homogenized in 1.7 mL tubes with 50 μL of water and approximately ten 1.0 mm diameter zirconia beads (Thistle Scientific 11079110zx) by shaking at 30 Hz for 2 minutes in a Tissue Lyzer II homogenizer (Qiagen). DNA was extracted using a PureLink Genomic DNA Mini kit (Thermofisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and 150 μL DMEM supplemented with 2% FBS and homogenized using a TissueLyser II (2 cycles at 30 Hz, 1 min, Qiagen). Homogenates were clarified by centrifugation and frozen until plaque assay titration ...
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bioRxiv - Plant Biology 2022Quote: ... The tissue was then ground to a fine powder using 3/16 inch (4.76mm) ball bearings in a Tissuelyzer II (Qiagen, Germany) in the presence of dry ice in the pockets around tube holders ...
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bioRxiv - Plant Biology 2022Quote: ... The lyophilized samples were ground to a fine powder at room temperature using 4.76 mm ball bearings in a Tissuelyzer II (Qiagen, Germany). Following the manual’s instructions ...
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bioRxiv - Physiology 2023Quote: ... small RNA fragment enriched total RNA isolated from each sample was reverse transcribed into cDNA using a miScript II RT Kit (Qiagen). RT-qPCR was performed using miScriptSYBR Green PCR Kit (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: Leaf tissue samples were collected and freeze-dried in a deep-well plate and ground in a TissueLyser II (QIAGEN) for 4-6 minutes at a frequency of 25 Hz ...
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bioRxiv - Physiology 2022Quote: ... They were subsequently organized in metal brackets that were stored at -20°C and homogenized with stainless steel beads at 30 Hz using a TissueLyser II (Qiagen). Samples were lysed twice for one minute ...
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bioRxiv - Plant Biology 2022Quote: ... frozen in liquid nitrogen, ground to a fine powder (3 mm glass beads added to tissue, ground using tissue lyser (TissueLyser II, QIAGEN), 30/second frequency ...
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bioRxiv - Microbiology 2023Quote: ... samples were transferred from DNA/RNA Shield – Lysis Tube to a 2 ml eppendorf and cells lysed using a TissueLyser II (Qiagen) at maximum speed (5 repetitions of 1 min with 1 min on ice between each) ...
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bioRxiv - Plant Biology 2023Quote: Arabidopsis rosettes of 4-week-old plants (Col-0, hos1 and HOS1-GFP/hos1 complementation lines) were finely ground using a TissueLyser II (Qiagen), and proteins were extracted using the IP buffer described in the “Immunoprecipitation assays” (above ...
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bioRxiv - Microbiology 2022Quote: ... transferred to 2 ml lysis matrix B tubes (MPBio) and subjected to bead beating for 15 min at 30 Hz (Tissuelyser II, Qiagen) with RNAse A added ...