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Citations for Qiagen :
851 - 900 of 978 citations for Scyliorhinin II amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 2 h after the light is on) and flash-frozen in liquid nitrogen before powdering with glass beads using a TissueLyser II (Qiagen).
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bioRxiv - Physiology 2023Quote: ... Approximately 25 mg of powdered gastrocnemius were homogenized with pre-chilled metal beads using a TissueLyser II (Qiagen, Hilden, Germany) for 40 seconds at 25 Hz ...
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bioRxiv - Genetics 2023Quote: ... liver samples were homogenized in Qiazol lysis buffer in a TissueLyser II and RNA isolated with RNeasy Mini Kit (Qiagen) following manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... was reverse transcribed into cDNA by miScript RT II Kit using miScript HiSpec Buffer or miRCURY LNA RT Kit (Qiagen). Expression levels of miR-142-5p were analyzed using miScript PCR System or miRCURY LNA SYBR Green PCR Kit (Qiagen) ...
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bioRxiv - Plant Biology 2023Quote: 8-day-old seedlings growing on agar plates in long-day condition were collected and flash-frozen in liquid nitrogen before powdering with metal beads using a TissueLyser II (Qiagen). Total RNA was isolated using the NucleoSpin® RNA Kit (Thermo ...
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bioRxiv - Microbiology 2023Quote: ... fresh 0.35 g/L proteinase K) during two rounds of 3 minutes at a 30Hz/s frequency in a TissueLyser II grinder (Qiagen). Total RNA was converted into complementary DNA (cDNA ...
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bioRxiv - Plant Biology 2023Quote: ... Arabidopsis thaliana frozen leaf tissues were weighed and ground by using two 3 mm stainless steel beads for 3 minutes at 30 Hz with frozen adapters on a TissueLyser II (Qiagen). The resulting frozen powder was dissolved in 650 µL chloroform-methanol (3:7 ...
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bioRxiv - Plant Biology 2023Quote: Frozen tissue was ground into a fine powder using a ⅛” steel ball bearing with 1 min shaking at 25 Hz in a TissueLyser II (Qiagen). Total RNA was extracted from finely ground tissue using TRI reagent (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Prior to RNA extraction individuals were homogenized in 200 μl homogenization buffer including 4 μl 1-Thioglycerol using a TissueLyser II (Qiagen) with a mixture of five 1 mm zirconia (BioSpec Products ...
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bioRxiv - Evolutionary Biology 2022Quote: ... liver tissue was minced to small slices on a clean and cold surface and finally homogenized with the TissueRuptor II device (Qiagen) making use of its maximal settings ...
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bioRxiv - Genomics 2023Quote: ... Swabs were incubated at 37 degrees Celsius for 30 minutes then bead beaten for 6 minutes at 30Hz in a TissueLyser II (Qiagen). The rest of the protocol was followed increasing the volumes to account for the additional lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and 1x antibiotic-antimycotic by a TissueLyser II bead mill with 5-mm stainless steel beads (Qiagen). After brief centrifugation ...
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bioRxiv - Plant Biology 2023Quote: ... leaf and kernel tissue was added to prechilled tubes and disrupted using the Tissuelyser II (Qiagen N.V., Venlo, The Netherlands) for 2 rounds at 30Hz for 1 minute each round ...
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bioRxiv - Synthetic Biology 2023Quote: ... The organs were weighed and lysed in 1 mL Triton X-100 solution (0.1% in PBS) using a TissueLyser II (QIAGEN, Germany) according to the manufacturer protocol ...
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bioRxiv - Bioengineering 2023Quote: Reverse transcription of isolated miRNAs for quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed with a miScript II RT Kit (Qiagen) for selected miRNA targets ...
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bioRxiv - Molecular Biology 2023Quote: ... and class II T7 promoter (transcripts starting with +1A or NAD) were produced by PCR and purified by PCR purification kit (Qiagen) by manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: Genomic DNA was prepared from 500 µl overnight cultures using the peqGold bacterial DNA mini kit following the instruction of the manufacturer with the modification of physically opening cells with the TissueLyser II (Qiagen). Purified genomic DNA was paired end sequenced (2 x 150 bp ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and tissuelysed in 100ul of PBS with about ten 1.0mm diameter zirconia/silica beads (Thistle Scientific # 11079110z) using Qiagen TissueLyser II (Qiagen # 85300) for 2 minutes at 30Hz ...
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Sweetwater: an underrated crude glycerol for sustainable lipid production in non-conventional yeastsbioRxiv - Systems Biology 2023Quote: ... tubes were vortexed for 15 s and submitted to cell disruption for 15-min at 4°C using a TissueLyser II (Qiagen) at 30 Hz ...
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bioRxiv - Neuroscience 2024Quote: ... with 0.9 x tissue mass of lysis buffer (1x RIPA buffer with 2% SDS and 2x protease inhibitor cocktail) and TissueRuptor II (Qiagen) homogenization ...
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bioRxiv - Neuroscience 2024Quote: ... 100 mg of frozen brain was taken per mouse sample and lysed with 0.9 x tissue mass of lysis buffer (1x RIPA buffer with 2% SDS and 2x protease inhibitor cocktail) and was homogenised using a TissueRuptor II (Qiagen). Zebrafish larvae were similarly extracted ...
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bioRxiv - Plant Biology 2024Quote: ... were harvested at the bolting stage using liquid nitrogen and ground to powder form using tissue lyser (Tissuelyser II, Retsch, Qiagen) at 30 frequency/ s for 2 mins and lyophilised overnight ...
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bioRxiv - Microbiology 2023Quote: ... preceded by a 10-minute bead-beating step at 30 Hz in 2 ml e-matrix tubes (MP Biomedical, USA) using a Tissuelyser II (Qiagen). Molarity and fragment-length distribution of the extracts were measured using a Tapestation ...
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bioRxiv - Cancer Biology 2023Quote: SCC25 tumours from the co-injection experiment were dissected at day 28 and homogenized in lysis buffer from the Qiagen AllPrep mini kit using Tissue Lyzer II (Qiagen). Total RNA was isolated using Qiagen AllPrep mini kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: NHEJ and HR reporter constructs 38 were digested with NheI restriction enzyme and purified with the QIAEX II gel extraction kit (QIAGEN). The same plasmid DNA preparation was used for generating all reporter cell lines of the studied species ...
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bioRxiv - Microbiology 2023Quote: ... tissues were placed in 300μL of sterile PBS containing sterile glass beads and mechanically lysed at a frequency of 20 shakes per seconds for 3 minutes in a TissueLyser II (Qiagen). Negative controls consisted of tubes containing PBS and beads but no sample ...
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bioRxiv - Cancer Biology 2023Quote: ... NHEJ reporter plasmid was digested with I-Sce1 for 6h and purified using a QIAEX II Gel Extraction Kit (QIAGEN). Exponentially growing cells were transfected using an Amaxa nucleofector with the U-023 program ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was extracted using the NucleoSpin RNA II kit (Machery & Nagel) and the cDNA were synthesized using the QuantiTect Reverse Transcription Kit (Qiagen). cDNA samples were run in triplicates and experiments were repeated with independently isolated RNA samples ...
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bioRxiv - Genomics 2023Quote: ... The amplicon library pools were isolated based on size by gel electrophoresis using a 2% agarose gel and then purified using QIAEX II Gel Extraction Kit (QIAGEN) and using 30uL of QIAEX II beads for each sample ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Approximately 50 mg of tissue was homogenised in 500 µl phosphate-buffered saline (PBS) for 2 min at 30 Hz using 5 mm steel beads on a TissueLyser II instrument (Qiagen). Total nucleic acids were extracted using the Nucleo Mag Vet Kit (Macherey & Nagel ...
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bioRxiv - Neuroscience 2024Quote: ... were added to each tube and samples were homogenized for 2×90s at 20 Hz using a TissueLyser II (Qiagen). Next ...
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bioRxiv - Microbiology 2024Quote: ... one fecal pellet per procedure was placed in a 2 mL round-bottom tube containing a 0.5 cm diameter stainless steel bead and 500 µL of sterile phosphate-buffered saline (PBS) and agitated at 1/30s using a TissueLyser II (Qiagen) for 60 seconds ...
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bioRxiv - Microbiology 2024Quote: ... mosquitoes were homogenized in 500 μl ice-cold 1X Phosphate Buffered Saline buffer with two ice-cold steel bearing balls (3 mm diameter, LOUDET) using a TissueLyser II (Qiagen) and clarified through centrifugation ...
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bioRxiv - Plant Biology 2023Quote: ... samples were flash-frozen in liquid nitrogen immediately prior to grinding and ground using 3 mm glass beads at 30 Hz for 3 min in a TissueLyser II (Qiagen) to a fine powder ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 1 mL phenol:chloroform:isoamyl alcohol (25:24:1 at pH 8) at 70°C for 12 min and bead beating for 2 min (Tissue Lyser II, Qiagen). The mixture was centrifuged at 4°C for 3 min at maximum speed and the aqueous phase was transferred to a new reaction tube ...
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bioRxiv - Microbiology 2024Quote: ... were combined from each individual and minced in a combination of 300 µL of body fluid and 300 µL blood using a TissueLysser II (Qiagen). In addition ...
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bioRxiv - Immunology 2024Quote: ... and brain) homogenates collected from mice at 5 dpi and 5 dpc were generated using the Tissue Lyzer II (Qiagen). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... whereas 30 mg of lung tissue was homogenized in 600 μl RLT-buffer containing β–mercaptoethanol for 30 sec at 15 Hz using the TissueLyser II (Qiagen).
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bioRxiv - Evolutionary Biology 2020Quote: ... was ground to small pieces by one steel ball (Ø 4 mm) in a 2 ml Eppendorf tube with Tissue Lyzer II (Qiagen, Hilden ...
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bioRxiv - Neuroscience 2022Quote: ... and the tissue was homogenized with a 5 mm tungsten carbide bead in a bead mill homogenizer (Qiagen, Tissue Lyser II) at 30 Hz for 1.5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... followed by RNA extraction with TRIzol using Tissue Lyser II (30 Hz frequency for 2 min with 2 cycles, Qiagen, Germany). Equal amounts of RNA from each sample were reverse transcribed in 20 μl to produce cDNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... embryo pellets were homogenized by cryo-milling after addition of a 2.5 or 5 mm steel bead using a tissue lyser (QIAgen TissueLyser II) at 25 Hz for 15 seconds 3-6 times ...
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bioRxiv - Biochemistry 2020Quote: ... Cell pellets were powderized by cryomilling after addition of a 2.5 mm steel bead using a tissue lyser (QIAgen TissueLyser II) 3 times at 25 Hz for 30 seconds ...
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bioRxiv - Molecular Biology 2022Quote: ... In all samples disruption occurred in 1 min intervals to avoid heat generation derived from the TissueRuptor II (catalogue #9002755, Qiagen, MD)29.
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bioRxiv - Evolutionary Biology 2022Quote: ... 10-20 mg of silica-dried stem or leaf tissue was ground with a Qiagen TissueLyser II (Qiagen, Melbourne, Vic., Australia). DNA was extracted using the DNeasy 96 Plant Kit or DNeasyPlant Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... with 1 min rest on ice in between each round of bashing) on a TissueLyser II beadmill (Qiagen, Valencia, CA, USA), then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter ...
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bioRxiv - Genetics 2019Quote: ... The whole body of a single insect was homogenized in a PCR well containing 50 μl of the Lysis & Binding Solution and zirconia beads (ø 2 mm; Nikkato) in TissueLyser II (QIAGEN) at 25 Hz for 30 s ...
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bioRxiv - Systems Biology 2019Quote: ... For improving the lysis step proteinase k and lysozyme were added to the lysis solution and the samples were homogenized using Tissue lyser II (Qiagen S.r.l.). The concentration and purity of RNA were analyzed using a NanoDrop ND-1000 (Thermo Fisher Scientific ...
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rpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencingbioRxiv - Microbiology 2019Quote: ... and were subjected to three cycles of grinding (1 minute, at 30 Hz followed by 1 minute without agitation) in a TissueLyser II apparatus (Qiagen, France). The solutions obtained from crushed nematodes or the contents of G ...
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rpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencingbioRxiv - Microbiology 2019Quote: ... IJs were crushed by three cycles of mechanical grinding (2 minutes at 30 Hz followed by 1 minute without agitation) in a TissueLyser II apparatus (Qiagen, France). We added 2 µL of Ready-Lyse Lysozyme Solution (Epi-centre ...