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Citations for Qiagen :
601 - 650 of 1720 citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from the lysate using the DNeasy® Plant Mini Kit (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted from RLT Plus lysates using the RNeasy Plus Mini Kit (QIAGEN, #74136), including on-column DNase treatment using the RNase-Free DNase set (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... Viral nucleic acids were then isolated using the Qiagen QIAamp MinElute Virus Spin Kit without the use of AW1 buffer or carrier RNA (Qiagen, Valencia, CA, USA). Random hexamers were used to prime cDNA synthesis (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in 384 well plates on an Applied Biosystems 7900HT Fast Real-Time PCR system using the QuantiTect Virus Kit (Qiagen, Redwood City, CA) and SARS-CoV-CDC RUO primers and probes (Integrated DNA Technologies (IDT) ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acids were extracted at CNM using either QIAamp MinElute Virus Spin (DNA) or QIAamp Viral RNA Mini kits (Qiagen, Germantown, MD, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were then processed for RNA and DNA extraction using AllPrep DNA/RNA Mini kit (Qiagen), with Complete Protease Inhibitor (Roche ...
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bioRxiv - Molecular Biology 2020Quote: RNA extractions from frozen lysates were performed on the same day using RNeasy Mini columns (Qiagen). Recovered total RNA was quantified by Qubit and quality was assessed using an Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate was cleared by centrifugation and the soluble fraction was applied to Ni-NTA resin (Qiagen) and incubated for 1.5 h ...
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bioRxiv - Microbiology 2019Quote: ... RNA was then isolated from cleared lysate using the manufacture’s protocol (#74106 RNeasy Mini Kit Qiagen).
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bioRxiv - Genetics 2020Quote: DNA/RNA extraction was performed from cell lysates using the AllPrep DNA/RNA Micro Kit (Qiagen). For initial optimisation experiments using an HPRT gRNA-containing RNP (or non-targeting control) ...
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bioRxiv - Genetics 2020Quote: ... Total DNA was isolated from the cell lysates using the QIAquick® PCR Purification Kit (QIAGEN). The following primers used were designed to the lucia DNA sequence for amplification of the reporter gene (JE041 ...
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bioRxiv - Microbiology 2021Quote: ... Toxins were then purified from bacterial cell lysates through affinity chromatography on a Nickel-resin (Qiagen), ion exchange ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate from bacterial expression was passed over a column with Streptactin XT Superflow resin (Qiagen). After incubation ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation and the supernatant was applied to Ni NTA-agarose (Qiagen), which was washed with the same buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate fraction was bound in batch to nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The resin was washed extensively with 20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate fraction was bound in batch to nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The resin was washed extensively with 20 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acids were isolated from cell lysates or lung homogenates using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The clarified lysate was then loaded onto an open column containing pre-equilibrated Ni-NTA (Qiagen). The column was washed with 20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysate was then transferred to the RNeasy spin column provided in the RNeasy kit (Qiagen). The manufacturers protocol was then followed for the rest of the isolation process.
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bioRxiv - Cell Biology 2020Quote: ... The lysate was centrifuged and the supernatant was load onto a Ni-NTA affinity column (Qiagen). Proteins were eluted with elution buffer (20 mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was first purified on a Ni–nitrilotriacetic acid (NTA) column (QIAGEN, Valencia, CA). The eluate was further purified on glutathione–Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was purified over Ni-NTA affinity resin according to the manufacturer’s recommendation (Qiagen). Eluted proteins were pooled and dialyzed overnight at 4°C into 50 mM Na2HPO4 pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... The bacterial lysates were mixed for 1 h with 5 ml of Ni-NTA resin (Qiagen) that had been equilibrated with buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysates were spun on the QIashredder column and RNA extracted using the RNeasy kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... and the supernatant of the bacterial lysate was loaded to a disposable column (Qiagen, Hilden, German) pre-packed with glutathione agarose (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified lysate was applied to two tandemly-connected 5 ml Ni-NTA Superflow cartridges (Qiagen); the cartridges were washed with buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... mouse organ lysates and human primary cell supernatant samples were prepared in RNeasy Mini Kit (Qiagen) lysis buffer RLT (400μl) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate was cleared by centrifugation and then applied to a column of Ni-NTA resin (Qiagen). The column was washed with 10 column volumes (CVs ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was isolated from Trizol/cell lysate mixture by using RNAeasy Mini Kit (Qiagen #74104). Total RNA was sequenced on Illumina NextSeq using Illumina TruSeq Stranded Total RNA library Prep and paired-end sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... Further these lysates were incubated overnight at 4°C with prewashed Ni-NTA beads (Qiagen, 30210).Sequential washes with wash buffer 1-4 ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted and purified from the lysate using the DNeasy Blood and Tissue Kit (Qiagen). The DNA from the sediments was extracted from 2 g using a sodium phosphate buffer and concentrated into 50 KDa amicon filters ...
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bioRxiv - Microbiology 2023Quote: ... total RNA of infectious cell lysate was extracted using an RNeasy Mini Kit (QIAGEN, Hilden, Germany). RT–PCR was performed using a Superscript III One-Step RT-PCR kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was clarified by centrifugation and incubated with 1 mL Ni-NTA resin (Qiagen). The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Microbiology 2023Quote: ... Lysate supernatants were then passed through chromatography columns containing Ni-NTA agarose resin (reference: 30210; Qiagen). The bound 6x His-tagged toxins were eluted with elution buffer (50 mM NaH2PO4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was passed three times though 2mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen) in a gravity flow column and then washed with 300mL of wash buffer A (25 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were loaded onto a 1 mL Ni-NTA Superflow column (Qiagen, Valencia, CA, USA) that had been equilibrated with 10 column volumes of wash 1 buffer ...
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bioRxiv - Biochemistry 2024Quote: ... and the clarified lysate was applied to a column of Ni-NTA resin (Qiagen and ThermoFisher) preequilibrated in wash buffer 1 (50 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and clarified lysate was loaded onto a Strep-Tactin column (QIAGEN). The column was washed with 20 CVs of wash buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... 500 mM KCl and fusion proteins were purified from clarified lysate through consecutive Ni-NTA (Qiagen) and HiTrap Q HP (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... 500 mM KCl and the fusion proteins purified from clarified lysate through consecutive Ni-NTA (Qiagen) and HiTrap Q HP (GE Healthcare ...
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bioRxiv - Bioengineering 2023Quote: ... Total RNA (tRNA) from the cell lysate were extracted immediately using Qiagen RNeasy Microkit (Qiagen, Germany) before reverse transcription into cDNA using QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: ... The cell lysates were then obtained by adding 350 µL of cell lysis buffer (Qiagen, Germany) to the centrifuged cells ...
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bioRxiv - Molecular Biology 2024Quote: ... The fusion protein in the lysate was affinity-purified by Ni-NTA agarose beads (Qiagen, 30210) capturing HIS tag or by glutathione sepharose 4B beads (Amersham ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were incubated at 37°C for 15 minutes following addition of 2µg RNase A (Qiagen). 1pmol ssDNA substrate 5’ DY782 ATTATTATTATTATTATTATTTCATTTATTTATTTATTTA-3’ (Eurofins ...
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bioRxiv - Developmental Biology 2021Quote: ... oviduct tissue was manually disrupted and the lysate was spun through a QIAshredder column (Qiagen, cat#: 79656) to fully homogenize ...
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bioRxiv - Bioengineering 2021Quote: The soluble fraction of the cell lysate was mixed with Ni2+-nitrilotriacetic acid agarose beads (Qiagen, USA), and the His6-tagged recombinant proteins were purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation and the supernatant loaded on Ni-NTA Superflow affinity resin (Qiagen). The resin was washed with wash buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation and the supernatant loaded on Ni-NTA Superflow affinity resin (Qiagen). The resin was washed with wash buffer (50 mM HEPES/NaOH pH 7.2 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified by centrifugation and the supernatant loaded on Ni-NTA Superflow affinity resin (Qiagen). The resin was washed with wash buffer (50 mM HEPES/NaOH pH 7.2 ...