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Citations for Qiagen :
801 - 850 of 1806 citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... the supernatant of the bacteria lysate was transferred into new 50 ml Falcon tubes and incubated with Ni-NTA (Qiagen) at 4 °C for 30 min ...
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bioRxiv - Bioengineering 2022Quote: ... Total RNA was isolated from the lysate using on-column digestion of genomic DNA via RNeasy Micro Kit (CC# 74004; Qiagen). RNA quality was assessed using High sensitivity RNA TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Genomics 2022Quote: ... The mixture was then centrifuged for 20 min at 12 000 x g and the clarified lysate transferred to an appropriate buffer QBT-equilibrated QIAGEN Genomic-tip 100/G column (Qiagen), after which the column was washed three times with 7 ml Buffer QC and HMW DNA was eluted with 5 ml Buffer QF ...
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bioRxiv - Neuroscience 2022Quote: ... iPS-NES cells were plated in 6-well plates and lysates were collected in RNAprotect® Cell Reagent (QIAGEN, #76526) to stabilize the RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... the clarified lysate was diluted into 1x Ni-NTA binding buffer and bound to equilibrated Ni-NTA resin (Qiagen, 30210) for 20 minutes at 4°C with end-over-end mixing ...
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bioRxiv - Biochemistry 2024Quote: ... Lysed cells were sedimented by centrifugation at 20,000 RCF at 4 °C for 30 min and the lysate was subsequently loaded onto pre-equilibrated Ni-NTA agarose resin (Qiagen) at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... The resulting lysate was then made up to 3 mL with QIAzol Lysis Reagent and mixed thoroughly before 1 mL lysate aliquots were processed using RNeasy Lipid Tissue Kit 74804 (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: BC3 cells were treated with indicated chemicals for 65h and total DNA was purified from cell lysates using the FlexiGene DNA kit (Qiagen). Intracellular viral DNA was measured by real-time quantitative (q ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was isolated from cell lysates or the conditioned media using the RNeasy Plus Micro kit (Qiagen, Hilden Germany) or QIAamp Viral RNA kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... We could not get a PCR product for the R escaper and therefore extracted the total DNA from the escaper and the ancestor λ-vir phage lysate after enrichment for high phage titer (> 107 pfu/ mL) DNA with the DNeasy blood and tissue kit (QIAGEN), starting from the Proteinase-K treatment step to lyse the phages 92 ...
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bioRxiv - Microbiology 2023Quote: ... The cleared lysate was applied to an affinity chromatog-raphy column containing Ni-nitrilotriacetic acid (NTA) su-perflow resin (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Systems Biology 2023Quote: ... Lysis was performed using 350 µL of the provided lysis buffer and the lysate was passed through a QIAshredder column (QIAGEN). RNA from the lysate was obtained by following the manufacturer’s protocol and quantified using a Nanodrop 2000 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... The two lysates were then combined and cleared by centrifugation prior to application onto a Ni-NTA Agarose column (Qiagen) equilibrated in buffer L lacking protease inhibitor cocktail ...
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bioRxiv - Biochemistry 2023Quote: ... Filters were incubated at 55°C for approximately 4 h and the resulting lysates were purified with the DNeasy kit (Qiagen) using a slightly modified protocol49 ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatants were separated from lysates and incubated with affinity beads at 4°C overnight (His beads: Ni-NTA from QIAGEN; GST beads ...
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bioRxiv - Biochemistry 2024Quote: The periplasmically located VC0430 that was used for ESI-MS analysis was purified by applying the clarified lysate to N-NTA resin (Qiagen), washing the resin with Wash Buffer (WB ...
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bioRxiv - Biochemistry 2023Quote: ... and the complex of RAD51 and MBP-BRCA2 BRC4 was purified from clarified lysate through consecutive amylose and Ni-NTA (Qiagen) affinity chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL from the supernatant and cell lysates were applied on a nylon membrane and two different antibodies (an HRP-conjugated anti-His tag from Qiagen and an HRP-conjugated anti-strep tag from Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from the lysate per manufacturer instructions using a AllPrep DNA/RNA mini kit (Qiagen, Valencia, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... equal amounts (200 µl) of FruK and Cra cell lysates were mixed with 100 µl of nickel-nitrilotriacetic acid beads (Qiagen). After overnight incubation at 4°C with gentle rotation ...
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bioRxiv - Microbiology 2023Quote: ... The cytoplasmic lysate was collected and resuspended with 350 µL of RLT+ buffer from RNeasy Plus kit (Qiagen, Hilden, Germany). Then ...
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bioRxiv - Physiology 2024Quote: ... A fraction (10%) of the total lysate volume was mixed with 9 volumes of QIAZOL (Cat. # 79306; QIAGEN, Hilden, Germany) and frozen at -80°C ...
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bioRxiv - Plant Biology 2024Quote: ... Purification under native conditions was done by using the cleared BL21(DE3) lysate and Ni2+-NTA agarose (Qiagen, Hilden, Germany) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: Total RNA has been extracted from cell lysates by using the RNeasy Plus Mini Kit (cat. 74136, Qiagen, Hilden, Germany). The total cDNA was obtained by reverse transcription using random primers from Invitrogen (ref ...
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bioRxiv - Pathology 2024Quote: Total RNA was extracted from cell lysates using EZNA RNA extraction kit I or from blood samples using QIAamp Viral RNA Mini Kit (Qiagen). Absolute quantification of positive strand gRNA was performed through one-step RT-qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested 72 h post-infection and the lysate was loaded on pre-equilibrated Ni-NTA agarose (QIAGEN #30210). 6xHis-NSUN2 ...
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bioRxiv - Microbiology 2024Quote: ... DNA was isolated from the mitochondrial lysate obtained from the gastrocnemius muscle tissue of the mice using the DNeasy Blood and Tissue kit (Qiagen). 1 μg/μl of the extracted DNA was converted to single-stranded DNA by incubating the sample at 95°C for 5 mins ...
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bioRxiv - Immunology 2024Quote: SARS-CoV-2 specific T cell immune responses were evaluated by QuantiFERON SARS-CoV-2 (Qiagen) [36] ...
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bioRxiv - Microbiology 2019Quote: ... containing 2 ml microcentrifuge tubes (QIAGEN) in which samples were placed ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of Buffer EB (Qiagen), and 30 μL of Hybridization Enhancer ...
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bioRxiv - Microbiology 2019Quote: ... 2 µg/mL RNase A (QIAgen), 1/200 (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mL Ni-NTA resin (Qiagen) was used for clarified cell lysate from 1 L bacterial culture ...
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bioRxiv - Microbiology 2022Quote: ... 2) the DNeasy PowerWater Kit (Qiagen), which is optimized for the isolation of genomic DNA from filtered water samples ...
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bioRxiv - Genomics 2023Quote: ... 2 µl 10x Reaction Buffer (Qiagen), 0.2 µl dNTP mix (10 µM) ...
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bioRxiv - Genomics 2023Quote: ... 2 µl 10x Reaction Buffer (Qiagen), 0.2 µl dNTP mix (10 µM) ...
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bioRxiv - Microbiology 2024Quote: ... 2 volumes of RNA protect* (Qiagen) was added to cultures prior centrifugation (10 min 12,000 g ...
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bioRxiv - Molecular Biology 2023Quote: ... and DNase I (2 mg (Qiagen) were dissolved in 30 mL of
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bioRxiv - Biophysics 2021Quote: ... Next the lysate was clarified by centrifugation and the soluble fraction was incubated with equilibrated Ni-NTA resin (Qiagen cat#4561) for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleared lysates were applied to RNeasy silica columns and on-column DNase digestion was performed using RNase-Free DNase Set (QIAGEN, 79254) to remove DNA in silica membrane ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was isolated from cell lysate by phenol::chloroform extraction and spin-column (miRNAeasy kit, Qiagen, San Diego, CA, USA), quantified by spectrophotometry and assessed for quality using the Agilent 2100 bioanalyzer (Agilent Technologies ...
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bioRxiv - Genomics 2019Quote: Isolation of total mRNA for RNA-seq library preparation: Total RNA was isolated from the lysate using miRNeasy Mini Kit (Qiagen #217004) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Proteins (as well as DNA and RNA) were then extracted from the lysate using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germany) following manufacturers instructions ...
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bioRxiv - Immunology 2020Quote: ... Proteins (as well as DNA and RNA) were then extracted from the lysate using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germany) following manufacturers instructions ...
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bioRxiv - Bioengineering 2020Quote: ... and the same amount of lysate from each tumor was used to extract total RNA with the RNeasy Mini Kit (Qiagen, 74104) followed by reverse transcription using the same amount of template RNA ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was extracted from the hiPSCs and hiPSCs-derived neurons lysates using the miRNeasy mini kit (reference 217004, Qiagen, Germany). For each sample ...
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bioRxiv - Developmental Biology 2019Quote: ... A magnetic rack was used to remove the beads and 15 µl of each pre-cleared lysate was collected and added to 200 µl RLT buffer per sample (Qiagen, 74034) to serve as the input samples ...
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bioRxiv - Immunology 2020Quote: ... Pellets were lysed by vortexing for 1 minute in 350uL cold supplemented RLT buffer (RLT + β-MeOH) at 4°C and lysates homogenized using QIAshredder columns (Qiagen). RNA was then extracted from these samples using the RNeasy Plus Micro kit (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... was added to the lysed clot and mixed before transferring 500ul of the lysate to a 2ml tube and run on the QIA Symphony using the QIA Symphony DSP DNA Midi kit (Qiagen™). The protocol was a customised BC 400 protocol and DNA was eluted into 100ul ...
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bioRxiv - Microbiology 2020Quote: ... Lysates containing 6xHis-MBP-OmpW and 6xHis-MBP-EF-Tu were incubated on amylose resin (New England Biolabs, E8021L) (Qiagen, 1018244) and bound proteins were eluted in lysis buffer lacking EDTA and DTT but containing 10 mM maltose ...
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bioRxiv - Cell Biology 2023Quote: ... 200μl chow fed liver lysate or 300μl WD-fed liver lysate was transferred to a 15ml tube for genomic DNA extraction using the Blood & Cell Culture DNA Midi Kit (Qiagen #13343) according to the manufacturer’s protocol ...