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Citations for Qiagen :
751 - 800 of 1720 citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: A cell pellet corresponding to 20-25 OD600 units was lysed with glass beads and RNA was isolated from the cell lysate using the RNeasy Mini Kit and RNase-free DNase Set (both Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Total RNA was extracted from sputum cell pellets or bronchial epithelial cell lysates stored in RLT buffer (RNeasy kit, Qiagen) and 2μg was used for cDNA synthesis (Omniscript RT kit) ...
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bioRxiv - Biochemistry 2019Quote: ... and the lysate was mixed gently with 4 ml (50% slurry) of nickel-nitrilotriacetic acid (Ni-NTA)-agarose resin (Qiagen) at 4°C for 1 h ...
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bioRxiv - Genomics 2021Quote: ... Nucleic acids were purified from the lysates using the Qiagen AllPrep DNA/RNA mini kit (Qiagen Inc., Valencia, CA, USA), quantified using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Purification of p53-(1-73) from the clarified cell lysate was accomplished using a Nickel Nitrilotriacetic Acid (Ni-NTA, Qiagen) column purification and 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris was removed by centrifugation at 20000 g for 40 min at 4 °C and the lysate incubated with Ni-NTA Agarose Beads (Qiagen). After washing ...
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bioRxiv - Cancer Biology 2020Quote: ... we captured poly-adenylated mRNA from cell lysates using a 96-well plate with oligo(dT) grafted to the inner walls of each well (Qiagen). Next ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA was extracted from cell lysates using the RNase Mini Kit where genomic DNA was removed using the RNase-Free DNase Kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were passed five times through a 23G needle before proceeding to RNA extraction using RNeasy Qiagen Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysate was centrifuged at 10,000g for 45 minutes and the supernatant was loaded onto Ni-NTA column (Qiagen, Germany). The unbound protein was washed with washing buffer (50 mM Tris buffer pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Full-length his-tagged ySgt2 and cysteine mutants were separated from the lysate by batch incubation with Ni-NTA resin (Qiagen) at 4°C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatant of the bacteria lysate was transferred into new 50 ml Falcon tubes and incubated with Ni-NTA (Qiagen) at 4 °C for 30 min ...
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bioRxiv - Bioengineering 2022Quote: ... Total RNA was isolated from the lysate using on-column digestion of genomic DNA via RNeasy Micro Kit (CC# 74004; Qiagen). RNA quality was assessed using High sensitivity RNA TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Genomics 2022Quote: ... The mixture was then centrifuged for 20 min at 12 000 x g and the clarified lysate transferred to an appropriate buffer QBT-equilibrated QIAGEN Genomic-tip 100/G column (Qiagen), after which the column was washed three times with 7 ml Buffer QC and HMW DNA was eluted with 5 ml Buffer QF ...
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bioRxiv - Neuroscience 2022Quote: ... iPS-NES cells were plated in 6-well plates and lysates were collected in RNAprotect® Cell Reagent (QIAGEN, #76526) to stabilize the RNA ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was isolated from cell lysates or the conditioned media using the RNeasy Plus Micro kit (Qiagen, Hilden Germany) or QIAamp Viral RNA kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: Total RNA has been extracted from cell lysates by using the RNeasy Plus Mini Kit (cat. 74136, Qiagen, Hilden, Germany). The total cDNA was obtained by reverse transcription using random primers from Invitrogen (ref ...
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bioRxiv - Microbiology 2023Quote: ... We could not get a PCR product for the R escaper and therefore extracted the total DNA from the escaper and the ancestor λ-vir phage lysate after enrichment for high phage titer (> 107 pfu/ mL) DNA with the DNeasy blood and tissue kit (QIAGEN), starting from the Proteinase-K treatment step to lyse the phages 92 ...
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bioRxiv - Microbiology 2023Quote: ... The cleared lysate was applied to an affinity chromatog-raphy column containing Ni-nitrilotriacetic acid (NTA) su-perflow resin (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Systems Biology 2023Quote: ... Lysis was performed using 350 µL of the provided lysis buffer and the lysate was passed through a QIAshredder column (QIAGEN). RNA from the lysate was obtained by following the manufacturer’s protocol and quantified using a Nanodrop 2000 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... The two lysates were then combined and cleared by centrifugation prior to application onto a Ni-NTA Agarose column (Qiagen) equilibrated in buffer L lacking protease inhibitor cocktail ...
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bioRxiv - Biochemistry 2023Quote: ... Filters were incubated at 55°C for approximately 4 h and the resulting lysates were purified with the DNeasy kit (Qiagen) using a slightly modified protocol49 ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatants were separated from lysates and incubated with affinity beads at 4°C overnight (His beads: Ni-NTA from QIAGEN; GST beads ...
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bioRxiv - Biochemistry 2024Quote: The periplasmically located VC0430 that was used for ESI-MS analysis was purified by applying the clarified lysate to N-NTA resin (Qiagen), washing the resin with Wash Buffer (WB ...
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bioRxiv - Biochemistry 2023Quote: ... and the complex of RAD51 and MBP-BRCA2 BRC4 was purified from clarified lysate through consecutive amylose and Ni-NTA (Qiagen) affinity chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL from the supernatant and cell lysates were applied on a nylon membrane and two different antibodies (an HRP-conjugated anti-His tag from Qiagen and an HRP-conjugated anti-strep tag from Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from the lysate per manufacturer instructions using a AllPrep DNA/RNA mini kit (Qiagen, Valencia, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... equal amounts (200 µl) of FruK and Cra cell lysates were mixed with 100 µl of nickel-nitrilotriacetic acid beads (Qiagen). After overnight incubation at 4°C with gentle rotation ...
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bioRxiv - Microbiology 2023Quote: ... The cytoplasmic lysate was collected and resuspended with 350 µL of RLT+ buffer from RNeasy Plus kit (Qiagen, Hilden, Germany). Then ...
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bioRxiv - Biochemistry 2024Quote: ... Lysed cells were sedimented by centrifugation at 20,000 RCF at 4 °C for 30 min and the lysate was subsequently loaded onto pre-equilibrated Ni-NTA agarose resin (Qiagen) at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... The resulting lysate was then made up to 3 mL with QIAzol Lysis Reagent and mixed thoroughly before 1 mL lysate aliquots were processed using RNeasy Lipid Tissue Kit 74804 (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the clarified lysate was diluted into 1x Ni-NTA binding buffer and bound to equilibrated Ni-NTA resin (Qiagen, 30210) for 20 minutes at 4°C with end-over-end mixing ...
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bioRxiv - Microbiology 2024Quote: BC3 cells were treated with indicated chemicals for 65h and total DNA was purified from cell lysates using the FlexiGene DNA kit (Qiagen). Intracellular viral DNA was measured by real-time quantitative (q ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 specific T cell immune responses were evaluated by QuantiFERON SARS-CoV-2 (Qiagen) [36] ...
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bioRxiv - Microbiology 2019Quote: ... containing 2 ml microcentrifuge tubes (QIAGEN) in which samples were placed ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of Buffer EB (Qiagen), and 30 μL of Hybridization Enhancer ...
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bioRxiv - Microbiology 2019Quote: ... 2 µg/mL RNase A (QIAgen), 1/200 (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mL Ni-NTA resin (Qiagen) was used for clarified cell lysate from 1 L bacterial culture ...
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bioRxiv - Microbiology 2022Quote: ... 2) the DNeasy PowerWater Kit (Qiagen), which is optimized for the isolation of genomic DNA from filtered water samples ...
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bioRxiv - Genomics 2023Quote: ... 2 µl 10x Reaction Buffer (Qiagen), 0.2 µl dNTP mix (10 µM) ...
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bioRxiv - Genomics 2023Quote: ... 2 µl 10x Reaction Buffer (Qiagen), 0.2 µl dNTP mix (10 µM) ...
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bioRxiv - Microbiology 2024Quote: ... 2 volumes of RNA protect* (Qiagen) was added to cultures prior centrifugation (10 min 12,000 g ...
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bioRxiv - Molecular Biology 2023Quote: ... and DNase I (2 mg (Qiagen) were dissolved in 30 mL of
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bioRxiv - Biophysics 2021Quote: ... Next the lysate was clarified by centrifugation and the soluble fraction was incubated with equilibrated Ni-NTA resin (Qiagen cat#4561) for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleared lysates were applied to RNeasy silica columns and on-column DNase digestion was performed using RNase-Free DNase Set (QIAGEN, 79254) to remove DNA in silica membrane ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was isolated from cell lysate by phenol::chloroform extraction and spin-column (miRNAeasy kit, Qiagen, San Diego, CA, USA), quantified by spectrophotometry and assessed for quality using the Agilent 2100 bioanalyzer (Agilent Technologies ...
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bioRxiv - Genomics 2019Quote: Isolation of total mRNA for RNA-seq library preparation: Total RNA was isolated from the lysate using miRNeasy Mini Kit (Qiagen #217004) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Proteins (as well as DNA and RNA) were then extracted from the lysate using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germany) following manufacturers instructions ...
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bioRxiv - Immunology 2020Quote: ... Proteins (as well as DNA and RNA) were then extracted from the lysate using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germany) following manufacturers instructions ...
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bioRxiv - Bioengineering 2020Quote: ... and the same amount of lysate from each tumor was used to extract total RNA with the RNeasy Mini Kit (Qiagen, 74104) followed by reverse transcription using the same amount of template RNA ...