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Citations for Qiagen :
501 - 550 of 1720 citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... then clarified lysates were loaded onto a Ni2+ affinity column (Ni-NTA Superflow; Qiagen), washed in buffer A plus 300 mM NaCl and 20 mM imidazole pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... then clarified lysates were loaded onto a Ni2+ affinity column (Ni-NTA Superflow; Qiagen), washed in buffer A plus 300 mM NaCl and 20 mM imidazole pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were passed over 5 ml of packed Ni-NTA agarose resin (Qiagen) at 1.0 ml/min ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysate was added to a column with Ni-NTA agarose (QIAGEN, Hilden, Germany) and then washed with cold lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... and the clarified lysate was incubated in batch with Ni-NTA agarose resin (Qiagen) for 1 hour at 4°C with rotation ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysate was added to 1 mL of washed Ni-NTA bead slurry (Qiagen) and incubated for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were subjected to DNA extraction using the AllPrep DNA/RNA Mini Kit (Qiagen) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... and RNA was extracted from the lysates using an RNeasy® Mini kit (Qiagen). Purified DNA-free RNA was quantified ...
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bioRxiv - Microbiology 2020Quote: ... Lysate was applied to a prepacked gravity column of Ni-NTA agarose beads (Qiagen) equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... The gDNA was purified from the lysate using a QIAamp DNA mini kit (Qiagen). Sequence libraries of the V1 and V3 region of the 16S rRNA genes from each of the samples were constructed in accordance with the Dual Barcoded Two-Step PCR procedure from Illumina (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was loaded on 100 µl of Ni-NTA agarose beads (QIAGEN), pre-washed twice with 1 ml of denaturing washing buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was purified from cleared lysate using a QIAamp DNA mini Kit (Qiagen). For targeted NGS ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was gently shaken with 0.5 ml of Ni-NTA agarose suspension (Qiagen) for 1 h at 4 °C and transferred to a filter column where the liquid was drained ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Uncleaved protein with 6xHisTag were isolated from the lysate using Ni-NTA resin (Qiagen). The flow-through (FT ...
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bioRxiv - Cell Biology 2022Quote: ... Ear skin lysates were prepared by homogenizing the tissue with Homogenizer TissueRuptor II (QIAGEN) in TBS + 1% Triton-X100 + Protease and Phosphatase Inhibitor Cocktail (Sigma) ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant of the cell lysate was loaded onto Ni-NTA agarose resin (Qiagen) in Poly-prep chromatography columns (BioRad) ...
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bioRxiv - Genomics 2020Quote: ... Total RNA was then extracted from cell lysates using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNAs were extracted from homogenized cell lysate using RNEasy Plus mini kit (Qiagen), and the quality of extracted total RNA were examined using Nanodrop spectrum analysis and agarose gel electrophoresis ...
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bioRxiv - Developmental Biology 2019Quote: ... Frozen tissues were crushed with a pestle and the tissue lysates homogenized (QIAshredder, QIAGEN). Total RNA was isolated (RNeasy Mini Kit ...
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bioRxiv - Immunology 2020Quote: ... Residual DNA was removed from the cleared lysate using DNase digestion on columns (Qiagen). mRNAs were eluted in 40μL of RNase-Free water ...
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bioRxiv - Genetics 2020Quote: ... Cell lysates were incubated with 100 μl of Ni-NTA superflow beads (Qiagen, 30430) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was extracted from total cell lysate and cytosolic fractions using DNeasy kit (Qiagen). DNA samples were each diluted 1:10 and corresponding primers (Supplementary Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... was added to each dish and the lysate was collected into Qiashredder tubes (Qiagen) and centrifuged at 12,000 x g for 30 seconds for homogenization ...
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bioRxiv - Immunology 2021Quote: ... The clear solution containing lysate was passed through the RNeasy mini column (Qiagen, Germany), which leads to the binding of RNA with the column ...
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bioRxiv - Biochemistry 2020Quote: ... then clarified lysates were loaded onto a Ni2+ affinity column (Ni-NTA Superflow; Qiagen), washed in buffer A plus 300 mM NaCl and 20 mM imidazole pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were equalized for protein concentration and incubated with NiNTA agarose beads (Qiagen, #L30210) for O/N ...
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bioRxiv - Biochemistry 2021Quote: ... precleared lysates were mixed with 1 ml Ni-NTA-agarose beads (Qiagen; Hilden, Germany) and incubated for 1 h rotating at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... cell lysates were harvested using the RNeasy Mini kit (Qiagen #74104, Germantown, MD, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were processed for total RNA extraction using the RNeasy Micro Kit (Qiagen) and treatment with RNase-free DNase using the Ambion™ DNase I protocol (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from cell lysates and purified with RNeasy Mini Kit (Qiagen) or Direct-zolTM RNA MiniPrep Kit (Zymo Research) ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were loaded onto a gravity-flow column containing Ni–NTA resin (Qiagen) equilibrated in lysis buffer (without PMSF) ...
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bioRxiv - Genetics 2023Quote: ... Clarified lysate was applied to pre-equilibrated bed of Ni-NTA Agarose beads (Qiagen), washed extensively with wash buffer (15 mM Sodium Phosphate pH 7.6 ...
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bioRxiv - Cell Biology 2023Quote: ... The soluble fraction of the lysate was incubated with Ni-NTA agarose resin (Qiagen) on a rocking platform for 1 hr at 4°C ...
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bioRxiv - Genomics 2023Quote: ... DNA was then extracted from the lysate using the QIAamp DNA Mini Kit (Qiagen) with a modified protocol as follows ...
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bioRxiv - Systems Biology 2023Quote: ... total RNA was extracted from cell lysate by RNeasy Plus Mini Kit (Qiagen, 74136). Total RNA which was carried out following the method described above was removed rRNA by rRNA Depletion Kit (Vazyme ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The clarified lysate was incubated with nickel-nitrilotriacetic acid (Ni-NTA) agarose resin (Qiagen) for 1 hour with agitation ...
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bioRxiv - Synthetic Biology 2023Quote: ... the clarified lysate was loaded on a Ni-nitrilotriacetic acid (NTA) agarose column (Qiagen) using the ÄKTA Explorer fast protein liquid chromatography system (FPLC ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was extracted from thawed cell lysates using the RNeasy Plus Micro Kit (QIAGEN) following the manufacturer’s instructions and genomic DNA depleted using the supplied gDNA eliminator columns ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin for 90 minutes stirring in a beaker at 4ºC ...
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bioRxiv - Microbiology 2023Quote: ... and the filtered lysate was then loaded onto a Ni-NTA spin column (Qiagen). The column was washed five times with buffer A containing 10 mM imidazole ...
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bioRxiv - Genomics 2024Quote: ... total RNA was isolated from cell lysates using AllPrep DNA/RNA Mini Kit (QIAGEN). RNA quality was assessed based on RNA integrity number (RIN ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysates were treated with 4 μl of Qiagen protease (QIAGEN, Hilden, Germany, #1017782,) at 56°C for 10 min ...
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bioRxiv - Genomics 2024Quote: RNA from cell lysates was extracted using the Qiagen RNeasy mini kit (Qiagen 74106). Cell lysates were extracted in a randomized order to prevent batch effects in downstream analysis ...
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bioRxiv - Microbiology 2019Quote: ... viral RNA was extracted from the egg-isolate of the virus from the nasal swab using the QIAmp viral RNA mini-kit without the addition of carrier RNA (Qiagen, Manchester, UK). cDNA was synthesized from RNA using a random hexamer primer mix and cDNA Synthesis System (Roche ...
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Host transcriptomic profiling of COVID-19 patients with mild, moderate, and severe clinical outcomesbioRxiv - Genomics 2020Quote: RNA was extracted from nasopharyngeal swabs using the QIAamp Viral RNA Mini or the 213 EZ1 DSP Virus Kits (Qiagen, Hilden, Germany). All patients in this study tested positive for SARS-CoV-2 by RT-qPCR performed at Dubai Health Authority Hospitals ...
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bioRxiv - Molecular Biology 2020Quote: ... The entire 200-µL reaction was used as input for ssAAV gDNA extraction using a MinElute Virus Spin Kit (Qiagen Cat#57704). 50-ng of ssDNA from each sample were bisulfite converted using the EZ Methylation Gold kit (Zymo Cat#D5005 ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was extracted from virus library and ferret nasal wash samples using the QIAamp® Viral RNA mini kit (Qiagen, Germany). Next generation sequencing was carried out twice on the nasal washes ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA was purified using the QIAamp 96 virus QIAcube HT Kit and processed on the QIAcube robotic extraction platform (QIAgen, Hilden, Germany)
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bioRxiv - Microbiology 2022Quote: ... A wild-type virus stock NL4-3 was prepared by transfection of the pNL4-3 plasmid (purified using the Qiagen Plasmid Maxikit) into HeLa cells ...
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bioRxiv - Immunology 2019Quote: ... RNA was isolated from plasma samples using the QIAsymphony Virus/Bacteria Midi kit on the QIAsymphony SP automated sample preparation platform (Qiagen, Hilden, Germany). RNA was extracted manually if plasma volumes were limited ...