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Citations for Qiagen :
551 - 600 of 1973 citations for 4 Methoxy 6 methyl 6 phenyl 5H pyran 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and DNase I (2 mg (Qiagen) were dissolved in 30 mL of
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bioRxiv - Microbiology 2024Quote: ... 2 µg of RNase A (Qiagen) were added to each sample ...
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bioRxiv - Zoology 2024Quote: ... 2 µl 5x Q-Solution (QIAGEN), 1 µl primer mix (2 µM each primer) ...
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bioRxiv - Immunology 2020Quote: ... Samples were diluted at 4:1 (elution buffer (Qiagen)/cDNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Samples were treated with 4 μL Rnase A (Qiagen) to eliminate RNA contamination ...
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bioRxiv - Immunology 2021Quote: ... ears are collected on day 4 in RNAlater (Qiagen) and stored at 4° C until processed ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 µl of 5X PCR buffer (Qiagen; Hilden, Germany), 0.8 µl of 10 mg/ml BSA (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... was lysed using 4% SDS and a qiashredder (Qiagen) and analyzed by Western blot.
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bioRxiv - Neuroscience 2023Quote: ... into 4 μl Buffer TCL (1,031,576; Qiagen, Venlo, Netherlands) plus 1% 2-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... or a DNeasy UltraClean Microbial Kit (Qiagen 10196-4). 16S rRNA gene amplicons were generated using Earth Microbiome Project-recommended 515F.806R primer pairs using the 5PRIME HotMasterMix (Quantabio 2200410 ...
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bioRxiv - Microbiology 2024Quote: ... 365 µL of PowerBead solution (Qiagen, #12955-4-bs) and 20 µL of 20% sodium dodecyl sulfate (SDS ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in growth medium for one hour before lysis and RNA purification with the RNeasy Mini Plus Kit (Qiagen). Control cells were treated identically except for the five-minute addition of dissociating enzyme.
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bioRxiv - Genomics 2020Quote: ... DNA material obtained from one footprinting reaction (1µg) was bisulfite converted following standard Epitect bisulfite conversion kit protocol (Qiagen 59124). Entire material of one conversion was equally distributed to a 96-well plate and amplified via PCR using KAPA HiFi Uracil+ Kit (Roche ...
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bioRxiv - Immunology 2021Quote: ... PRRSV-2 RNA was used to amplify cDNA encoding the full-length of N gene by RT-PCR (One-step RT-PCR, QIAGEN) using specific primers with the forward containing a T7 promoter sequence at the 5’ end ...
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bioRxiv - Physiology 2020Quote: ... ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen) on an iCycler instrument (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR E assay12 using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: Genomic DNA from 1×106 FACS sorted p7 MPs from one animal was isolated with the All Prep DNA / RNA / Protein Mini Kit (Qiagen) and used as one sample ...
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bioRxiv - Microbiology 2020Quote: ... DNA from the same PER094-GFP line and the same batch as the one used for single cells was extracted with the QIAamp DNA Mini Kit (Qiagen) according to manufacturer’s instructions and used as bulk control (further called PER094 control).
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bioRxiv - Microbiology 2021Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR assay (24, 25) using the QuantiFast probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... Genomic DNA was extracted from one half of the hypothalamus using the DNeasy Blood & Tissue Kit (Qiagen, Germantown, MD, USA) and concentrated using the Genomic DNA Clean & Concentrator Kit (Zymo Research ...
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bioRxiv - Developmental Biology 2022Quote: Whole organoids were washed once with 1X PBS and placed into 2ml microcentrifuge tubes containing one 5mm steel bead (Qiagen) and 1ml of Trizol reagent (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... One μg of total RNA was retro-transcribed into complementary DNA (cDNA) by using the QuantiTect Reverse Transcription Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen) on an iCycler instrument (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... we used both one-step RT-PCR and two-step (cDNA synthesis followed by separate PCR amplification) reaction designs with commercial One-Step Ahead RT-PCR Kit (Qiagen) or IEH in-house RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... reverse transcription and amplification of the S gene were done with the one-step QuantiFast Sybr Green RT-PCR mix (Qiagen) as follows ...
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bioRxiv - Microbiology 2020Quote: ... The SVIP-MPv2 assay was developed and validated as a one-step procedure using the QuantiNova Probe RT-PCR Kit (Qiagen) with final concentrations of primers (1.4μM each ...
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bioRxiv - Bioengineering 2021Quote: ... Total RNA (5 μl) was used for cDNA synthesis and qPCR was performed in one step using QuantiTect Probe RT-PCR (Qiagen) on a StepOnePlus System (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... RNA was transcribed using a T7 RNA polymerase reaction and subsequently DNAse-treated for one hour followed by purification using a RNeasy kit (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Gene expression was evaluated by qPCR using the ONE-step QuantiTect Probe RT-PCR Kit (Qiagen, Cat No./ID: 204445). For each reaction 2 μL of RNA were used ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR E assay33 using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Pathology 2021Quote: ... and from lung and/or heart tissues of seropositive rodents were used to amplify viral genome by RT- PCR using the One Step RT-PCR kit (QIAGEN) followed by nested or heminested PCRs (Taq Pegasus ...
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bioRxiv - Microbiology 2020Quote: ... EAV genome copy number was determined by one step RT-qPCR using the QuantiTect™ Virus + ROX Vial Kit (Qiagen). Each RNA sample was tested in triplicate ...
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bioRxiv - Cell Biology 2020Quote: ... The RNA concentration was measured using Geneflow Nanophotometer and RT-qPCR was performed with one-step QuantiFast SYBR Green qPCR kit (Qiagen) using 50ng of RNA in each sample ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... reverse transcription and amplification of the S gene were performed using the one-step QuantiFast Sybr Green RT-PCR mix (Qiagen) as follows ...
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bioRxiv - Genomics 2021Quote: ... RNA was extracted using a QIAGEN RNeasy Mini Kit followed by two rounds of DNase digestion: one on-column using the QIAGEN RNase-Free DNase Set (79254, QIAGEN); and one off-column using Invitrogen’s TURBO DNA-free Kit (AM1907 ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from one wild-type control and three patient cell lines using DNeasy Blood and Tissue kit (Qiagen). Specific primers were designed to cover approximately 500 base pairs around the mutations and the regions were PCR-amplified using Qiagen Multiplex PCR kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA from the original material used for next-generation sequencing of the serum and passage 3 cultured MRI103 virus was used as the template for amplification using the One-Step Ahead RT-PCR kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... Crystals of SB16-RBD and SB45-RBD complexes and Sb16 alone were observed within one week using Protein Complex (Qiagen) and Wizard Classic 4 (Rigaku) ...
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bioRxiv - Immunology 2021Quote: ... 5 μl RNA was used in a one-step real-time RT–PCR E assay using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... Five μl RNA was used in a one-step real-time RT-PCR using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... 5’-AGCGTCATCAGGATTGGCAA and probe 5’-TGGGTGTCTGCTTTGGAACA were used in a one-step qRT-PCR reaction with either Quantifast reagents (Qiagen) for tissue RNA or LightCycler 480 RNA Master Hydrolysis Probes (Roche ...
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bioRxiv - Immunology 2020Quote: ... and a volume that equated to 17.5 mg of tissue was extracted using the BioSprint™96 One-For-All vet kit (Qiagen) and Kingfisher Flex platform as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at low speed (~1000 RPM) and lysed with one mL RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen).
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bioRxiv - Microbiology 2022Quote: ... The eluted RNA from tissues and swabs was analyzed by one-step qRT-PCR using 5 μL input RNA with the QuantiFast Probe PCR kit (Qiagen) and primer/probe sets targeting the NiV Malaysia and HeV N gene ...
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bioRxiv - Immunology 2022Quote: ... One volume of 70% ethanol was added and then transferred to a column from the RNeasy mini kit (Qiagen, 74104). RNA isolation was performed according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... One hundred microliters (uL) PBS was added to NW pellets followed by 280uL AVL lysis buffer (Qiagen, Germantown, MD, USA). Two to three mL blood was collected from the cranial vena cava in an SST tube for serum separation ...
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bioRxiv - Microbiology 2024Quote: ... corresponding to the three extraction protocols we used: (NM-1) one negative control for the kit DNeasy Blood and Tissue (Qiagen) used for mosquito DNA extraction ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were vortexed and frozen for at least one day at −80° before being thawed on ice and processed for RNA using an RNeasy Mini Kit (Qiagen). Purified mRNA was converted to cDNA with the High Capacity cDNA Reverse Transcription kit (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: Vector genomes present in each treated animal was quantified after preparation of genomic DNA from one brain hemisphere with the AllPrep96 DNA/RNA Kit (Qiagen). qPCR with primers and a probe directed against the hSyn promoter sequence (forward 5’-ATGAGTGCAAGTGGGTTTTAGGA-3’ ...