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Citations for TriLink BioTechnologies :
451 - 500 of 533 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Cell lysates were prepared and 12 fractions were collected from polysomes fractionated using the protocol for riboseq gradient preparation above (without nuclease treatment.) 0.3 ml of each gradient fraction was spiked with equal amounts of control RNA (Fluc mRNA, Trilink Biotechnologies, USA), then total RNA was extracted using the hot acid phenol-chloroform method (34) ...
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bioRxiv - Immunology 2021Quote: ... Cas9 mRNA (Trilink, 60ng/μl), Cas9 protein (PNABio ...
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bioRxiv - Immunology 2021Quote: ... Cas9 mRNA (Trilink, 60ng/μl), Cas9 protein (PNABio ...
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bioRxiv - Immunology 2021Quote: RNA was synthesized by Trilink Biotechnologies as a modified mRNA transcript with full substitution of 5-Methoxy-U ...
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bioRxiv - Immunology 2021Quote: ... eGFP-encoding mRNA (TriLink) was delivered at 200 μg mL-1(50 μg mRNA ...
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bioRxiv - Immunology 2021Quote: ... CVnCoV contains a cleanCap (Trilink), parts of the 3’ UTR of the Homo sapiens alpha haemoglobin gene as 3’ UTR ...
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bioRxiv - Cell Biology 2021Quote: ... CleanCap® mCherry mRNA (TriLink Biotechnologies), and guide RNAs for GFP and scrambled guides were used as a controls ...
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bioRxiv - Genomics 2021Quote: ... was used according to the company’s recommended instruction but with full substitution of N1-methyl-pseudouridine for uridine and co-transcriptional capping with CleanCap AG (TriLink Biotechnologies). RNA was precipitated with lithium chloride (2.5 M final concentration) ...
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bioRxiv - Genomics 2021Quote: ... Restriction fragment ends were labeled with biotinylated cytosine nucleotides by biotin-14-dCTP (TriLINK). Blunt-end ligation was carried out at 16°C overnight in the presence of 50 Weiss units of T4 DNA ligase ...
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bioRxiv - Cell Biology 2021Quote: ... and/or Clean Cap®Cyanine5 (Cy5) Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) (TriLink Biotechnologies, L-7701).
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bioRxiv - Cell Biology 2021Quote: ... 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N [methoxy (polyethyleneglycol)-2000] (DMPE-PEG2000, NOF Corporation) and contained CleanCap® Enhanced Green Fluorescent Protein (eGFP) mRNA (5-methoxyuridine) (TriLink Biotechnologies, L-7201) and/or Clean Cap®Cyanine5 (Cy5 ...
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bioRxiv - Biophysics 2021Quote: ... Germany) and 3’-dATP was purchased from TriLink Biotechnologies (San Diego ...
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bioRxiv - Microbiology 2020Quote: ... rRNA depleted samples were prepared for sequencing using the Cleantag Small RNA library prep kit (Trilink, L-3206-24) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Chemically modified mRNA encoding ETV2 (modRNA(ETV2)) was generated by TriLink BioTechnologies ...
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bioRxiv - Cell Biology 2020Quote: ... ModRNA encoding GFP (TriLink, Cat No. L-7601) at a concentration of 0.2 μg per million h-MPCs served as negative control.
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bioRxiv - Cell Biology 2020Quote: ... T cells were electroporated with 15 ug Cas9 mRNA (TriLink) and 10 ug AAVS1 specific sgRNA using the Neon electroporator (3×105 in 10ul Neon tip ...
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bioRxiv - Molecular Biology 2020Quote: ... 2’-fluoro-dNTPs were from TriLink BioTechnologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared using the TriLink CleanTag Small RNA Ligation kit (TriLink Biotechnologies, San Diego, CA). Seven to eight libraries were sequenced per lane with single-end 50x on the HiSeq2500 platform ...
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bioRxiv - Molecular Biology 2020Quote: ... The following primers were then used to amplify respective cut sites at HBA2 and HBA1 along with CleanAmp PCR 2x Master Mix (TriLink, San Diego, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The library was synthesized by Trilink Biotechnologies ...
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bioRxiv - Genomics 2020Quote: ... The library was synthesized by Trilink Biotechnologies ...
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bioRxiv - Genomics 2020Quote: ... The NEB HiScribe High-Yield Kit was used as per the instruction manual but with full substitution of N1-methyl-pseudouridine for uridine and co-transcriptional capping with CleanCap AG (Trilink). Reaction cleanup was performed by lithium chloride precipitation ...
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bioRxiv - Genomics 2020Quote: ... A doped oligo library of 168 bases was ordered (TriLink Biotechnologies) containing 30 bases of invariant flanking sequence on either side of the 108 bases of the doped target region (2.5% base misincorporation) ...
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bioRxiv - Genomics 2020Quote: ... and bio-16-CTP (Trilink) and bio-16-UTP (Roche ...
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Neutralizing PD-L1 and PD-L2 Enhances the Efficacy of Immune Checkpoint Inhibitors in Ovarian CancerbioRxiv - Cancer Biology 2020Quote: ... and the nucleotide analogs 8-oxo-dGTP and dPTP (TriLink Biotech). Six separate PCR reactions were performed in which the concentration of analogs and the number of cycles were varied to obtain a range of mutation frequencies ...
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bioRxiv - Genetics 2020Quote: ... 2E5 cells were transfected with 500ng of GFP mRNA (TriLink Biotechnologies, cat# L-7201) or RNP using a 96-well Shuttle nucleofector (Lonza ...
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bioRxiv - Biochemistry 2020Quote: ... SH-SY5Y cells were electroporated or chemical transfected with commercially available non-labeled (NL) or Cyanine5-labeled (Cy5) eGFP encoding mRNAs (Trilink®) has described here.
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bioRxiv - Bioengineering 2020Quote: ... R-PE antibody conjugation kit was obtained from TriLink Biotechnologies ...
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bioRxiv - Bioengineering 2020Quote: ... 5′-Biotin-G-Monophosphate (TriLink, #N-6003) at a 20 mM (final concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... All modified NTPs were purchased from TriLink. The sequences included in the short unmodified dataset (UNM-S) ...
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bioRxiv - Genetics 2020Quote: ... along with Cas9 mRNA (Trilink) at 100 ng/μl and Cas9 protein (PNABio ...
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bioRxiv - Molecular Biology 2020Quote: ... each parental line was transfected with 1 µL of 100 µM sgRNA (Synthego Corporation) and 1 µg Cas9 mRNA (TriLink #L-7206) using the Neon Transfection System following standard protocols ...
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bioRxiv - Biochemistry 2020Quote: ... PNTs were purchased from TriLink BioTechnologies
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bioRxiv - Bioengineering 2020Quote: ... 1 ng was used as template for initial error-prone PCR using 8-oxo-GTP and dPTP (TriLink Biotechnologies). Four 50 µl PCR reactions were performed on each template with increasing concentrations of mutagenic nucleotides (5 µM ...
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bioRxiv - Bioengineering 2020Quote: Fluc mRNA and hsACE2 variant mRNA were purchased from TriLink Biotechnologies (CA ...
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bioRxiv - Bioengineering 2020Quote: ... 2’-Fluoro-2’-deoxycytidine-5’-Triphosphate (2’F-dCTP) and 2’-Fluoro-2’-deoxyuridine-5’-Triphosphate (2’F-dUTP) were purchased from Trilink Biotechnologies ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.5 μg CleanCap Cas9 mRNA (TriLink Biotechnologies, San Diego, CA) and 1 μg of modified gRNA were added to 1×106 cells in 20 μL of the recommended electroporation buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... The sgRNA (50ng/uL) and Cas9 mRNA (25ng/uL, TriLink) was microinjected into the pronuclei of FVB/NJ zygotes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Nucleoside triphosphates and nucleoside triphosphate analogues were purchased from TriLink BioTechnologies (Biotin-16-dUTP ...
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bioRxiv - Microbiology 2020Quote: ... Virus was plaque-purified on Vero CCL81 cells in the presence of 25 μg/ml of cytosine arabinoside (TriLink BioTechnologies), and plaques in agarose plugs were amplified on Vero CCL81 cells ...
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bioRxiv - Biophysics 2020Quote: ... except each nucleotide was present at a final concentration of 4 mM and the reaction was supplemented with 6 mM of 5’-biotin-G-Monophosphate (Trilink, #N-6003). Reactions were incubated at 37 °C for 2 hours ...
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bioRxiv - Biochemistry 2020Quote: ... and 2’-Azido-2’-dATP (TriLink BioTechnologies, San Diego, California) in 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... The standards Am and m6Am 5’ monophosphate were generated by digesting their triphosphate forms (TriLink N-1015 and N-1112) with 1 unit Apyrase (NEB M0398).
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bioRxiv - Neuroscience 2020Quote: ... Commercial Cas9 mRNA was ordered from TriLink (L-6125, San Diego, CA) and used for the embryo microinjection ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 mM ApA RNA dinucleotide (5’ to 3’; TriLink BioTechnologies) was suspended in degassed 20 mM HEPES/NaOH pH 7.6 ...
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bioRxiv - Microbiology 2020Quote: ... 2’-F-2’-dUTP (N-1010-1) and didanosine-TP (N-4017-1) were purchased from Trilink. Remdesivir-DP was synthesized by WuXi AppTech ...
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bioRxiv - Immunology 2020Quote: ... we first diluted 0.42 μL of the GFP mRNA (Trilink, stored at 1 μg/μl) into 7.26 μL of PBS (pH 5.5 ...
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bioRxiv - Immunology 2020Quote: ... Uridine 5’-triphosphate (UTP) was replaced with the triphosphate derivative of m1Ψ (m1ΨTP) (TriLink, San Diego, CA, USA Biotechnologies) in the transcription reaction ...
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bioRxiv - Immunology 2020Quote: ... cells were electroporated in the presence of Cas9 mRNA (TriLink) and CD56 guide RNA (CGCU-GAUCUCCCCCUGGCU ...
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bioRxiv - Immunology 2020Quote: ... Capping of the IVT mRNAs was performed co-transcriptionally using the trinucleotide cap1 analog CleanCap (TriLink, San Diego, CA, USA). After DNase digestion ...