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Citations for abcam :
401 - 450 of 542 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... the chromatin fraction was solubilized in EBC-150 buffer with 500 U/mL Benzonase and 2 ug of antibody directed to endogenous RNAPII-S2 (Abcam; ab5095) or RNAPII-S5 (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... Resin was washed 4X in 800 uL EMB 300 Buffer and then protein association was analyzed by Western blot using antibody for hnRNP A2/B1 (Abcam #ab6102). Additionally ...
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bioRxiv - Microbiology 2021Quote: ... then incubated 1 hour at room temperature in blocking buffer containing a 1:10,000 dilution of goat anti-Rabbit IgG H+L HRP-conjugated (Abcam Cat #ab63797). Blots were washed again in 3X TBS-T then incubated in blocking buffer containing a 1:5000 dilution of rabbit Anti-DDDDK HRP conjugated antibody (Abcam Cat #ab2493) ...
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bioRxiv - Neuroscience 2019Quote: ... Followed by incubation with goat anti-rabbit lgG H&L (Alexa Fluor® 594) preadsorbed secondary antibody (1:500 diluted in antibody dilution buffer, Cat #: ab150088, Abcam) for 2 hrs at room temperature in dark ...
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bioRxiv - Cancer Biology 2019Quote: ... the first wash with wash buffer A after ON hybridization contained 1:800 diluted anti-mouse secondary antibody labeled with Alexa Fluor 488 (Abcam, ab150113).
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bioRxiv - Microbiology 2019Quote: Immunostaining for p62 and ubiquitin was performed with blocking and permeabilization buffer (1% saponin, 3% bovine serum albumin) and anti-p62 rabbit monoclonal antibodies (Abcam #AB109012) at a concentration of 1 μg/ml or anti-ubiquitylated protein antibodies (clone FK2 ...
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bioRxiv - Physiology 2021Quote: ... If not otherwise mentioned heat induced antigen retrieval with citrate buffer (pH 6) was performed prior to incubation with the following rabbit mono or poly-clonal antibodies: UCP1 (Abcam ab10983) 1:800 ...
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bioRxiv - Plant Biology 2019Quote: ... The membrane was blocked for 1 hour using 7.5% BSA in PBST (PBS containing 0.1% Tween20) buffer and incubated overnight at 4°C with the primary GFP antibody as required (1:2000 dilution, Abcam, ab290). After extensive washing ...
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bioRxiv - Molecular Biology 2022Quote: ... mitochondria were immunostained in 100 μL of Intercept Blocking Buffer containing 1 μL of Alexa Fluor 647 Anti-TOM20 antibody (Abcam, ab209606) for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: Beads that were incubated with FLI 7.3 mouse antibody were resuspended in 100 µL Dig-wash buffer and incubated with rabbit anti-mouse secondary antibody (Abcam, ab46540) at a dilution of 1:100 on a rotator for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were diluted in block buffer (1:50) and incubated for 45 minutes at room temperature (goat anti-guinea pig IgG H&L Cy3 (Abcam #102370), and donkey anti-mouse IgG H&L Alexa Fluor 647 (Abcam #150107) ...
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bioRxiv - Cancer Biology 2020Quote: ... Wash buffer was removed by placing the samples on a magnet and antibody buffer containing 0.0125% of digitonin and GATA1 antibody (Abcam, 1:100) or rabbit gG control antibody (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were blocked for 1 hour at room temperature with 5% fat-free milk or milk-free blocking buffer (for analysis of phosphorylated proteins) and incubated overnight at 4°C with primary antibodies for p-GCN2 (Abcam, ab75836), p-eIF2α (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... Then slides was washed with washing buffer (0.025% Tween-20/1X PBS) three times and the secondary antibody conjugated with Alexa 555 (ab150086, abcam, 1:1000) was applied ...
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bioRxiv - Microbiology 2022Quote: ... low or no ACE2 were resuspended in FACS buffer (PBS+2% BSA) and incubated for 1 h with 1:500 rabbit anti-ACE2 antibody (Abcam ab272500). Cells were washed with FACS buffer and labeled for 1 h with 1:3000 Alexa-Fluor-488 goat anti-rabbit IgG H&L (Abcam ab150077) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with FACS buffer and labeled for 1 h with 1:3000 Alexa-Fluor-488 goat anti-rabbit IgG H&L (Abcam ab150077). Cells were washed with FACS buffer and fixed with 4% PFA ...
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bioRxiv - Developmental Biology 2022Quote: ... for 1 hr at RT then stained O/N at 4° C using the following primary antibodies diluted in blocking buffer: Chromogranin A (1:200, Abcam, ab15160), Glucagon (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... the section was blocked with blocking buffer (Zymed Laboratories Inc., San Diego, USA) before incubated with anti-Citrobacter antibody (Abcam, ab37056) overnight at 4°C as previously described[33] ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then washed with TBST buffer (5 × 1 min) and probed with secondary rabbit anti-rat antibodies conjugated with peroxidase (1:1000, Abcam #ab6734). Blots were washed as described above and detection was carried out using Amersham ECL Western Blotting Select Detection Reagent (GE Lifesciences #RPN2235) ...
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bioRxiv - Developmental Biology 2022Quote: ... blocked for 1 h in blocking buffer (PBST with 10% donkey serum) and incubated with the primary antibody chicken anti-GFP (1:250 in blocking buffer) (Abcam, ab13970) overnight at 4 ºC ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with the indicated primary antibodies diluted in blocking buffer at 4°C overnight: mouse-pH3 (1:2000, Abcam, ab14955), rabbit-Ki67 (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... lysed with 0.2% Triton X buffer, blocked with 1% BSA, and incubated with primary antibodies (anti-RICTOR, anti-MAPKAP1, anti-GSN, anti-β-tubulin (Abcam), anti-mTOR ...
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bioRxiv - Biochemistry 2019Quote: ... the pellet was frozen at −20 °C and resuspended in 20 ml of lysis buffer (20 mM Tris, pH 7.6, 0.1 mM ZnCl2, supplemented with Protease Inhibitor Cocktail (EDTA-free; ABCAM, diluted 1:1000), Benzonase nuclease (MERCK ...
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bioRxiv - Cancer Biology 2020Quote: Mammary glands were dissected and homogenized in NOS assay buffer and further processed following the Nitric Oxide Synthase Activity Assay kit (Abcam, ab211083) protocol for measuring enzymatic activity of nitric oxide synthase (NOS) ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked in Licor blocking buffer and probed with the following primary antibodies for histone H3 (1:1000; Abcam #ab1791), H3K9me2 (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated overnight at 4°C in wash buffer containing Anti-CDKN2A/p16INK4a conjugated (Alexa Fluor 647) antibody (Abcam ab192054). Cells were then washed with PBS containing 1 µg/mL DAPI for five minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... We used extraction buffer and washed several times with PBS before immunofluorescence for ECM using anti-fibronectin-FITC (1:50 dilution; Abcam, ab72686). Samples were imaged using a Zeiss LSM 780 microscope using either a 20x 0.75NA objective or 10x 0.45NA objective.
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bioRxiv - Neuroscience 2020Quote: ... the blot was incubated with 1:1000 diluted buffer of secondary rabbit anti-mouse HRP conjugated antibody (Cat # 6728, Abcam, UK) in 1X PBS for 1 h at room temperature ...
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bioRxiv - Genomics 2021Quote: ... The cells were resuspended by gently flicking the tube in the same blocking buffer containing 1:250 dilution of rabbit anti lamin-B1 antibody (Abcam, #ab16048) and incubated overnight at 4 °C in a microfuge tube rack ...
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bioRxiv - Immunology 2021Quote: ... Grids were permeabilized and blocked with 0.1% Saponin/1% cold-water fish skin gelatin (Perm buffer) for 10 min and first stained with anti-human CD63 (Abcam, ab59479) antibody (1:10 ...
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bioRxiv - Bioengineering 2021Quote: ... Primary antibody solutions were prepared by diluting one or more of the following antibodies in blocking buffer: mouse anti-HNF4a (1/200 from stock, Abcam ab41898), goat anti-OPN (1/60 from stock ...
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bioRxiv - Bioengineering 2021Quote: ... Secondary antibody solutions were prepared by diluting one or more of the following secondary antibodies in blocking buffer: DyLight 550-conjugated donkey anti-mouse IgG (1/50 from stock, Abcam, ab98767) and DyLight 488-conjugated donkey anti-goat IgG (1/50 from stock ...
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bioRxiv - Immunology 2021Quote: ... Coverslips were incubated overnight at 4 °C in blocking buffer and probed the following morning with 1 :500 rabbit anti-ACE2 (Abcam ab15348) for 1h at room temperature on an orbital rocker ...
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bioRxiv - Developmental Biology 2020Quote: ... Then the membranes were incubated with following primary antibodies at appropriate dilution in blocking buffer: ant-SATB1 (ab109122, Abcam 1: 10000), anti-SATB2 (ab92446 ...
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bioRxiv - Genomics 2021Quote: ... Fragmented DNA was then diluted 10 times with ChIP dilution buffer and protein-DNA complexes were captured using an H3K27ac antibody (Abcam AB4729). Next ...
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bioRxiv - Immunology 2022Quote: ... cells were washed with 1 ml of FACS buffer and resuspended in 500 µl of goat anti-rabbit Alexa Fluor 488 antibody (Abcam, ab150077) at 0.67 µg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 100 μl cold Antibody Buffer and 1□0μl antibody (H3K4me3, EpiCypher #13-0041; H3K27Ac, Active Motif #39133; SOX11, Sigma #HPA000536 or HNRNPH1 Abcam #ab154894) was added for incubating on nutator overnight at 4□°C ...
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bioRxiv - Developmental Biology 2022Quote: ... protein was extracted from the 8 cultures using the lysis buffer from the GFP Fluorescent ELISA Kit (ab229403) (Abcam, Cambridge, UK) that was supplemented with Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were rinsed in washing buffer and incubated with DAPI for 20s before being coverslipped with Fluoroshield mounting medium (Abcam, ab104135).
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bioRxiv - Cell Biology 2022Quote: ... the membrane was blocked using the Odyssey blocking buffer (LiCor) and incubated overnight at 4 °C with the primary antibody (SHIP2 antibody (Abcam #ab70267) 1/2000 ...
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bioRxiv - Microbiology 2023Quote: ... 5 s at 60% amplitude) in 320 µL of buffer A containing 2 % (v/v) NP-40 (Abcam, Cat. No. ab142227). The aggregated proteins were sedimented at 15,000 x g for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Grids were washed in blocking buffer 5x 2 mins and then incubated in 10 nm colloidal gold-conjugated anti-rabbit secondary antibody (Abcam, ab27234) diluted 1:20 in blocking buffer for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Co-IPs were performed by using the magnetic Sure Beads Protein A from BioRad washed with dilution buffer (10mM Tris, 150mM NaCl, 0.5mM EDTA PH=7.4, and coated with the Talin antibody (Abcam ab71333 5µg/200mL). The protein lysates were added to the beads for 1h at room temperature before being washed with washing buffer (10mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated in 2x PBS with 0.2% Tween and blocking buffer (1:1) with primary antibodies rabbit anti-AUF1 (Abcam, 1:5000), rabbit anti-phospho-AUF1 (Abcam ...
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bioRxiv - Biophysics 2023Quote: ... we added a 1:400 solution of primary antibody in blocking buffer (anti-GM130 ab52649 Abcam, anti-tubulin Millipore Sigma T6199). We incubated coverslips with the primary antibody overnight at 4 °C ...
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bioRxiv - Physiology 2023Quote: ... incubated with blocking buffer (PBS + 5%FCS +0,1%Tween) for 1 hour and stained with anti-Iba1 antibody (diluted 1:300, Abcam; Cat#ab178846) and /or anti-TOM20 antibody (diluted 1:1000 ...
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bioRxiv - Genetics 2023Quote: ... followed by a permeabilisation step using 0.3% Triton X-100 for 10 min and then treated with 1% BSA blocking buffer solution for 2 h prior to overnight incubation with monoclonal anti-pNRF2 (1:200; Abcam ab76026) in a humidified chamber at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): anti-CD146 (Abcam #ab75769); anti-#-SMA (Abcam #ab5694 ...
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bioRxiv - Physiology 2023Quote: ... The preparation was passed through a 70 μm filter and treated with Red Blood Cell lysis buffer (Abcam, Waltham, MA, USA). Single cells were resuspended in PBS containing 0.04% BSA ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated in a blocking buffer with 20 ng/μl of commercial secondary antibody goat anti-mouse IgG H&L (Alexa Fluor 647) (abcam, ab150115) for 45 min ...