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Citations for abcam :
251 - 300 of 542 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Slides were subsequently blocked with blocking buffer (3% natural serum, 1% BSA, 0.1% Tween-20) and incubated overnight with anti-EZH2 antibody (3748; Abcam), anti-CREB3L1 (AF4080 ...
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bioRxiv - Molecular Biology 2021Quote: ... 96-well MaxiSorp round bottom U96 Nunc plates were coated overnight at 4°C with 50 µl of 50 mM sodium phosphate (pH 7.5) and 10% glycerol buffer containing 0.5 µg of goat anti-GFP IgG (Abcam; Ab6673). Wells were washed once with 200 µl of TCS buffer prior to immunoprecipitation ...
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bioRxiv - Molecular Biology 2021Quote: ... Washed beads were resuspended in 400µl lysis buffer and 5µl anti-Piwi (Hannon Lab) or rabbit IgG antibodies (Abcam, ab37415) added ...
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bioRxiv - Cancer Biology 2019Quote: 3 × 106 cells were collected 48 hours after cell seeding and were lysed with 0.1 ml lactate assay buffer supplied in the L-Lactate assay kit (ab65330, Abcam) followed by centrifugation at 16,000 x g for 10 min ...
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bioRxiv - Genetics 2019Quote: ... Dynabeads M-280 sheep anti-rabbit IgG were incubated in lysis buffer and 0.5% BSA for 2 hrs with either rabbit anti-GFP (Abcam) for query IPs or 5 μl of rabbit-anti HA (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... Heat-induced antigen retrieval in citrate buffer (pH 6.0) was performed prior to incubation with primary antibodies: anti-histone H3 (Abcam); anti-Fibrinogen (Thermofisher)) ...
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bioRxiv - Bioengineering 2021Quote: ... which were incubated overnight at 4 °C with primary antibodies diluted 1:5,000 in blocking buffer (BioLegend Way, San Diego, CA, cat. #312102 and #353013; LSBio, Seattle, WA, cat #B6014; Abcam, Cambridge ...
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bioRxiv - Cancer Biology 2021Quote: ... lysates were diluted twofold in assay buffer and incubated with capture beads for FUT8 (protein G-agarose beads; Abcam) overnight ...
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bioRxiv - Cell Biology 2021Quote: ... cells cultured in 6-well plates were collected in 50µl of the Iron Assay Buffer provided in an Iron Assay kit (catalog no. ab83366, Abcam) and were homogenized with pellet pestles associated with a motor ...
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bioRxiv - Cell Biology 2021Quote: ... slides were stained with primary antibodies for CD3 (one drop of the prediluted antibody per 200μl staining buffer, clone SP7, Abcam) and HIV envelope (1:300 AG3 ...
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bioRxiv - Cell Biology 2021Quote: ... tissue sections were stained with primary antibodies for CD3 (one drop of the prediluted antibody per 200μl staining buffer, clone SP7, Abcam) and HIV envelope (1:300 AG3 ...
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bioRxiv - Neuroscience 2020Quote: ... resuspended in Buffer A with 0.1% BSA and then loaded with either anti-GFP polyclonal antibody (25 µg per IP, abcam) or anti-HA-tag monoclonal antibody (2.0 µg per IP ...
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bioRxiv - Cell Biology 2020Quote: ... Blocking buffer was aspirated and cells incubated for 1 h at RT with a 1:1,000 dilution of primary antibodies in 2% BSA blocking buffer against dsDNA (Abcam, Cambridge ...
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bioRxiv - Cancer Biology 2020Quote: ... Antigen retrieval used citrate buffer pH9.0 with 30’ incubation, followed by staining for ER (SP1, 1:100) or MDC1 (Abcam ab11171 ...
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bioRxiv - Biochemistry 2022Quote: ... co-immunoprecipitated proteins were eluted from the magnetic beads by incubating with 10 µL 4X LDS Sample Buffer (Abcam) at 70 °C for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... diluted in blocking buffer for one hour at RT: donkey anti-rat Alexa Fluor® 647 (1:250; abcam); donkey anti-goat Alexa Fluor® 568 (1 ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were incubated with appropriate mouse antibodies directed against TLR4 (ab22048, 1:1000 diluted in Odyssey buffer, abcam) e IκBα (Cat # ...
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bioRxiv - Microbiology 2020Quote: ... and 20 mM of L-lysine monohydrochloride in 50 mM Tris/HCl buffer (pH 7.4) were incubated in the presence or absence of ubiquitin-aldehyde (6 nM, Abcam) for 30 min at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... blocked in eBioscience Blocking Buffer (LifeTech Thermo 00-4953-54) followed by staining with anti-Lamp1 1D4B (Abcam ab25245). For fluorescent imaging ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then incubated in 10% H2O2 in PBS to reduce background and heated in a microwave in citrate buffer (pH 6.0; Abcam) for antigen retrieval ...
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bioRxiv - Neuroscience 2022Quote: ... previously washed with 5% BSA in TBS-Triton buffer and preincubated overnight with 2.5 μg anti-GFP antibody ab290 (Abcam). For the negative control ...
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bioRxiv - Immunology 2022Quote: ... cells were washed and incubated with a staining buffer containing Alexa Fluor 647 conjugated goat anti-mouse antibody (Abcam), Alexa fluor 488 conjugated goat anti-rabbit antibody (Abcam) ...
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bioRxiv - Neuroscience 2019Quote: ... After cooling, sections were washed with PBS and incubated in blocking buffer (0.5% Triton-X, 10% normal donkey serum (Abcam) in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... The beads were resuspended in 100 μL reaction buffer and incubated with 2.5 μg anti-m6A antibody (Abcam # ab151230) for 30 min at room temperature with constant rotation ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Sections were incubated on a shaker for 48 h at 4 °C with primary antibodies in the blocking buffer containing 0.1% Triton X-100 at the following dilutions: rabbit anti-TH (1:1000, Abcam), chicken anti TUJ1 (1:1000 ...
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PALP: An imaging method for detecting and quantifying polyunsaturated phospholipids via peroxidationbioRxiv - Cell Biology 2020Quote: ... Membranes were blocked with 50% Odyssey blocking buffer (LiCor) diluted with 0.1% Tween-20-containing Tris buffered saline (TBST) and immunoblotted with antibodies against ACSL4 (Abcam, ab155282 ...
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bioRxiv - Bioengineering 2019Quote: ... Plates were washed with washing buffer (PBS 0.1% Triton) and 200 µL/well Horseradish peroxidase conjugated-Infliximab (HRP conjugation Kit, abcam) (0.2 µg/mL IFX-HRP ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated for 2.5 hr with primary antibodies diluted in blocking buffer (IdU, B44, 347580; BD; CldU, ab6326, Abcam), washed several times in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... The 2 ml of whole blood were added to 40 ml of red blood cell lysis buffer (Abcam, ab204733) and incubated for 10 min at room temperature to lyse red blood cells ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were blocked and permeabilized in blocking buffer (10% [v/v] normal goat serum [NGS] (Abcam, Cambridge, United Kingdom) in PBS/Gly with 0.1% [v/v] Triton X100 ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then stained overnight in the same blocking buffer with primary antibodies against GFP (1:2000 Abcam 13970), YAP (1:200 Cell Signaling Technologies 14074S) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were prepared in blocking buffer (SHIP1, 1:100 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; ASC ...
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bioRxiv - Neuroscience 2022Quote: ... Dynabeads were then resuspended in 500uL washing buffer and the appropriate volume of antibody: APP C-terminal Y188 (3µg, ab32136, Abcam), PSD95 (3µg ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads were resuspended in 1 mL ChIP dilution buffer with 8 μg of anti-HA antibody (Abcam ab9110) and incubated on a rotator at 4°C for 6 h.
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bioRxiv - Microbiology 2023Quote: ... cells were washed with ice-cold PBS and soluble proteins were extracted with cell lysis buffer (100mM Tris-HCl pH=8.0, 150mM NaCl, 1% NP-40, phosphatase and protease inhibitor cocktail tablets (Abcam)) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were incubated with primary antibodies in blocking buffer overnight at 4°C using the following antibodies (all Abcam): anti-sarcomeric-alpha-actinin (ab9465 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice with PBS and lysed with ice-cold lysis buffer containing NP-40 (2%, ab142227, Abcam), Triton X-100 (2%) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary and secondary dilutions were made in blocking buffer consisting of 1:2000 rabbit anti-α-syntrophin (abcam, ab188873), 1:15,000 mouse anti-GAPDH (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed in wash buffer (PBS with 0.05% Tween) and incubated in blocking solution with rabbit anti-sheep IgG (Abcam) at 1:40,000 dilution for 1 hour at room temperature and visualised on LiCor Odyssey Fc (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with primary antibodies (ON, 4 °C, in blocking buffer) targeting Ki67 (abcam, ab16667, 1:250) and β-III-tubulin (abcam ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... incubation with the following primary antibodies was performed following routine heat antigen retrieval (10 mM sodium citrate buffer, pH 6.0): anti-cleaved PARP (Abcam, E51 ...
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bioRxiv - Physiology 2023Quote: ... with a ratio of 1 mg tissue to 5 µL cold TEM buffer (50 mM Tris, 1 mM EDTA, 0.5 mM MgCl2, pH 7.4) + 1x protease inhibitor cocktail (Abcam ab271306) with 30 up-and-down strokes on ice ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were then blocked in Odyssey blocking buffer for 1 hr at room temperature before incubation with the following primary antibodies in blocking buffer (Odyssey) and 0.1% Tween-20 at 4°C overnight: anti-DUSP6 (1:1,000, Abcam; ab76310); anti-BACE1 (1:1,000 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Supernatants were either assayed without dilution (most viral infections) or at an appropriate dilution in NS dilution buffer (Abcam).
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bioRxiv - Neuroscience 2023Quote: ... Deparaffinized and rehydrated slides were submerged in 1X antigen retrieval solution (100X Tris-EDTA Buffer, pH 9.0, ab93684, Abcam) and placed in a tissue steamer (IHC-Tek Epitope Retrieval Steamer Set ...
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bioRxiv - Bioengineering 2023Quote: ... blocking buffer was removed and the sections were incubated with primary anti-GFP antibody (Abcam, ab290, 1:200 dilution) overnight to ensure optimal binding ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were subjected to an antigen retrieval with 10 mM sodium citrate buffer (pH 6.0) and co-stained with hypoxyprobe 1 and DDX5 (ab126730, Abcam) antibodies was carried out ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were centrifuged and resuspended in calcium-rich binding buffer provided by the apoptosis staining kit (Abcam, #ab14085) to reach ~106 cells/mL ...
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bioRxiv - Biophysics 2022Quote: ... The blocking buffer was removed and cells were incubated with recombinant anti-Lamin B1 primary antibody (ab229025, by abcam) diluted 1:250 (volume/volume ...
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bioRxiv - Bioengineering 2023Quote: ... grids were washed 3x with blocking buffer + 0.2% Tween and floated on biotin-conjugated anti-rabbit secondary antibody (ab6720; Abcam) diluted 1:100 in blocking buffer + 0.1% Tween 20 ...