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Citations for abcam :
351 - 400 of 542 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The gels were run in MOPS buffer at 200 V for 45 minutes and stained with InstantBlue Coomassie protein stain (Abcam). Protein bands intensities were quantified using ImageJ76 and then plotted against fraction numbers using GraphPad Prism version 9.0.1.
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bioRxiv - Biochemistry 2022Quote: ... washed three times in blocking buffer and then incubated in 1:500 dilution of Alexa-488-conjugated goat anti-rabbit secondary (ab150077; Abcam) mixed with 1X rhodamine-phalloidin (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... The assays were carried out in 50 μl mTOR reaction buffer with or without 200 μM ATP and/or the recombinant human His-tagged ATF4 (Cat. ab109946, Abcam) at 30°C for 45 min ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... Membranes were then incubated with primary antibody diluted in TBS-T buffer (1:1000 Total OXPHOS Rodent WB Antibody Cocktail [abcam Cat# ab110413] or 1:1000 HSP60 XP Rabbit mAB [Cell Signaling Technology CAT#12165] ...
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bioRxiv - Immunology 2022Quote: ... Each well of a 6-well plate was incubated for 30min at room temperature with 200μL of Cell Extraction Buffer PTR (ab195215, ABCam, UK) containing phosphatase inhibitors and the protease inhibitor aprotinin ...
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bioRxiv - Immunology 2022Quote: ... we fluorescently labeled ∼300,000 CAR T cells at 37°C for 30 minutes in 1:1 (v/v) solution of live cell staining buffer (Abcam) and RPMI-1640 (Corning ...
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bioRxiv - Plant Biology 2022Quote: ... Sections were incubated with blocking buffer [3% (m/v) BSA in PBS buffer] for 1 h before overnight incubation with primary antibodies for H3K9me2 (1:400 dilution, abcam) and H3K27me1 (1:600 dilution ...
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bioRxiv - Genetics 2022Quote: ... the wells were blocked with Licor blocking buffer (927-40000, Licor, Cambridge, UK) for 90 minutes followed by incubation with Anti-ASL antibody (Abcam) for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... They were then blocked with rotation at room temperature for an hour in blocking buffer (5% normal goat serum (Abcam), 0.2% TritonX-100 PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight in the rabbit anti-Iba1 polyclonal antibody as a primary antibody (diluted 1:500 in washing buffer) (ab108539, Abcam). The next day ...
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bioRxiv - Neuroscience 2022Quote: ... The expression of ACE2 Receptor protein (Rabbit Anti-Human Polyclonal, Citrate Buffer HIER, dilution 1:2000, Abcam, Code Number: ab15348) and TMPRSS-2 protein (Rabbit Anti-Human Monoclonal ...
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed in blocking buffer (2x 10 min) and incubated with secondary antibodies: Alexa Fluor 488 goat anti-mouse (1:1000, Abcam), Alexa Fluor 594 goat anti-rabbit (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: Purified brain mitochondria were solubilized in Laemmli buffer and processed for Western blotting using standard procedures and Total OXPHOS Antibody Cocktail (#ab110411, Abcam).
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bioRxiv - Developmental Biology 2023Quote: 12μm thick cryosections of organoids were permeabilized in 0.1% Triton/PBS before incubation with primary antibodies at 1/200 dilution using appropriate serum as blocking buffer without antigen retrieval step: SOX2 (rabbit, ab92494 Abcam), TBR2 (sheep ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated with the following primary antibodies diluted in blocking buffer at room temperature for 2 hours: anti-OCT4 (1:200, catalog no. ab181557; Abcam); anti-TFAP2C (1:100 ...
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bioRxiv - Physiology 2023Quote: ... Tissue sections were incubated overnight at 4°C with a cocktail diluted in Buffer W (Nanostring; Seattle WA) containing morphology markers Desmin (Abcam, U.K ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were washed 3 times with PBS with 0.1% Tween-20 and were incubated for 1 hour in the following secondary antibodies diluted in blocking buffer (anti-guinea pig AF 405 1:750 (Abcam), anti-chicken AF 488 (Abcam) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 0.3% Triton X-100 and 90%PBS (Blocking Buffer) for 1 hr at room temperature and then incubated in primary antibodies (rabbit anti-Iba1, Abcam, ab178847 ...
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bioRxiv - Neuroscience 2023Quote: ... 2% goat serum) for 1h and then incubated overnight at 4°C in blocking buffer containing anti-PSER129 antibody EP1536Y (Abcam) diluted 1:50,000 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained in blocking buffer overnight at 4°C with the following primary antibodies at the indicated dilutions: anti-Gal-9 (Abcam ab275877 ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in permeabilization buffer (100 μl/106 cells) and incubated with anti-α-SMA antibody (1/1,000, [1A4] ab7817 Abcam) for 1h and then with Alexa Fluor® 488 or 647 secondary antibody (1/250 ...
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bioRxiv - Cancer Biology 2023Quote: ... The following primary antibody was incubated overnight at 4°C in blocking buffer: LINE-1 ORF1p (ab230966, Abcam, 1:200). Vector ImmPress anti-Rabbit HRP and ImmPact DAB kits (Vector Laboratories ...
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bioRxiv - Pathology 2023Quote: ... Slides were then subjected to heat-mediated antigen retrieval by placing slides in boiling Citrate buffer with a pH of 6.0 (Abcam, ab93678) and steamed for 30 minutes (100°C) ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were washed 3 times with PBS with 0.1% Tween-20 and were incubated for 1 hour in the following secondary antibodies diluted in blocking buffer (Anti-Guinea Pig AF 405 1:750 (Abcam), Anti-Chicken AF 488 (Abcam) ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunostaining was performed in saponin buffer (TBS supplemented with 1% BSA, 0.01% saponin) using the following antibodies: Rat anti-LAMP1 (Abcam #ab25245) at a concentration 1/400 ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was washed and incubated first in blocking buffer and then with an anti-m6A antibody (Abcam, ab151230, USA) overnight at 4 °C with gentle shaking ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonylation was detected by preparing skeletal muscle lysates from approximately 50 mg of muscle by homogenizing in lysis buffer provided by the Protein Carbonyl Content Assay Kit (Abcam). The resulting supernatants were subjected to western blotting following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... followed by staining with FITC-labelled anti-rabbit IgG (1:5000 diluted in 10 mL incubation buffer; ab6717, Abcam, UK). Then the chip was scanned by GenePix 4200A Microarray Scanner (Molecular Devices LLC ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 4% non-fat milk in PBS+0.1% Tween-20 for 1 h and incubated in the same buffer with rabbit anti-DHRS7 (Abcam ab156021) primary antibodies at 1:1,000 dilution for 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... The membranes then were incubated at 4◦C overnight with the primary antibodies diluted in the blocking buffer (rabbit anti-NLRP3 1:1000, Abcam, ab263899; rabbit anti-Gsdmd 1:1000, Abcam, ab219800 ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation at 4◦C overnight with primary antibodies diluted in the blocking buffer (rabbit anti-Dclk1 1:1000, Abcam, ab31704 ...
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bioRxiv - Immunology 2023Quote: ... The membranes then were incubated at 4◦C overnight with the primary antibodies diluted in the blocking buffer (rabbit anti-NLRP3 1:1000, Abcam, ab263899 ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation at 4◦C overnight with primary antibodies diluted in the blocking buffer (rabbit anti-Dclk1 1:1000, Abcam, ab31704; rabbit anti-Dclk1-488 1:1000, Abcam, ab202754 ...
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bioRxiv - Cancer Biology 2023Quote: ... and the cells were lysed in 80 μL of ice-cold buffer from Glutathione Assay Kit (catalog no: ab239709, Abcam). The lysed cells were kept on ice for 10 minutes followed by mixing thoroughly with 20 μL of 5% SSA (sulfosalicylic acid ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained overnight at 4 °C with primary antibody diluted in IFF buffer (anti-CD44 IM7, 1:500, eBioscience; anti-LGR5 OTI2A2, 1:200, Abcam; anti-GATA3 D13C9 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was accomplished by a 5-minute pressure cook of the slide submerged in 1X HIER buffer (abcam ab208572). Deionized water was slowly added to the pressure-cooked slide in HIER buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Human temporal cortex samples were lysed in CB Cell Lysis Buffer from the Cathepsin B Activity Assay Kit (Abcam, ab65300). Mouse cortex samples were lysed in RIPA buffer (150 mm sodium chloride ...
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bioRxiv - Genetics 2024Quote: ... Protein G beads (80 uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting H3K18ac (Abcam #ab40888), H3K18pr (PTM Bio #PTM-213) ...
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bioRxiv - Immunology 2024Quote: ... Unspecific binding sites were blocked as described above and then stained with anti-ARPC2 (1:50 in permeabilization buffer, Abcam) for 60 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellet was homogenized on ice with 150 µL of cold assay buffer containing 1 X protease inhibitor cocktail (Abcam), and centrifuged at 12,000 x g for 15 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]) ...
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bioRxiv - Pathology 2024Quote: ... Fixed cells were washed twice with FACS permeabilization buffer (DPBS(-/-) + 0.1% saponin) and labeled with mouse anti-sarcomeric alpha-actinin (Abcam, ab9465) for at least 1 hour at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The pellet was resuspended in 50 μL cold Antibody buffer with 0.5 μg of antibodies targeting H3K27ac (ab4729, Abcam, USA), or H3K27me3 antibodies and Rabbit IgG provided with the kit as positive and negative controls ...
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bioRxiv - Physiology 2024Quote: ... BAPTA-AM (to buffer [Ca2+]i; 1 µM for 20 min, from a 10 mM stock in dimethyl sulfoxide [DMSO], Abcam, with the concentration determined in preliminary experiments by titrating to a value that caused a ∼10 % decrease in percent sarcomere shortening during contraction) ...
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bioRxiv - Molecular Biology 2024Quote: ... for 2 h and then incubated overnight at +4°C with primary antibodies in 1:1 TBS and blocking buffer to analyze the content of pRLC (1:1000; ab2480, Abcam), Total OXPHOS Cocktail (OXPHOS ...
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bioRxiv - Cancer Biology 2024Quote: ... phyllodes and PanNET tissues were first disintegrated using a blue pestle and then lysed in 1% sodium dodecyl sulphate (SDS) hot lysis buffer following the hot lysis protocol (Abcam). SSTR-positive PanNET tissue was used as a positive control ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were blocked with BloxAll blocking buffer and 2.5% horse serum respectively prior to a 1-h incubation with anti-Ki67 antibody (Abcam, ab16667) at 1:100 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated for 72 hours at 4°C with the following dilutions of the primary antibodies in blocking buffer: Pecam1 1:50 (ab28364, Abcam), Keratin 8 1:100 (Troma1 ...
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bioRxiv - Developmental Biology 2023Quote: ... For anti-PI4KIIIalpha staining, the blocking buffer (for blocking, primary, and secondary steps) was supplemented with 5% Normal Goat Serum (Abcam).
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bioRxiv - Molecular Biology 2022Quote: ... The coding sequence of p21 was PCR amplified from flag-p21-WT(55) (a gift from Mien-Chie Hung, Abcam #16240) and cloned into pRetro-X-tight-PUR (Clontech) ...