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Citations for abcam :
201 - 250 of 542 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... coverslips were blocked for one hour at room temperature in blocking solution (Aqua block buffer, Abcam ab166952) and then transferred to a carrier solution (Aqua block ...
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bioRxiv - Cell Biology 2021Quote: ... followed by immunostaining with primary antibodies (diluted in blocking buffer) directed against NeuN (Abcam, #ab104224; 1:500), FABP7 (EMD Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were then diluted in BB blocking buffer (81A 1:500; D2G40 1:500; MAP2 Abcam #ab5392 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Frozen skeletal muscle was homogenized in the provided buffer with the Phosphoglucomutase activity assay kit (ab155896 abcam) supplemented with protease and phosphatase inhibitors (CIP treated samples contained only protease inhibitors) ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated overnight at 4°C in wash buffer containing Anti-CDKN2A/p16INK4a antibody (Abcam ab108349), Anti-LAMP1 antibody (Abcam ab25630) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells pre-incubated with NO dye and assay buffer 1 (Fluorometric Nitric Oxide Assay Kit, Abcam, UK) for 30mins at 37°C in 5% CO2 ...
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bioRxiv - Biochemistry 2019Quote: ... Deparaffinized and rehydrated 5-micron sections were subjected to antigen heat retrieval using citrate buffer (Abcam, ab208572), for 30 minutes at 100 °C ...
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bioRxiv - Immunology 2021Quote: ... Then cell lysate were cultivated with RIP buffer including anti-Ago2 (cat. no.186733; 1:1000; Abcam) and anti-IgG (cat ...
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bioRxiv - Cell Biology 2020Quote: Total cell lysates were obtained by resuspension of the cells in RIPA buffer (cat# ab 156034, Abcam) supplemented with proteinase and phosphatase inhibitors (cat# A32959 ...
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bioRxiv - Cell Biology 2022Quote: ... FFPE sections were rinsed with wash buffer and Podxl antibody applied for 30 minutes (Abcam, 1/1000). Sections were rinsed with wash buffer and rabbit envision secondary antibody applied for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The following primary antibodies used were diluted in blocking buffer: anti-Argonaute-2 (1:1000) (Abcam #32381), anti-Argonaute-1 (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: The ROs were pooled (n=10) and lysed in radioimmunoprecipitation assay (RIPA) protein lysis buffer (Abcam, UK) with protease inhibitor cocktail (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... They were incubated with the primary antibody (prepared in 1% BSA blocking buffer) (Fibrillarin - Abcam Cat#AB4566) overnight at 4°C ...
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bioRxiv - Genetics 2023Quote: ... The following primary antibodies were used diluted in blocking buffer– chicken anti-GFP (Abcam ab13970; 1:1000), mouse anti-PDF (DSHB-PDF C7 ...
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bioRxiv - Neuroscience 2023Quote: ... 50µl of reaction mix (47µl Glucose uptake assay buffer, 2µl of enzyme mix and 1µl of PicoProbe, all supplied by Abcam) was added to each sample and standard well ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was performed via heat-induced epitope retrieval (HIER) in universal antigen retrieval buffer (ab208572, Abcam). Deparaffinised tissue slides were immersed in antigen retrieval buffer which was brought to boil using a conventional microwave ...
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bioRxiv - Neuroscience 2023Quote: ... The nuclei were then stained with a buffer containing Anti-Erg 647 (1:200, Clone EPR3864, Abcam), Anti-NeuN Cy3 (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... and membranes were subsequently incubated overnight at 4°C in blocking buffer with anti-Sox2 antibodies (Abcam, Cambridge ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies were diluted in blocking buffer and applied overnight: rat anti-BrdU antibody (Abcam cat # Ab6326) was used to detect CldU ...
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bioRxiv - Molecular Biology 2024Quote: ... cell pellets were lysed in NP40 lysis buffer and RNAs were immunopurified using antibodies to Ago2 (Abcam) as previously described (42) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were harvested in RIPA buffer containing 100 µM PMSF and protease inhibitor cocktail (Abcam; Table S4). Protein extracts were then subjected to SDS-PAGE and subsequent immunoblotting as indicated in each figure.
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were then added in blocking buffer: anti-nestin [10C2] 1:200 (mouse monoclonal, Abcam, ab22035); anti-p(Th316)-nestin [a-4] at 1:200 (mouse monoclonal ...
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bioRxiv - Developmental Biology 2022Quote: ... The clones were analyzed for mutations by PCR and by Western Blot with anti-YAP antibody (Abcam, EP1674Y). To verify the out-of-frame indels the targeted locus was amplified and sequenced.
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bioRxiv - Biophysics 2020Quote: ... washed twice with PBS buffer (pH7.2) and developed by incubation with goat anti-mouse Ab-HRP conjugate (Abcam) (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibody was diluted in blocking buffer at the following dilutions: anti-CD34 (1:100; Abcam #MA1-10202), anti-FAPα (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... lysed using 62.5 mM Tris buffer (pH 6.8) containing 2% SDS and used for protein quantification using BCA assay (Abcam). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in odyssey blocking buffer for 1 hour and probed with streptavidin IR800 and anti-galectin 3 (Abcam). Membranes were imaged using a LI-COR Odyssey IR imager.
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bioRxiv - Cell Biology 2021Quote: ... buffers and stained by using 10 μg/ml of rabbit polyclonal anti-cleaved Caspase-3 (cat. ab13847, Abcam) antibody [or 10 μg/ml of rabbit IgG Isotype Control (cat ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were then incubated in blocking buffer 1 (BB1) consisting of 5% v/v donkey serum (abcam, ab7475), 1% v/v BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were first deparaffinized and antigen retrieval was performed using either citrate buffer or HIER solution (Abcam, ab208572). Successive steps were followed as previously described22 ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were diluted with the optimized diluting ratio in blocking buffer: Foxa2 (Abcam Cat# ab108422, RRID:AB_11157157,1:300), T (Cell Signaling Technology Cat# 81694 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We resuspended cell pellets in staining buffer containing propidium iodide (PI; dilution 1:100, ab14085, Abcam, Cambridge, UK) and incubated them for 10 minutes at room temperature in the dark ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were resuspended in 100 μL ChIP dilution buffer and incubated with 1 μg anti-HA (Abcam # ab9110) antibody for 2 h with constant rotation at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... The conjugated beads were then washed with SDS-Free RIPA buffer and incubated with either rCullin-1 (abcam) or 50μg of cell lysate ...
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bioRxiv - Physiology 2019Quote: ... followed by overnight incubation in the following primary antibodies diluted in Odyssey® Blocking Buffer: NDUFA9 (AB_301431, Abcam), mtCO1 (AB_2084810 ...
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bioRxiv - Biochemistry 2021Quote: ... then terminated by adding SDS loading buffer and subsequently subjected to western blot using anti-T7 antibody (Abcam) that was used to examinate fusion protein T7-Sic1PY.
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bioRxiv - Pathology 2021Quote: ... Deparaffinized and rehydrated 5-μm FFPE sections were subjected to antigen heat retrieval using citrate buffer (abcam, ab208572), in a pre-heated steam bath (100°C ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mL saponification buffer containing 7% KOH in 92% ethanol with 10 ug/mL coprostan-3-ol (Abcam) was added to 900 μL cell lysate ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were further washed with PBS and then washed two times with wash buffer (10% formamide (Abcam) in 2x saline sodium citrate (SSC) ...
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bioRxiv - Immunology 2021Quote: ... Primary antibodies were pre-diluted in blocking buffer to 1:200 for F4/80 (CI-A3-1; Abcam) or EpCAM (G8.8 ...
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bioRxiv - Immunology 2021Quote: ... Tissues from around 120 embryos were pooled and collected in lysis buffer provided by the kit (ab186032, Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and TMPRSS-2 protein (Rabbit Anti-Human Monoclonal, Citrate Buffer HIER, dilution 1:2500, Abcam, Code Number: ab242384) was assessed within the brainstem and cerebellum ...
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bioRxiv - Cell Biology 2023Quote: ... Antigen unmasking was performed by heating slides submerged in 1X Citrate buffer (pH 6.0) (cat. no. ab64214, Abcam) in a microwave for 1 minute at 100% power ...
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bioRxiv - Genomics 2023Quote: ... Primary antibody incubation was done in blocking buffer for 1 h at rt (anti PR Ser294Phospho, ab61785, Abcam). Primary antibody was washed 3 times in wash buffer (0.2% BSA ...
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bioRxiv - Immunology 2023Quote: ... Sections were then washed five times with PB buffer and blocked in 10 % normal goat serum (NGS; Abcam) at 4°C for 1.5 h ...
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bioRxiv - Immunology 2023Quote: ... Blots were stripped using a mild stripping buffer protocol (“Western blot membrane stripping for restaining protocol | Abcam,” n.d.), then re-blocked with 5% milk ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were resuspended in diluted kinase assay buffer containing 0.5 mM ATP and recombinant Aurora B kinase (AbCam). Kinase reactions were shaken at 30 °C for 10 mins at 1000 RPM and quenched with 4x LDS buffer (-EDTA ...
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bioRxiv - Microbiology 2023Quote: ... Cell lines were validated by phenotypic screening and confirmed to be mycoplasma-free using a PCR detection kit (Abcam). All cells were maintained at 37 °C in a 95% humidity and 5% CO2 atmosphere.
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bioRxiv - Cell Biology 2020Quote: ... Cell suspensions were centrifuged and resuspended in calcium-rich binding buffer provided by the apoptosis staining kit (abcam, #ab14085) to reach ~106 cells/mL ...