Labshake search
Citations for abcam :
2001 - 2050 of 2076 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Magnetic immunoprecipitation of sheared DNA-chromatin complexes (500ng) was performed overnight using a rabbit polyclonal histone H3K27acetyl ChIP Grade antibody (ab4729, Abcam), H3K27me3 (Active Motif ...
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bioRxiv - Biochemistry 2021Quote: ... and stained for SARS-CoV-2 N protein using Alexa488-labelled-CR3009 antibody produced in-house (see section for Recombinant mAb production) and cellular DNA using DRAQ7 (Abcam). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Molecular Biology 2020Quote: ... Labelled cells were harvested and DNA fibre spreads were incubated with rat anti-BrdU (BU1/75, Abcam ab6326, 1:700) and mouse anti-BrdU (B44 ...
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bioRxiv - Genetics 2023Quote: ... Lysates were sonicated to generate DNA fragments with sizes of 0.5 - 1 kb and immunoprecipitated with mouse monoclonal anti-H3K9me2 (abcam, ab1220), rabbit polyclonal anti-H3K9me3 (abcam ...
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bioRxiv - Biophysics 2023Quote: Equimolar mixture of histone octamer and LIN28B DNA (2 µM each) were mixed with 1 µM of Oct4 (abcam ab134876) and 3 µM of Sox2 (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... KIF1C-KO cell lines identified by DNA sequencing were verified for loss of KIF1C expression by Western Blot using Rb anti- KIF1C (Abcam) antibody.
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bioRxiv - Cancer Biology 2023Quote: ... DNA was denatured with 2.5 M HCl for 1 h and incubated with 1:1000 rat anti-BrdU (Abcam, ab6326) for 1 h at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5% vol/vol Triton X-100 in PBS) for 30 min and then incubated with 1 μg/mL primary antibody against DNA polymerase I (ab188424, Abcam) in blocking buffer for 60 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and labelled for amyloid precursor protein (APP) (Chemicon, 1:1000), MAC3 (Pharmingen, 1:400), glial fibrillary acidic protein (GFAP) (Novo Castra, 1:200) or IBA1 (abcam, 1:1000). Images were acquired at 40x magnification using a bright-field light microscope (Zeiss AxioImager Z1 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C overnight in the primary antibodies: chicken anti-glial fibrillary protein GFAP (targeting astrocytes, 1:200, Abcam #ab4674, USA), goat anti-ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C for 5-7 days in the primary antibodies containing: chicken anti-glial fibrillary acidic protein (GFAP) (targeting astrocytes, 1:200, Abcam #ab4674, USA) and/or and NeuN (targeting nuclei of neurons ...
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bioRxiv - Neuroscience 2021Quote: ... the brain slides were incubated with primary antibody rabbit monoclonal anti-amyloid precursor protein (APP) [clone Y188] (#AB32136, Abcam, USA; 1:500) in blocking solution at 4 °C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then washed thrice with 1X-TBST and incubated with anti-SARS-CoV-2 N protein antibodies (catalogue No. ab272852, Abcam, 1:1000) in TBST with BSA (2% ...
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bioRxiv - Molecular Biology 2021Quote: ... autoradiography recorded overnight using a phospho-imaging system and protein bands detected by Western blot using anti-TRIM25 (ab167154, Abcam, 1:2000).
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against nuclear proteins such as CENP-A (1:100 in PBS; o/n at 4°C + 2 hr at 37°C) (Abcam; ab45694, USA), lamin A/C (1:100 in PBS ...
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bioRxiv - Pathology 2019Quote: ... Soluble proteins (20 μg) were used for Western blotting with primary antibodies against AKT1/2/3 (rabbit monoclonal antibody; EPR16798 Abcam; 1:10,000), mTOR (rabbit monoclonal antibody ...
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bioRxiv - Neuroscience 2019Quote: ... Visualized neurons were labeled for chicken ovalbumin upstream promoter transcription-factor interacting protein-2 (CTIP2, 1:1000, rat, Abcam, Cat#:ab14865, RRID:AB_2064130) and pre-proenkephalin (PPE ...
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bioRxiv - Neuroscience 2020Quote: ... cell lysates were collected in parallel with the supernatants from the same wells and then tested for levels of a housekeeping protein beta-actin (Ab8227, Abcam, RRID: AB_2305186) by western blot ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then fixed with 4% paraformaldehyde and processed for immunofluorescence analysis using GAP SH3 Binding Protein 1 (G3BP1) antibody (green; Abcam, Cambridge, MA) and fluorophore-conjugated secondary antibody ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Astrogliosis was visualized after staining with rabbit Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody at a 1:1000 dilution (Abcam, Cambridge, MA). All samples were analyzed on an inverted epifluorescent microscope (Olympus IX71) ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated over night with primary antibody rabbit-anti-Ca2+/calmodulin-dependent protein kinase II (1:200, #PA5-38239, Thermo Fisher, MA, USA; 1:500, #ab52476, Abcam, UK), rabbit-anti-parvalbumin (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... and analyzed by western blot using anti-HBP1 (11746-1-AP, Protein Tech Group, 1:500) or anti-ZMYND19 antibody (ab86555, Abcam, 1:500).
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bioRxiv - Immunology 2020Quote: ... and equal amounts of fragmented chromatin–protein complexes were incubated for 2 h with enclosed Dynabeads coupled to rabbit anti-Polβ (Ab26343, Abcam, Cambridge, UK), mouse anti-MSH2 mAb (FE11 ...
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bioRxiv - Neuroscience 2020Quote: Lysates from DRG neurons were run on 4-12% Bis-Tris gradient gels and proteins were transferred to PVDF membranes that were incubated with antibodies to REST (Abcam, 1:1000), using anti-β-actin as a loading control ...
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bioRxiv - Biophysics 2021Quote: ... threonine and tyrosine phosphorylation of the wt and mutant T654A proteins was detected by western blotting using rabbit anti-pT654 (ab75986; Abcam, Cambridge, UK) and rabbit anti-pY1086 antibody (#4407 ...
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bioRxiv - Cell Biology 2022Quote: ... The relative amount of each of these muscle proteins in each lane was normalized to the amount of Histone H3 detected using anti-Histone H3 (abcam, cat. ab1791) at 1:40,000 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... or GT1-7MC4R cells (50 μg of protein) were subjected to immunoprecipitation overnight with 1 μg of a specific α2 AMPK antibody (ab3760, Abcam, Cambridge, MA) coupled with protein G-Sepharose beads (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... tissue proteins (20 μg) were separated by 4-20% SDS-PAGE and immunoblots were developed using antibodies against pSMAD3 (Abcam, 1:1000), SMAD3 (Abcam ...
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bioRxiv - Biochemistry 2019Quote: ... 20 μg of total cell lysates were separated on SDS-PAGE and protein transferred to nitrocellulose membranes that were probed with antibodies against human pancreatic α-amylase (Abcam #ab21156) and ENT2 (Alomone Labs #ANT-052 ...
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bioRxiv - Biochemistry 2021Quote: ... The homogenate was clarified by centrifugation at 21,130 x g for 15 minutes before analysis of protein levels in the supernatant by immunoblotting using antibodies including anti-PKA pRIIα (Abcam, RRID: AB_779040), anti-GFP (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for intracellular spike protein using an Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (Abcam, ab275759, 1:50) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a pair of monoclonal antibodies (clone EPR16588, ab178846; clone EPR16589, ab178847) and a recombinant human protein (ab105593) are available from Abcam (Waltham, MA). Similarly ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... was used for indirect immunofluorescence microscopy at a dilution of 1:300 and mouse monoclonal antibody against nuclear pore complex proteins (Abcam, Cambridge, MA) was used for indirect immunofluorescence microscopy at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Electroporated cells were seeded in StemMACS iPS-Brew XF with Rho-associated protein kinase (ROCK) inhibitor Y-27632 (10 μM, Abcam, Cambridge, UK) and 1x CloneR (Stemcell Technologies ...
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bioRxiv - Immunology 2022Quote: ... or CD8+ cells using a rabbit-raised anti-SARS-CoV-2 S protein antibody (1:5000 dilution, ab272504, Abcam plc, Cambridge UK), a rabbit monoclonal anti-CD4 antibody (1:250 dilution ...
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bioRxiv - Microbiology 2022Quote: ... paraffin-embedded sections were firstly subjected to immunostaining with a commercially available and diluted (1:5,000) rabbit-anti-SARS-CoV-2 spike (S) protein (ab272504, Abcam Plc., Cambridge, UK), followed by staining with a peroxidase-conjugated secondary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant calibrated into the same protein concentration was used for the measurement of oxytocin levels using an ELISA kit (Abcam, lot. 133050), according to the manufacturer’s instructions.
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Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cellsbioRxiv - Neuroscience 2022Quote: The following primary antibodies were used in this study: two rabbit polyclonal antibodies against human fibronectin leucine-rich transmembrane protein 1 (FLRT1) (ab97825 and ab103839; Abcam, Cambridge, UK), rabbit polyclonal anti-human FLRT2 (ab154023 ...
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bioRxiv - Molecular Biology 2022Quote: Nuclear protein isolations from frozen muscle tissue were performed using a commercial kit (Nuclear Extraction Kit, catalog# ab113474; Abcam; Waltham, MA, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... They were then washed with PBS and incubated with 1° antibodies (α-NDUFV2, 15301-1-AP, Protein tech, α-tubulin, Abcam, ab179513) in 5% milk 0.1% Tween 20 ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of protein of EV or lysate samples were then subjected to western blot analysis for ALIX (ab186429; Abcam, Cambridge, UK) at 1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed on top of a microscope slides and DNA fibers were spread by tilting the microscope slide and CldU was detected with rat anti-BrdU (Abcam, ab6326), whereas IdU was detected with mouse anti-BrdU (BD Biosciences ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies for DNA fibers were used as follows: for the tracts labeled with CldU (anti-BrdU, rat, Abcam, ab6326, 1:100) and IdU (anti-BrdU mouse ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were rinsed with PBS three times before and after staining the nuclear DNA with NucBlue and Phalloidin-iFluor 647(Abcam, ab176759) for 30 minutes at room temperature.
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bioRxiv - Cell Biology 2020Quote: ... Co-immunoprecipitation of α-tubulin interacting proteins was performed from total cell lysates using α-Tubulin specific antibodies (rabbit anti-α-tubulin, ab52899, Abcam) and Protein A/G Sepharose beads (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2020Quote: ... The eluates were boiled with LDS protein loading buffer (Thermo, NP0008) and loaded on NuPAGE for gel electrophoresis and Western blotting with antibody to ANT1 (Abcam, ab102032, 1:3000). In some experiments ...
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bioRxiv - Genetics 2021Quote: ... ran side by side in a Criterion Precast Gradient 4-15% SDS-PAGE gel for western blotting probing of key intracellular organellar markers and proteins of interest: HSP60 (Abcam, cat. number ab46798), Calnexin (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... with 2-8 μg total protein loaded per lane and the following dilutions of mouse mAbs: anti-K14 (1:500; clone LL002, Abcam Inc., Cambridge, UK), anti-K42 (1:500) ...