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Citations for abcam :
1601 - 1650 of 2076 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: The relative contents of endogenous sphingomyelins in WT and SIRT1 KO mESCs were analyzed using a commercially available Sphingomyelin Assay Kit (Abcam ab133118) per manufacturer’s instruction.
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bioRxiv - Genetics 2020Quote: ... Freshly isolated fibroblasts lysates were used to measure cathepsin D activity using a fluorometric plate reader assay per manufacturer instructions (abcam ab65302).
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bioRxiv - Microbiology 2022Quote: ... The specificity of the Enzchek Ultra Amylase kit was tested using control enzymes from the α-Glucosidase Activity Assay Kit (Abcam), an αmylase kit (Abcam ...
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bioRxiv - Neuroscience 2023Quote: Conditioned media from the somatic side of the same MFCs used for the ELISA was analysed with the Cytotoxicity LDH assay kit (Abcam #ab65393) following manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: RalGDS RBD Agarose beads were used to selectively pull-down the active form of Rap1 (Rap-GTP) from isolated cardiac mitochondria (Rap1 Activation Assay Kit, Abcam, ab212011). Samples of mitochondria ...
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bioRxiv - Cell Biology 2023Quote: Detection of cytoplasmatic and mitochondrial ROS levels was performed by means of a commercial DCFDA / H2DCFDA - Cellular ROS Assay Kit (abcam, ab113851) and MitoSOX™ Red Mitochondrial Superoxide Indicator (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The preparation of samples and lactate level measurement procedure were followed as specified in the Lactate Assay Kit (Colorimetric) (ABCAM - ab65331) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The measurement of Cathepsin L activity was performed following the instructions and the protocol of Magic Red Cathepsin Assays Kit (Abcam, ab270774). Photos were taken using a Floid Cell Imaging Station (Thermo Fisher Scientist) ...
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bioRxiv - Molecular Biology 2023Quote: LAL activity was determined in control and VPS35-deficient Hepa1-6 cells using the LysoLive™ Lysosomal Acid Lipase Assay Kit (ab253380, Abcam) according to the manufacturer’s instructions for adherent cells ...
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bioRxiv - Immunology 2023Quote: ... Thymocytes were isolated from untreated and TBI-treated mice and intracellular pyruvate and lactate levels were measured by absorbance using Pyruvate Assay kit (Abcam, ab65342) or Lactate-Glo™ Assay (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Antibodies used for immunostaining of plaque assays are: monoclonal anti-SARS-CoV-NP antibody (1C7C7) and anti-mouse HRP antibody (Abcam ab6823). Antibodies used for immunostaining of histology slides are ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were diluted 1:400 in Milli-Q water and lactate concentration was measured using a Lactate assay kit (Abcam, ab65330) according to the manufacturer’s protocol (using 1.5 µL of Enzyme Mix per well) ...
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bioRxiv - Neuroscience 2023Quote: Measurement of the oxygen consumption rate (OCR) in hiPSC-derived neurons was performed with the fluorescence-based Extracellular O2 Consumption Assay Kit (Abcam, ab197243) as described previously (Castelo Rueda et al. ...
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bioRxiv - Microbiology 2023Quote: ... we measured β-1,3-glucan with an assay that first specifically hydrolyzes β-1,3-glucan and then detects liberated glucose with a fluorescent probe (abcam 303728). 1.25 µL of AI or AS material was used per reaction and all reactions were performed in triplicate ...
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bioRxiv - Bioengineering 2023Quote: ... 1 mg/ml collagen gels were incubated with 10mM MGO and the clinically relevant MGO-derived advanced glycation end-products (AGE) hydroimidazolone MG-H1 was measured using a competitive ELISA assay (Abcam, UK) according to the manufacturer’s instructions and previously published approaches23.
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bioRxiv - Biochemistry 2023Quote: ... The lipid fraction from 1 million cells per sample was extracted after which total cholesterol was assayed using the Cholesterol/Cholesteryl Ester Quantitation Assay kit (Abcam, ab65359) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Infected cell lines were incubated at 37°C in a carbogen environment for two hours prior to conducting cytotoxicity assays using a lactate dehydrogenase (LDH) kit (Abcam, UK) as previously described(48) ...
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bioRxiv - Cancer Biology 2023Quote: To quantitatively measure intracellular reactive oxygen species in live cell samples we have used cell permeant reagent 2’,7’–dichlorofluorescin diacetate (H2DCFDA) from Cellular ROS Assay Kit (Abcam, ab113851) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: For measuring cellular iron in Fe2+ and Fe3+ states as well as total iron content we have a used a colorimetric Iron Assay Kit system (Abcam, ab83366) in stepwise manner as described in the manufacture’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: For the assessment of cell death-induced DNA fragmentation of lung cells the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed according to the manufacturer’s instructions (ab66110, Abcam, Cambridge, Massachusetts) on paraffin-embedded lung tissue sections with 3 µm thickness ...
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bioRxiv - Cancer Biology 2023Quote: ... The pellet was centrifuged once again and re-suspended in nuclear extraction buffer from the kit to generate the nuclear fraction and samples used for the NF-κB p65 transcription ELISA based assay (Abcam, ab133112) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed twice with cold PBS and resuspended in the assay buffer provided for homogenization according to the manufacturer’s instructions (Abcam, Cat# ab83355). The supernatant of the cell lysates was incubated with ATP reaction mix for 30 min followed by fluorescence measurements using Synergy H1 plate-reader with excitation/emission 535/587 nm.
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bioRxiv - Cancer Biology 2024Quote: ... ROS indicator dye was prepared according to the manufacturer’s protocol using DCFDA/H2DCFDA - Cellular ROS Assay Kit (Abcam, ab113851, Cambridge, UK), and was added to each well ...
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bioRxiv - Cell Biology 2024Quote: The mitochondrial membrane potential (ΔΨm) was measured with the JC-1 mitochondrial membrane potential assay kit (ab113580, Abcam, Cambridge, United Kingdom) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and analyzed by Western blotting using antibodies recognizing the following proteins: AAG (Abcam ab155092, 42KD, 1:5000), beta-actin (Sigma A5441 ...
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bioRxiv - Bioengineering 2019Quote: ... the antibodies specific to these proteins (Anti-Nesprin-2 ab57397 and anti-Lamin-A ab8980, Abcam, UK) were used according to manufacturer’s directions ...
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bioRxiv - Systems Biology 2019Quote: ... Bound proteins were resolved by SDS-PAGE and detected using an αMYC-tag HRP-conjugated antibody (Abcam).
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Inhibiting the prostaglandin transporter PGT induces non-canonical thermogenesis at thermoneutralitybioRxiv - Physiology 2019Quote: ... Tissue protein kinase A (PKA) activity was measured according to the manufacturer’s instructions (Abcam #ab139435, Cambridge, MA). Stool non-esterified free fatty acid content was measured colorimetrically (HR Series NEFA-HR(2) ...
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bioRxiv - Microbiology 2019Quote: ... and with or without 10 μM Rho-associated protein kinase inhibitor (Y-27632, Abcam, Cambridge, United Kingdom). For establishment of well-differentiated airway epithelial cell cultures ...
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bioRxiv - Neuroscience 2020Quote: ... antibodies against the following proteins were used at the indicated dilutions: Mouse anti-RLBP1 (1:500, Abcam), Mouse anti-Rod-OPSIN (1:1 000 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was detected with the primary antibodies anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) (Abcam, ab92696), anti-PDK1 (Cell Signaling Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... The rabbit primary Abs against the following proteins were used: Artemin and S100β (Abcam, Cambridge, United Kingdom), and β-actin (Abclonal ...
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bioRxiv - Neuroscience 2020Quote: Biochemical analysis of protein content by Western blot used the following antibodies: Numb (cat. no. ab4147; Abcam), Eps15 (cat ...
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Axon-secreted chemokine-like Orion is a signal for astrocyte infiltration during neuronal remodelingbioRxiv - Neuroscience 2020Quote: ... The Orion-Myc and Tubulin proteins were detected using a mouse anti-Myc antibody (clone 9E10, AbCam) and an anti-Tubulin antibody (Sigma ...
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bioRxiv - Physiology 2021Quote: ... Protein expression was analyzed using the following primary antibodies: mouse PDK4 (Abcam, catalog number ab110336, 1:1000), rabbit PDH (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... Before re-probing for the housekeeping protein GAPDH (mouse monoclonal [6C5] diluted at 1:5000; Abcam #8245) according to the same protocol but using goat anti-mouse HRP-conjugated secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: After blocking, proteins were exposed to primary antibodies overnight at 4oC (anti-γ2 phospho-S327, ab73183 (Abcam), 1:1000) ...
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bioRxiv - Immunology 2022Quote: ... Sections were then covered with rabbit anti-SARS-CoV-2 N protein monoclonal antibody (Abcam; 1:1000) for 1 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Anti-Myelin Basic Protein (MBP)-rabbit polyclonal antibody and anti-Ki67 (SP6) unconjugated was purchased from Abcam.
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bioRxiv - Neuroscience 2020Quote: ... Blocked protein blot was incubated with affinity-purified rabbit anti-ACE2 (1:1000, Abcam, Cat No 15348), and mouse anti-b-actin (1:5000 ...
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bioRxiv - Immunology 2022Quote: ... 500 μg of protein lysate was incubated with Anti-6X His tag® antibody [HIS.H8] (ab18184, Abcam) overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein-A magnetic beads (100ul) were blocked with 0.5% BSA and coated with the 5mg antibody (Abcam; PRDM1 ab13700 ...
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bioRxiv - Biochemistry 2019Quote: ... The high-affinity/sensitivity/specificity mouse anti valosin-containing protein (anti VCP) antibody (catalog number ab11433; Abcam) was used for multiplex Western blotting as a loading control ...
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bioRxiv - Molecular Biology 2020Quote: ... Each elution fraction was resolved on an SDS-PAGE gel stained with InstantBlue Coomassie Protein Stain (Abcam), and fractions containing a single RBD band were pooled ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to PVDF membranes and immunoblotted with α-GBF1 (ab86071; abcam, at 1:1000 dilution) and HRP conjugated α-FLAG (A8592 ...
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bioRxiv - Systems Biology 2019Quote: ... Isolation and protein extraction of mitochondria was performed by Mitochondria Isolation Kit for Cultured Cells (ab110170. abcam). Proteins were separated by Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Genomics 2021Quote: ... and loading control protein ATP5A antibody (1:1.000 anti-ATP5A antibody, cat no. ab151229, Abcam, Cambridge, UK). After the primary antibody treatments ...
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bioRxiv - Cell Biology 2021Quote: ... HIF-1α protein was extracted from cell pellets and confirmed using Human Simple Step ELISA kit (Abcam) according to manufacturer’s instructions.